Mutagenetix > Incidental Mutation

Incidental Mutation 'R4812:Dctn1'

369538

Institutional Source

Beutler Lab

Gene Symbol

Dctn1

Ensembl Gene

ENSMUSG00000031865

Gene Name

dynactin 1

Synonyms

p150, Glued, p150

MMRRC Submission

042431-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4812 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

83142902-83177099 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 83166919 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 160 (M160L)

Ref Sequence

ENSEMBL: ENSMUSP00000109540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077407] [ENSMUST00000113907] [ENSMUST00000113913] [ENSMUST00000113918] [ENSMUST00000113919] [ENSMUST00000141680] [ENSMUST00000130212]

AlphaFold

O08788

Predicted Effect

probably benign
Transcript: ENSMUST00000077407
AA Change: M257L

PolyPhen 2 Score 0.083 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000076623
Gene: ENSMUSG00000031865
AA Change: M257L

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	124	147	N/A	INTRINSIC
low complexity region	148	177	N/A	INTRINSIC
SCOP:d1fxkc_	185	337	3e-3	SMART
low complexity region	363	379	N/A	INTRINSIC
Pfam:Dynactin	489	768	8.2e-91	PFAM
low complexity region	800	820	N/A	INTRINSIC
coiled coil region	914	1009	N/A	INTRINSIC
low complexity region	1025	1043	N/A	INTRINSIC
coiled coil region	1143	1172	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113907
AA Change: M160L

PolyPhen 2 Score 0.237 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000109540
Gene: ENSMUSG00000031865
AA Change: M160L

Domain	Start	End	E-Value	Type
low complexity region	5	22	N/A	INTRINSIC
low complexity region	27	50	N/A	INTRINSIC
low complexity region	51	80	N/A	INTRINSIC
low complexity region	93	106	N/A	INTRINSIC
low complexity region	140	158	N/A	INTRINSIC
SCOP:d1lxa__	271	345	8e-3	SMART
Pfam:Dynactin	392	671	7.1e-91	PFAM
low complexity region	703	723	N/A	INTRINSIC
coiled coil region	817	912	N/A	INTRINSIC
low complexity region	928	946	N/A	INTRINSIC
coiled coil region	1046	1075	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000113913
AA Change: M277L

SMART Domains

Protein: ENSMUSP00000109546
Gene: ENSMUSG00000031865
AA Change: M277L

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	118	139	N/A	INTRINSIC
low complexity region	144	167	N/A	INTRINSIC
low complexity region	168	197	N/A	INTRINSIC
SCOP:d1fxkc_	205	357	3e-3	SMART
low complexity region	383	399	N/A	INTRINSIC
Pfam:Dynactin	509	788	2.5e-90	PFAM
low complexity region	820	840	N/A	INTRINSIC
coiled coil region	934	1029	N/A	INTRINSIC
low complexity region	1051	1069	N/A	INTRINSIC
coiled coil region	1168	1197	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113918
AA Change: M294L

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000109551
Gene: ENSMUSG00000031865
AA Change: M294L

Domain	Start	End	E-Value	Type
CAP_GLY	29	95	5.52e-31	SMART
low complexity region	135	156	N/A	INTRINSIC
low complexity region	161	184	N/A	INTRINSIC
low complexity region	185	214	N/A	INTRINSIC
low complexity region	227	240	N/A	INTRINSIC
low complexity region	274	292	N/A	INTRINSIC
low complexity region	400	416	N/A	INTRINSIC
Pfam:Dynactin	526	805	3.3e-90	PFAM
low complexity region	837	857	N/A	INTRINSIC
coiled coil region	951	1046	N/A	INTRINSIC
coiled coil region	1147	1176	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113919
AA Change: M294L

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000109552
Gene: ENSMUSG00000031865
AA Change: M294L

Domain	Start	End	E-Value	Type
CAP_GLY	29	95	5.52e-31	SMART
low complexity region	135	156	N/A	INTRINSIC
low complexity region	161	184	N/A	INTRINSIC
low complexity region	185	214	N/A	INTRINSIC
SCOP:d1fxkc_	222	374	3e-3	SMART
low complexity region	400	416	N/A	INTRINSIC
Pfam:Dynactin	522	805	1.4e-103	PFAM
low complexity region	837	857	N/A	INTRINSIC
coiled coil region	951	1046	N/A	INTRINSIC
low complexity region	1068	1086	N/A	INTRINSIC
coiled coil region	1185	1214	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123313

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127824

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154420

Predicted Effect

probably benign
Transcript: ENSMUST00000141680

SMART Domains

Protein: ENSMUSP00000121538
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	118	139	N/A	INTRINSIC
low complexity region	144	159	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000130212

SMART Domains

Protein: ENSMUSP00000115838
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	124	147	N/A	INTRINSIC
low complexity region	148	177	N/A	INTRINSIC

Meta Mutation Damage Score

0.0709

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.2%

Validation Efficiency

93% (124/133)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality and developmental arrest at E7.5 associated with increased apoptosis. [provided by MGI curators]

Allele List at MGI

All alleles(20) : Targeted(4) Gene trapped(16)

Other mutations in this stock

Total: 128 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017G19Rik	C	T	3: 40,575,633 (GRCm39)		noncoding transcript	Het
Abca5	T	C	11: 110,192,647 (GRCm39)	D681G	probably damaging	Het
Actl11	T	C	9: 107,808,329 (GRCm39)	V884A	probably damaging	Het
Akr1c14	G	A	13: 4,129,165 (GRCm39)	V187M	probably damaging	Het
Apbb1	A	T	7: 105,223,232 (GRCm39)	N126K	probably damaging	Het
Bmt2	G	T	6: 13,677,799 (GRCm39)	R12S	unknown	Het
Btrc	G	A	19: 45,411,603 (GRCm39)	C9Y	possibly damaging	Het
C5ar1	A	C	7: 15,982,258 (GRCm39)		probably null	Het
C8a	C	T	4: 104,719,788 (GRCm39)		probably null	Het
Cabin1	A	G	10: 75,482,428 (GRCm39)	S2172P	possibly damaging	Het
Calcoco2	T	C	11: 95,998,276 (GRCm39)	D49G	probably damaging	Het
Camta1	C	T	4: 151,215,999 (GRCm39)	D974N	probably null	Het
Car6	T	C	4: 150,281,872 (GRCm39)	E47G	probably damaging	Het
Ccnd3	G	A	17: 47,908,505 (GRCm39)		probably null	Het
Celf5	A	T	10: 81,306,573 (GRCm39)	V30E	probably damaging	Het
Cfap251	G	A	5: 123,425,368 (GRCm39)	V776I	probably benign	Het
Cfap46	T	A	7: 139,215,916 (GRCm39)	D1513V	probably damaging	Het
Cinp	T	C	12: 110,846,174 (GRCm39)	Y84C	probably damaging	Het
Cnih1	A	G	14: 47,014,001 (GRCm39)	I154T	probably damaging	Het
Col15a1	C	T	4: 47,262,479 (GRCm39)	P511L	possibly damaging	Het
Col4a4	C	T	1: 82,439,874 (GRCm39)	V1364M	unknown	Het
Cplane1	T	G	15: 8,230,607 (GRCm39)		probably null	Het
Crtam	A	G	9: 40,895,621 (GRCm39)	L38P	probably damaging	Het
Ctnna1	T	A	18: 35,372,530 (GRCm39)	V495D	probably damaging	Het
Cubn	T	C	2: 13,463,887 (GRCm39)	Y606C	probably damaging	Het
Cyp4f16	C	T	17: 32,765,652 (GRCm39)	A345V	probably null	Het
Dip2c	T	A	13: 9,687,166 (GRCm39)	C366*	probably null	Het
Dnajc2	G	A	5: 21,968,484 (GRCm39)	S401L	probably benign	Het
Dnmt3l	A	G	10: 77,893,128 (GRCm39)	I302V	probably benign	Het
Dvl2	T	C	11: 69,902,119 (GRCm39)		probably benign	Het
Edn1	T	G	13: 42,457,116 (GRCm39)	S50A	probably benign	Het
Efhc1	A	T	1: 21,060,871 (GRCm39)	R636W	probably damaging	Het
Epg5	T	A	18: 78,022,399 (GRCm39)	H1047Q	probably benign	Het
Etv1	T	G	12: 38,911,287 (GRCm39)	V371G	probably damaging	Het
Fap	T	A	2: 62,349,365 (GRCm39)	I475F	probably damaging	Het
Fbxw15	T	C	9: 109,388,990 (GRCm39)	I140V	probably benign	Het
Fer	A	G	17: 64,241,292 (GRCm39)	T311A	probably benign	Het
Fh1	A	G	1: 175,429,025 (GRCm39)	W497R	probably damaging	Het
Flot2	T	A	11: 77,944,191 (GRCm39)	L45Q	probably damaging	Het
Flt1	C	A	5: 147,620,749 (GRCm39)	A132S	probably benign	Het
Fmnl1	T	C	11: 103,089,390 (GRCm39)		probably benign	Het
Garin5b	G	A	7: 4,762,071 (GRCm39)	T295M	probably damaging	Het
Ggta1	C	T	2: 35,292,735 (GRCm39)	V203I	probably benign	Het
Gm19345	C	T	7: 19,591,798 (GRCm39)	V204M	probably damaging	Het
Gm4204	T	A	1: 135,160,227 (GRCm39)		noncoding transcript	Het
Gm6124	C	G	7: 38,872,319 (GRCm39)		noncoding transcript	Het
Gprin3	T	A	6: 59,330,350 (GRCm39)	K652N	possibly damaging	Het
Gucy1b2	T	C	14: 62,653,346 (GRCm39)		probably null	Het
Hace1	C	A	10: 45,562,699 (GRCm39)	A738E	probably benign	Het
Hectd1	C	T	12: 51,874,134 (GRCm39)		probably null	Het
Hnrnpdl	T	C	5: 100,184,331 (GRCm39)		probably benign	Het
Hyou1	T	C	9: 44,298,418 (GRCm39)		probably benign	Het
Ifi44l	G	A	3: 151,465,336 (GRCm39)	A138V	probably benign	Het
Igkv16-104	A	T	6: 68,402,829 (GRCm39)	I41F	possibly damaging	Het
Ints7	T	A	1: 191,326,542 (GRCm39)	D171E	possibly damaging	Het
Irag2	G	A	6: 145,093,737 (GRCm39)	G120S	probably damaging	Het
Kmt2a	A	T	9: 44,742,651 (GRCm39)		probably benign	Het
Kmt2e	T	C	5: 23,707,585 (GRCm39)	V1716A	possibly damaging	Het
Krtap5-1	G	A	7: 141,850,628 (GRCm39)	S60F	unknown	Het
Lama4	G	A	10: 38,948,765 (GRCm39)	V843I	probably benign	Het
Laptm5	A	G	4: 130,640,749 (GRCm39)		probably null	Het
Lbhd1	G	A	19: 8,866,538 (GRCm39)	A193T	probably damaging	Het
Lce3f	C	T	3: 92,900,247 (GRCm39)	P23S	unknown	Het
Mecom	A	C	3: 30,194,517 (GRCm39)	M1R	probably null	Het
Mindy4	A	C	6: 55,256,088 (GRCm39)	T531P	possibly damaging	Het
Mrpl3	A	G	9: 104,951,023 (GRCm39)	N263S	probably damaging	Het
Myo18b	T	C	5: 112,957,584 (GRCm39)	K1460E	possibly damaging	Het
Myof	A	G	19: 37,905,007 (GRCm39)	Y852H	probably damaging	Het
Nefl	T	G	14: 68,321,734 (GRCm39)	V108G	probably damaging	Het
Nid1	T	A	13: 13,681,053 (GRCm39)	L1061*	probably null	Het
Nim1k	T	A	13: 120,173,920 (GRCm39)	M325L	probably benign	Het
Nlrp1c-ps	T	C	11: 71,143,131 (GRCm39)		noncoding transcript	Het
Npsr1	C	T	9: 24,201,252 (GRCm39)	T59I	probably damaging	Het
Nr2c1	A	G	10: 94,024,114 (GRCm39)	T440A	probably benign	Het
Nup160	C	T	2: 90,556,035 (GRCm39)	T1245I	probably damaging	Het
Nup88	T	A	11: 70,856,552 (GRCm39)	T194S	probably damaging	Het
Oas3	A	G	5: 120,899,212 (GRCm39)		probably benign	Het
Odad2	T	G	18: 7,288,634 (GRCm39)	T78P	possibly damaging	Het
Opn1sw	A	T	6: 29,378,038 (GRCm39)	M252K	probably damaging	Het
Oprm1	T	G	10: 6,782,698 (GRCm39)		probably benign	Het
Or10ag59	A	G	2: 87,406,087 (GRCm39)	I220V	probably benign	Het
Or2y1g	T	A	11: 49,171,284 (GRCm39)	I103K	possibly damaging	Het
Or4x13	T	A	2: 90,231,440 (GRCm39)	M145K	probably benign	Het
Pcdha2	T	A	18: 37,072,861 (GRCm39)	V164E	probably benign	Het
Pclo	A	G	5: 14,590,039 (GRCm39)	T780A	unknown	Het
Pcnx2	T	A	8: 126,592,678 (GRCm39)	Q762L	probably benign	Het
Pcyt2	A	T	11: 120,505,251 (GRCm39)		probably benign	Het
Pdpr	A	G	8: 111,843,349 (GRCm39)	N294D	probably benign	Het
Perm1	T	A	4: 156,303,193 (GRCm39)	V579E	possibly damaging	Het
Pgk2	T	A	17: 40,518,281 (GRCm39)	K382N	possibly damaging	Het
Plcb4	T	A	2: 135,849,801 (GRCm39)	L205Q	probably damaging	Het
Plekha3	C	T	2: 76,516,975 (GRCm39)	T109I	probably damaging	Het
Pnn	T	A	12: 59,118,404 (GRCm39)	V329E	possibly damaging	Het
Ptpn13	A	G	5: 103,671,481 (GRCm39)	I469M	probably benign	Het
Rapgef3	A	G	15: 97,651,684 (GRCm39)	V603A	probably benign	Het
Rbms1	C	T	2: 60,623,113 (GRCm39)	V75I	possibly damaging	Het
Rbp3	T	A	14: 33,676,731 (GRCm39)	D226E	probably damaging	Het
Robo2	A	T	16: 73,713,176 (GRCm39)	N1189K	probably benign	Het
Rragd	A	G	4: 33,018,766 (GRCm39)	T270A	probably benign	Het
Rxfp1	T	A	3: 79,557,889 (GRCm39)	T530S	probably benign	Het
Ryr3	T	C	2: 112,742,581 (GRCm39)	E479G	probably damaging	Het
Scd2	A	T	19: 44,289,841 (GRCm39)	I279F	probably damaging	Het
Sh3d19	A	T	3: 86,031,074 (GRCm39)	D746V	probably damaging	Het
Shroom4	A	G	X: 6,536,180 (GRCm39)	K1133E	probably benign	Het
Sirpb1c	A	G	3: 15,887,386 (GRCm39)	V151A	probably damaging	Het
Slc26a2	A	T	18: 61,335,093 (GRCm39)	I120N	probably damaging	Het
Slco1a1	A	G	6: 141,864,319 (GRCm39)	S494P	probably damaging	Het
Srebf2	A	T	15: 82,088,026 (GRCm39)	T1061S	probably damaging	Het
Sspo	T	C	6: 48,467,444 (GRCm39)	L4202P	probably benign	Het
Synj2	G	A	17: 6,060,939 (GRCm39)	G215E	probably damaging	Het
Tbc1d19	T	C	5: 53,967,148 (GRCm39)	V16A	probably damaging	Het
Tektl1	T	C	10: 78,585,050 (GRCm39)	H262R	probably benign	Het
Tiparp	T	C	3: 65,460,190 (GRCm39)	I495T	possibly damaging	Het
Tipin	A	G	9: 64,211,694 (GRCm39)	H260R	probably benign	Het
Tle1	A	G	4: 72,063,591 (GRCm39)	S221P	probably damaging	Het
Tmem94	T	A	11: 115,686,938 (GRCm39)	L1101*	probably null	Het
Trim43b	C	T	9: 88,973,533 (GRCm39)	D67N	probably benign	Het
Ubn2	T	A	6: 38,440,661 (GRCm39)	C178S	probably benign	Het
Vmn1r54	T	C	6: 90,246,307 (GRCm39)	F74L	probably benign	Het
Vmn2r24	A	G	6: 123,756,144 (GRCm39)	H72R	probably benign	Het
Wnk1	C	T	6: 119,929,732 (GRCm39)	V850I	probably benign	Het
Zan	T	G	5: 137,454,547 (GRCm39)	Y1419S	unknown	Het
Zbtb14	T	A	17: 69,694,577 (GRCm39)	Y92N	probably damaging	Het
Zfand5	A	G	19: 21,255,101 (GRCm39)	K116E	probably benign	Het
Zfhx3	A	G	8: 109,674,593 (GRCm39)	E1881G	possibly damaging	Het
Zfp280b	A	G	10: 75,874,924 (GRCm39)	K268E	probably benign	Het
Zfp541	A	G	7: 15,813,035 (GRCm39)	I563V	probably benign	Het
Zfy2	T	A	Y: 2,106,334 (GRCm39)	I767L	probably benign	Het

Other mutations in Dctn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01113:Dctn1	APN	6	83,156,879 (GRCm39)	missense	probably benign	0.00
IGL01450:Dctn1	APN	6	83,171,092 (GRCm39)	unclassified	probably benign
IGL01876:Dctn1	APN	6	83,174,903 (GRCm39)	missense	probably damaging	1.00
IGL01958:Dctn1	APN	6	83,168,326 (GRCm39)	missense	possibly damaging	0.95
IGL02554:Dctn1	APN	6	83,159,704 (GRCm39)	missense	probably damaging	1.00
IGL02668:Dctn1	APN	6	83,168,030 (GRCm39)	missense	possibly damaging	0.89
IGL02814:Dctn1	APN	6	83,166,896 (GRCm39)	missense	probably damaging	1.00
IGL02818:Dctn1	APN	6	83,169,496 (GRCm39)	missense	possibly damaging	0.86
IGL03007:Dctn1	APN	6	83,159,690 (GRCm39)	missense	probably damaging	1.00
IGL03065:Dctn1	APN	6	83,169,475 (GRCm39)	missense	probably damaging	0.99
IGL03083:Dctn1	APN	6	83,174,466 (GRCm39)	splice site	probably benign
IGL03394:Dctn1	APN	6	83,168,266 (GRCm39)	missense	possibly damaging	0.61
E0374:Dctn1	UTSW	6	83,171,156 (GRCm39)	missense	possibly damaging	0.93
IGL03014:Dctn1	UTSW	6	83,174,351 (GRCm39)	intron	probably benign
PIT4812001:Dctn1	UTSW	6	83,176,744 (GRCm39)	missense	possibly damaging	0.86
R0044:Dctn1	UTSW	6	83,168,116 (GRCm39)	missense	probably damaging	1.00
R0047:Dctn1	UTSW	6	83,159,614 (GRCm39)	nonsense	probably null
R0047:Dctn1	UTSW	6	83,159,614 (GRCm39)	nonsense	probably null
R0057:Dctn1	UTSW	6	83,156,874 (GRCm39)	missense	probably benign	0.14
R0731:Dctn1	UTSW	6	83,160,071 (GRCm39)	missense	probably damaging	0.98
R0738:Dctn1	UTSW	6	83,167,089 (GRCm39)	critical splice donor site	probably null
R0755:Dctn1	UTSW	6	83,166,059 (GRCm39)	missense	probably damaging	0.96
R0839:Dctn1	UTSW	6	83,167,459 (GRCm39)	missense	possibly damaging	0.53
R1035:Dctn1	UTSW	6	83,167,202 (GRCm39)	missense	probably damaging	1.00
R1454:Dctn1	UTSW	6	83,174,490 (GRCm39)	missense	possibly damaging	0.93
R1469:Dctn1	UTSW	6	83,169,871 (GRCm39)	missense	probably damaging	1.00
R1469:Dctn1	UTSW	6	83,169,871 (GRCm39)	missense	probably damaging	1.00
R1627:Dctn1	UTSW	6	83,172,064 (GRCm39)	missense	probably damaging	0.99
R1631:Dctn1	UTSW	6	83,174,578 (GRCm39)	missense	possibly damaging	0.56
R1812:Dctn1	UTSW	6	83,169,500 (GRCm39)	missense	possibly damaging	0.85
R1928:Dctn1	UTSW	6	83,176,166 (GRCm39)	splice site	probably benign
R2008:Dctn1	UTSW	6	83,166,938 (GRCm39)	missense	probably damaging	0.99
R2242:Dctn1	UTSW	6	83,176,687 (GRCm39)	missense	probably damaging	0.99
R2259:Dctn1	UTSW	6	83,174,568 (GRCm39)	missense	possibly damaging	0.46
R2422:Dctn1	UTSW	6	83,176,782 (GRCm39)	missense	possibly damaging	0.92
R2483:Dctn1	UTSW	6	83,171,169 (GRCm39)	missense	probably damaging	1.00
R4455:Dctn1	UTSW	6	83,172,031 (GRCm39)	missense	probably damaging	1.00
R4724:Dctn1	UTSW	6	83,166,920 (GRCm39)	missense	possibly damaging	0.53
R4819:Dctn1	UTSW	6	83,167,501 (GRCm39)	missense	probably damaging	0.97
R4831:Dctn1	UTSW	6	83,176,753 (GRCm39)	missense	possibly damaging	0.46
R4928:Dctn1	UTSW	6	83,166,189 (GRCm39)	missense	possibly damaging	0.73
R5087:Dctn1	UTSW	6	83,168,621 (GRCm39)	missense	probably damaging	1.00
R5354:Dctn1	UTSW	6	83,160,108 (GRCm39)	missense	possibly damaging	0.93
R5372:Dctn1	UTSW	6	83,167,192 (GRCm39)	missense	probably damaging	0.96
R5493:Dctn1	UTSW	6	83,159,546 (GRCm39)	missense	possibly damaging	0.89
R5494:Dctn1	UTSW	6	83,159,546 (GRCm39)	missense	possibly damaging	0.89
R5732:Dctn1	UTSW	6	83,174,931 (GRCm39)	critical splice donor site	probably null
R5856:Dctn1	UTSW	6	83,174,847 (GRCm39)	missense	probably damaging	1.00
R6025:Dctn1	UTSW	6	83,170,673 (GRCm39)	splice site	probably null
R6999:Dctn1	UTSW	6	83,168,263 (GRCm39)	missense	possibly damaging	0.89
R7052:Dctn1	UTSW	6	83,172,262 (GRCm39)	splice site	probably null
R7133:Dctn1	UTSW	6	83,157,026 (GRCm39)	splice site	probably null
R7485:Dctn1	UTSW	6	83,166,887 (GRCm39)	missense	possibly damaging	0.85
R7607:Dctn1	UTSW	6	83,172,051 (GRCm39)	nonsense	probably null
R7729:Dctn1	UTSW	6	83,160,042 (GRCm39)	missense	probably damaging	1.00
R7749:Dctn1	UTSW	6	83,163,123 (GRCm39)	intron	probably benign
R8282:Dctn1	UTSW	6	83,176,738 (GRCm39)	missense	possibly damaging	0.91
R8750:Dctn1	UTSW	6	83,160,108 (GRCm39)	missense	possibly damaging	0.93
R9126:Dctn1	UTSW	6	83,169,835 (GRCm39)	missense	probably damaging	0.99
R9208:Dctn1	UTSW	6	83,176,684 (GRCm39)	missense	probably benign	0.33
R9422:Dctn1	UTSW	6	83,170,691 (GRCm39)	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- AAGGTGAGCCTCAGGTGAC -3'
(R):5'- GCAACAATGTGTCCACGCC -3'

Sequencing Primer
(F):5'- AGCCTCAGGTGACGCTGC -3'
(R):5'- CTCTTACCTGCCAGCTGG -3'

Posted On

2016-02-04