Mutagenetix > Incidental Mutation

Incidental Mutation 'R4802:Ryr2'

370324

Institutional Source

Beutler Lab

Gene Symbol

Ryr2

Ensembl Gene

ENSMUSG00000021313

Gene Name

ryanodine receptor 2, cardiac

Synonyms

9330127I20Rik

MMRRC Submission

042424-MU

Accession Numbers

Ncbi RefSeq: NM_023868.2; MGI: 99685

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4802 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

11553102-12106945 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 11708227 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 2509 (T2509A)

Ref Sequence

ENSEMBL: ENSMUSP00000127991 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021750] [ENSMUST00000170156]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000021750
AA Change: T2509A

PolyPhen 2 Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000021750
Gene: ENSMUSG00000021313
AA Change: T2509A

Domain	Start	End	E-Value	Type
MIR	110	165	4.19e-2	SMART
MIR	172	217	9.25e-4	SMART
MIR	225	280	1.8e-1	SMART
MIR	286	376	2.22e-24	SMART
Pfam:RYDR_ITPR	454	648	3.1e-65	PFAM
SPRY	670	808	1.56e-30	SMART
Pfam:RyR	862	952	1.8e-36	PFAM
Pfam:RyR	976	1066	1.1e-32	PFAM
SPRY	1098	1221	5.07e-39	SMART
SPRY	1423	1562	7.47e-28	SMART
low complexity region	1643	1653	N/A	INTRINSIC
low complexity region	1872	1891	N/A	INTRINSIC
Pfam:RYDR_ITPR	2122	2331	1.2e-71	PFAM
low complexity region	2372	2379	N/A	INTRINSIC
low complexity region	2416	2426	N/A	INTRINSIC
low complexity region	2497	2510	N/A	INTRINSIC
Pfam:RyR	2700	2790	1.1e-33	PFAM
Pfam:RyR	2820	2904	7.1e-27	PFAM
PDB:2BCX\|B	3580	3609	9e-12	PDB
low complexity region	3700	3720	N/A	INTRINSIC
Pfam:RIH_assoc	3829	3947	3.1e-36	PFAM
EFh	4026	4054	1.36e0	SMART
EFh	4061	4089	5.92e1	SMART
low complexity region	4218	4227	N/A	INTRINSIC
low complexity region	4256	4273	N/A	INTRINSIC
transmembrane domain	4278	4300	N/A	INTRINSIC
low complexity region	4309	4317	N/A	INTRINSIC
Pfam:RR_TM4-6	4332	4598	5.7e-96	PFAM
Pfam:Ion_trans	4710	4877	8e-16	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000170156
AA Change: T2509A

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000127991
Gene: ENSMUSG00000021313
AA Change: T2509A

Domain	Start	End	E-Value	Type
MIR	110	165	4.19e-2	SMART
MIR	172	217	9.25e-4	SMART
MIR	225	280	1.8e-1	SMART
MIR	286	376	2.22e-24	SMART
Pfam:RYDR_ITPR	451	655	3.5e-73	PFAM
SPRY	670	808	1.56e-30	SMART
Pfam:RyR	861	955	1.4e-33	PFAM
Pfam:RyR	975	1069	9.2e-34	PFAM
SPRY	1098	1221	5.07e-39	SMART
SPRY	1423	1562	7.47e-28	SMART
low complexity region	1643	1653	N/A	INTRINSIC
low complexity region	1872	1891	N/A	INTRINSIC
Pfam:RYDR_ITPR	2120	2331	3.9e-65	PFAM
low complexity region	2372	2379	N/A	INTRINSIC
low complexity region	2416	2426	N/A	INTRINSIC
low complexity region	2497	2510	N/A	INTRINSIC
Pfam:RyR	2699	2793	1.1e-37	PFAM
Pfam:RyR	2819	2907	9.4e-34	PFAM
PDB:2BCX\|B	3580	3609	9e-12	PDB
low complexity region	3700	3720	N/A	INTRINSIC
Pfam:RIH_assoc	3825	3958	2.3e-42	PFAM
EFh	4026	4054	1.36e0	SMART
EFh	4061	4089	5.92e1	SMART
low complexity region	4218	4227	N/A	INTRINSIC
low complexity region	4256	4273	N/A	INTRINSIC
transmembrane domain	4278	4300	N/A	INTRINSIC
low complexity region	4309	4317	N/A	INTRINSIC
Pfam:RR_TM4-6	4332	4598	5.1e-93	PFAM
Pfam:Ion_trans	4705	4865	9.3e-11	PFAM

Meta Mutation Damage Score

0.2267

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

97% (93/96)

MGI Phenotype

Strain: 3640298
Lethality: E9-E11
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice show embryonic lethality during organogenesis and altered cardiomyocyte morphology. Homozygotes for a phosphorylation defective allele show decreased susceptibility to myocardial infarction-induced heart failure. Homozygotes for the R420W allele show lymphoid organ hypertrophy. [provided by MGI curators]

Allele List at MGI

All alleles(44) : Targeted(17) Gene trapped(27)

Other mutations in this stock

Total: 166 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	A	G	10: 28,983,926 (GRCm38)		probably null	Het
4932416K20Rik	T	A	8: 104,797,032 (GRCm38)		noncoding transcript	Het
Aadacl3	A	G	4: 144,456,232 (GRCm38)	I222T	probably damaging	Het
Abca13	G	T	11: 9,522,341 (GRCm38)	G4249V	possibly damaging	Het
Abcb10	A	G	8: 123,966,527 (GRCm38)	V346A	probably benign	Het
Abcc2	A	T	19: 43,819,361 (GRCm38)	I814F	probably damaging	Het
AF366264	T	A	8: 13,836,970 (GRCm38)	I374F	possibly damaging	Het
Alkbh2	C	T	5: 114,124,226 (GRCm38)	E148K	probably damaging	Het
Ankrd28	A	C	14: 31,736,830 (GRCm38)	D335E	probably damaging	Het
Ankrd44	T	C	1: 54,762,316 (GRCm38)	H284R	probably damaging	Het
Arfgef3	G	T	10: 18,591,906 (GRCm38)	Q1849K	probably benign	Het
Bach1	T	A	16: 87,722,452 (GRCm38)	D543E	probably damaging	Het
Bahcc1	G	A	11: 120,282,225 (GRCm38)	V1558I	probably benign	Het
Bbs5	T	A	2: 69,655,614 (GRCm38)	W168R	probably damaging	Het
Bcar3	A	T	3: 122,529,594 (GRCm38)	D766V	probably benign	Het
C1ra	A	G	6: 124,513,768 (GRCm38)	D40G	probably benign	Het
Cbr4	T	C	8: 61,490,079 (GRCm38)		probably benign	Het
Ccdc138	G	A	10: 58,573,643 (GRCm38)	C598Y	probably damaging	Het
Cd200r3	T	A	16: 44,957,825 (GRCm38)	N197K	possibly damaging	Het
Cenpf	T	A	1: 189,651,220 (GRCm38)	E2634D	probably damaging	Het
Cisd2	T	C	3: 135,411,141 (GRCm38)	K63R	probably damaging	Het
Clca3a2	A	T	3: 144,807,351 (GRCm38)	S478T	possibly damaging	Het
Clptm1l	T	C	13: 73,607,862 (GRCm38)	M199T	possibly damaging	Het
Cntnap4	T	A	8: 112,773,590 (GRCm38)	S505T	possibly damaging	Het
Cr2	C	T	1: 195,163,311 (GRCm38)	G112D	probably damaging	Het
Crb2	T	A	2: 37,793,756 (GRCm38)	I1090N	probably benign	Het
Csmd3	G	T	15: 47,621,292 (GRCm38)	P3057Q	probably damaging	Het
Ctso	G	A	3: 81,954,240 (GRCm38)	V307I	probably damaging	Het
Cyp3a41b	T	A	5: 145,573,651 (GRCm38)	T138S	probably benign	Het
Dctn3	G	T	4: 41,719,904 (GRCm38)	Y67*	probably null	Het
Dennd4c	C	A	4: 86,819,884 (GRCm38)	Y918*	probably null	Het
Dlg5	C	T	14: 24,154,689 (GRCm38)	G1262D	probably damaging	Het
Dnaaf1	G	A	8: 119,577,361 (GRCm38)	G46D	probably benign	Het
Dnah6	A	G	6: 73,089,698 (GRCm38)	V2563A	probably damaging	Het
Dnajc13	A	G	9: 104,175,727 (GRCm38)	Y1679H	probably benign	Het
Eif2ak3	T	C	6: 70,887,893 (GRCm38)	Y578H	probably benign	Het
Endod1	C	T	9: 14,357,023 (GRCm38)	V389M	probably benign	Het
Entpd2	T	G	2: 25,399,764 (GRCm38)		probably null	Het
Ephb4	T	C	5: 137,365,506 (GRCm38)	L582P	probably damaging	Het
Eps15	T	C	4: 109,324,217 (GRCm38)	L316S	possibly damaging	Het
Erbb4	G	T	1: 68,330,246 (GRCm38)	T412K	probably damaging	Het
Fam117a	T	A	11: 95,364,070 (GRCm38)	F90I	probably damaging	Het
Fam187b	T	G	7: 30,977,090 (GRCm38)	V8G	possibly damaging	Het
Fam84b	C	A	15: 60,823,944 (GRCm38)		probably benign	Het
Far1	T	A	7: 113,539,453 (GRCm38)	I59N	possibly damaging	Het
Fbxo34	T	A	14: 47,530,869 (GRCm38)	L562Q	probably damaging	Het
Frem1	T	A	4: 82,916,628 (GRCm38)		probably benign	Het
Gfra3	G	T	18: 34,720,192 (GRCm38)	P10Q	probably damaging	Het
Gm10619	T	A	7: 73,810,025 (GRCm38)		noncoding transcript	Het
Gm11487	C	T	4: 73,401,267 (GRCm38)	W80*	probably null	Het
Gm19965	T	A	1: 116,821,896 (GRCm38)	Y436N	probably benign	Het
Gm21818	T	A	13: 120,173,222 (GRCm38)	S13R	probably benign	Het
Gm28042	T	C	2: 120,042,054 (GRCm38)		probably benign	Het
Gm4953	A	T	1: 159,168,418 (GRCm38)		noncoding transcript	Het
Gm5799	A	T	14: 43,544,548 (GRCm38)	H59L	probably damaging	Het
Gmppb	T	A	9: 108,050,217 (GRCm38)	V121E	probably benign	Het
Ighv7-4	G	C	12: 114,223,279 (GRCm38)		probably benign	Het
Ipo4	A	G	14: 55,631,214 (GRCm38)	S446P	probably damaging	Het
Itpr2	T	C	6: 146,371,331 (GRCm38)	T855A	probably damaging	Het
Jaml	T	C	9: 45,101,064 (GRCm38)	I283T	possibly damaging	Het
Klhl32	T	C	4: 24,649,698 (GRCm38)	Y399C	possibly damaging	Het
Lrriq1	G	A	10: 103,221,318 (GRCm38)	T207I	probably benign	Het
Lrriq3	A	T	3: 155,187,970 (GRCm38)	H436L	probably benign	Het
Mad2l1bp	T	C	17: 46,148,263 (GRCm38)	K114E	possibly damaging	Het
Mamstr	T	C	7: 45,642,418 (GRCm38)	V64A	possibly damaging	Het
Map4	T	A	9: 110,035,257 (GRCm38)	S517T	probably benign	Het
Matn1	A	T	4: 130,950,025 (GRCm38)	I182F	possibly damaging	Het
Mcm3	A	G	1: 20,810,156 (GRCm38)	I484T	probably damaging	Het
Med13	T	A	11: 86,278,773 (GRCm38)	I1922F	probably damaging	Het
Metap2	A	G	10: 93,868,895 (GRCm38)	V137A	probably damaging	Het
Mex3d	A	G	10: 80,386,954 (GRCm38)	V156A	possibly damaging	Het
Mfsd6	G	A	1: 52,709,596 (GRCm38)	P37S	probably benign	Het
Mkl1	T	C	15: 81,104,799 (GRCm38)	E7G	probably benign	Het
Mrgprx1	A	C	7: 48,021,211 (GRCm38)	S263A	possibly damaging	Het
Msl1	C	T	11: 98,803,969 (GRCm38)	R505*	probably null	Het
Mta2	T	C	19: 8,945,851 (GRCm38)	S96P	probably damaging	Het
Mtr	A	T	13: 12,195,251 (GRCm38)	N986K	probably benign	Het
Mut	A	G	17: 40,937,351 (GRCm38)	T90A	probably benign	Het
Mutyh	C	T	4: 116,817,029 (GRCm38)	T259I	probably benign	Het
Myof	T	C	19: 37,945,738 (GRCm38)	T908A	probably benign	Het
Nav2	A	T	7: 49,545,852 (GRCm38)	D992V	possibly damaging	Het
Neb	T	C	2: 52,200,703 (GRCm38)	T1352A	possibly damaging	Het
Nfix	CAAAAA	CAAAA	8: 84,716,247 (GRCm38)		probably null	Het
Nudt19	T	C	7: 35,556,139 (GRCm38)		probably benign	Het
Nudt6	G	A	3: 37,405,354 (GRCm38)	R161C	probably benign	Het
Olfr1122	T	A	2: 87,388,209 (GRCm38)	V168E	probably benign	Het
Olfr1173	T	A	2: 88,274,879 (GRCm38)	M57L	probably damaging	Het
Olfr125	T	C	17: 37,835,349 (GRCm38)	Y117H	probably damaging	Het
Olfr1276	T	A	2: 111,257,152 (GRCm38)	F12L	probably damaging	Het
Olfr1411	T	A	1: 92,596,998 (GRCm38)	C160S	probably benign	Het
Olfr154	T	A	2: 85,664,278 (GRCm38)	D52V	probably damaging	Het
Olfr23	T	A	11: 73,940,870 (GRCm38)	I208K	possibly damaging	Het
Olfr319	T	C	11: 58,701,791 (GRCm38)	V30A	probably benign	Het
Olfr456	A	T	6: 42,486,679 (GRCm38)	N171K	probably benign	Het
Olfr744	A	G	14: 50,619,022 (GRCm38)	T267A	probably benign	Het
Olfr959	T	G	9: 39,572,858 (GRCm38)	M134L	probably benign	Het
Olr1	A	G	6: 129,488,090 (GRCm38)	F141S	possibly damaging	Het
Oprl1	G	T	2: 181,719,253 (GRCm38)	M340I	probably benign	Het
Otogl	A	C	10: 107,901,336 (GRCm38)	C72W	probably damaging	Het
Pcdha11	T	A	18: 37,005,465 (GRCm38)	I49N	probably damaging	Het
Pcdha4	T	A	18: 36,953,955 (GRCm38)	L397*	probably null	Het
Pcdhb14	A	G	18: 37,448,278 (GRCm38)	S146G	probably benign	Het
Pclo	T	A	5: 14,675,815 (GRCm38)	H1562Q	unknown	Het
Pcsk9	T	C	4: 106,447,569 (GRCm38)	E434G	probably benign	Het
Phc2	A	G	4: 128,751,598 (GRCm38)	K833E	probably damaging	Het
Pja2	A	T	17: 64,292,862 (GRCm38)	S480R	probably damaging	Het
Pkd1	G	A	17: 24,578,096 (GRCm38)	G2493D	probably damaging	Het
Plk5	G	A	10: 80,359,304 (GRCm38)	V179M	possibly damaging	Het
Polr1a	G	A	6: 71,976,070 (GRCm38)	V1541I	probably benign	Het
Ppard	C	G	17: 28,286,374 (GRCm38)	R12G	unknown	Het
Ppp1r14a	A	G	7: 29,291,526 (GRCm38)	D73G	probably damaging	Het
Psd3	C	T	8: 68,121,148 (GRCm38)	R127H	probably benign	Het
Pten	G	T	19: 32,758,503 (GRCm38)	G20V	possibly damaging	Het
Ptprf	G	A	4: 118,210,329 (GRCm38)		probably benign	Het
Rad54l	T	C	4: 116,122,924 (GRCm38)	D21G	probably null	Het
Rgs14	T	C	13: 55,380,957 (GRCm38)	Y304H	probably damaging	Het
Rgs9	T	C	11: 109,240,868 (GRCm38)	K346R	probably damaging	Het
Rnf169	C	G	7: 99,926,446 (GRCm38)	G314A	probably damaging	Het
Rpgrip1l	T	A	8: 91,270,177 (GRCm38)	T692S	probably damaging	Het
Rtf1	T	C	2: 119,675,228 (GRCm38)	V54A	possibly damaging	Het
Rtn4	T	C	11: 29,708,660 (GRCm38)	V938A	probably benign	Het
Scel	A	C	14: 103,583,100 (GRCm38)	T348P	probably benign	Het
Scgb1b2	G	T	7: 31,291,573 (GRCm38)	L37I	possibly damaging	Het
Sdf4	A	G	4: 156,000,721 (GRCm38)	H171R	possibly damaging	Het
Sec31a	A	G	5: 100,393,363 (GRCm38)	V295A	probably damaging	Het
Setx	T	A	2: 29,146,373 (GRCm38)	S957T	probably benign	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Six5	A	G	7: 19,096,969 (GRCm38)	N507S	probably benign	Het
Slc12a7	T	C	13: 73,763,892 (GRCm38)		probably null	Het
Slc1a7	T	A	4: 107,993,040 (GRCm38)	V116E	probably damaging	Het
Slc22a1	A	G	17: 12,675,535 (GRCm38)	L42P	probably damaging	Het
Slc25a31	A	T	3: 40,721,545 (GRCm38)	I174F	probably damaging	Het
Slc31a2	A	T	4: 62,292,632 (GRCm38)	M3L	probably damaging	Het
Slco1a4	T	A	6: 141,845,497 (GRCm38)		probably benign	Het
Smcr8	C	T	11: 60,778,610 (GRCm38)		probably null	Het
Smg5	G	T	3: 88,355,692 (GRCm38)	E801*	probably null	Het
Smgc	C	A	15: 91,854,616 (GRCm38)	H492Q	probably benign	Het
Smyd4	G	T	11: 75,403,184 (GRCm38)	G694V	probably damaging	Het
Sorcs3	A	G	19: 48,398,744 (GRCm38)	T223A	possibly damaging	Het
Stab1	G	T	14: 31,141,371 (GRCm38)	C2119*	probably null	Het
Taar9	A	G	10: 24,108,843 (GRCm38)	I231T	probably damaging	Het
Tacr2	A	G	10: 62,261,548 (GRCm38)	Y269C	probably damaging	Het
Taf3	T	G	2: 9,951,123 (GRCm38)	K744N	possibly damaging	Het
Tarbp1	C	G	8: 126,474,889 (GRCm38)	E59D	possibly damaging	Het
Tenm4	T	A	7: 96,906,245 (GRCm38)	V2682E	probably damaging	Het
Tet1	A	C	10: 62,822,663 (GRCm38)	L1468R	probably damaging	Het
Tgfb2	A	T	1: 186,628,913 (GRCm38)	Y380*	probably null	Het
Tgm5	T	G	2: 121,052,472 (GRCm38)	K435Q	probably damaging	Het
Themis	A	G	10: 28,761,511 (GRCm38)	T204A	probably benign	Het
Tm4sf1	T	C	3: 57,294,679 (GRCm38)	Y37C	probably damaging	Het
Tnn	T	C	1: 160,145,033 (GRCm38)	N333S	possibly damaging	Het
Tppp2	A	G	14: 51,919,348 (GRCm38)	N61D	probably benign	Het
Treml2	A	G	17: 48,309,159 (GRCm38)	T276A	probably benign	Het
Trit1	T	C	4: 123,016,638 (GRCm38)	V10A	probably benign	Het
Ttn	T	A	2: 76,964,646 (GRCm38)		probably benign	Het
Uba1y	T	G	Y: 825,890 (GRCm38)		probably null	Het
Vcam1	C	G	3: 116,115,935 (GRCm38)	G581A	probably damaging	Het
Vmn1r16	G	A	6: 57,323,190 (GRCm38)	T149I	probably benign	Het
Vmn1r209	T	C	13: 22,805,656 (GRCm38)	D288G	probably damaging	Het
Vmn1r78	T	A	7: 12,152,964 (GRCm38)	Y167*	probably null	Het
Vmn2r103	T	A	17: 19,795,076 (GRCm38)	S493T	probably benign	Het
Vmn2r105	T	A	17: 20,227,294 (GRCm38)	M423L	probably benign	Het
Vps13c	T	C	9: 67,964,282 (GRCm38)	F3244L	probably damaging	Het
Zfp119b	A	T	17: 55,939,642 (GRCm38)	D149E	probably damaging	Het
Zfp345	T	C	2: 150,473,308 (GRCm38)	Y103C	possibly damaging	Het
Zmym6	C	T	4: 127,123,216 (GRCm38)	T930I	probably benign	Het

Other mutations in Ryr2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00518:Ryr2	APN	13	11,834,092 (GRCm38)	splice site	probably benign
IGL00757:Ryr2	APN	13	11,618,604 (GRCm38)	splice site	probably null
IGL00838:Ryr2	APN	13	11,568,503 (GRCm38)	missense	probably damaging	0.98
IGL00849:Ryr2	APN	13	11,585,478 (GRCm38)	missense	possibly damaging	0.91
IGL00987:Ryr2	APN	13	11,735,502 (GRCm38)	missense	probably damaging	0.99
IGL01096:Ryr2	APN	13	11,703,544 (GRCm38)	missense	probably damaging	1.00
IGL01313:Ryr2	APN	13	11,638,485 (GRCm38)	critical splice acceptor site	probably null
IGL01349:Ryr2	APN	13	11,587,239 (GRCm38)	missense	possibly damaging	0.93
IGL01391:Ryr2	APN	13	11,556,685 (GRCm38)	missense	possibly damaging	0.96
IGL01401:Ryr2	APN	13	11,591,352 (GRCm38)	missense	possibly damaging	0.80
IGL01412:Ryr2	APN	13	11,742,036 (GRCm38)	missense	probably benign	0.10
IGL01419:Ryr2	APN	13	11,799,837 (GRCm38)	missense	possibly damaging	0.51
IGL01432:Ryr2	APN	13	11,851,204 (GRCm38)	missense	possibly damaging	0.63
IGL01533:Ryr2	APN	13	11,721,790 (GRCm38)	missense	probably damaging	1.00
IGL01571:Ryr2	APN	13	11,721,761 (GRCm38)	missense	probably damaging	1.00
IGL01584:Ryr2	APN	13	11,601,758 (GRCm38)	critical splice donor site	probably null
IGL01611:Ryr2	APN	13	11,591,316 (GRCm38)	missense	possibly damaging	0.67
IGL01632:Ryr2	APN	13	11,594,968 (GRCm38)	missense	probably damaging	0.97
IGL01643:Ryr2	APN	13	11,692,677 (GRCm38)	missense	possibly damaging	0.94
IGL01647:Ryr2	APN	13	11,585,480 (GRCm38)	missense	probably damaging	1.00
IGL01730:Ryr2	APN	13	11,601,842 (GRCm38)	missense	possibly damaging	0.86
IGL01834:Ryr2	APN	13	11,595,425 (GRCm38)	missense	possibly damaging	0.71
IGL01921:Ryr2	APN	13	11,554,550 (GRCm38)	missense	possibly damaging	0.96
IGL01937:Ryr2	APN	13	11,790,363 (GRCm38)	missense	probably damaging	1.00
IGL01945:Ryr2	APN	13	11,790,363 (GRCm38)	missense	probably damaging	1.00
IGL02027:Ryr2	APN	13	11,597,112 (GRCm38)	missense	probably damaging	1.00
IGL02060:Ryr2	APN	13	11,747,564 (GRCm38)	missense	probably damaging	1.00
IGL02065:Ryr2	APN	13	11,572,257 (GRCm38)	missense	possibly damaging	0.92
IGL02084:Ryr2	APN	13	11,792,762 (GRCm38)	nonsense	probably null
IGL02086:Ryr2	APN	13	11,735,556 (GRCm38)	missense	probably damaging	1.00
IGL02095:Ryr2	APN	13	11,759,759 (GRCm38)	missense	probably damaging	0.98
IGL02100:Ryr2	APN	13	11,737,873 (GRCm38)	missense	possibly damaging	0.92
IGL02122:Ryr2	APN	13	11,741,869 (GRCm38)	missense	probably damaging	1.00
IGL02202:Ryr2	APN	13	11,730,388 (GRCm38)	missense	probably damaging	0.97
IGL02202:Ryr2	APN	13	11,747,658 (GRCm38)	splice site	probably benign
IGL02369:Ryr2	APN	13	11,619,496 (GRCm38)	missense	possibly damaging	0.68
IGL02383:Ryr2	APN	13	11,722,721 (GRCm38)	splice site	probably benign
IGL02400:Ryr2	APN	13	11,605,244 (GRCm38)	splice site	probably benign
IGL02423:Ryr2	APN	13	11,745,198 (GRCm38)	missense	probably damaging	1.00
IGL02425:Ryr2	APN	13	11,745,674 (GRCm38)	missense	probably damaging	0.99
IGL02458:Ryr2	APN	13	11,705,699 (GRCm38)	missense	probably benign	0.15
IGL02602:Ryr2	APN	13	11,554,511 (GRCm38)	utr 3 prime	probably benign
IGL02694:Ryr2	APN	13	11,605,189 (GRCm38)	missense	probably damaging	1.00
IGL02726:Ryr2	APN	13	11,738,320 (GRCm38)	missense	probably damaging	1.00
IGL02747:Ryr2	APN	13	11,655,677 (GRCm38)	missense	probably damaging	1.00
IGL02795:Ryr2	APN	13	11,595,190 (GRCm38)	missense	probably benign	0.21
IGL02876:Ryr2	APN	13	11,707,793 (GRCm38)	missense	probably benign	0.39
IGL02878:Ryr2	APN	13	11,918,319 (GRCm38)	missense	probably benign	0.10
IGL02887:Ryr2	APN	13	11,591,269 (GRCm38)	missense	probably damaging	0.97
IGL02926:Ryr2	APN	13	11,759,835 (GRCm38)	missense	probably damaging	0.99
IGL03030:Ryr2	APN	13	11,684,479 (GRCm38)	missense	probably damaging	0.99
IGL03064:Ryr2	APN	13	11,643,902 (GRCm38)	critical splice acceptor site	probably null
IGL03102:Ryr2	APN	13	11,635,582 (GRCm38)	splice site	probably benign
IGL03152:Ryr2	APN	13	11,853,150 (GRCm38)	missense	probably damaging	1.00
IGL03176:Ryr2	APN	13	11,742,023 (GRCm38)	nonsense	probably null
IGL03180:Ryr2	APN	13	11,568,563 (GRCm38)	missense	possibly damaging	0.95
IGL03213:Ryr2	APN	13	11,724,387 (GRCm38)	splice site	probably benign
IGL03390:Ryr2	APN	13	11,772,416 (GRCm38)	missense	probably benign
IGL03410:Ryr2	APN	13	11,588,147 (GRCm38)	missense	probably damaging	0.99
Arruda	UTSW	13	11,643,895 (GRCm38)	missense	probably damaging	1.00
Arruda2	UTSW	13	11,879,496 (GRCm38)	missense	probably damaging	1.00
Arruda3	UTSW	13	11,555,448 (GRCm38)	missense	possibly damaging	0.91
barricuda	UTSW	13	11,595,014 (GRCm38)	missense	probably benign	0.06
BB006:Ryr2	UTSW	13	11,690,295 (GRCm38)	nonsense	probably null
BB006:Ryr2	UTSW	13	11,594,794 (GRCm38)	missense	probably damaging	1.00
BB016:Ryr2	UTSW	13	11,690,295 (GRCm38)	nonsense	probably null
BB016:Ryr2	UTSW	13	11,594,794 (GRCm38)	missense	probably damaging	1.00
H8562:Ryr2	UTSW	13	11,717,141 (GRCm38)	splice site	probably benign
IGL02799:Ryr2	UTSW	13	11,665,962 (GRCm38)	missense	probably damaging	1.00
IGL02991:Ryr2	UTSW	13	11,761,306 (GRCm38)	missense	probably damaging	0.99
PIT4142001:Ryr2	UTSW	13	11,707,796 (GRCm38)	missense	probably damaging	0.97
PIT4260001:Ryr2	UTSW	13	11,594,755 (GRCm38)	missense	possibly damaging	0.93
PIT4458001:Ryr2	UTSW	13	11,555,448 (GRCm38)	missense	probably benign	0.29
R0003:Ryr2	UTSW	13	11,824,379 (GRCm38)	missense	probably damaging	1.00
R0004:Ryr2	UTSW	13	11,665,919 (GRCm38)	missense	probably benign
R0018:Ryr2	UTSW	13	11,595,223 (GRCm38)	missense	possibly damaging	0.94
R0048:Ryr2	UTSW	13	11,595,784 (GRCm38)	missense	probably damaging	1.00
R0048:Ryr2	UTSW	13	11,595,784 (GRCm38)	missense	probably damaging	1.00
R0056:Ryr2	UTSW	13	11,669,038 (GRCm38)	missense	probably damaging	0.97
R0062:Ryr2	UTSW	13	11,869,116 (GRCm38)	critical splice donor site	probably null
R0062:Ryr2	UTSW	13	11,869,116 (GRCm38)	critical splice donor site	probably null
R0080:Ryr2	UTSW	13	11,568,475 (GRCm38)	missense	probably damaging	0.98
R0116:Ryr2	UTSW	13	11,709,921 (GRCm38)	missense	probably damaging	1.00
R0148:Ryr2	UTSW	13	11,714,548 (GRCm38)	missense	probably damaging	1.00
R0206:Ryr2	UTSW	13	11,676,251 (GRCm38)	splice site	probably benign
R0226:Ryr2	UTSW	13	11,772,556 (GRCm38)	missense	probably damaging	1.00
R0285:Ryr2	UTSW	13	11,716,977 (GRCm38)	missense	probably damaging	1.00
R0365:Ryr2	UTSW	13	11,668,839 (GRCm38)	missense	possibly damaging	0.90
R0401:Ryr2	UTSW	13	11,705,684 (GRCm38)	missense	probably benign	0.45
R0415:Ryr2	UTSW	13	11,869,156 (GRCm38)	missense	probably damaging	0.97
R0418:Ryr2	UTSW	13	11,834,095 (GRCm38)	splice site	probably benign
R0558:Ryr2	UTSW	13	11,799,861 (GRCm38)	missense	probably damaging	1.00
R0558:Ryr2	UTSW	13	11,638,443 (GRCm38)	missense	probably damaging	1.00
R0574:Ryr2	UTSW	13	11,731,669 (GRCm38)	missense	probably benign	0.02
R0586:Ryr2	UTSW	13	11,635,559 (GRCm38)	missense	probably null
R0601:Ryr2	UTSW	13	11,705,633 (GRCm38)	critical splice donor site	probably null
R0610:Ryr2	UTSW	13	11,622,952 (GRCm38)	missense	probably damaging	1.00
R0648:Ryr2	UTSW	13	11,724,333 (GRCm38)	missense	possibly damaging	0.86
R0727:Ryr2	UTSW	13	11,566,885 (GRCm38)	missense	probably damaging	1.00
R0743:Ryr2	UTSW	13	11,554,529 (GRCm38)	missense	probably damaging	0.99
R0821:Ryr2	UTSW	13	11,738,126 (GRCm38)	missense	probably benign	0.35
R0884:Ryr2	UTSW	13	11,554,529 (GRCm38)	missense	probably damaging	0.99
R1104:Ryr2	UTSW	13	11,669,969 (GRCm38)	missense	probably damaging	0.99
R1114:Ryr2	UTSW	13	11,945,981 (GRCm38)	missense	probably damaging	0.98
R1167:Ryr2	UTSW	13	11,660,113 (GRCm38)	missense	possibly damaging	0.94
R1238:Ryr2	UTSW	13	11,759,703 (GRCm38)	missense	probably damaging	1.00
R1239:Ryr2	UTSW	13	11,883,043 (GRCm38)	critical splice donor site	probably null
R1296:Ryr2	UTSW	13	11,687,879 (GRCm38)	splice site	probably benign
R1400:Ryr2	UTSW	13	11,595,076 (GRCm38)	missense	probably benign	0.08
R1439:Ryr2	UTSW	13	11,714,503 (GRCm38)	splice site	probably benign
R1443:Ryr2	UTSW	13	11,779,266 (GRCm38)	missense	probably benign	0.19
R1446:Ryr2	UTSW	13	11,738,149 (GRCm38)	missense	probably benign	0.09
R1458:Ryr2	UTSW	13	11,727,022 (GRCm38)	missense	probably damaging	0.97
R1497:Ryr2	UTSW	13	11,601,841 (GRCm38)	missense	probably damaging	0.99
R1505:Ryr2	UTSW	13	11,554,592 (GRCm38)	missense	possibly damaging	0.84
R1548:Ryr2	UTSW	13	11,554,549 (GRCm38)	nonsense	probably null
R1551:Ryr2	UTSW	13	11,785,143 (GRCm38)	critical splice acceptor site	probably null
R1567:Ryr2	UTSW	13	11,759,677 (GRCm38)	missense	possibly damaging	0.87
R1581:Ryr2	UTSW	13	11,794,563 (GRCm38)	missense	probably benign	0.01
R1645:Ryr2	UTSW	13	11,718,482 (GRCm38)	nonsense	probably null
R1686:Ryr2	UTSW	13	11,603,779 (GRCm38)	splice site	probably benign
R1696:Ryr2	UTSW	13	11,731,657 (GRCm38)	missense	probably benign	0.02
R1708:Ryr2	UTSW	13	11,587,442 (GRCm38)	splice site	probably null
R1728:Ryr2	UTSW	13	11,587,422 (GRCm38)	missense	possibly damaging	0.94
R1745:Ryr2	UTSW	13	11,790,267 (GRCm38)	missense	probably damaging	1.00
R1771:Ryr2	UTSW	13	11,745,176 (GRCm38)	critical splice donor site	probably null
R1776:Ryr2	UTSW	13	11,745,176 (GRCm38)	critical splice donor site	probably null
R1783:Ryr2	UTSW	13	11,700,371 (GRCm38)	nonsense	probably null
R1801:Ryr2	UTSW	13	11,595,281 (GRCm38)	missense	probably benign	0.01
R1812:Ryr2	UTSW	13	11,560,586 (GRCm38)	missense	probably damaging	0.97
R1820:Ryr2	UTSW	13	11,587,316 (GRCm38)	missense	probably damaging	0.99
R1835:Ryr2	UTSW	13	11,769,878 (GRCm38)	missense	probably benign	0.06
R1868:Ryr2	UTSW	13	11,731,700 (GRCm38)	missense	probably benign	0.02
R1869:Ryr2	UTSW	13	11,662,075 (GRCm38)	missense	probably damaging	0.98
R1884:Ryr2	UTSW	13	11,738,356 (GRCm38)	missense	probably damaging	0.97
R1892:Ryr2	UTSW	13	11,658,958 (GRCm38)	nonsense	probably null
R1897:Ryr2	UTSW	13	11,750,932 (GRCm38)	missense	probably benign	0.09
R1899:Ryr2	UTSW	13	11,591,336 (GRCm38)	missense	probably benign
R1909:Ryr2	UTSW	13	11,700,349 (GRCm38)	missense	probably damaging	1.00
R1918:Ryr2	UTSW	13	11,556,698 (GRCm38)	missense	possibly damaging	0.91
R1937:Ryr2	UTSW	13	11,668,962 (GRCm38)	missense	probably damaging	1.00
R1943:Ryr2	UTSW	13	11,731,723 (GRCm38)	missense	probably benign	0.10
R1956:Ryr2	UTSW	13	11,681,080 (GRCm38)	missense	probably damaging	1.00
R1983:Ryr2	UTSW	13	11,585,402 (GRCm38)	splice site	probably null
R2018:Ryr2	UTSW	13	11,851,188 (GRCm38)	missense	possibly damaging	0.59
R2019:Ryr2	UTSW	13	11,851,188 (GRCm38)	missense	possibly damaging	0.59
R2060:Ryr2	UTSW	13	11,595,736 (GRCm38)	missense	probably damaging	1.00
R2061:Ryr2	UTSW	13	11,665,878 (GRCm38)	splice site	probably null
R2088:Ryr2	UTSW	13	11,662,229 (GRCm38)	missense	probably benign	0.04
R2089:Ryr2	UTSW	13	11,945,977 (GRCm38)	missense	probably benign	0.23
R2091:Ryr2	UTSW	13	11,945,977 (GRCm38)	missense	probably benign	0.23
R2091:Ryr2	UTSW	13	11,945,977 (GRCm38)	missense	probably benign	0.23
R2127:Ryr2	UTSW	13	11,712,195 (GRCm38)	missense	probably damaging	1.00
R2140:Ryr2	UTSW	13	11,560,607 (GRCm38)	missense	probably damaging	1.00
R2153:Ryr2	UTSW	13	11,577,873 (GRCm38)	missense	possibly damaging	0.86
R2179:Ryr2	UTSW	13	11,705,793 (GRCm38)	nonsense	probably null
R2207:Ryr2	UTSW	13	11,810,937 (GRCm38)	missense	probably damaging	1.00
R2237:Ryr2	UTSW	13	11,662,260 (GRCm38)	missense	probably benign	0.18
R2258:Ryr2	UTSW	13	11,738,216 (GRCm38)	missense	possibly damaging	0.94
R2312:Ryr2	UTSW	13	11,738,242 (GRCm38)	missense	probably damaging	1.00
R2421:Ryr2	UTSW	13	11,591,237 (GRCm38)	missense	probably damaging	0.98
R2438:Ryr2	UTSW	13	11,801,848 (GRCm38)	missense	probably damaging	1.00
R2483:Ryr2	UTSW	13	11,759,703 (GRCm38)	missense	probably damaging	1.00
R2860:Ryr2	UTSW	13	11,593,093 (GRCm38)	missense	probably damaging	0.98
R2861:Ryr2	UTSW	13	11,593,093 (GRCm38)	missense	probably damaging	0.98
R2867:Ryr2	UTSW	13	11,761,349 (GRCm38)	missense	probably damaging	1.00
R2867:Ryr2	UTSW	13	11,761,349 (GRCm38)	missense	probably damaging	1.00
R3618:Ryr2	UTSW	13	11,772,580 (GRCm38)	critical splice acceptor site	probably null
R3876:Ryr2	UTSW	13	11,588,159 (GRCm38)	missense	probably damaging	0.99
R3906:Ryr2	UTSW	13	11,738,209 (GRCm38)	missense	possibly damaging	0.87
R3912:Ryr2	UTSW	13	11,772,427 (GRCm38)	missense	probably damaging	0.99
R4018:Ryr2	UTSW	13	11,918,414 (GRCm38)	missense	probably damaging	1.00
R4114:Ryr2	UTSW	13	11,692,682 (GRCm38)	missense	probably damaging	1.00
R4119:Ryr2	UTSW	13	11,779,267 (GRCm38)	missense	probably benign	0.22
R4127:Ryr2	UTSW	13	11,587,437 (GRCm38)	missense	possibly damaging	0.91
R4222:Ryr2	UTSW	13	11,737,873 (GRCm38)	missense	possibly damaging	0.92
R4233:Ryr2	UTSW	13	11,750,725 (GRCm38)	missense	probably benign	0.20
R4355:Ryr2	UTSW	13	11,649,812 (GRCm38)	missense	probably benign	0.05
R4384:Ryr2	UTSW	13	11,605,233 (GRCm38)	missense	probably damaging	0.99
R4422:Ryr2	UTSW	13	11,717,066 (GRCm38)	nonsense	probably null
R4430:Ryr2	UTSW	13	11,735,527 (GRCm38)	missense	probably damaging	0.98
R4624:Ryr2	UTSW	13	12,106,415 (GRCm38)	missense	possibly damaging	0.47
R4663:Ryr2	UTSW	13	11,749,509 (GRCm38)	missense	possibly damaging	0.47
R4665:Ryr2	UTSW	13	11,750,685 (GRCm38)	splice site	probably null
R4668:Ryr2	UTSW	13	11,593,117 (GRCm38)	missense	probably benign
R4677:Ryr2	UTSW	13	11,706,667 (GRCm38)	missense	probably damaging	0.98
R4679:Ryr2	UTSW	13	11,824,369 (GRCm38)	missense	probably benign	0.34
R4680:Ryr2	UTSW	13	11,595,233 (GRCm38)	missense	probably benign	0.04
R4685:Ryr2	UTSW	13	11,692,646 (GRCm38)	missense	probably damaging	1.00
R4709:Ryr2	UTSW	13	11,716,998 (GRCm38)	missense	probably damaging	1.00
R4731:Ryr2	UTSW	13	11,577,909 (GRCm38)	missense	possibly damaging	0.53
R4732:Ryr2	UTSW	13	11,577,909 (GRCm38)	missense	possibly damaging	0.53
R4733:Ryr2	UTSW	13	11,577,909 (GRCm38)	missense	possibly damaging	0.53
R4734:Ryr2	UTSW	13	11,737,753 (GRCm38)	missense	probably damaging	0.99
R4740:Ryr2	UTSW	13	11,657,047 (GRCm38)	missense	possibly damaging	0.95
R4801:Ryr2	UTSW	13	11,708,227 (GRCm38)	missense	probably damaging	1.00
R4801:Ryr2	UTSW	13	11,687,932 (GRCm38)	missense	probably damaging	1.00
R4802:Ryr2	UTSW	13	11,687,932 (GRCm38)	missense	probably damaging	1.00
R4804:Ryr2	UTSW	13	11,717,097 (GRCm38)	missense	probably damaging	1.00
R4811:Ryr2	UTSW	13	11,655,698 (GRCm38)	missense	probably damaging	0.97
R4850:Ryr2	UTSW	13	11,745,752 (GRCm38)	missense	probably damaging	1.00
R4850:Ryr2	UTSW	13	11,668,820 (GRCm38)	missense	probably damaging	0.99
R4880:Ryr2	UTSW	13	11,752,218 (GRCm38)	missense	probably damaging	1.00
R4917:Ryr2	UTSW	13	11,594,986 (GRCm38)	missense	probably damaging	0.96
R4918:Ryr2	UTSW	13	11,594,986 (GRCm38)	missense	probably damaging	0.96
R4922:Ryr2	UTSW	13	11,709,963 (GRCm38)	missense	probably damaging	0.99
R4933:Ryr2	UTSW	13	11,945,945 (GRCm38)	missense	probably damaging	0.96
R4950:Ryr2	UTSW	13	11,742,011 (GRCm38)	missense	probably damaging	1.00
R4957:Ryr2	UTSW	13	11,785,080 (GRCm38)	missense	probably damaging	0.97
R4964:Ryr2	UTSW	13	11,833,992 (GRCm38)	missense	probably benign	0.00
R4964:Ryr2	UTSW	13	11,714,611 (GRCm38)	missense	possibly damaging	0.49
R4966:Ryr2	UTSW	13	11,714,611 (GRCm38)	missense	possibly damaging	0.49
R4966:Ryr2	UTSW	13	11,833,992 (GRCm38)	missense	probably benign	0.00
R4997:Ryr2	UTSW	13	11,595,306 (GRCm38)	missense	probably benign	0.09
R4998:Ryr2	UTSW	13	11,643,895 (GRCm38)	missense	probably damaging	1.00
R5033:Ryr2	UTSW	13	11,587,254 (GRCm38)	missense	possibly damaging	0.93
R5061:Ryr2	UTSW	13	11,635,536 (GRCm38)	missense	possibly damaging	0.74
R5062:Ryr2	UTSW	13	11,700,354 (GRCm38)	missense	probably damaging	0.97
R5088:Ryr2	UTSW	13	11,712,243 (GRCm38)	nonsense	probably null
R5135:Ryr2	UTSW	13	11,662,130 (GRCm38)	missense	probably benign	0.05
R5138:Ryr2	UTSW	13	11,660,289 (GRCm38)	missense	probably damaging	1.00
R5168:Ryr2	UTSW	13	11,752,321 (GRCm38)	missense	probably benign
R5187:Ryr2	UTSW	13	11,772,452 (GRCm38)	missense	probably damaging	0.99
R5197:Ryr2	UTSW	13	11,638,430 (GRCm38)	critical splice donor site	probably null
R5262:Ryr2	UTSW	13	11,772,437 (GRCm38)	missense	probably damaging	0.99
R5325:Ryr2	UTSW	13	11,690,363 (GRCm38)	missense	probably damaging	0.97
R5381:Ryr2	UTSW	13	11,556,658 (GRCm38)	missense	probably damaging	1.00
R5437:Ryr2	UTSW	13	11,655,713 (GRCm38)	missense	probably damaging	1.00
R5477:Ryr2	UTSW	13	11,705,656 (GRCm38)	missense	probably damaging	1.00
R5497:Ryr2	UTSW	13	11,705,701 (GRCm38)	missense	probably null	0.15
R5509:Ryr2	UTSW	13	11,745,601 (GRCm38)	missense	probably damaging	0.98
R5518:Ryr2	UTSW	13	11,687,909 (GRCm38)	missense	probably benign	0.01
R5571:Ryr2	UTSW	13	11,555,448 (GRCm38)	missense	possibly damaging	0.91
R5591:Ryr2	UTSW	13	11,595,014 (GRCm38)	missense	probably benign	0.06
R5619:Ryr2	UTSW	13	11,708,202 (GRCm38)	missense	probably damaging	1.00
R5630:Ryr2	UTSW	13	11,601,805 (GRCm38)	missense	probably damaging	1.00
R5644:Ryr2	UTSW	13	11,595,582 (GRCm38)	missense	probably damaging	0.99
R5667:Ryr2	UTSW	13	11,759,836 (GRCm38)	missense	probably damaging	1.00
R5775:Ryr2	UTSW	13	11,769,962 (GRCm38)	missense	probably damaging	1.00
R5836:Ryr2	UTSW	13	11,603,732 (GRCm38)	missense	probably damaging	1.00
R5858:Ryr2	UTSW	13	11,560,574 (GRCm38)	missense	probably damaging	0.99
R5934:Ryr2	UTSW	13	11,584,154 (GRCm38)	missense	probably damaging	0.96
R5939:Ryr2	UTSW	13	11,790,332 (GRCm38)	missense	probably damaging	0.99
R5941:Ryr2	UTSW	13	11,687,902 (GRCm38)	missense	probably damaging	1.00
R5945:Ryr2	UTSW	13	11,660,122 (GRCm38)	missense	probably damaging	1.00
R5946:Ryr2	UTSW	13	11,726,953 (GRCm38)	missense	probably damaging	1.00
R5966:Ryr2	UTSW	13	11,662,238 (GRCm38)	nonsense	probably null
R5974:Ryr2	UTSW	13	11,714,511 (GRCm38)	splice site	probably null
R6104:Ryr2	UTSW	13	11,799,825 (GRCm38)	missense	probably damaging	1.00
R6118:Ryr2	UTSW	13	11,792,689 (GRCm38)	missense	possibly damaging	0.69
R6149:Ryr2	UTSW	13	11,669,017 (GRCm38)	missense	probably benign
R6208:Ryr2	UTSW	13	11,895,220 (GRCm38)	missense	probably benign	0.04
R6217:Ryr2	UTSW	13	11,834,078 (GRCm38)	missense	probably damaging	1.00
R6230:Ryr2	UTSW	13	11,660,107 (GRCm38)	missense	probably damaging	0.99
R6279:Ryr2	UTSW	13	11,680,999 (GRCm38)	missense	probably damaging	0.97
R6294:Ryr2	UTSW	13	11,879,496 (GRCm38)	missense	probably damaging	1.00
R6300:Ryr2	UTSW	13	11,680,999 (GRCm38)	missense	probably damaging	0.97
R6350:Ryr2	UTSW	13	11,761,396 (GRCm38)	missense	probably damaging	0.98
R6484:Ryr2	UTSW	13	11,662,383 (GRCm38)	missense	possibly damaging	0.90
R6489:Ryr2	UTSW	13	11,834,007 (GRCm38)	missense	probably benign	0.29
R6548:Ryr2	UTSW	13	11,668,821 (GRCm38)	missense	probably damaging	1.00
R6591:Ryr2	UTSW	13	11,594,723 (GRCm38)	missense	probably benign	0.01
R6623:Ryr2	UTSW	13	11,710,065 (GRCm38)	missense	probably damaging	1.00
R6649:Ryr2	UTSW	13	11,595,643 (GRCm38)	missense	probably damaging	0.99
R6691:Ryr2	UTSW	13	11,594,723 (GRCm38)	missense	probably benign	0.01
R6770:Ryr2	UTSW	13	11,738,462 (GRCm38)	missense	probably damaging	1.00
R6802:Ryr2	UTSW	13	11,686,966 (GRCm38)	missense	probably damaging	1.00
R6809:Ryr2	UTSW	13	11,726,930 (GRCm38)	missense	probably damaging	1.00
R6893:Ryr2	UTSW	13	11,829,654 (GRCm38)	missense	possibly damaging	0.75
R6911:Ryr2	UTSW	13	11,827,559 (GRCm38)	missense	possibly damaging	0.50
R6915:Ryr2	UTSW	13	11,745,601 (GRCm38)	missense	probably damaging	1.00
R6943:Ryr2	UTSW	13	11,566,948 (GRCm38)	missense	possibly damaging	0.92
R6960:Ryr2	UTSW	13	11,801,243 (GRCm38)	missense	probably benign	0.28
R6997:Ryr2	UTSW	13	11,654,380 (GRCm38)	missense	possibly damaging	0.88
R6998:Ryr2	UTSW	13	11,712,166 (GRCm38)	missense	probably damaging	0.99
R7001:Ryr2	UTSW	13	11,794,605 (GRCm38)	missense	probably damaging	0.98
R7047:Ryr2	UTSW	13	11,824,400 (GRCm38)	missense	possibly damaging	0.64
R7089:Ryr2	UTSW	13	11,649,776 (GRCm38)	missense	probably benign	0.10
R7125:Ryr2	UTSW	13	11,669,987 (GRCm38)	missense	probably damaging	0.99
R7127:Ryr2	UTSW	13	11,655,713 (GRCm38)	missense	probably damaging	1.00
R7131:Ryr2	UTSW	13	11,668,811 (GRCm38)	critical splice donor site	probably null
R7131:Ryr2	UTSW	13	11,640,327 (GRCm38)	missense	possibly damaging	0.63
R7159:Ryr2	UTSW	13	11,810,908 (GRCm38)	missense	probably damaging	0.99
R7174:Ryr2	UTSW	13	11,801,177 (GRCm38)	missense	possibly damaging	0.81
R7180:Ryr2	UTSW	13	11,686,978 (GRCm38)	missense	probably damaging	1.00
R7182:Ryr2	UTSW	13	11,759,757 (GRCm38)	missense	probably benign
R7189:Ryr2	UTSW	13	11,883,123 (GRCm38)	missense	probably damaging	1.00
R7241:Ryr2	UTSW	13	11,665,913 (GRCm38)	missense	possibly damaging	0.71
R7244:Ryr2	UTSW	13	11,597,146 (GRCm38)	missense	probably damaging	1.00
R7326:Ryr2	UTSW	13	11,738,194 (GRCm38)	missense	possibly damaging	0.95
R7331:Ryr2	UTSW	13	11,745,631 (GRCm38)	missense	probably benign
R7365:Ryr2	UTSW	13	11,640,275 (GRCm38)	missense	probably damaging	0.99
R7372:Ryr2	UTSW	13	11,680,999 (GRCm38)	missense	probably damaging	0.97
R7395:Ryr2	UTSW	13	11,785,111 (GRCm38)	missense	probably damaging	0.98
R7404:Ryr2	UTSW	13	11,735,620 (GRCm38)	missense	probably damaging	0.97
R7417:Ryr2	UTSW	13	11,556,748 (GRCm38)	splice site	probably null
R7425:Ryr2	UTSW	13	11,705,644 (GRCm38)	missense	probably benign	0.20
R7444:Ryr2	UTSW	13	11,555,463 (GRCm38)	missense	probably benign	0.25
R7456:Ryr2	UTSW	13	11,752,282 (GRCm38)	missense	probably benign
R7460:Ryr2	UTSW	13	11,705,710 (GRCm38)	missense	probably benign	0.10
R7474:Ryr2	UTSW	13	11,594,876 (GRCm38)	missense	probably benign	0.04
R7543:Ryr2	UTSW	13	11,638,431 (GRCm38)	critical splice donor site	probably null
R7549:Ryr2	UTSW	13	11,737,985 (GRCm38)	missense	probably benign	0.15
R7558:Ryr2	UTSW	13	11,799,825 (GRCm38)	missense	probably damaging	1.00
R7565:Ryr2	UTSW	13	11,560,653 (GRCm38)	missense	possibly damaging	0.84
R7627:Ryr2	UTSW	13	11,761,327 (GRCm38)	missense	possibly damaging	0.65
R7698:Ryr2	UTSW	13	11,761,315 (GRCm38)	missense	possibly damaging	0.94
R7702:Ryr2	UTSW	13	11,690,333 (GRCm38)	missense	probably damaging	0.99
R7719:Ryr2	UTSW	13	11,730,343 (GRCm38)	missense	possibly damaging	0.94
R7772:Ryr2	UTSW	13	11,751,011 (GRCm38)	missense	probably benign
R7797:Ryr2	UTSW	13	11,801,180 (GRCm38)	missense	probably damaging	0.99
R7829:Ryr2	UTSW	13	11,827,607 (GRCm38)	missense	possibly damaging	0.81
R7855:Ryr2	UTSW	13	11,706,623 (GRCm38)	nonsense	probably null
R7872:Ryr2	UTSW	13	11,595,724 (GRCm38)	missense	probably damaging	1.00
R7908:Ryr2	UTSW	13	11,792,748 (GRCm38)	missense	probably benign	0.01
R7929:Ryr2	UTSW	13	11,594,794 (GRCm38)	missense	probably damaging	1.00
R7929:Ryr2	UTSW	13	11,690,295 (GRCm38)	nonsense	probably null
R7952:Ryr2	UTSW	13	11,646,427 (GRCm38)	splice site	probably null
R8008:Ryr2	UTSW	13	11,657,094 (GRCm38)	missense	probably benign	0.30
R8011:Ryr2	UTSW	13	11,588,140 (GRCm38)	critical splice donor site	probably null
R8097:Ryr2	UTSW	13	11,945,995 (GRCm38)	missense	probably damaging	0.98
R8133:Ryr2	UTSW	13	11,603,698 (GRCm38)	missense	probably damaging	1.00
R8253:Ryr2	UTSW	13	11,827,553 (GRCm38)	missense	possibly damaging	0.94
R8278:Ryr2	UTSW	13	11,595,506 (GRCm38)	nonsense	probably null
R8351:Ryr2	UTSW	13	11,799,832 (GRCm38)	missense	probably damaging	0.98
R8401:Ryr2	UTSW	13	11,668,935 (GRCm38)	missense	possibly damaging	0.95
R8403:Ryr2	UTSW	13	11,684,478 (GRCm38)	missense	possibly damaging	0.95
R8431:Ryr2	UTSW	13	11,659,008 (GRCm38)	missense	probably benign	0.00
R8509:Ryr2	UTSW	13	11,577,778 (GRCm38)	critical splice donor site	probably null
R8551:Ryr2	UTSW	13	11,560,593 (GRCm38)	missense	possibly damaging	0.93
R8684:Ryr2	UTSW	13	11,687,989 (GRCm38)	missense	probably damaging	0.99
R8735:Ryr2	UTSW	13	11,686,947 (GRCm38)	missense	probably damaging	0.97
R8766:Ryr2	UTSW	13	11,668,969 (GRCm38)	missense	probably damaging	0.97
R8817:Ryr2	UTSW	13	11,735,623 (GRCm38)	missense	possibly damaging	0.95
R8827:Ryr2	UTSW	13	11,558,048 (GRCm38)	missense	possibly damaging	0.80
R8884:Ryr2	UTSW	13	11,779,266 (GRCm38)	missense	probably benign	0.19
R8889:Ryr2	UTSW	13	11,785,104 (GRCm38)	missense	probably damaging	0.99
R8891:Ryr2	UTSW	13	11,799,882 (GRCm38)	missense	probably damaging	1.00
R8979:Ryr2	UTSW	13	11,595,038 (GRCm38)	missense	probably benign	0.00
R9013:Ryr2	UTSW	13	11,603,732 (GRCm38)	missense	probably damaging	0.98
R9040:Ryr2	UTSW	13	11,594,786 (GRCm38)	missense	probably damaging	0.97
R9044:Ryr2	UTSW	13	11,738,103 (GRCm38)	nonsense	probably null
R9056:Ryr2	UTSW	13	11,595,931 (GRCm38)	missense	possibly damaging	0.94
R9084:Ryr2	UTSW	13	11,601,838 (GRCm38)	missense	probably damaging	1.00
R9113:Ryr2	UTSW	13	11,603,855 (GRCm38)	intron	probably benign
R9116:Ryr2	UTSW	13	11,572,299 (GRCm38)	missense	possibly damaging	0.93
R9125:Ryr2	UTSW	13	11,654,406 (GRCm38)	missense	probably benign	0.28
R9148:Ryr2	UTSW	13	11,885,538 (GRCm38)	missense	probably benign	0.02
R9210:Ryr2	UTSW	13	11,829,674 (GRCm38)	missense	probably damaging	0.99
R9212:Ryr2	UTSW	13	11,829,674 (GRCm38)	missense	probably damaging	0.99
R9233:Ryr2	UTSW	13	11,595,886 (GRCm38)	missense	possibly damaging	0.77
R9254:Ryr2	UTSW	13	11,883,116 (GRCm38)	missense	probably damaging	1.00
R9262:Ryr2	UTSW	13	11,750,968 (GRCm38)	missense	probably damaging	0.97
R9275:Ryr2	UTSW	13	11,883,090 (GRCm38)	missense	probably benign	0.10
R9278:Ryr2	UTSW	13	11,883,090 (GRCm38)	missense	probably benign	0.10
R9309:Ryr2	UTSW	13	11,706,692 (GRCm38)	missense	probably damaging	0.99
R9379:Ryr2	UTSW	13	11,883,116 (GRCm38)	missense	probably damaging	1.00
R9409:Ryr2	UTSW	13	11,681,087 (GRCm38)	missense	probably damaging	0.99
R9429:Ryr2	UTSW	13	11,794,573 (GRCm38)	missense	probably damaging	0.97
R9445:Ryr2	UTSW	13	11,772,577 (GRCm38)	missense	probably damaging	1.00
R9464:Ryr2	UTSW	13	11,737,794 (GRCm38)	missense	probably benign	0.00
R9467:Ryr2	UTSW	13	11,556,604 (GRCm38)	missense	possibly damaging	0.70
R9546:Ryr2	UTSW	13	11,587,215 (GRCm38)	critical splice donor site	probably null
R9562:Ryr2	UTSW	13	11,745,218 (GRCm38)	missense	probably damaging	1.00
R9609:Ryr2	UTSW	13	11,668,962 (GRCm38)	missense	probably damaging	1.00
R9704:Ryr2	UTSW	13	11,722,760 (GRCm38)	missense	probably damaging	1.00
R9764:Ryr2	UTSW	13	11,687,049 (GRCm38)	missense	possibly damaging	0.67
R9772:Ryr2	UTSW	13	11,594,899 (GRCm38)	missense	probably benign	0.13
R9776:Ryr2	UTSW	13	11,692,713 (GRCm38)	missense	probably damaging	0.98
S24628:Ryr2	UTSW	13	11,869,156 (GRCm38)	missense	probably damaging	0.97
X0019:Ryr2	UTSW	13	11,703,501 (GRCm38)	missense	probably benign	0.04
Z1176:Ryr2	UTSW	13	11,643,803 (GRCm38)	critical splice donor site	probably null
Z1176:Ryr2	UTSW	13	11,598,611 (GRCm38)	critical splice acceptor site	probably null
Z1176:Ryr2	UTSW	13	11,794,549 (GRCm38)	nonsense	probably null
Z1177:Ryr2	UTSW	13	11,750,873 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTGAGCTTTGGTAAGGGAGC -3'
(R):5'- CCTTGAGCTTGGATTTCTAAACC -3'

Sequencing Primer
(F):5'- GCATCCCTTAGAGAGTCTATATACAG -3'
(R):5'- CCACTTGAGAAGATAGATGATGTTGC -3'

Posted On

2016-02-04