|Institutional Source||Beutler Lab|
|Gene Name||kinesin family member 3B|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R4806 (G1)|
|Chromosomal Location||153291413-153333390 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||G to A at 153320368 bp|
|Amino Acid Change||Alanine to Threonine at position 500 (A500T)|
|Ref Sequence||ENSEMBL: ENSMUSP00000028977 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028977]|
|Predicted Effect||probably damaging
AA Change: A500T
PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
AA Change: A500T
|Meta Mutation Damage Score||0.4246|
|Coding Region Coverage||
|Validation Efficiency||100% (66/66)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene acts as a heterodimer with kinesin family member 3A to aid in chromosome movement during mitosis and meiosis. The encoded protein is a plus end-directed microtubule motor and can interact with the SMC3 subunit of the cohesin complex. In addition, the encoded protein may be involved in the intracellular movement of membranous organelles. This protein and kinesin family member 3A form the kinesin II subfamily of the kinesin superfamily. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygoous for a targeted null mutation are growth retarded and display neural tube defects, incomplete embryo turning, randomized left-right assymetry, pericardial edema, and die during the midgestational period. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Kif3b||
(F):5'- GCATAAGCCTGTTCTCTAGGTC -3'
(R):5'- TCTCGACGTTTCTGAGCAATAC -3'
(F):5'- ATAAGCCTGTTCTCTAGGTCTTCCTG -3'
(R):5'- AGCTTGCCCTAGTGACCATG -3'