Mutagenetix > Incidental Mutation

Incidental Mutation 'R4823:Ascc3'

371309

Institutional Source

Beutler Lab

Gene Symbol

Ascc3

Ensembl Gene

ENSMUSG00000038774

Gene Name

activating signal cointegrator 1 complex subunit 3

Synonyms

B630009I04Rik, ASC1p200, Helic1

MMRRC Submission

042439-MU

Accession Numbers

Ncbi RefSeq: NM_198007.2; MGI:1925237

Essential gene?

Probably essential (E-score: 0.963) question?

Stock #

R4823 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50592669-50851485 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 50713233 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 1017 (S1017P)

Ref Sequence

ENSEMBL: ENSMUSP00000036726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035606]

AlphaFold

E9PZJ8

Predicted Effect

probably damaging
Transcript: ENSMUST00000035606
AA Change: S1017P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000036726
Gene: ENSMUSG00000038774
AA Change: S1017P

Domain	Start	End	E-Value	Type
coiled coil region	55	79	N/A	INTRINSIC
low complexity region	124	135	N/A	INTRINSIC
coiled coil region	329	356	N/A	INTRINSIC
DEXDc	474	686	1.71e-29	SMART
AAA	492	674	8.15e-2	SMART
Blast:DEXDc	718	763	4e-18	BLAST
HELICc	770	858	6.01e-16	SMART
Sec63	979	1288	3.53e-111	SMART
DEXDc	1324	1528	8.88e-28	SMART
AAA	1342	1492	4.27e-1	SMART
HELICc	1605	1695	2.28e-16	SMART
Sec63	1813	2178	6.37e-118	SMART

Meta Mutation Damage Score

0.7413

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

98% (96/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

All alleles(16) : Targeted(2) Gene trapped(14)

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	G	A	5: 87,972,598 (GRCm38)	D405N	probably benign	Het
4921517D22Rik	T	G	13: 59,690,904 (GRCm38)	E38A	probably damaging	Het
4930433I11Rik	A	T	7: 40,993,362 (GRCm38)	I152F	probably benign	Het
5830473C10Rik	T	A	5: 90,566,503 (GRCm38)	L124H	probably benign	Het
Aass	A	T	6: 23,107,691 (GRCm38)	D364E	probably benign	Het
Adamts2	A	G	11: 50,737,187 (GRCm38)	D238G	probably benign	Het
Aplf	G	A	6: 87,646,255 (GRCm38)	L302F	probably damaging	Het
Apol7b	G	T	15: 77,427,782 (GRCm38)		probably benign	Het
Arhgef12	A	G	9: 43,020,696 (GRCm38)	V165A	probably benign	Het
B230104I21Rik	T	A	4: 154,349,747 (GRCm38)		probably benign	Het
Bfsp2	C	A	9: 103,479,883 (GRCm38)	C115F	probably damaging	Het
Bhmt2	A	T	13: 93,663,290 (GRCm38)	W213R	probably benign	Het
C87499	T	C	4: 88,629,215 (GRCm38)	K160R	probably damaging	Het
Capn5	A	T	7: 98,126,441 (GRCm38)	V431E	probably damaging	Het
Ccdc88c	A	G	12: 100,930,543 (GRCm38)	Y1390H	probably damaging	Het
Ccr3	A	G	9: 124,028,681 (GRCm38)	T18A	probably damaging	Het
Cdh5	T	C	8: 104,142,669 (GRCm38)	S676P	probably benign	Het
Ceacam5	A	G	7: 17,757,744 (GRCm38)	T680A	possibly damaging	Het
Cebpd	G	A	16: 15,888,114 (GRCm38)	G264S	probably benign	Het
Cfd	T	C	10: 79,890,948 (GRCm38)	V8A	probably benign	Het
Cops9	C	T	1: 92,641,866 (GRCm38)		probably benign	Het
Cpne6	T	C	14: 55,517,010 (GRCm38)	Y533H	probably damaging	Het
Cyp2c67	T	A	19: 39,615,724 (GRCm38)	H396L	probably benign	Het
Cyp2j9	G	A	4: 96,568,735 (GRCm38)	P500S	possibly damaging	Het
Cyp4a12a	A	T	4: 115,327,413 (GRCm38)		probably null	Het
Dbt	G	A	3: 116,523,387 (GRCm38)	D71N	probably damaging	Het
Ddx41	G	T	13: 55,532,055 (GRCm38)	Q440K	probably benign	Het
Doxl2	A	G	6: 48,975,261 (GRCm38)	D40G	probably damaging	Het
Elovl4	A	G	9: 83,780,685 (GRCm38)	F174S	probably damaging	Het
Emcn	C	G	3: 137,423,426 (GRCm38)	P193R	probably damaging	Het
Etnk1	T	C	6: 143,167,638 (GRCm38)		probably null	Het
Fads3	A	C	19: 10,041,888 (GRCm38)	S53R	probably damaging	Het
Fam193a	A	G	5: 34,459,028 (GRCm38)	E849G	probably damaging	Het
Fat3	A	G	9: 15,996,507 (GRCm38)	V2733A	probably benign	Het
Frem3	T	A	8: 80,613,958 (GRCm38)	M960K	probably benign	Het
Frmd6	A	T	12: 70,872,575 (GRCm38)	I62L	probably benign	Het
Glmp	T	C	3: 88,325,223 (GRCm38)		probably benign	Het
Gm17421	T	C	12: 113,369,541 (GRCm38)		noncoding transcript	Het
Gm27013	T	A	6: 130,522,223 (GRCm38)		noncoding transcript	Het
Gtf2ird1	A	G	5: 134,395,722 (GRCm38)	V390A	probably damaging	Het
Hps3	A	G	3: 20,012,726 (GRCm38)	Y559H	probably benign	Het
Hsh2d	G	A	8: 72,200,460 (GRCm38)	D229N	probably benign	Het
Itpr3	A	G	17: 27,085,147 (GRCm38)	D114G	probably benign	Het
Jmjd4	G	A	11: 59,455,580 (GRCm38)	A408T	probably benign	Het
Kcnh4	G	T	11: 100,755,174 (GRCm38)	A316D	probably damaging	Het
Klrg1	T	A	6: 122,273,533 (GRCm38)		probably null	Het
Lancl2	T	A	6: 57,732,277 (GRCm38)	Y355N	probably damaging	Het
Ltb	T	C	17: 35,195,230 (GRCm38)	S115P	probably benign	Het
Mccc2	T	G	13: 100,000,254 (GRCm38)	R64S	probably benign	Het
Mgam2-ps	T	A	6: 40,832,662 (GRCm38)		noncoding transcript	Het
Mrrf	T	G	2: 36,148,030 (GRCm38)	N104K	possibly damaging	Het
Nipa1	C	A	7: 55,979,688 (GRCm38)	V226L	possibly damaging	Het
Numa1	T	A	7: 101,996,037 (GRCm38)	L290Q	probably damaging	Het
Ofcc1	T	A	13: 40,280,473 (GRCm38)	H52L	probably damaging	Het
Ogfod3	G	A	11: 121,195,201 (GRCm38)	A189V	probably benign	Het
Olfr1176	A	G	2: 88,339,835 (GRCm38)	D90G	probably damaging	Het
Olfr1444	A	T	19: 12,861,816 (GRCm38)	I14F	probably benign	Het
Olfr1505	G	A	19: 13,919,658 (GRCm38)	V213I	probably benign	Het
Olfr292	T	A	7: 86,694,588 (GRCm38)	I44N	probably damaging	Het
P2ry12	G	A	3: 59,217,897 (GRCm38)	S119L	probably benign	Het
Pde7b	T	A	10: 20,438,785 (GRCm38)	N192Y	probably damaging	Het
Pfkl	T	C	10: 77,997,594 (GRCm38)	N258S	probably damaging	Het
Phykpl	G	A	11: 51,586,593 (GRCm38)	A71T	probably damaging	Het
Ppp1r16a	T	C	15: 76,693,193 (GRCm38)		probably benign	Het
Pramef20	T	C	4: 144,373,211 (GRCm38)	N328S	possibly damaging	Het
Prune2	C	T	19: 17,120,504 (GRCm38)	T1124M	probably damaging	Het
Rapgef6	A	G	11: 54,694,500 (GRCm38)	I1570V	probably benign	Het
Rbm34	T	C	8: 126,970,905 (GRCm38)	S19G	probably benign	Het
Rnf10	T	C	5: 115,255,442 (GRCm38)		probably null	Het
Rnf34	A	G	5: 122,850,302 (GRCm38)		probably null	Het
Setd4	A	G	16: 93,589,950 (GRCm38)	S287P	probably benign	Het
Shc3	C	T	13: 51,451,570 (GRCm38)	V225I	probably benign	Het
Sipa1l3	C	T	7: 29,371,002 (GRCm38)	V1030I	probably damaging	Het
Siva1	G	T	12: 112,645,064 (GRCm38)	R33L	probably damaging	Het
Slc4a5	C	T	6: 83,272,133 (GRCm38)	T573I	probably damaging	Het
Sorcs1	C	T	19: 50,678,140 (GRCm38)	R110Q	possibly damaging	Het
Sorcs1	T	C	19: 50,230,302 (GRCm38)	I581V	possibly damaging	Het
Sorl1	T	C	9: 41,992,321 (GRCm38)	D1545G	probably damaging	Het
Tas2r124	T	G	6: 132,755,546 (GRCm38)	S273A	probably damaging	Het
Tcf15	T	C	2: 152,143,893 (GRCm38)	F90L	probably damaging	Het
Trim59	G	A	3: 69,037,120 (GRCm38)	R296C	probably benign	Het
Tulp1	A	T	17: 28,353,572 (GRCm38)	D229E	probably benign	Het
Ush1c	T	A	7: 46,195,733 (GRCm38)	N886I	probably benign	Het
Usp9y	A	T	Y: 1,444,559 (GRCm38)	S127T	probably damaging	Het
Vmn1r19	T	A	6: 57,405,234 (GRCm38)	Y257*	probably null	Het
Vmn2r109	A	T	17: 20,553,891 (GRCm38)	Y401N	probably damaging	Het
Vmn2r69	A	C	7: 85,411,300 (GRCm38)	S359A	probably benign	Het
Zfp37	A	T	4: 62,191,503 (GRCm38)	N479K	probably benign	Het

Other mutations in Ascc3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00264:Ascc3	APN	10	50,714,435 (GRCm38)	missense	probably damaging	0.99
IGL00690:Ascc3	APN	10	50,699,943 (GRCm38)	nonsense	probably null
IGL00897:Ascc3	APN	10	50,728,091 (GRCm38)	missense	probably benign	0.01
IGL01077:Ascc3	APN	10	50,649,317 (GRCm38)	splice site	probably benign
IGL01124:Ascc3	APN	10	50,732,473 (GRCm38)	missense	probably damaging	1.00
IGL01555:Ascc3	APN	10	50,750,522 (GRCm38)	missense	probably damaging	1.00
IGL02019:Ascc3	APN	10	50,690,139 (GRCm38)	missense	probably damaging	1.00
IGL02161:Ascc3	APN	10	50,850,527 (GRCm38)	nonsense	probably null
IGL02247:Ascc3	APN	10	50,650,590 (GRCm38)	missense	probably damaging	1.00
IGL02318:Ascc3	APN	10	50,728,154 (GRCm38)	nonsense	probably null
IGL02428:Ascc3	APN	10	50,845,695 (GRCm38)	nonsense	probably null
IGL02432:Ascc3	APN	10	50,700,493 (GRCm38)	missense	probably damaging	0.99
IGL02449:Ascc3	APN	10	50,700,599 (GRCm38)	missense	probably benign	0.00
IGL02640:Ascc3	APN	10	50,767,374 (GRCm38)	missense	possibly damaging	0.69
IGL02673:Ascc3	APN	10	50,660,673 (GRCm38)	missense	probably benign	0.01
IGL03144:Ascc3	APN	10	50,767,443 (GRCm38)	missense	probably benign	0.16
IGL03161:Ascc3	APN	10	50,618,072 (GRCm38)	missense	probably damaging	0.98
IGL03218:Ascc3	APN	10	50,823,853 (GRCm38)	missense	possibly damaging	0.89
algorithm	UTSW	10	50,718,376 (GRCm38)	missense	probably damaging	0.97
heuristic	UTSW	10	50,842,193 (GRCm38)	missense	probably damaging	0.99
network	UTSW	10	50,754,079 (GRCm38)	missense	possibly damaging	0.53
R0045:Ascc3	UTSW	10	50,718,402 (GRCm38)	nonsense	probably null
R0045:Ascc3	UTSW	10	50,718,402 (GRCm38)	nonsense	probably null
R0131:Ascc3	UTSW	10	50,735,329 (GRCm38)	missense	probably damaging	0.99
R0131:Ascc3	UTSW	10	50,735,329 (GRCm38)	missense	probably damaging	0.99
R0132:Ascc3	UTSW	10	50,735,329 (GRCm38)	missense	probably damaging	0.99
R0149:Ascc3	UTSW	10	50,607,993 (GRCm38)	missense	probably benign	0.31
R0165:Ascc3	UTSW	10	50,842,127 (GRCm38)	splice site	probably null
R0255:Ascc3	UTSW	10	50,645,058 (GRCm38)	missense	probably benign	0.00
R0310:Ascc3	UTSW	10	50,748,926 (GRCm38)	missense	probably benign	0.02
R0314:Ascc3	UTSW	10	50,637,999 (GRCm38)	missense	possibly damaging	0.92
R0362:Ascc3	UTSW	10	50,748,955 (GRCm38)	splice site	probably benign
R0418:Ascc3	UTSW	10	50,748,926 (GRCm38)	missense	probably benign	0.02
R0419:Ascc3	UTSW	10	50,748,926 (GRCm38)	missense	probably benign	0.02
R0421:Ascc3	UTSW	10	50,748,926 (GRCm38)	missense	probably benign	0.02
R0480:Ascc3	UTSW	10	50,735,252 (GRCm38)	missense	probably damaging	1.00
R0744:Ascc3	UTSW	10	50,845,666 (GRCm38)	missense	probably benign	0.17
R0833:Ascc3	UTSW	10	50,845,666 (GRCm38)	missense	probably benign	0.17
R1231:Ascc3	UTSW	10	50,823,660 (GRCm38)	missense	probably damaging	1.00
R1264:Ascc3	UTSW	10	50,642,519 (GRCm38)	splice site	probably benign
R1302:Ascc3	UTSW	10	50,604,794 (GRCm38)	start codon destroyed	probably null	1.00
R1751:Ascc3	UTSW	10	50,718,376 (GRCm38)	missense	probably damaging	0.97
R1767:Ascc3	UTSW	10	50,718,376 (GRCm38)	missense	probably damaging	0.97
R1769:Ascc3	UTSW	10	50,700,490 (GRCm38)	missense	probably damaging	1.00
R1840:Ascc3	UTSW	10	50,690,161 (GRCm38)	missense	probably benign	0.00
R1855:Ascc3	UTSW	10	50,617,922 (GRCm38)	missense	probably benign	0.01
R1953:Ascc3	UTSW	10	50,845,630 (GRCm38)	missense	probably benign
R1976:Ascc3	UTSW	10	50,649,166 (GRCm38)	missense	probably damaging	1.00
R2004:Ascc3	UTSW	10	50,617,742 (GRCm38)	missense	probably damaging	1.00
R2013:Ascc3	UTSW	10	50,649,812 (GRCm38)	missense	probably damaging	0.99
R2017:Ascc3	UTSW	10	50,690,211 (GRCm38)	missense	probably benign	0.00
R2040:Ascc3	UTSW	10	50,728,131 (GRCm38)	missense	probably benign
R2043:Ascc3	UTSW	10	50,700,520 (GRCm38)	missense	probably damaging	1.00
R2165:Ascc3	UTSW	10	50,721,839 (GRCm38)	missense	probably damaging	1.00
R2226:Ascc3	UTSW	10	50,754,052 (GRCm38)	missense	probably benign	0.07
R2310:Ascc3	UTSW	10	50,748,892 (GRCm38)	missense	probably benign	0.15
R2405:Ascc3	UTSW	10	50,731,678 (GRCm38)	missense	probably damaging	1.00
R2424:Ascc3	UTSW	10	50,618,201 (GRCm38)	missense	probably benign	0.14
R3410:Ascc3	UTSW	10	50,700,100 (GRCm38)	missense	probably damaging	1.00
R3617:Ascc3	UTSW	10	50,618,185 (GRCm38)	missense	probably benign	0.00
R3771:Ascc3	UTSW	10	50,720,718 (GRCm38)	splice site	probably benign
R3783:Ascc3	UTSW	10	50,728,254 (GRCm38)	missense	probably damaging	1.00
R3891:Ascc3	UTSW	10	50,842,193 (GRCm38)	missense	probably damaging	0.99
R3892:Ascc3	UTSW	10	50,842,193 (GRCm38)	missense	probably damaging	0.99
R4435:Ascc3	UTSW	10	50,721,885 (GRCm38)	missense	probably benign	0.14
R4509:Ascc3	UTSW	10	50,842,243 (GRCm38)	missense	probably benign	0.00
R4520:Ascc3	UTSW	10	50,660,670 (GRCm38)	missense	probably benign
R4521:Ascc3	UTSW	10	50,660,670 (GRCm38)	missense	probably benign
R4522:Ascc3	UTSW	10	50,660,670 (GRCm38)	missense	probably benign
R4524:Ascc3	UTSW	10	50,660,670 (GRCm38)	missense	probably benign
R4581:Ascc3	UTSW	10	50,711,025 (GRCm38)	missense	probably damaging	1.00
R4701:Ascc3	UTSW	10	50,720,664 (GRCm38)	missense	possibly damaging	0.66
R4704:Ascc3	UTSW	10	50,659,014 (GRCm38)	missense	probably benign	0.02
R4768:Ascc3	UTSW	10	50,700,499 (GRCm38)	missense	probably damaging	1.00
R4906:Ascc3	UTSW	10	50,749,131 (GRCm38)	missense	probably damaging	1.00
R4937:Ascc3	UTSW	10	50,823,798 (GRCm38)	missense	probably damaging	1.00
R5001:Ascc3	UTSW	10	50,823,648 (GRCm38)	missense	probably damaging	1.00
R5151:Ascc3	UTSW	10	50,637,963 (GRCm38)	missense	probably damaging	0.99
R5263:Ascc3	UTSW	10	50,716,661 (GRCm38)	missense	probably benign	0.00
R5302:Ascc3	UTSW	10	50,707,777 (GRCm38)	missense	probably benign	0.09
R5436:Ascc3	UTSW	10	50,658,983 (GRCm38)	missense	probably damaging	0.99
R5455:Ascc3	UTSW	10	50,849,583 (GRCm38)	missense	probably benign	0.06
R5474:Ascc3	UTSW	10	50,849,538 (GRCm38)	missense	probably benign	0.25
R5744:Ascc3	UTSW	10	50,710,881 (GRCm38)	missense	probably benign
R5781:Ascc3	UTSW	10	50,637,978 (GRCm38)	missense	probably damaging	1.00
R5850:Ascc3	UTSW	10	50,710,953 (GRCm38)	missense	probably damaging	1.00
R5867:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R5868:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R5869:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R6031:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R6031:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R6032:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R6032:Ascc3	UTSW	10	50,842,183 (GRCm38)	nonsense	probably null
R6109:Ascc3	UTSW	10	50,649,247 (GRCm38)	missense	probably benign	0.37
R6122:Ascc3	UTSW	10	50,617,925 (GRCm38)	missense	probably benign
R6128:Ascc3	UTSW	10	50,650,638 (GRCm38)	missense	probably damaging	1.00
R6351:Ascc3	UTSW	10	50,720,673 (GRCm38)	missense	probably damaging	0.99
R6368:Ascc3	UTSW	10	50,699,985 (GRCm38)	missense	probably damaging	1.00
R6369:Ascc3	UTSW	10	50,699,985 (GRCm38)	missense	probably damaging	1.00
R6409:Ascc3	UTSW	10	50,845,580 (GRCm38)	missense	probably benign	0.09
R6472:Ascc3	UTSW	10	50,720,687 (GRCm38)	missense	probably benign	0.03
R6474:Ascc3	UTSW	10	50,748,836 (GRCm38)	missense	probably benign	0.01
R6480:Ascc3	UTSW	10	50,710,953 (GRCm38)	missense	probably damaging	1.00
R6553:Ascc3	UTSW	10	50,842,177 (GRCm38)	missense	probably benign	0.05
R6572:Ascc3	UTSW	10	50,690,247 (GRCm38)	nonsense	probably null
R6585:Ascc3	UTSW	10	50,842,177 (GRCm38)	missense	probably benign	0.05
R6656:Ascc3	UTSW	10	50,649,925 (GRCm38)	nonsense	probably null
R6669:Ascc3	UTSW	10	50,840,373 (GRCm38)	missense	probably benign
R6675:Ascc3	UTSW	10	50,750,563 (GRCm38)	nonsense	probably null
R6790:Ascc3	UTSW	10	50,645,712 (GRCm38)	missense	probably damaging	1.00
R6856:Ascc3	UTSW	10	50,749,062 (GRCm38)	missense	probably damaging	1.00
R6862:Ascc3	UTSW	10	50,849,646 (GRCm38)	missense	probably null	0.51
R6919:Ascc3	UTSW	10	50,645,753 (GRCm38)	nonsense	probably null
R6936:Ascc3	UTSW	10	50,729,961 (GRCm38)	missense	probably damaging	0.98
R6953:Ascc3	UTSW	10	50,645,666 (GRCm38)	missense	probably benign	0.00
R6957:Ascc3	UTSW	10	50,728,182 (GRCm38)	missense	probably damaging	1.00
R7022:Ascc3	UTSW	10	50,716,629 (GRCm38)	missense	possibly damaging	0.55
R7050:Ascc3	UTSW	10	50,840,350 (GRCm38)	missense	probably benign	0.43
R7358:Ascc3	UTSW	10	50,714,352 (GRCm38)	nonsense	probably null
R7479:Ascc3	UTSW	10	50,649,799 (GRCm38)	missense	probably damaging	1.00
R7538:Ascc3	UTSW	10	50,845,700 (GRCm38)	missense	probably damaging	1.00
R7838:Ascc3	UTSW	10	50,728,297 (GRCm38)	missense	probably benign	0.04
R8021:Ascc3	UTSW	10	50,731,648 (GRCm38)	missense	probably benign	0.02
R8134:Ascc3	UTSW	10	50,767,458 (GRCm38)	missense	probably benign	0.02
R8252:Ascc3	UTSW	10	50,642,610 (GRCm38)	missense	probably benign
R8348:Ascc3	UTSW	10	50,618,077 (GRCm38)	missense	probably benign
R8351:Ascc3	UTSW	10	50,849,597 (GRCm38)	missense	probably benign
R8356:Ascc3	UTSW	10	50,649,907 (GRCm38)	missense	probably benign	0.38
R8362:Ascc3	UTSW	10	50,642,596 (GRCm38)	missense	possibly damaging	0.93
R8395:Ascc3	UTSW	10	50,649,304 (GRCm38)	missense	possibly damaging	0.93
R8448:Ascc3	UTSW	10	50,618,077 (GRCm38)	missense	probably benign
R8957:Ascc3	UTSW	10	50,700,112 (GRCm38)	missense	probably damaging	1.00
R9004:Ascc3	UTSW	10	50,842,180 (GRCm38)	missense	probably benign
R9133:Ascc3	UTSW	10	50,754,079 (GRCm38)	missense	possibly damaging	0.53
R9200:Ascc3	UTSW	10	50,645,691 (GRCm38)	missense	possibly damaging	0.55
R9328:Ascc3	UTSW	10	50,658,919 (GRCm38)	missense	probably damaging	1.00
R9377:Ascc3	UTSW	10	50,732,762 (GRCm38)	missense	possibly damaging	0.62
R9412:Ascc3	UTSW	10	50,649,134 (GRCm38)	missense	probably benign	0.00
R9576:Ascc3	UTSW	10	50,618,158 (GRCm38)	missense	possibly damaging	0.71
R9796:Ascc3	UTSW	10	50,732,705 (GRCm38)	nonsense	probably null
X0021:Ascc3	UTSW	10	50,700,590 (GRCm38)	missense	possibly damaging	0.88
X0025:Ascc3	UTSW	10	50,650,596 (GRCm38)	missense	probably benign	0.00
X0026:Ascc3	UTSW	10	50,732,478 (GRCm38)	missense	probably damaging	1.00
Z1177:Ascc3	UTSW	10	50,718,421 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTAGATTTTGCTAAATACCTGCC -3'
(R):5'- TTTTGGGCAAAAGCAACAAATGTTG -3'

Sequencing Primer
(F):5'- ATACCTGCCTTTATTTATATTCTCGC -3'
(R):5'- GAGGTATAATTTAGGATAGTGGA -3'

Posted On

2016-03-01