Mutagenetix > Incidental Mutation

Incidental Mutation 'R4825:Rapgef2'

371364

Institutional Source

Beutler Lab

Gene Symbol

Rapgef2

Ensembl Gene

ENSMUSG00000062232

Gene Name

Rap guanine nucleotide exchange factor (GEF) 2

Synonyms

CNRasGEF, nRapGEP, 5830453M24Rik, RA-GEF-1, Pdzgef1

MMRRC Submission

042441-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4825 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

78969823-79193824 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 78990534 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 915 (M915V)

Ref Sequence

ENSEMBL: ENSMUSP00000114119 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000118100] [ENSMUST00000118340] [ENSMUST00000195708]

AlphaFold

Q8CHG7

Predicted Effect

probably benign
Transcript: ENSMUST00000118100
AA Change: M915V

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000114119
Gene: ENSMUSG00000062232
AA Change: M915V

Domain	Start	End	E-Value	Type
low complexity region	38	62	N/A	INTRINSIC
low complexity region	84	95	N/A	INTRINSIC
cNMP	135	253	2.48e-15	SMART
RasGEFN	267	380	1.3e-31	SMART
PDZ	395	467	1.28e-12	SMART
RA	606	692	7.59e-23	SMART
RasGEF	713	950	6.09e-100	SMART
low complexity region	1030	1046	N/A	INTRINSIC
low complexity region	1110	1124	N/A	INTRINSIC
low complexity region	1140	1161	N/A	INTRINSIC
low complexity region	1392	1405	N/A	INTRINSIC
low complexity region	1440	1455	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000118340
AA Change: M913V

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000113778
Gene: ENSMUSG00000062232
AA Change: M913V

Domain	Start	End	E-Value	Type
low complexity region	36	60	N/A	INTRINSIC
low complexity region	82	93	N/A	INTRINSIC
cNMP	133	251	2.48e-15	SMART
RasGEFN	265	378	1.3e-31	SMART
PDZ	393	465	1.28e-12	SMART
RA	604	690	7.59e-23	SMART
RasGEF	711	948	6.09e-100	SMART
low complexity region	1028	1044	N/A	INTRINSIC
low complexity region	1108	1122	N/A	INTRINSIC
low complexity region	1138	1159	N/A	INTRINSIC
low complexity region	1390	1403	N/A	INTRINSIC
low complexity region	1438	1453	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000195708
AA Change: M1063V

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000141542
Gene: ENSMUSG00000062232
AA Change: M1063V

Domain	Start	End	E-Value	Type
cNMP	24	131	3.9e-4	SMART
low complexity region	186	210	N/A	INTRINSIC
low complexity region	232	243	N/A	INTRINSIC
cNMP	283	401	1.2e-17	SMART
RasGEFN	415	528	6.4e-34	SMART
PDZ	543	615	6.4e-15	SMART
RA	754	840	4.8e-25	SMART
RasGEF	861	1098	3.8e-102	SMART
low complexity region	1178	1194	N/A	INTRINSIC
low complexity region	1258	1272	N/A	INTRINSIC
low complexity region	1288	1309	N/A	INTRINSIC
low complexity region	1540	1553	N/A	INTRINSIC
low complexity region	1588	1603	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Homozygotes for a null allele die at mid-gestation exhibiting growth arrest and defects in vascular development, neural tube closure and embryo turning. Homozygotes for another null allele show yolk sac vascular defects, impaired cell physiology and heart, primitive gut, liver and brain formation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 130 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	C	A	11: 109,682,498 (GRCm39)	L229F	probably benign	Het
A930002H24Rik	A	C	17: 64,170,603 (GRCm39)	S62A	unknown	Het
Abca12	T	A	1: 71,341,844 (GRCm39)	Q1039L	possibly damaging	Het
Adgrg5	T	A	8: 95,668,362 (GRCm39)	F476I	possibly damaging	Het
Alms1	A	G	6: 85,655,227 (GRCm39)	K2789E	probably damaging	Het
Arrdc2	G	A	8: 71,291,921 (GRCm39)		probably null	Het
Atp2b1	T	A	10: 98,845,426 (GRCm39)	I743K	probably damaging	Het
Atp6v1c2	C	T	12: 17,339,061 (GRCm39)	G230D	probably benign	Het
AU040320	G	A	4: 126,685,586 (GRCm39)	C54Y	probably damaging	Het
BC024139	C	T	15: 76,004,517 (GRCm39)	V680I	possibly damaging	Het
Bckdhb	A	G	9: 83,870,958 (GRCm39)	D156G	probably damaging	Het
Bltp3a	G	A	17: 28,096,368 (GRCm39)	A107T	probably damaging	Het
Canx	T	A	11: 50,199,636 (GRCm39)	D143V	probably benign	Het
Ccdc180	T	A	4: 45,912,794 (GRCm39)	V591E	possibly damaging	Het
Ccno	T	C	13: 113,124,633 (GRCm39)	S68P	probably benign	Het
Cdc45	C	T	16: 18,603,613 (GRCm39)	E527K	probably damaging	Het
Cep290	T	A	10: 100,324,210 (GRCm39)	D14E	probably damaging	Het
Cgnl1	A	G	9: 71,537,806 (GRCm39)	V1238A	probably benign	Het
Ciz1	C	T	2: 32,261,753 (GRCm39)	A455V	probably damaging	Het
Coro2b	T	A	9: 62,361,905 (GRCm39)	Y86F	probably benign	Het
Csf2ra	C	T	19: 61,214,990 (GRCm39)	R158Q	probably benign	Het
Cubn	A	T	2: 13,330,036 (GRCm39)	I2615N	probably damaging	Het
Dhx8	C	T	11: 101,628,996 (GRCm39)	R129*	probably null	Het
Disc1	T	A	8: 125,862,041 (GRCm39)	M471K	possibly damaging	Het
Dmxl2	G	T	9: 54,311,325 (GRCm39)	L1799I	probably benign	Het
Dnah2	A	G	11: 69,314,031 (GRCm39)	S4108P	probably damaging	Het
Ecpas	G	A	4: 58,850,911 (GRCm39)	L421F	probably damaging	Het
Ehmt2	C	G	17: 35,125,940 (GRCm39)	P211R	probably benign	Het
Eif4g3	A	G	4: 137,921,392 (GRCm39)	D1557G	probably benign	Het
Epha5	T	C	5: 84,381,699 (GRCm39)	D384G	probably damaging	Het
Etl4	A	G	2: 20,811,738 (GRCm39)	I1274V	probably damaging	Het
Fhip1a	G	A	3: 85,580,739 (GRCm39)	P489S	possibly damaging	Het
Fip1l1	G	A	5: 74,748,866 (GRCm39)		probably null	Het
Fubp1	T	C	3: 151,923,527 (GRCm39)		probably null	Het
Glul	T	C	1: 153,778,790 (GRCm39)	V33A	probably benign	Het
Gm4846	A	G	1: 166,319,237 (GRCm39)	F167S	probably damaging	Het
Heatr6	T	A	11: 83,649,148 (GRCm39)	L168M	probably damaging	Het
Hif1a	T	A	12: 73,979,175 (GRCm39)	I233N	probably damaging	Het
Hivep2	T	A	10: 14,007,063 (GRCm39)	H1220Q	possibly damaging	Het
Igkv8-21	T	C	6: 70,292,410 (GRCm39)	I9M	probably benign	Het
Izumo1	T	A	7: 45,274,411 (GRCm39)	C62*	probably null	Het
Jakmip3	G	A	7: 138,628,495 (GRCm39)	E424K	probably damaging	Het
Klhl2	A	G	8: 65,205,847 (GRCm39)	V358A	probably damaging	Het
Klk10	C	G	7: 43,433,022 (GRCm39)	D139E	probably damaging	Het
Klk14	T	C	7: 43,341,500 (GRCm39)	C51R	probably damaging	Het
Klk1b5	T	G	7: 43,494,814 (GRCm39)	I99S	probably damaging	Het
L3hypdh	T	C	12: 72,124,167 (GRCm39)	T258A	probably benign	Het
Lrp5	A	G	19: 3,664,292 (GRCm39)	Y812H	probably damaging	Het
Lrrc40	T	A	3: 157,766,967 (GRCm39)	L474*	probably null	Het
Mkks	A	T	2: 136,722,575 (GRCm39)	M194K	probably benign	Het
Mmp20	A	G	9: 7,654,121 (GRCm39)	D347G	probably damaging	Het
Mmp27	A	G	9: 7,581,195 (GRCm39)	E460G	probably damaging	Het
Mms22l	T	C	4: 24,536,226 (GRCm39)	F605S	probably damaging	Het
Mpzl3	A	G	9: 44,979,627 (GRCm39)	S193G	probably benign	Het
Muc4	A	T	16: 32,570,565 (GRCm39)	T542S	probably benign	Het
Muc5b	T	C	7: 141,422,202 (GRCm39)	L4446P	possibly damaging	Het
Mxi1	C	A	19: 53,358,769 (GRCm39)	S131*	probably null	Het
Nanog	G	T	6: 122,690,299 (GRCm39)	A210S	probably benign	Het
Nrcam	C	T	12: 44,622,769 (GRCm39)	Q988*	probably null	Het
Nsg1	C	A	5: 38,316,391 (GRCm39)		probably benign	Het
Ogfod3	G	A	11: 121,086,027 (GRCm39)	A189V	probably benign	Het
Or10g9b	A	T	9: 39,918,038 (GRCm39)	M69K	possibly damaging	Het
Or12e9	T	A	2: 87,202,432 (GRCm39)	C185*	probably null	Het
Or4a68	A	G	2: 89,270,209 (GRCm39)	V138A	probably benign	Het
Or4c122	T	C	2: 89,079,034 (GRCm39)		probably null	Het
Or4c35	G	A	2: 89,808,497 (GRCm39)	C125Y	probably damaging	Het
Or51ai2	T	C	7: 103,586,710 (GRCm39)	V41A	probably benign	Het
Or52b4i	T	A	7: 102,191,587 (GRCm39)	V148E	possibly damaging	Het
Or5b111	A	T	19: 13,291,684 (GRCm39)		probably null	Het
Or7e165	T	G	9: 19,694,872 (GRCm39)	S148A	possibly damaging	Het
Or8b53	A	T	9: 38,667,703 (GRCm39)	T240S	probably damaging	Het
Orc3	A	T	4: 34,571,774 (GRCm39)	M665K	possibly damaging	Het
Pabpc1	A	G	15: 36,597,255 (GRCm39)	S591P	probably damaging	Het
Pacc1	A	T	1: 191,073,040 (GRCm39)	I154F	probably damaging	Het
Parp6	T	A	9: 59,531,645 (GRCm39)		probably null	Het
Pcdh1	T	C	18: 38,322,912 (GRCm39)	M974V	possibly damaging	Het
Pcdhb22	T	A	18: 37,653,713 (GRCm39)	V727E	possibly damaging	Het
Pex5l	T	C	3: 33,047,134 (GRCm39)	E272G	probably damaging	Het
Pglyrp2	G	T	17: 32,637,235 (GRCm39)	N264K	probably benign	Het
Phxr4	T	A	9: 13,342,882 (GRCm39)		probably benign	Het
Piezo2	A	G	18: 63,278,025 (GRCm39)	F293S	probably damaging	Het
Pkhd1	T	C	1: 20,607,625 (GRCm39)	D1077G	probably damaging	Het
Plekhs1	A	G	19: 56,461,700 (GRCm39)		probably null	Het
Prex1	G	T	2: 166,427,777 (GRCm39)	C788*	probably null	Het
Prrt3	A	T	6: 113,475,099 (GRCm39)	M41K	probably benign	Het
Ptgir	T	C	7: 16,642,768 (GRCm39)	V326A	probably damaging	Het
Ptprg	T	A	14: 12,220,654 (GRCm38)	D455E	probably damaging	Het
Ptpru	T	A	4: 131,526,914 (GRCm39)	Q686L	probably benign	Het
Pxdc1	G	T	13: 34,814,343 (GRCm39)	T190K	probably benign	Het
Rap1b	A	T	10: 117,654,487 (GRCm39)	C118S	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rpe65	C	T	3: 159,330,318 (GRCm39)	A495V	probably benign	Het
Samd15	A	G	12: 87,247,608 (GRCm39)	T98A	possibly damaging	Het
Sdk1	T	G	5: 141,568,049 (GRCm39)	D82E	probably benign	Het
Slc26a7	T	C	4: 14,546,309 (GRCm39)	D340G	probably benign	Het
Slc6a15	T	A	10: 103,253,921 (GRCm39)	M619K	probably benign	Het
Slc7a4	C	A	16: 17,392,385 (GRCm39)	D350Y	probably damaging	Het
Slk	T	G	19: 47,608,395 (GRCm39)	N449K	probably benign	Het
Spta1	A	T	1: 174,071,608 (GRCm39)		probably null	Het
Sptbn5	A	T	2: 119,886,374 (GRCm39)		probably benign	Het
Srd5a2	A	T	17: 74,354,800 (GRCm39)	V8D	probably benign	Het
Srgap3	G	A	6: 112,704,271 (GRCm39)	A906V	probably benign	Het
Stab2	A	T	10: 86,783,011 (GRCm39)	M679K	probably benign	Het
Stk3	T	C	15: 35,000,054 (GRCm39)	I291V	probably benign	Het
Supt6	T	A	11: 78,098,960 (GRCm39)	Q1637L	possibly damaging	Het
Svil	T	C	18: 5,114,564 (GRCm39)	F2047S	probably damaging	Het
Swsap1	A	G	9: 21,867,284 (GRCm39)	E76G	probably benign	Het
Syndig1	G	T	2: 149,741,473 (GRCm39)	G20C	probably damaging	Het
Synj2bp	A	T	12: 81,548,926 (GRCm39)	N104K	probably damaging	Het
Synpo2	T	A	3: 122,908,068 (GRCm39)	D416V	probably damaging	Het
Tacc3	G	T	5: 33,829,357 (GRCm39)	C620F	probably damaging	Het
Tanc1	A	G	2: 59,529,766 (GRCm39)	E19G	probably damaging	Het
Tars3	C	T	7: 65,297,302 (GRCm39)	A139V	probably benign	Het
Tbc1d22a	T	A	15: 86,235,935 (GRCm39)	C365S	probably damaging	Het
Tdpoz2	T	C	3: 93,559,381 (GRCm39)	H197R	possibly damaging	Het
Tha1	A	T	11: 117,760,205 (GRCm39)	N300K	probably damaging	Het
Tm6sf1	T	A	7: 81,515,008 (GRCm39)	F70L	probably damaging	Het
Tmem132b	T	A	5: 125,860,497 (GRCm39)	S581T	probably benign	Het
Tnfsf13	G	T	11: 69,576,075 (GRCm39)	S4*	probably null	Het
Tonsl	G	A	15: 76,517,448 (GRCm39)	S757L	probably benign	Het
Trem2	G	T	17: 48,658,719 (GRCm39)	R161S	possibly damaging	Het
Trpm2	A	T	10: 77,777,007 (GRCm39)	V430E	probably damaging	Het
Ttc14	T	A	3: 33,855,518 (GRCm39)	D154E	possibly damaging	Het
Ugt2b37	T	C	5: 87,398,498 (GRCm39)	M313V	possibly damaging	Het
Vmn2r24	A	G	6: 123,792,739 (GRCm39)	I689V	probably benign	Het
Wdr76	A	G	2: 121,372,975 (GRCm39)	T601A	probably benign	Het
Xirp1	T	C	9: 119,846,069 (GRCm39)	D938G	possibly damaging	Het
Zfp608	A	T	18: 55,031,041 (GRCm39)	D966E	probably benign	Het
Zfp957	A	G	14: 79,451,796 (GRCm39)	M1T	probably null	Het
Zkscan16	A	T	4: 58,957,809 (GRCm39)	H697L	possibly damaging	Het

Other mutations in Rapgef2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00488:Rapgef2	APN	3	78,999,332 (GRCm39)	missense	possibly damaging	0.89
IGL01024:Rapgef2	APN	3	78,977,445 (GRCm39)	missense	probably benign	0.43
IGL01448:Rapgef2	APN	3	79,011,269 (GRCm39)	critical splice donor site	probably null
IGL01448:Rapgef2	APN	3	78,976,244 (GRCm39)	missense	probably benign
IGL01928:Rapgef2	APN	3	79,011,270 (GRCm39)	missense	probably damaging	1.00
IGL01973:Rapgef2	APN	3	78,999,116 (GRCm39)	splice site	probably null
IGL02015:Rapgef2	APN	3	78,999,371 (GRCm39)	splice site	probably benign
IGL02498:Rapgef2	APN	3	78,974,060 (GRCm39)	missense	probably damaging	0.97
IGL02631:Rapgef2	APN	3	78,990,533 (GRCm39)	missense	possibly damaging	0.77
IGL02835:Rapgef2	APN	3	79,000,293 (GRCm39)	splice site	probably benign
IGL02887:Rapgef2	APN	3	78,976,187 (GRCm39)	splice site	probably benign
IGL03030:Rapgef2	APN	3	78,981,614 (GRCm39)	critical splice donor site	probably null
IGL03035:Rapgef2	APN	3	79,001,731 (GRCm39)	missense	probably damaging	1.00
IGL03222:Rapgef2	APN	3	78,995,302 (GRCm39)	missense	probably damaging	1.00
IGL03227:Rapgef2	APN	3	78,999,920 (GRCm39)	splice site	probably benign
IGL03326:Rapgef2	APN	3	78,999,140 (GRCm39)	missense	probably damaging	0.96
IGL03335:Rapgef2	APN	3	79,006,492 (GRCm39)	missense	probably damaging	1.00
IGL03384:Rapgef2	APN	3	78,990,853 (GRCm39)	missense	probably damaging	1.00
Bulge	UTSW	3	78,986,439 (GRCm39)	missense	probably benign	0.01
Hai_phat	UTSW	3	78,993,266 (GRCm39)	missense	probably damaging	1.00
R0022:Rapgef2	UTSW	3	78,995,207 (GRCm39)	missense	probably damaging	1.00
R0022:Rapgef2	UTSW	3	78,995,207 (GRCm39)	missense	probably damaging	1.00
R0038:Rapgef2	UTSW	3	78,976,703 (GRCm39)	missense	probably benign	0.00
R0117:Rapgef2	UTSW	3	78,986,484 (GRCm39)	missense	probably benign	0.00
R0225:Rapgef2	UTSW	3	79,011,412 (GRCm39)	missense	probably damaging	0.99
R0723:Rapgef2	UTSW	3	78,986,481 (GRCm39)	missense	probably benign	0.20
R0788:Rapgef2	UTSW	3	79,006,502 (GRCm39)	missense	possibly damaging	0.59
R1311:Rapgef2	UTSW	3	78,990,854 (GRCm39)	missense	probably benign	0.12
R1374:Rapgef2	UTSW	3	78,995,275 (GRCm39)	missense	probably benign	0.08
R1507:Rapgef2	UTSW	3	78,988,600 (GRCm39)	splice site	probably benign
R1523:Rapgef2	UTSW	3	79,000,056 (GRCm39)	missense	probably damaging	1.00
R1753:Rapgef2	UTSW	3	78,996,098 (GRCm39)	missense	possibly damaging	0.65
R1759:Rapgef2	UTSW	3	78,974,038 (GRCm39)	missense	possibly damaging	0.89
R1766:Rapgef2	UTSW	3	79,000,010 (GRCm39)	missense	probably damaging	1.00
R2436:Rapgef2	UTSW	3	78,996,079 (GRCm39)	missense	possibly damaging	0.95
R3033:Rapgef2	UTSW	3	78,981,613 (GRCm39)	critical splice donor site	probably null
R3766:Rapgef2	UTSW	3	78,996,057 (GRCm39)	missense	probably benign	0.01
R4118:Rapgef2	UTSW	3	78,976,194 (GRCm39)	critical splice donor site	probably null
R4416:Rapgef2	UTSW	3	78,976,364 (GRCm39)	nonsense	probably null
R4722:Rapgef2	UTSW	3	78,976,480 (GRCm39)	missense	probably benign	0.00
R4743:Rapgef2	UTSW	3	79,080,375 (GRCm39)	missense	probably damaging	0.99
R4780:Rapgef2	UTSW	3	79,077,076 (GRCm39)	splice site	probably benign
R4861:Rapgef2	UTSW	3	78,981,743 (GRCm39)	missense	probably benign	0.01
R4861:Rapgef2	UTSW	3	78,981,743 (GRCm39)	missense	probably benign	0.01
R4900:Rapgef2	UTSW	3	78,981,670 (GRCm39)	missense	probably benign	0.02
R4943:Rapgef2	UTSW	3	78,971,854 (GRCm39)	missense	probably benign	0.00
R5291:Rapgef2	UTSW	3	78,977,366 (GRCm39)	missense	possibly damaging	0.64
R5369:Rapgef2	UTSW	3	78,976,739 (GRCm39)	missense	probably benign	0.00
R5413:Rapgef2	UTSW	3	78,995,173 (GRCm39)	missense	probably damaging	1.00
R5561:Rapgef2	UTSW	3	78,995,950 (GRCm39)	critical splice donor site	probably null
R5568:Rapgef2	UTSW	3	79,011,308 (GRCm39)	missense	probably damaging	1.00
R5642:Rapgef2	UTSW	3	79,002,157 (GRCm39)	missense	probably damaging	1.00
R5783:Rapgef2	UTSW	3	78,995,300 (GRCm39)	missense	probably benign	0.00
R6041:Rapgef2	UTSW	3	78,976,469 (GRCm39)	missense	probably benign	0.00
R6193:Rapgef2	UTSW	3	78,976,751 (GRCm39)	missense	possibly damaging	0.48
R6324:Rapgef2	UTSW	3	78,986,439 (GRCm39)	missense	probably benign	0.01
R6551:Rapgef2	UTSW	3	79,122,342 (GRCm39)	splice site	probably null
R6688:Rapgef2	UTSW	3	78,976,435 (GRCm39)	missense	probably benign	0.03
R6908:Rapgef2	UTSW	3	79,011,370 (GRCm39)	missense	probably benign	0.01
R6913:Rapgef2	UTSW	3	78,993,281 (GRCm39)	missense	probably damaging	1.00
R6933:Rapgef2	UTSW	3	78,993,266 (GRCm39)	missense	probably damaging	1.00
R7086:Rapgef2	UTSW	3	78,993,353 (GRCm39)	missense	probably benign	0.08
R7106:Rapgef2	UTSW	3	78,973,915 (GRCm39)	missense	probably benign
R7228:Rapgef2	UTSW	3	78,976,525 (GRCm39)	missense	probably benign	0.03
R7242:Rapgef2	UTSW	3	78,995,210 (GRCm39)	nonsense	probably null
R7257:Rapgef2	UTSW	3	78,989,934 (GRCm39)	missense	probably damaging	0.99
R7322:Rapgef2	UTSW	3	79,053,130 (GRCm39)	start codon destroyed	probably null	0.02
R7443:Rapgef2	UTSW	3	78,988,531 (GRCm39)	missense	probably damaging	1.00
R7450:Rapgef2	UTSW	3	79,080,366 (GRCm39)	missense	probably benign	0.01
R7472:Rapgef2	UTSW	3	78,976,580 (GRCm39)	missense	probably benign	0.45
R7884:Rapgef2	UTSW	3	78,973,933 (GRCm39)	missense	possibly damaging	0.49
R7954:Rapgef2	UTSW	3	78,977,454 (GRCm39)	nonsense	probably null
R7957:Rapgef2	UTSW	3	79,122,276 (GRCm39)	missense	probably benign	0.27
R8071:Rapgef2	UTSW	3	79,000,343 (GRCm39)	missense	probably damaging	1.00
R8261:Rapgef2	UTSW	3	78,993,325 (GRCm39)	missense	probably benign	0.34
R8268:Rapgef2	UTSW	3	78,993,263 (GRCm39)	missense	probably benign	0.12
R8309:Rapgef2	UTSW	3	78,990,509 (GRCm39)	missense	possibly damaging	0.65
R8505:Rapgef2	UTSW	3	78,986,349 (GRCm39)	nonsense	probably null
R8783:Rapgef2	UTSW	3	79,005,651 (GRCm39)	missense	probably damaging	1.00
R8897:Rapgef2	UTSW	3	79,019,566 (GRCm39)	missense	probably damaging	1.00
R8965:Rapgef2	UTSW	3	78,999,851 (GRCm39)	missense	probably damaging	1.00
R9028:Rapgef2	UTSW	3	78,981,651 (GRCm39)	missense	probably damaging	1.00
R9284:Rapgef2	UTSW	3	79,000,010 (GRCm39)	missense	probably damaging	1.00
R9371:Rapgef2	UTSW	3	79,082,300 (GRCm39)	missense	probably damaging	1.00
R9479:Rapgef2	UTSW	3	79,019,495 (GRCm39)	missense	probably damaging	1.00
R9493:Rapgef2	UTSW	3	79,019,495 (GRCm39)	missense	probably damaging	1.00
R9494:Rapgef2	UTSW	3	79,019,495 (GRCm39)	missense	probably damaging	1.00
R9500:Rapgef2	UTSW	3	78,974,093 (GRCm39)	missense	probably benign
R9657:Rapgef2	UTSW	3	78,999,191 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGTCATATGTGCCCTGGATC -3'
(R):5'- CACGTTTCTCCATGAGGGTG -3'

Sequencing Primer
(F):5'- ATGTGCCCTGGATCCAATTCAGAG -3'
(R):5'- TTCTCCATGAGGGTGAGCGC -3'

Posted On

2016-03-01