Mutagenetix > Incidental Mutation

Incidental Mutation 'R4825:Eif4g3'

371381

Institutional Source

Beutler Lab

Gene Symbol

Eif4g3

Ensembl Gene

ENSMUSG00000028760

Gene Name

eukaryotic translation initiation factor 4 gamma, 3

Synonyms

4930523M17Rik, G1-419-52, repro8, 1500002J22Rik, eIF4GII

MMRRC Submission

042441-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.354) question?

Stock #

R4825 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

137719090-137934397 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 137921392 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1557 (D1557G)

Ref Sequence

ENSEMBL: ENSMUSP00000145147 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084214] [ENSMUST00000084215] [ENSMUST00000105831] [ENSMUST00000140796] [ENSMUST00000203828]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000084214
AA Change: D1373G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000081232
Gene: ENSMUSG00000028760
AA Change: D1373G

Domain	Start	End	E-Value	Type
low complexity region	24	43	N/A	INTRINSIC
low complexity region	93	120	N/A	INTRINSIC
low complexity region	147	152	N/A	INTRINSIC
PDB:1LJ2\|D	154	179	8e-9	PDB
low complexity region	192	207	N/A	INTRINSIC
low complexity region	269	310	N/A	INTRINSIC
low complexity region	427	444	N/A	INTRINSIC
low complexity region	534	550	N/A	INTRINSIC
low complexity region	579	588	N/A	INTRINSIC
low complexity region	592	616	N/A	INTRINSIC
Blast:MIF4G	617	708	5e-49	BLAST
Blast:MIF4G	722	765	5e-16	BLAST
MIF4G	768	996	1.42e-65	SMART
low complexity region	1086	1109	N/A	INTRINSIC
MA3	1215	1327	9.29e-38	SMART
eIF5C	1487	1574	7.92e-36	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000084215
AA Change: D1374G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000081233
Gene: ENSMUSG00000028760
AA Change: D1374G

Domain	Start	End	E-Value	Type
low complexity region	10	25	N/A	INTRINSIC
low complexity region	75	102	N/A	INTRINSIC
low complexity region	129	134	N/A	INTRINSIC
PDB:1LJ2\|D	136	161	8e-9	PDB
low complexity region	174	189	N/A	INTRINSIC
low complexity region	251	292	N/A	INTRINSIC
low complexity region	409	426	N/A	INTRINSIC
low complexity region	516	532	N/A	INTRINSIC
low complexity region	561	570	N/A	INTRINSIC
low complexity region	574	598	N/A	INTRINSIC
Blast:MIF4G	599	690	4e-49	BLAST
Blast:MIF4G	704	747	5e-16	BLAST
MIF4G	750	978	1.42e-65	SMART
low complexity region	1068	1113	N/A	INTRINSIC
MA3	1216	1328	9.29e-38	SMART
eIF5C	1488	1575	7.92e-36	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105831
AA Change: D1362G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000101457
Gene: ENSMUSG00000028760
AA Change: D1362G

Domain	Start	End	E-Value	Type
low complexity region	14	32	N/A	INTRINSIC
low complexity region	82	109	N/A	INTRINSIC
low complexity region	136	141	N/A	INTRINSIC
PDB:1LJ2\|D	143	168	8e-9	PDB
low complexity region	181	196	N/A	INTRINSIC
low complexity region	258	299	N/A	INTRINSIC
low complexity region	416	433	N/A	INTRINSIC
low complexity region	523	539	N/A	INTRINSIC
low complexity region	568	577	N/A	INTRINSIC
low complexity region	581	605	N/A	INTRINSIC
Blast:MIF4G	606	697	4e-49	BLAST
Blast:MIF4G	711	754	5e-16	BLAST
MIF4G	757	985	1.42e-65	SMART
low complexity region	1075	1098	N/A	INTRINSIC
MA3	1204	1316	9.29e-38	SMART
eIF5C	1476	1563	7.92e-36	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133902

Predicted Effect

probably benign
Transcript: ENSMUST00000140796
AA Change: D797G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000115946
Gene: ENSMUSG00000028760
AA Change: D797G

Domain	Start	End	E-Value	Type
low complexity region	3	12	N/A	INTRINSIC
low complexity region	16	40	N/A	INTRINSIC
Blast:MIF4G	41	132	2e-49	BLAST
Blast:MIF4G	146	189	3e-16	BLAST
MIF4G	192	420	1.42e-65	SMART
low complexity region	510	533	N/A	INTRINSIC
MA3	639	751	9.29e-38	SMART
eIF5C	911	998	7.92e-36	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000203828
AA Change: D1557G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000145147
Gene: ENSMUSG00000028760
AA Change: D1557G

Domain	Start	End	E-Value	Type
low complexity region	41	81	N/A	INTRINSIC
low complexity region	193	208	N/A	INTRINSIC
low complexity region	258	285	N/A	INTRINSIC
low complexity region	312	317	N/A	INTRINSIC
PDB:1LJ2\|D	319	344	9e-9	PDB
low complexity region	357	372	N/A	INTRINSIC
low complexity region	434	475	N/A	INTRINSIC
low complexity region	592	609	N/A	INTRINSIC
low complexity region	699	715	N/A	INTRINSIC
low complexity region	744	753	N/A	INTRINSIC
low complexity region	757	781	N/A	INTRINSIC
Blast:MIF4G	782	873	9e-49	BLAST
Blast:MIF4G	887	930	5e-16	BLAST
MIF4G	933	1161	6e-68	SMART
coiled coil region	1174	1201	N/A	INTRINSIC
low complexity region	1251	1296	N/A	INTRINSIC
MA3	1399	1511	3.9e-40	SMART
eIF5C	1671	1758	3.9e-38	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016]
PHENOTYPE: Mice homozygous for an ENU induced allele exhibit decreased testes weight, azoospermia, and arrested male meiosis. Mice homozygous for a gene trapped allele exhibit small testes. [provided by MGI curators]

Allele List at MGI

All alleles(27) : Gene trapped(27)

Other mutations in this stock

Total: 130 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	C	A	11: 109,682,498 (GRCm39)	L229F	probably benign	Het
A930002H24Rik	A	C	17: 64,170,603 (GRCm39)	S62A	unknown	Het
Abca12	T	A	1: 71,341,844 (GRCm39)	Q1039L	possibly damaging	Het
Adgrg5	T	A	8: 95,668,362 (GRCm39)	F476I	possibly damaging	Het
Alms1	A	G	6: 85,655,227 (GRCm39)	K2789E	probably damaging	Het
Arrdc2	G	A	8: 71,291,921 (GRCm39)		probably null	Het
Atp2b1	T	A	10: 98,845,426 (GRCm39)	I743K	probably damaging	Het
Atp6v1c2	C	T	12: 17,339,061 (GRCm39)	G230D	probably benign	Het
AU040320	G	A	4: 126,685,586 (GRCm39)	C54Y	probably damaging	Het
BC024139	C	T	15: 76,004,517 (GRCm39)	V680I	possibly damaging	Het
Bckdhb	A	G	9: 83,870,958 (GRCm39)	D156G	probably damaging	Het
Bltp3a	G	A	17: 28,096,368 (GRCm39)	A107T	probably damaging	Het
Canx	T	A	11: 50,199,636 (GRCm39)	D143V	probably benign	Het
Ccdc180	T	A	4: 45,912,794 (GRCm39)	V591E	possibly damaging	Het
Ccno	T	C	13: 113,124,633 (GRCm39)	S68P	probably benign	Het
Cdc45	C	T	16: 18,603,613 (GRCm39)	E527K	probably damaging	Het
Cep290	T	A	10: 100,324,210 (GRCm39)	D14E	probably damaging	Het
Cgnl1	A	G	9: 71,537,806 (GRCm39)	V1238A	probably benign	Het
Ciz1	C	T	2: 32,261,753 (GRCm39)	A455V	probably damaging	Het
Coro2b	T	A	9: 62,361,905 (GRCm39)	Y86F	probably benign	Het
Csf2ra	C	T	19: 61,214,990 (GRCm39)	R158Q	probably benign	Het
Cubn	A	T	2: 13,330,036 (GRCm39)	I2615N	probably damaging	Het
Dhx8	C	T	11: 101,628,996 (GRCm39)	R129*	probably null	Het
Disc1	T	A	8: 125,862,041 (GRCm39)	M471K	possibly damaging	Het
Dmxl2	G	T	9: 54,311,325 (GRCm39)	L1799I	probably benign	Het
Dnah2	A	G	11: 69,314,031 (GRCm39)	S4108P	probably damaging	Het
Ecpas	G	A	4: 58,850,911 (GRCm39)	L421F	probably damaging	Het
Ehmt2	C	G	17: 35,125,940 (GRCm39)	P211R	probably benign	Het
Epha5	T	C	5: 84,381,699 (GRCm39)	D384G	probably damaging	Het
Etl4	A	G	2: 20,811,738 (GRCm39)	I1274V	probably damaging	Het
Fhip1a	G	A	3: 85,580,739 (GRCm39)	P489S	possibly damaging	Het
Fip1l1	G	A	5: 74,748,866 (GRCm39)		probably null	Het
Fubp1	T	C	3: 151,923,527 (GRCm39)		probably null	Het
Glul	T	C	1: 153,778,790 (GRCm39)	V33A	probably benign	Het
Gm4846	A	G	1: 166,319,237 (GRCm39)	F167S	probably damaging	Het
Heatr6	T	A	11: 83,649,148 (GRCm39)	L168M	probably damaging	Het
Hif1a	T	A	12: 73,979,175 (GRCm39)	I233N	probably damaging	Het
Hivep2	T	A	10: 14,007,063 (GRCm39)	H1220Q	possibly damaging	Het
Igkv8-21	T	C	6: 70,292,410 (GRCm39)	I9M	probably benign	Het
Izumo1	T	A	7: 45,274,411 (GRCm39)	C62*	probably null	Het
Jakmip3	G	A	7: 138,628,495 (GRCm39)	E424K	probably damaging	Het
Klhl2	A	G	8: 65,205,847 (GRCm39)	V358A	probably damaging	Het
Klk10	C	G	7: 43,433,022 (GRCm39)	D139E	probably damaging	Het
Klk14	T	C	7: 43,341,500 (GRCm39)	C51R	probably damaging	Het
Klk1b5	T	G	7: 43,494,814 (GRCm39)	I99S	probably damaging	Het
L3hypdh	T	C	12: 72,124,167 (GRCm39)	T258A	probably benign	Het
Lrp5	A	G	19: 3,664,292 (GRCm39)	Y812H	probably damaging	Het
Lrrc40	T	A	3: 157,766,967 (GRCm39)	L474*	probably null	Het
Mkks	A	T	2: 136,722,575 (GRCm39)	M194K	probably benign	Het
Mmp20	A	G	9: 7,654,121 (GRCm39)	D347G	probably damaging	Het
Mmp27	A	G	9: 7,581,195 (GRCm39)	E460G	probably damaging	Het
Mms22l	T	C	4: 24,536,226 (GRCm39)	F605S	probably damaging	Het
Mpzl3	A	G	9: 44,979,627 (GRCm39)	S193G	probably benign	Het
Muc4	A	T	16: 32,570,565 (GRCm39)	T542S	probably benign	Het
Muc5b	T	C	7: 141,422,202 (GRCm39)	L4446P	possibly damaging	Het
Mxi1	C	A	19: 53,358,769 (GRCm39)	S131*	probably null	Het
Nanog	G	T	6: 122,690,299 (GRCm39)	A210S	probably benign	Het
Nrcam	C	T	12: 44,622,769 (GRCm39)	Q988*	probably null	Het
Nsg1	C	A	5: 38,316,391 (GRCm39)		probably benign	Het
Ogfod3	G	A	11: 121,086,027 (GRCm39)	A189V	probably benign	Het
Or10g9b	A	T	9: 39,918,038 (GRCm39)	M69K	possibly damaging	Het
Or12e9	T	A	2: 87,202,432 (GRCm39)	C185*	probably null	Het
Or4a68	A	G	2: 89,270,209 (GRCm39)	V138A	probably benign	Het
Or4c122	T	C	2: 89,079,034 (GRCm39)		probably null	Het
Or4c35	G	A	2: 89,808,497 (GRCm39)	C125Y	probably damaging	Het
Or51ai2	T	C	7: 103,586,710 (GRCm39)	V41A	probably benign	Het
Or52b4i	T	A	7: 102,191,587 (GRCm39)	V148E	possibly damaging	Het
Or5b111	A	T	19: 13,291,684 (GRCm39)		probably null	Het
Or7e165	T	G	9: 19,694,872 (GRCm39)	S148A	possibly damaging	Het
Or8b53	A	T	9: 38,667,703 (GRCm39)	T240S	probably damaging	Het
Orc3	A	T	4: 34,571,774 (GRCm39)	M665K	possibly damaging	Het
Pabpc1	A	G	15: 36,597,255 (GRCm39)	S591P	probably damaging	Het
Pacc1	A	T	1: 191,073,040 (GRCm39)	I154F	probably damaging	Het
Parp6	T	A	9: 59,531,645 (GRCm39)		probably null	Het
Pcdh1	T	C	18: 38,322,912 (GRCm39)	M974V	possibly damaging	Het
Pcdhb22	T	A	18: 37,653,713 (GRCm39)	V727E	possibly damaging	Het
Pex5l	T	C	3: 33,047,134 (GRCm39)	E272G	probably damaging	Het
Pglyrp2	G	T	17: 32,637,235 (GRCm39)	N264K	probably benign	Het
Phxr4	T	A	9: 13,342,882 (GRCm39)		probably benign	Het
Piezo2	A	G	18: 63,278,025 (GRCm39)	F293S	probably damaging	Het
Pkhd1	T	C	1: 20,607,625 (GRCm39)	D1077G	probably damaging	Het
Plekhs1	A	G	19: 56,461,700 (GRCm39)		probably null	Het
Prex1	G	T	2: 166,427,777 (GRCm39)	C788*	probably null	Het
Prrt3	A	T	6: 113,475,099 (GRCm39)	M41K	probably benign	Het
Ptgir	T	C	7: 16,642,768 (GRCm39)	V326A	probably damaging	Het
Ptprg	T	A	14: 12,220,654 (GRCm38)	D455E	probably damaging	Het
Ptpru	T	A	4: 131,526,914 (GRCm39)	Q686L	probably benign	Het
Pxdc1	G	T	13: 34,814,343 (GRCm39)	T190K	probably benign	Het
Rap1b	A	T	10: 117,654,487 (GRCm39)	C118S	probably benign	Het
Rapgef2	T	C	3: 78,990,534 (GRCm39)	M915V	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rpe65	C	T	3: 159,330,318 (GRCm39)	A495V	probably benign	Het
Samd15	A	G	12: 87,247,608 (GRCm39)	T98A	possibly damaging	Het
Sdk1	T	G	5: 141,568,049 (GRCm39)	D82E	probably benign	Het
Slc26a7	T	C	4: 14,546,309 (GRCm39)	D340G	probably benign	Het
Slc6a15	T	A	10: 103,253,921 (GRCm39)	M619K	probably benign	Het
Slc7a4	C	A	16: 17,392,385 (GRCm39)	D350Y	probably damaging	Het
Slk	T	G	19: 47,608,395 (GRCm39)	N449K	probably benign	Het
Spta1	A	T	1: 174,071,608 (GRCm39)		probably null	Het
Sptbn5	A	T	2: 119,886,374 (GRCm39)		probably benign	Het
Srd5a2	A	T	17: 74,354,800 (GRCm39)	V8D	probably benign	Het
Srgap3	G	A	6: 112,704,271 (GRCm39)	A906V	probably benign	Het
Stab2	A	T	10: 86,783,011 (GRCm39)	M679K	probably benign	Het
Stk3	T	C	15: 35,000,054 (GRCm39)	I291V	probably benign	Het
Supt6	T	A	11: 78,098,960 (GRCm39)	Q1637L	possibly damaging	Het
Svil	T	C	18: 5,114,564 (GRCm39)	F2047S	probably damaging	Het
Swsap1	A	G	9: 21,867,284 (GRCm39)	E76G	probably benign	Het
Syndig1	G	T	2: 149,741,473 (GRCm39)	G20C	probably damaging	Het
Synj2bp	A	T	12: 81,548,926 (GRCm39)	N104K	probably damaging	Het
Synpo2	T	A	3: 122,908,068 (GRCm39)	D416V	probably damaging	Het
Tacc3	G	T	5: 33,829,357 (GRCm39)	C620F	probably damaging	Het
Tanc1	A	G	2: 59,529,766 (GRCm39)	E19G	probably damaging	Het
Tars3	C	T	7: 65,297,302 (GRCm39)	A139V	probably benign	Het
Tbc1d22a	T	A	15: 86,235,935 (GRCm39)	C365S	probably damaging	Het
Tdpoz2	T	C	3: 93,559,381 (GRCm39)	H197R	possibly damaging	Het
Tha1	A	T	11: 117,760,205 (GRCm39)	N300K	probably damaging	Het
Tm6sf1	T	A	7: 81,515,008 (GRCm39)	F70L	probably damaging	Het
Tmem132b	T	A	5: 125,860,497 (GRCm39)	S581T	probably benign	Het
Tnfsf13	G	T	11: 69,576,075 (GRCm39)	S4*	probably null	Het
Tonsl	G	A	15: 76,517,448 (GRCm39)	S757L	probably benign	Het
Trem2	G	T	17: 48,658,719 (GRCm39)	R161S	possibly damaging	Het
Trpm2	A	T	10: 77,777,007 (GRCm39)	V430E	probably damaging	Het
Ttc14	T	A	3: 33,855,518 (GRCm39)	D154E	possibly damaging	Het
Ugt2b37	T	C	5: 87,398,498 (GRCm39)	M313V	possibly damaging	Het
Vmn2r24	A	G	6: 123,792,739 (GRCm39)	I689V	probably benign	Het
Wdr76	A	G	2: 121,372,975 (GRCm39)	T601A	probably benign	Het
Xirp1	T	C	9: 119,846,069 (GRCm39)	D938G	possibly damaging	Het
Zfp608	A	T	18: 55,031,041 (GRCm39)	D966E	probably benign	Het
Zfp957	A	G	14: 79,451,796 (GRCm39)	M1T	probably null	Het
Zkscan16	A	T	4: 58,957,809 (GRCm39)	H697L	possibly damaging	Het

Other mutations in Eif4g3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01817:Eif4g3	APN	4	137,847,673 (GRCm39)	missense	probably benign	0.01
IGL02171:Eif4g3	APN	4	137,853,900 (GRCm39)	missense	probably benign	0.03
IGL02487:Eif4g3	APN	4	137,930,689 (GRCm39)	missense	possibly damaging	0.92
IGL02514:Eif4g3	APN	4	137,853,505 (GRCm39)	missense	possibly damaging	0.87
IGL02622:Eif4g3	APN	4	137,824,677 (GRCm39)	splice site	probably benign
IGL02725:Eif4g3	APN	4	137,897,782 (GRCm39)	splice site	probably benign
IGL02735:Eif4g3	APN	4	137,853,522 (GRCm39)	missense	probably benign	0.40
IGL03008:Eif4g3	APN	4	137,847,699 (GRCm39)	missense	probably damaging	1.00
IGL03077:Eif4g3	APN	4	137,853,166 (GRCm39)	missense	probably damaging	1.00
N/A - 535:Eif4g3	UTSW	4	137,847,739 (GRCm39)	missense	probably damaging	0.98
R0013:Eif4g3	UTSW	4	137,903,159 (GRCm39)	missense	possibly damaging	0.88
R0193:Eif4g3	UTSW	4	137,873,687 (GRCm39)	splice site	probably benign
R0240:Eif4g3	UTSW	4	137,897,873 (GRCm39)	missense	probably damaging	0.98
R0240:Eif4g3	UTSW	4	137,897,873 (GRCm39)	missense	probably damaging	0.98
R0563:Eif4g3	UTSW	4	137,903,151 (GRCm39)	splice site	probably benign
R0841:Eif4g3	UTSW	4	137,893,129 (GRCm39)	missense	probably damaging	1.00
R0884:Eif4g3	UTSW	4	137,879,087 (GRCm39)	missense	possibly damaging	0.76
R1116:Eif4g3	UTSW	4	137,819,086 (GRCm39)	critical splice donor site	probably null
R1145:Eif4g3	UTSW	4	137,893,129 (GRCm39)	missense	probably damaging	1.00
R1145:Eif4g3	UTSW	4	137,893,129 (GRCm39)	missense	probably damaging	1.00
R1192:Eif4g3	UTSW	4	137,898,497 (GRCm39)	missense	probably damaging	1.00
R1401:Eif4g3	UTSW	4	137,933,395 (GRCm39)	missense	probably damaging	0.99
R1535:Eif4g3	UTSW	4	137,824,613 (GRCm39)	missense	probably damaging	1.00
R1571:Eif4g3	UTSW	4	137,847,719 (GRCm39)	missense	probably damaging	1.00
R1576:Eif4g3	UTSW	4	137,824,181 (GRCm39)	missense	probably damaging	0.99
R1607:Eif4g3	UTSW	4	137,853,874 (GRCm39)	missense	probably benign	0.00
R1618:Eif4g3	UTSW	4	137,933,369 (GRCm39)	missense	probably damaging	1.00
R1793:Eif4g3	UTSW	4	137,898,442 (GRCm39)	missense	probably damaging	1.00
R1823:Eif4g3	UTSW	4	137,907,802 (GRCm39)	missense	probably benign	0.37
R1857:Eif4g3	UTSW	4	137,903,187 (GRCm39)	missense	possibly damaging	0.67
R1907:Eif4g3	UTSW	4	137,885,726 (GRCm39)	missense	probably damaging	1.00
R2041:Eif4g3	UTSW	4	137,832,617 (GRCm39)	splice site	probably benign
R2106:Eif4g3	UTSW	4	137,810,230 (GRCm39)	start gained	probably benign
R2124:Eif4g3	UTSW	4	137,912,053 (GRCm39)	missense	probably damaging	1.00
R2301:Eif4g3	UTSW	4	137,899,970 (GRCm39)	missense	probably damaging	1.00
R2519:Eif4g3	UTSW	4	137,824,629 (GRCm39)	missense	probably benign	0.37
R3033:Eif4g3	UTSW	4	137,830,721 (GRCm39)	missense	probably damaging	1.00
R3870:Eif4g3	UTSW	4	137,824,211 (GRCm39)	missense	probably damaging	0.98
R4542:Eif4g3	UTSW	4	137,930,728 (GRCm39)	missense	probably damaging	0.99
R4582:Eif4g3	UTSW	4	137,898,556 (GRCm39)	missense	probably damaging	1.00
R4607:Eif4g3	UTSW	4	137,853,769 (GRCm39)	missense	probably benign	0.03
R4608:Eif4g3	UTSW	4	137,853,769 (GRCm39)	missense	probably benign	0.03
R4658:Eif4g3	UTSW	4	137,933,443 (GRCm39)	missense	probably damaging	1.00
R4736:Eif4g3	UTSW	4	137,925,408 (GRCm39)	missense	probably benign	0.01
R4739:Eif4g3	UTSW	4	137,925,408 (GRCm39)	missense	probably benign	0.01
R4739:Eif4g3	UTSW	4	137,910,510 (GRCm39)	missense	possibly damaging	0.79
R4740:Eif4g3	UTSW	4	137,925,408 (GRCm39)	missense	probably benign	0.01
R4760:Eif4g3	UTSW	4	137,811,629 (GRCm39)	missense	possibly damaging	0.46
R4826:Eif4g3	UTSW	4	137,905,256 (GRCm39)	missense	possibly damaging	0.95
R4941:Eif4g3	UTSW	4	137,897,876 (GRCm39)	missense	probably damaging	1.00
R5040:Eif4g3	UTSW	4	137,824,200 (GRCm39)	missense	probably damaging	0.99
R5070:Eif4g3	UTSW	4	137,873,610 (GRCm39)	missense	probably benign	0.00
R5155:Eif4g3	UTSW	4	137,854,054 (GRCm39)	missense	probably benign	0.36
R5226:Eif4g3	UTSW	4	137,824,105 (GRCm39)	missense	possibly damaging	0.93
R5229:Eif4g3	UTSW	4	137,824,105 (GRCm39)	missense	possibly damaging	0.93
R5303:Eif4g3	UTSW	4	137,853,873 (GRCm39)	missense	probably benign	0.04
R5369:Eif4g3	UTSW	4	137,910,645 (GRCm39)	missense	possibly damaging	0.87
R5394:Eif4g3	UTSW	4	137,830,709 (GRCm39)	splice site	probably null
R5665:Eif4g3	UTSW	4	137,853,900 (GRCm39)	missense	probably benign	0.03
R5678:Eif4g3	UTSW	4	137,879,053 (GRCm39)	missense	probably damaging	0.99
R5695:Eif4g3	UTSW	4	137,890,744 (GRCm39)	splice site	probably null
R5704:Eif4g3	UTSW	4	137,918,003 (GRCm39)	missense	probably damaging	1.00
R5924:Eif4g3	UTSW	4	137,929,237 (GRCm39)	missense	probably damaging	1.00
R6214:Eif4g3	UTSW	4	137,785,314 (GRCm39)	missense	probably damaging	0.99
R6278:Eif4g3	UTSW	4	137,915,394 (GRCm39)	missense	possibly damaging	0.82
R6519:Eif4g3	UTSW	4	137,721,319 (GRCm39)	missense	probably benign
R6659:Eif4g3	UTSW	4	137,905,243 (GRCm39)	missense	probably damaging	1.00
R6720:Eif4g3	UTSW	4	137,903,143 (GRCm39)	splice site	probably null
R6812:Eif4g3	UTSW	4	137,830,687 (GRCm39)	missense	probably damaging	1.00
R6922:Eif4g3	UTSW	4	137,824,646 (GRCm39)	missense	probably damaging	1.00
R7175:Eif4g3	UTSW	4	137,853,526 (GRCm39)	missense	probably damaging	1.00
R7176:Eif4g3	UTSW	4	137,898,497 (GRCm39)	missense	probably damaging	1.00
R7598:Eif4g3	UTSW	4	137,921,435 (GRCm39)	missense	probably benign	0.02
R7618:Eif4g3	UTSW	4	137,898,429 (GRCm39)	missense	probably damaging	1.00
R7805:Eif4g3	UTSW	4	137,873,665 (GRCm39)	missense	probably benign	0.00
R7935:Eif4g3	UTSW	4	137,824,082 (GRCm39)	missense	probably damaging	1.00
R7983:Eif4g3	UTSW	4	137,878,904 (GRCm39)	missense	probably benign	0.00
R8261:Eif4g3	UTSW	4	137,898,429 (GRCm39)	missense	possibly damaging	0.46
R8371:Eif4g3	UTSW	4	137,824,156 (GRCm39)	missense	probably damaging	1.00
R8499:Eif4g3	UTSW	4	137,893,239 (GRCm39)	missense	probably damaging	1.00
R8670:Eif4g3	UTSW	4	137,885,823 (GRCm39)	critical splice donor site	probably null
R8672:Eif4g3	UTSW	4	137,853,823 (GRCm39)	missense	possibly damaging	0.75
R8744:Eif4g3	UTSW	4	137,721,372 (GRCm39)	small deletion	probably benign
R8767:Eif4g3	UTSW	4	137,930,779 (GRCm39)	missense	probably damaging	0.99
R8771:Eif4g3	UTSW	4	137,907,848 (GRCm39)	nonsense	probably null
R8989:Eif4g3	UTSW	4	137,912,059 (GRCm39)	missense	probably damaging	1.00
R9292:Eif4g3	UTSW	4	137,921,382 (GRCm39)	missense	possibly damaging	0.82
R9294:Eif4g3	UTSW	4	137,917,968 (GRCm39)	missense	probably damaging	0.98
R9607:Eif4g3	UTSW	4	137,893,045 (GRCm39)	missense	probably benign	0.28
R9617:Eif4g3	UTSW	4	137,824,190 (GRCm39)	missense	probably damaging	0.99
RF008:Eif4g3	UTSW	4	137,903,235 (GRCm39)	missense	probably damaging	0.98
X0067:Eif4g3	UTSW	4	137,890,930 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCCCATTGAACTGATATGTGCTCAG -3'
(R):5'- ATAGCATCCAGGTCCCTCAG -3'

Sequencing Primer
(F):5'- GTGCTCAGATATCTCAGATACTAGGC -3'
(R):5'- TGATTCCTAGTCATCACTGTCG -3'

Posted On

2016-03-01