Mutagenetix > Incidental Mutation

Incidental Mutation 'R4839:Vps13d'

371612

Institutional Source

Beutler Lab

Gene Symbol

Vps13d

Ensembl Gene

ENSMUSG00000020220

Gene Name

vacuolar protein sorting 13D

Synonyms

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4839 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144699192-144921575 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 144812000 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 3305 (T3305A)

Ref Sequence

ENSEMBL: ENSMUSP00000020441 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020441] [ENSMUST00000036579] [ENSMUST00000130704]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000020441
AA Change: T3305A

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000020441
Gene: ENSMUSG00000020220
AA Change: T3305A

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	118	1.8e-37	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
coiled coil region	665	685	N/A	INTRINSIC
low complexity region	765	781	N/A	INTRINSIC
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2866	2884	N/A	INTRINSIC
low complexity region	2973	2983	N/A	INTRINSIC
Pfam:DUF1162	3246	3530	1.1e-110	PFAM
low complexity region	3797	3810	N/A	INTRINSIC
low complexity region	3913	3921	N/A	INTRINSIC
low complexity region	4119	4132	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000036579
AA Change: T3336A

SMART Domains

Protein: ENSMUSP00000043240
Gene: ENSMUSG00000020220
AA Change: T3336A

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	116	3.5e-35	PFAM
Pfam:VPS13	131	353	9.6e-57	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
Pfam:VPS13_mid_rpt	608	896	4.3e-35	PFAM
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2891	2909	N/A	INTRINSIC
low complexity region	2998	3008	N/A	INTRINSIC
Pfam:SHR-BD	3271	3555	4.2e-86	PFAM
low complexity region	3822	3835	N/A	INTRINSIC
low complexity region	3938	3946	N/A	INTRINSIC
Pfam:VPS13_C	3978	4126	4.8e-24	PFAM
low complexity region	4144	4157	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000130704
AA Change: T221A

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000118699
Gene: ENSMUSG00000020220
AA Change: T221A

Domain	Start	End	E-Value	Type
Pfam:DUF1162	162	446	1.4e-111	PFAM
low complexity region	713	726	N/A	INTRINSIC
low complexity region	829	837	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000185113
AA Change: T2156A

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	T	A	17: 9,226,845 (GRCm39)	S467T	probably benign	Het
2310030G06Rik	A	T	9: 50,652,023 (GRCm39)	Y68*	probably null	Het
Abat	T	G	16: 8,401,512 (GRCm39)		probably benign	Het
Abca2	C	T	2: 25,330,921 (GRCm39)	S1203F	probably damaging	Het
Adam22	G	A	5: 8,186,813 (GRCm39)	P436L	probably damaging	Het
Ak3	A	C	19: 29,025,132 (GRCm39)	L33R	probably damaging	Het
Arhgap11a	A	T	2: 113,672,374 (GRCm39)	I198K	probably damaging	Het
Arl14epl	A	T	18: 47,065,544 (GRCm39)	K103M	possibly damaging	Het
Atg16l1	T	A	1: 87,693,896 (GRCm39)	N65K	probably damaging	Het
Btnl2	G	A	17: 34,584,260 (GRCm39)	W394*	probably null	Het
Cacna1e	C	A	1: 154,296,804 (GRCm39)	R1687L	probably damaging	Het
Cacna1g	G	T	11: 94,350,433 (GRCm39)	R471S	probably benign	Het
Cdh13	A	T	8: 119,578,587 (GRCm39)	R205*	probably null	Het
Cdkl3	A	T	11: 51,895,861 (GRCm39)	Y36F	probably damaging	Het
Cep290	T	A	10: 100,344,648 (GRCm39)	N488K	probably damaging	Het
Cep350	A	T	1: 155,804,240 (GRCm39)	C948S	probably benign	Het
Cep70	A	G	9: 99,178,138 (GRCm39)	K446R	probably benign	Het
Cfap70	T	C	14: 20,475,597 (GRCm39)	T375A	probably damaging	Het
Cfhr1	A	G	1: 139,487,871 (GRCm39)	L9S	probably damaging	Het
Chrm4	T	A	2: 91,757,952 (GRCm39)	M120K	probably damaging	Het
Col1a1	A	G	11: 94,840,921 (GRCm39)		probably null	Het
Col3a1	T	C	1: 45,362,963 (GRCm39)		probably null	Het
Csrnp3	A	T	2: 65,852,375 (GRCm39)	R256*	probably null	Het
Dbf4	T	A	5: 8,458,263 (GRCm39)	K190*	probably null	Het
Dnah10	A	T	5: 124,850,196 (GRCm39)	I1573F	probably damaging	Het
Dock8	A	G	19: 25,146,858 (GRCm39)	I1446V	probably benign	Het
Dst	G	T	1: 34,229,943 (GRCm39)	R2187M	probably damaging	Het
Ece2	C	T	16: 20,449,918 (GRCm39)	R257C	probably damaging	Het
Espn	A	G	4: 152,222,961 (GRCm39)	Y306H	probably damaging	Het
Fkbpl	G	A	17: 34,864,839 (GRCm39)	M202I	probably benign	Het
Fn1	A	G	1: 71,681,242 (GRCm39)	L362P	probably damaging	Het
Ganc	A	C	2: 120,290,304 (GRCm39)	R880S	probably benign	Het
Gucy1b2	A	T	14: 62,685,695 (GRCm39)	L90Q	probably damaging	Het
Hcn1	T	A	13: 118,062,246 (GRCm39)	I504N	unknown	Het
Hdac2	C	A	10: 36,873,462 (GRCm39)	T352K	probably benign	Het
Hip1	T	C	5: 135,455,172 (GRCm39)		probably null	Het
Hspa13	G	A	16: 75,562,169 (GRCm39)	S10L	probably damaging	Het
Ipo5	T	C	14: 121,157,450 (GRCm39)	I96T	probably benign	Het
Isl1	T	C	13: 116,438,220 (GRCm39)	Y264C	probably damaging	Het
Izumo1	T	C	7: 45,275,657 (GRCm39)	I322T	probably benign	Het
Kars1	A	C	8: 112,729,158 (GRCm39)	V183G	possibly damaging	Het
Lrrc15	C	A	16: 30,093,086 (GRCm39)	M84I	probably benign	Het
Magi1	A	T	6: 93,671,177 (GRCm39)	V796E	probably damaging	Het
Matn4	G	T	2: 164,242,896 (GRCm39)	D67E	probably benign	Het
Mertk	A	G	2: 128,624,496 (GRCm39)	M636V	probably damaging	Het
Mettl27	C	A	5: 134,963,266 (GRCm39)	P126T	probably damaging	Het
Mfrp	T	A	9: 44,013,432 (GRCm39)	H52Q	possibly damaging	Het
Mrgprb8	T	G	7: 48,038,656 (GRCm39)	I109R	probably benign	Het
Mroh2a	G	A	1: 88,165,666 (GRCm39)	G512S	probably damaging	Het
Ncoa7	T	A	10: 30,598,655 (GRCm39)	R89S	possibly damaging	Het
Nfkb2	G	T	19: 46,296,006 (GRCm39)	E170D	probably benign	Het
Nin	G	T	12: 70,137,325 (GRCm39)	H84N	possibly damaging	Het
Nwd2	A	T	5: 63,962,893 (GRCm39)	I826F	possibly damaging	Het
Or10d1	A	G	9: 39,484,441 (GRCm39)	M38T	probably benign	Het
Or14c43	T	C	7: 86,115,117 (GRCm39)	F166S	probably damaging	Het
Or1j11	G	A	2: 36,312,012 (GRCm39)	V201I	probably benign	Het
Or4l15	C	T	14: 50,197,646 (GRCm39)	M294I	probably benign	Het
Or52a5b	T	C	7: 103,416,961 (GRCm39)	I214M	possibly damaging	Het
Or5k3	A	G	16: 58,969,393 (GRCm39)	Y60C	probably damaging	Het
Or5p73	T	C	7: 108,064,938 (GRCm39)	S136P	probably benign	Het
Otof	C	T	5: 30,576,748 (GRCm39)	R168H	probably damaging	Het
Pcdha6	T	C	18: 37,101,485 (GRCm39)	V226A	possibly damaging	Het
Pggt1b	T	C	18: 46,391,166 (GRCm39)	I200V	possibly damaging	Het
Ppat	G	A	5: 77,098,811 (GRCm39)	Q41*	probably null	Het
Ppfibp2	A	T	7: 107,342,192 (GRCm39)	H27L	probably damaging	Het
Rbm27	T	A	18: 42,460,510 (GRCm39)	I775N	probably damaging	Het
Rnf40	C	T	7: 127,191,812 (GRCm39)	R349*	probably null	Het
Rtp3	A	T	9: 110,818,544 (GRCm39)	W46R	probably damaging	Het
Sass6	C	T	3: 116,403,949 (GRCm39)	R196W	probably damaging	Het
Sel1l	A	T	12: 91,799,932 (GRCm39)	D131E	probably benign	Het
Slain2	T	C	5: 73,106,066 (GRCm39)	S234P	probably damaging	Het
Slc47a1	C	T	11: 61,264,176 (GRCm39)		probably null	Het
Sp140	CAGAAGAAG	CAGAAG	1: 85,538,529 (GRCm39)		probably benign	Het
Spata31e2	T	C	1: 26,724,440 (GRCm39)	T247A	probably benign	Het
Spef2	A	T	15: 9,713,264 (GRCm39)	Y369*	probably null	Het
Spred2	T	A	11: 19,948,233 (GRCm39)	M76K	possibly damaging	Het
Svs5	T	A	2: 164,078,806 (GRCm39)	D367V	probably benign	Het
Tasor2	G	A	13: 3,634,807 (GRCm39)	P667S	probably damaging	Het
Tefm	A	T	11: 80,027,947 (GRCm39)	D270E	probably benign	Het
Tfcp2l1	C	A	1: 118,597,194 (GRCm39)	P425H	probably benign	Het
Thbd	A	T	2: 148,248,591 (GRCm39)	C426S	probably damaging	Het
Timm10b	T	A	7: 105,333,219 (GRCm39)	D968E	probably damaging	Het
Tle2	C	T	10: 81,413,518 (GRCm39)	T119I	probably damaging	Het
Tnik	T	A	3: 28,650,224 (GRCm39)	Y446N	possibly damaging	Het
Tpr	T	A	1: 150,324,948 (GRCm39)	L2400*	probably null	Het
Trrap	T	C	5: 144,782,402 (GRCm39)	F3328S	probably damaging	Het
Vldlr	G	A	19: 27,215,465 (GRCm39)	C154Y	probably damaging	Het
Vmn1r79	T	G	7: 11,910,361 (GRCm39)	M81R	probably benign	Het
Wdr62	T	C	7: 29,970,111 (GRCm39)	E232G	probably damaging	Het
Wdr62	T	A	7: 29,940,890 (GRCm39)	I843F	probably benign	Het
Zfp747l1	T	C	7: 126,984,179 (GRCm39)	S308G	probably benign	Het
Zfp873	T	A	10: 81,896,353 (GRCm39)	H361Q	probably damaging	Het
Zfp949	A	C	9: 88,452,047 (GRCm39)	H539P	probably damaging	Het

Other mutations in Vps13d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Vps13d	APN	4	144,895,110 (GRCm39)	missense	probably damaging	0.98
IGL00484:Vps13d	APN	4	144,853,145 (GRCm39)	missense	probably benign	0.04
IGL00591:Vps13d	APN	4	144,917,129 (GRCm39)	missense	possibly damaging	0.95
IGL00816:Vps13d	APN	4	144,882,564 (GRCm39)	missense	probably benign	0.00
IGL00835:Vps13d	APN	4	144,887,222 (GRCm39)	missense	probably damaging	0.97
IGL00847:Vps13d	APN	4	144,811,978 (GRCm39)	missense	probably benign	0.26
IGL01084:Vps13d	APN	4	144,881,525 (GRCm39)	missense	probably benign	0.00
IGL01116:Vps13d	APN	4	144,699,320 (GRCm39)	unclassified	probably benign
IGL01150:Vps13d	APN	4	144,875,845 (GRCm39)	missense	probably benign
IGL01329:Vps13d	APN	4	144,882,776 (GRCm39)	missense	possibly damaging	0.69
IGL01338:Vps13d	APN	4	144,814,892 (GRCm39)	missense	probably damaging	1.00
IGL01583:Vps13d	APN	4	144,771,658 (GRCm39)	missense	probably damaging	1.00
IGL01598:Vps13d	APN	4	144,743,471 (GRCm39)	missense	probably benign	0.21
IGL01620:Vps13d	APN	4	144,821,437 (GRCm39)	missense	possibly damaging	0.70
IGL01636:Vps13d	APN	4	144,801,618 (GRCm39)	missense	probably damaging	1.00
IGL01723:Vps13d	APN	4	144,899,715 (GRCm39)	missense	possibly damaging	0.84
IGL01895:Vps13d	APN	4	144,882,836 (GRCm39)	missense	possibly damaging	0.57
IGL01981:Vps13d	APN	4	144,813,317 (GRCm39)	missense	probably damaging	0.99
IGL02192:Vps13d	APN	4	144,875,428 (GRCm39)	missense	probably benign	0.02
IGL02197:Vps13d	APN	4	144,854,879 (GRCm39)	missense	probably benign	0.01
IGL02209:Vps13d	APN	4	144,882,671 (GRCm39)	missense	probably damaging	0.97
IGL02219:Vps13d	APN	4	144,894,716 (GRCm39)	missense	probably benign	0.00
IGL02377:Vps13d	APN	4	144,882,934 (GRCm39)	missense	probably damaging	1.00
IGL02404:Vps13d	APN	4	144,875,305 (GRCm39)	missense	probably damaging	1.00
IGL02552:Vps13d	APN	4	144,899,707 (GRCm39)	missense	possibly damaging	0.46
IGL02651:Vps13d	APN	4	144,891,129 (GRCm39)	missense	probably benign	0.02
IGL02708:Vps13d	APN	4	144,854,850 (GRCm39)	missense	probably benign	0.12
IGL02811:Vps13d	APN	4	144,858,335 (GRCm39)	missense	possibly damaging	0.55
IGL02821:Vps13d	APN	4	144,875,332 (GRCm39)	missense	probably damaging	0.98
IGL02838:Vps13d	APN	4	144,801,595 (GRCm39)	missense	probably benign	0.31
IGL02968:Vps13d	APN	4	144,849,068 (GRCm39)	missense	probably benign	0.32
IGL03176:Vps13d	APN	4	144,801,533 (GRCm39)	missense	probably benign	0.16
IGL03352:Vps13d	APN	4	144,894,072 (GRCm39)	missense	possibly damaging	0.49
IGL03374:Vps13d	APN	4	144,835,145 (GRCm39)	missense	possibly damaging	0.70
IGL03375:Vps13d	APN	4	144,818,517 (GRCm39)	missense	probably damaging	1.00
IGL03383:Vps13d	APN	4	144,894,889 (GRCm39)	critical splice acceptor site	probably null
IGL03411:Vps13d	APN	4	144,875,894 (GRCm39)	missense	probably damaging	1.00
broken	UTSW	4	144,813,305 (GRCm39)	missense
demotion	UTSW	4	144,865,183 (GRCm39)	missense
BB008:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
BB018:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
PIT4283001:Vps13d	UTSW	4	144,835,158 (GRCm39)	missense
PIT4434001:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0076:Vps13d	UTSW	4	144,891,264 (GRCm39)	splice site	probably benign
R0211:Vps13d	UTSW	4	144,841,348 (GRCm39)	missense	probably benign	0.08
R0219:Vps13d	UTSW	4	144,832,479 (GRCm39)	missense	probably benign	0.01
R0284:Vps13d	UTSW	4	144,871,372 (GRCm39)	missense	probably benign	0.01
R0345:Vps13d	UTSW	4	144,844,195 (GRCm39)	missense	possibly damaging	0.81
R0400:Vps13d	UTSW	4	144,792,397 (GRCm39)	missense	probably benign	0.00
R0417:Vps13d	UTSW	4	144,703,130 (GRCm39)	missense	probably benign	0.19
R0538:Vps13d	UTSW	4	144,771,665 (GRCm39)	missense	probably damaging	1.00
R0560:Vps13d	UTSW	4	144,780,760 (GRCm39)	missense	probably damaging	1.00
R0627:Vps13d	UTSW	4	144,813,754 (GRCm39)	missense	probably damaging	1.00
R0707:Vps13d	UTSW	4	144,882,502 (GRCm39)	missense	probably damaging	1.00
R0782:Vps13d	UTSW	4	144,853,195 (GRCm39)	splice site	probably benign
R0925:Vps13d	UTSW	4	144,883,121 (GRCm39)	missense	probably damaging	1.00
R0993:Vps13d	UTSW	4	144,844,262 (GRCm39)	nonsense	probably null
R1135:Vps13d	UTSW	4	144,882,159 (GRCm39)	missense	probably benign	0.01
R1165:Vps13d	UTSW	4	144,853,041 (GRCm39)	missense	probably benign
R1263:Vps13d	UTSW	4	144,896,918 (GRCm39)	missense	probably benign	0.01
R1397:Vps13d	UTSW	4	144,867,904 (GRCm39)	missense	probably damaging	1.00
R1398:Vps13d	UTSW	4	144,826,553 (GRCm39)	missense	probably null
R1521:Vps13d	UTSW	4	144,832,431 (GRCm39)	missense	probably benign	0.00
R1522:Vps13d	UTSW	4	144,824,742 (GRCm39)	splice site	probably null
R1725:Vps13d	UTSW	4	144,869,830 (GRCm39)	missense	possibly damaging	0.90
R1759:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1826:Vps13d	UTSW	4	144,881,573 (GRCm39)	missense	probably damaging	0.96
R1900:Vps13d	UTSW	4	144,853,176 (GRCm39)	missense	probably benign	0.23
R1943:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1955:Vps13d	UTSW	4	144,882,713 (GRCm39)	missense	probably damaging	1.00
R2008:Vps13d	UTSW	4	144,881,813 (GRCm39)	missense	probably benign	0.00
R2013:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2014:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2038:Vps13d	UTSW	4	144,907,685 (GRCm39)	critical splice donor site	probably null
R2108:Vps13d	UTSW	4	144,801,617 (GRCm39)	missense	probably damaging	0.99
R2130:Vps13d	UTSW	4	144,882,671 (GRCm39)	missense	probably benign	0.17
R2134:Vps13d	UTSW	4	144,874,909 (GRCm39)	missense	probably benign	0.00
R2168:Vps13d	UTSW	4	144,813,893 (GRCm39)	splice site	probably benign
R2220:Vps13d	UTSW	4	144,904,890 (GRCm39)	missense	probably damaging	1.00
R2240:Vps13d	UTSW	4	144,837,465 (GRCm39)	missense	possibly damaging	0.70
R2332:Vps13d	UTSW	4	144,875,256 (GRCm39)	missense	probably benign
R2357:Vps13d	UTSW	4	144,801,547 (GRCm39)	frame shift	probably null
R2365:Vps13d	UTSW	4	144,813,894 (GRCm39)	splice site	probably benign
R2571:Vps13d	UTSW	4	144,875,706 (GRCm39)	missense	probably benign	0.20
R3149:Vps13d	UTSW	4	144,853,147 (GRCm39)	missense	possibly damaging	0.70
R3150:Vps13d	UTSW	4	144,813,360 (GRCm39)	missense	probably damaging	0.98
R3547:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably damaging	0.99
R3716:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3718:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3725:Vps13d	UTSW	4	144,842,218 (GRCm39)	splice site	probably benign
R3794:Vps13d	UTSW	4	144,812,007 (GRCm39)	splice site	probably benign
R3875:Vps13d	UTSW	4	144,917,114 (GRCm39)	missense	probably damaging	1.00
R3948:Vps13d	UTSW	4	144,867,910 (GRCm39)	missense	probably damaging	1.00
R3953:Vps13d	UTSW	4	144,875,450 (GRCm39)	missense	probably damaging	1.00
R4021:Vps13d	UTSW	4	144,801,631 (GRCm39)	missense	possibly damaging	0.90
R4323:Vps13d	UTSW	4	144,879,348 (GRCm39)	missense	probably benign	0.28
R4346:Vps13d	UTSW	4	144,799,099 (GRCm39)	intron	probably benign
R4509:Vps13d	UTSW	4	144,789,172 (GRCm39)	missense	probably damaging	1.00
R4613:Vps13d	UTSW	4	144,858,225 (GRCm39)	missense	possibly damaging	0.95
R4657:Vps13d	UTSW	4	144,801,412 (GRCm39)	missense	probably damaging	1.00
R4680:Vps13d	UTSW	4	144,835,080 (GRCm39)	missense	possibly damaging	0.94
R4688:Vps13d	UTSW	4	144,904,782 (GRCm39)	missense	probably benign
R4797:Vps13d	UTSW	4	144,780,725 (GRCm39)	missense	probably damaging	1.00
R4798:Vps13d	UTSW	4	144,904,626 (GRCm39)	missense	probably damaging	0.98
R4817:Vps13d	UTSW	4	144,795,735 (GRCm39)	missense	probably damaging	1.00
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4869:Vps13d	UTSW	4	144,854,612 (GRCm39)	missense	probably damaging	1.00
R4904:Vps13d	UTSW	4	144,882,015 (GRCm39)	missense	probably damaging	1.00
R4912:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R4916:Vps13d	UTSW	4	144,709,963 (GRCm39)	missense	probably damaging	1.00
R4976:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5029:Vps13d	UTSW	4	144,882,852 (GRCm39)	missense	probably benign	0.02
R5049:Vps13d	UTSW	4	144,813,336 (GRCm39)	missense	probably damaging	1.00
R5077:Vps13d	UTSW	4	144,814,811 (GRCm39)	missense	probably damaging	0.98
R5119:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5227:Vps13d	UTSW	4	144,907,777 (GRCm39)	splice site	probably null
R5291:Vps13d	UTSW	4	144,789,139 (GRCm39)	missense	probably damaging	0.99
R5344:Vps13d	UTSW	4	144,904,904 (GRCm39)	missense	probably damaging	0.98
R5348:Vps13d	UTSW	4	144,792,459 (GRCm39)	missense	probably damaging	0.99
R5478:Vps13d	UTSW	4	144,894,120 (GRCm39)	missense	probably damaging	0.99
R5632:Vps13d	UTSW	4	144,801,452 (GRCm39)	missense	probably damaging	0.99
R5642:Vps13d	UTSW	4	144,896,872 (GRCm39)	missense	possibly damaging	0.66
R5712:Vps13d	UTSW	4	144,813,743 (GRCm39)	missense	probably benign	0.07
R5747:Vps13d	UTSW	4	144,894,853 (GRCm39)	missense	probably benign	0.00
R5752:Vps13d	UTSW	4	144,875,540 (GRCm39)	missense	probably benign	0.06
R5804:Vps13d	UTSW	4	144,826,640 (GRCm39)	missense	probably benign	0.03
R5917:Vps13d	UTSW	4	144,826,580 (GRCm39)	missense	probably damaging	0.96
R5932:Vps13d	UTSW	4	144,771,611 (GRCm39)	missense	possibly damaging	0.71
R5940:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably benign	0.09
R5978:Vps13d	UTSW	4	144,849,181 (GRCm39)	missense	probably benign
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6143:Vps13d	UTSW	4	144,875,135 (GRCm39)	missense	possibly damaging	0.95
R6174:Vps13d	UTSW	4	144,701,763 (GRCm39)	nonsense	probably null
R6191:Vps13d	UTSW	4	144,875,918 (GRCm39)	missense	probably damaging	1.00
R6198:Vps13d	UTSW	4	144,875,560 (GRCm39)	missense	probably benign	0.28
R6374:Vps13d	UTSW	4	144,849,251 (GRCm39)	missense	probably damaging	1.00
R6379:Vps13d	UTSW	4	144,814,828 (GRCm39)	missense	probably benign
R6388:Vps13d	UTSW	4	144,882,144 (GRCm39)	missense	probably benign	0.06
R6418:Vps13d	UTSW	4	144,818,850 (GRCm39)	missense	probably damaging	0.98
R6466:Vps13d	UTSW	4	144,784,065 (GRCm39)	missense	possibly damaging	0.47
R6602:Vps13d	UTSW	4	144,830,234 (GRCm39)	intron	probably benign
R6604:Vps13d	UTSW	4	144,907,694 (GRCm39)	missense	probably damaging	1.00
R7051:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7052:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7103:Vps13d	UTSW	4	144,842,062 (GRCm39)	missense
R7231:Vps13d	UTSW	4	144,784,032 (GRCm39)	missense
R7246:Vps13d	UTSW	4	144,882,620 (GRCm39)	missense
R7339:Vps13d	UTSW	4	144,847,938 (GRCm39)	missense
R7409:Vps13d	UTSW	4	144,867,824 (GRCm39)	missense
R7419:Vps13d	UTSW	4	144,842,073 (GRCm39)	missense
R7424:Vps13d	UTSW	4	144,875,317 (GRCm39)	missense
R7439:Vps13d	UTSW	4	144,832,426 (GRCm39)	missense
R7440:Vps13d	UTSW	4	144,854,981 (GRCm39)	missense
R7528:Vps13d	UTSW	4	144,818,492 (GRCm39)	missense
R7547:Vps13d	UTSW	4	144,784,108 (GRCm39)	missense
R7558:Vps13d	UTSW	4	144,881,150 (GRCm39)	missense
R7699:Vps13d	UTSW	4	144,811,975 (GRCm39)	missense
R7729:Vps13d	UTSW	4	144,801,622 (GRCm39)	missense
R7789:Vps13d	UTSW	4	144,826,635 (GRCm39)	missense
R7813:Vps13d	UTSW	4	144,904,633 (GRCm39)	nonsense	probably null
R7834:Vps13d	UTSW	4	144,835,143 (GRCm39)	missense
R7840:Vps13d	UTSW	4	144,830,246 (GRCm39)	missense
R7880:Vps13d	UTSW	4	144,907,684 (GRCm39)	critical splice donor site	probably null
R7912:Vps13d	UTSW	4	144,899,697 (GRCm39)	missense
R7915:Vps13d	UTSW	4	144,813,389 (GRCm39)	missense
R7931:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
R8021:Vps13d	UTSW	4	144,875,245 (GRCm39)	missense
R8048:Vps13d	UTSW	4	144,882,137 (GRCm39)	missense
R8057:Vps13d	UTSW	4	144,701,753 (GRCm39)	missense
R8063:Vps13d	UTSW	4	144,841,327 (GRCm39)	missense
R8131:Vps13d	UTSW	4	144,882,707 (GRCm39)	missense
R8190:Vps13d	UTSW	4	144,879,321 (GRCm39)	missense
R8226:Vps13d	UTSW	4	144,875,860 (GRCm39)	missense
R8241:Vps13d	UTSW	4	144,875,047 (GRCm39)	missense
R8254:Vps13d	UTSW	4	144,709,882 (GRCm39)	splice site	probably benign
R8305:Vps13d	UTSW	4	144,818,858 (GRCm39)	missense
R8415:Vps13d	UTSW	4	144,818,549 (GRCm39)	missense
R8460:Vps13d	UTSW	4	144,897,009 (GRCm39)	intron	probably benign
R8487:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense	probably benign	0.11
R8543:Vps13d	UTSW	4	144,743,353 (GRCm39)	nonsense	probably null
R8679:Vps13d	UTSW	4	144,811,977 (GRCm39)	missense
R8716:Vps13d	UTSW	4	144,802,348 (GRCm39)	missense
R8749:Vps13d	UTSW	4	144,865,183 (GRCm39)	missense
R8772:Vps13d	UTSW	4	144,801,602 (GRCm39)	missense
R8788:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R8789:Vps13d	UTSW	4	144,795,743 (GRCm39)	missense
R8836:Vps13d	UTSW	4	144,882,648 (GRCm39)	missense
R8874:Vps13d	UTSW	4	144,881,772 (GRCm39)	missense
R8918:Vps13d	UTSW	4	144,772,873 (GRCm39)	missense
R9129:Vps13d	UTSW	4	144,898,249 (GRCm39)	missense
R9220:Vps13d	UTSW	4	144,783,058 (GRCm39)	missense
R9233:Vps13d	UTSW	4	144,879,344 (GRCm39)	missense
R9234:Vps13d	UTSW	4	144,875,792 (GRCm39)	missense
R9256:Vps13d	UTSW	4	144,882,374 (GRCm39)	missense
R9350:Vps13d	UTSW	4	144,882,333 (GRCm39)	missense
R9398:Vps13d	UTSW	4	144,896,956 (GRCm39)	nonsense	probably null
R9415:Vps13d	UTSW	4	144,796,527 (GRCm39)	missense
R9438:Vps13d	UTSW	4	144,858,314 (GRCm39)	missense
R9469:Vps13d	UTSW	4	144,780,691 (GRCm39)	missense
R9487:Vps13d	UTSW	4	144,807,869 (GRCm39)	critical splice donor site	probably null
R9524:Vps13d	UTSW	4	144,822,814 (GRCm39)	missense
R9616:Vps13d	UTSW	4	144,824,701 (GRCm39)	missense
R9655:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R9709:Vps13d	UTSW	4	144,875,915 (GRCm39)	missense
R9767:Vps13d	UTSW	4	144,879,306 (GRCm39)	missense
R9773:Vps13d	UTSW	4	144,818,619 (GRCm39)	missense
R9779:Vps13d	UTSW	4	144,798,972 (GRCm39)	missense
R9796:Vps13d	UTSW	4	144,854,505 (GRCm39)	critical splice donor site	probably null
X0021:Vps13d	UTSW	4	144,881,595 (GRCm39)	missense	probably damaging	0.99
Z1176:Vps13d	UTSW	4	144,833,637 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,904,866 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,881,478 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- CAGGCCAACATGCATAGCTG -3'
(R):5'- TGCTATGCTGTGGCTACTTC -3'

Sequencing Primer
(F):5'- GCATAGCTGATTCACTTATGGAGGAC -3'
(R):5'- CTACTTCTGGAGTTAATTGTCTTGC -3'

Posted On

2016-03-01