Mutagenetix > Incidental Mutation

Incidental Mutation 'R4840:Vmn2r60'

371729

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Gprc2a-rs3, Casr-rs3, EG637898

MMRRC Submission

042453-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.113) question?

Stock #

R4840 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42116471-42195776 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 42135861 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 166 (P166S)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166447
AA Change: P166S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: P166S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.1%

Validation Efficiency

97% (114/117)

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810459M11Rik	C	A	1: 86,046,444	T161K	probably benign	Het
Actn3	G	A	19: 4,864,511	R530W	probably damaging	Het
Alg11	C	T	8: 22,068,010	A404V	possibly damaging	Het
Alkbh8	T	A	9: 3,369,751	V340D	probably damaging	Het
Arhgef12	T	C	9: 42,975,068	H1166R	probably benign	Het
Aspm	T	A	1: 139,470,531	D978E	possibly damaging	Het
Bod1l	C	T	5: 41,818,472	G1833D	probably damaging	Het
Brd3	A	G	2: 27,449,239	V676A	possibly damaging	Het
Brip1	G	A	11: 86,146,183	T454I	possibly damaging	Het
C3ar1	T	C	6: 122,850,764	I165V	probably benign	Het
C87977	T	C	4: 144,208,574	K199R	probably damaging	Het
Camta1	T	A	4: 151,144,407	Q656L	probably benign	Het
Casp14	A	T	10: 78,713,344	L256*	probably null	Het
Cdh23	G	T	10: 60,419,777	H773Q	possibly damaging	Het
Chd1	G	A	17: 15,768,753	W1589*	probably null	Het
Chd1	G	T	17: 15,768,754	D1590Y	probably damaging	Het
Cldn19	A	G	4: 119,255,754	Q61R	probably damaging	Het
Cyp2a22	A	G	7: 26,932,524	S436P	probably benign	Het
E130218I03Rik	A	G	4: 134,245,276		probably benign	Het
Emc10	A	C	7: 44,492,627	V124G	probably damaging	Het
Enam	A	T	5: 88,503,026	D723V	probably benign	Het
Eps8	C	A	6: 137,527,130	Q158H	probably damaging	Het
Ercc6	G	A	14: 32,541,296	D486N	probably damaging	Het
Fasn	T	C	11: 120,813,059	E1485G	possibly damaging	Het
Fat2	T	C	11: 55,279,018	K2972E	probably benign	Het
Fbxw28	T	C	9: 109,339,534	K30R	probably null	Het
Flot2	T	A	11: 78,057,513	L164Q	probably damaging	Het
Fsip2	A	T	2: 82,949,395	I162L	probably benign	Het
Fsip2	A	T	2: 82,985,471	L3849F	probably benign	Het
Gabrb1	C	G	5: 71,700,811	P60R	probably damaging	Het
Galnt14	T	C	17: 73,504,898	R443G	probably benign	Het
Gas8	C	T	8: 123,531,014	T400M	probably benign	Het
Gfap	T	C	11: 102,894,388	Y254C	probably damaging	Het
Git2	A	G	5: 114,745,482	S396P	probably damaging	Het
Glb1l3	A	T	9: 26,829,053	M327K	probably benign	Het
Gm10715	A	C	9: 3,038,062		probably benign	Het
Gm10787	G	A	10: 77,022,007		noncoding transcript	Het
Gm1123	T	A	9: 99,018,569	D78V	probably damaging	Het
Gm27013	T	C	6: 130,678,116	T128A	probably benign	Het
Gm8979	T	C	7: 106,081,420		noncoding transcript	Het
Gpbp1	A	G	13: 111,440,630		probably null	Het
Gphn	G	A	12: 78,522,955		probably null	Het
Gpr157	A	G	4: 150,102,366	E317G	probably benign	Het
Gsdmc4	T	A	15: 63,893,747	M318L	probably benign	Het
Gtf2f2	A	T	14: 76,010,691	W19R	probably damaging	Het
Helb	A	G	10: 120,084,858	V1060A	probably benign	Het
Igfn1	C	T	1: 135,968,040	G1596D	probably benign	Het
Il1rl2	T	C	1: 40,327,387	I27T	possibly damaging	Het
Inpp5j	A	G	11: 3,499,676	V702A	probably damaging	Het
Kmt2d	A	C	15: 98,861,894	V1161G	unknown	Het
Krtap1-3	T	G	11: 99,590,889	Y144S	possibly damaging	Het
Layn	C	T	9: 51,057,382	V354M	probably damaging	Het
Lrba	G	A	3: 86,619,509		probably null	Het
Lrp8	T	A	4: 107,870,037	L893Q	possibly damaging	Het
Mrps9	T	A	1: 42,898,415		probably benign	Het
Mug1	T	A	6: 121,885,854	M1387K	probably damaging	Het
Myo18b	A	C	5: 112,874,029	V499G	probably benign	Het
Nbea	T	C	3: 55,710,670	E2321G	probably benign	Het
Nrsn2	C	T	2: 152,369,632	V160I	probably benign	Het
Nup210	G	A	6: 91,031,668	Q510*	probably null	Het
Ofcc1	G	A	13: 40,015,388	T841I	probably damaging	Het
Olfr693	T	G	7: 106,678,123	D121A	probably damaging	Het
P3h3	G	T	6: 124,850,637	Q479K	possibly damaging	Het
Paqr9	T	A	9: 95,560,670	F238I	probably damaging	Het
Parp3	A	T	9: 106,473,109	L343H	probably damaging	Het
Pcdh18	A	C	3: 49,744,668	M1115R	probably damaging	Het
Pcdh8	A	G	14: 79,770,868	V85A	possibly damaging	Het
Pcdhb4	A	G	18: 37,308,399	N254S	possibly damaging	Het
Pld1	T	C	3: 28,076,551	V500A	probably benign	Het
Prkg2	A	T	5: 98,981,143	D311E	probably benign	Het
Prss42	T	C	9: 110,799,301	L171P	probably damaging	Het
Pth2	T	A	7: 45,181,343	L17H	probably damaging	Het
Reln	A	T	5: 22,018,846		probably null	Het
Rmi2	G	T	16: 10,839,837	V104L	probably damaging	Het
Rpusd4	T	A	9: 35,268,535	V108D	probably damaging	Het
Rufy4	C	A	1: 74,129,039	T82K	possibly damaging	Het
Scimp	G	A	11: 70,791,468	Q141*	probably null	Het
Sel1l2	T	C	2: 140,263,470	T267A	probably benign	Het
Sema5a	T	A	15: 32,550,254	S146R	possibly damaging	Het
Sh2d3c	T	C	2: 32,721,160	M1T	probably null	Het
Slc30a6	T	C	17: 74,405,721	L71P	probably damaging	Het
Srrm3	A	G	5: 135,854,595	Y224C	possibly damaging	Het
Tacr3	A	T	3: 134,854,854	T185S	possibly damaging	Het
Tas2r140	T	A	6: 133,055,565	T77S	probably benign	Het
Thsd7b	T	C	1: 129,595,844	V128A	probably benign	Het
Tnfrsf10b	T	G	14: 69,776,159	H179Q	probably damaging	Het
Tonsl	A	G	15: 76,633,209	V770A	probably benign	Het
Trim42	G	A	9: 97,362,929	P606L	probably benign	Het
Trim45	A	T	3: 100,925,488	T346S	possibly damaging	Het
Ttc28	C	A	5: 111,286,081	S2296Y	probably damaging	Het
Ttc41	C	T	10: 86,731,125	R552C	probably benign	Het
Tube1	A	G	10: 39,144,846	N243D	probably benign	Het
Tvp23b	G	T	11: 62,879,598		probably null	Het
Ubxn11	T	A	4: 134,109,608	I49N	probably damaging	Het
Usp17le	T	A	7: 104,769,770	E55V	probably benign	Het
Vmn2r114	A	T	17: 23,291,379	V709D	probably damaging	Het
Vmn2r23	C	T	6: 123,713,074	T303M	probably damaging	Het
Vmn2r83	A	G	10: 79,477,848	I97V	possibly damaging	Het
Vmn2r-ps159	G	C	4: 156,333,438		noncoding transcript	Het
Wbp2nl	A	T	15: 82,314,336	K358M	possibly damaging	Het
Xpo1	T	A	11: 23,278,183	I150N	probably damaging	Het
Zfp113	G	A	5: 138,145,425	L188F	probably damaging	Het
Zfp189	T	G	4: 49,529,984	S362R	probably damaging	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	42195154	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	42195466	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	42195586	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	42142296	nonsense	probably null
IGL03135:Vmn2r60	APN	7	42136594	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	42116547	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	42195140	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	42135561	splice site	probably benign
R0328:Vmn2r60	UTSW	7	42142320	splice site	probably benign
R0464:Vmn2r60	UTSW	7	42135831	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	42195445	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	42194941	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	42195771	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	42137052	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	42136713	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	42135855	nonsense	probably null
R1628:Vmn2r60	UTSW	7	42136406	nonsense	probably null
R1883:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	42195507	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	42136827	nonsense	probably null
R2847:Vmn2r60	UTSW	7	42136433	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	42140979	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	42135796	missense	probably benign
R2921:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	42116556	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	42136454	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	42195243	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R5173:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	42137024	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	42135730	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	42195625	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	42116508	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	42195423	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	42136976	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	42135621	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	42116471	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	42194886	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	42142292	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	42142242	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	42137063	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	42195112	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	42136742	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	42136402	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	42195207	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7527:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7528:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7764:Vmn2r60	UTSW	7	42195111	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	42195087	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	42141097	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	42142266	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	42141070	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	42195530	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	42195426	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	42136745	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	42142230	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	42141094	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	42136354	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	42142299	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	42136531	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	42194908	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	42135748	missense	probably benign
RF024:Vmn2r60	UTSW	7	42140939	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	42141114	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGGAACCCTGATCTTCTACCC -3'
(R):5'- TGAAAATGGACTTGCCCTGTAAAC -3'

Sequencing Primer
(F):5'- ACCCTGATCTTCTACCCAATATAAC -3'
(R):5'- GGACTTGCCCTGTAAACCCATTAC -3'

Posted On

2016-03-01