Incidental Mutation 'R4860:Dpep3'
ID 372422
Institutional Source Beutler Lab
Gene Symbol Dpep3
Ensembl Gene ENSMUSG00000031898
Gene Name dipeptidase 3
Synonyms MBD-3, 1700018F16Rik
MMRRC Submission 042471-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.066) question?
Stock # R4860 (G1)
Quality Score 181
Status Not validated
Chromosome 8
Chromosomal Location 105973513-105979429 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to G at 105976189 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Leucine at position 314 (I314L)
Ref Sequence ENSEMBL: ENSMUSP00000034371 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034371]
AlphaFold Q9DA79
Predicted Effect probably benign
Transcript: ENSMUST00000034371
AA Change: I314L

PolyPhen 2 Score 0.343 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000034371
Gene: ENSMUSG00000031898
AA Change: I314L

signal peptide 1 40 N/A INTRINSIC
Pfam:Peptidase_M19 83 404 1.2e-110 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212917
Meta Mutation Damage Score 0.0582 question?
Coding Region Coverage
  • 1x: 98.9%
  • 3x: 97.9%
  • 10x: 95.0%
  • 20x: 87.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a membrane-bound glycoprotein from the family of dipeptidases involved in hydrolytic metabolism of various dipeptides, including penem and carbapenem beta-lactam antibiotics. This gene is located on chromosome 16 in a cluster with another member of this family. Alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 92 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700030K09Rik T C 8: 72,455,423 (GRCm38) S466P possibly damaging Het
1700061G19Rik A G 17: 56,888,655 (GRCm38) N684S probably benign Het
4930435E12Rik A G 16: 38,828,145 (GRCm38) S201P probably damaging Het
Ablim1 T C 19: 57,079,866 (GRCm38) T267A probably damaging Het
Acap2 A T 16: 31,103,499 (GRCm38) L724Q possibly damaging Het
Adcy4 T C 14: 55,781,927 (GRCm38) T89A possibly damaging Het
Agrp T C 8: 105,567,368 (GRCm38) E41G probably benign Het
Akr1d1 G A 6: 37,564,491 (GRCm38) V308M probably damaging Het
Ap1m2 C T 9: 21,309,674 (GRCm38) R54Q probably benign Het
Arhgap45 T A 10: 80,027,066 (GRCm38) V692E probably damaging Het
Arid5b G T 10: 68,243,095 (GRCm38) N137K probably damaging Het
Arsi G A 18: 60,916,651 (GRCm38) G202E probably benign Het
BC048679 T C 7: 81,495,720 (GRCm38) N27D probably benign Het
Ccdc78 A G 17: 25,788,700 (GRCm38) N237S probably benign Het
Cd46 G A 1: 195,062,396 (GRCm38) L345F possibly damaging Het
Cngb3 A T 4: 19,425,569 (GRCm38) N459I possibly damaging Het
Ctnnd2 A G 15: 30,881,167 (GRCm38) E731G probably damaging Het
Ctsh A G 9: 90,054,548 (GRCm38) E26G probably benign Het
Cul1 G T 6: 47,517,146 (GRCm38) K464N probably benign Het
Cul1 T A 6: 47,517,191 (GRCm38) S479R probably damaging Het
Dcaf5 A T 12: 80,340,232 (GRCm38) D373E probably benign Het
Ddx58 G T 4: 40,210,000 (GRCm38) S644R probably damaging Het
Dhcr7 A G 7: 143,840,500 (GRCm38) Q126R probably benign Het
Dok2 T C 14: 70,777,516 (GRCm38) F228L probably damaging Het
Eps8 A G 6: 137,514,295 (GRCm38) F362L probably damaging Het
Espn G T 4: 152,138,846 (GRCm38) R250S probably damaging Het
Faf1 A C 4: 109,742,896 (GRCm38) N163H probably damaging Het
Fam198b C A 3: 79,936,674 (GRCm38) S36* probably null Het
Fam71e2 C T 7: 4,757,469 (GRCm38) probably null Het
Fcho1 C T 8: 71,710,481 (GRCm38) V635I probably benign Het
Gm7579 G A 7: 142,211,908 (GRCm38) C17Y unknown Het
Gm996 T C 2: 25,578,753 (GRCm38) Y382C probably damaging Het
Gpx8 G T 13: 113,045,508 (GRCm38) Y130* probably null Het
Gvin1 A T 7: 106,163,436 (GRCm38) Y609N possibly damaging Het
Hectd4 T A 5: 121,305,818 (GRCm38) M30K probably benign Het
Iqub T C 6: 24,450,842 (GRCm38) D586G probably damaging Het
Klhl25 T C 7: 75,867,050 (GRCm38) I568T probably benign Het
Larp6 A G 9: 60,737,810 (GRCm38) E411G probably damaging Het
Lepr A C 4: 101,789,337 (GRCm38) I822L probably benign Het
Lrig3 C A 10: 126,011,052 (GRCm38) D896E probably benign Het
Lrp1 C T 10: 127,553,824 (GRCm38) G3114D probably damaging Het
Macf1 A T 4: 123,486,750 (GRCm38) Y1263N probably damaging Het
Mapk10 T C 5: 102,990,619 (GRCm38) D180G probably damaging Het
Matr3 T A 18: 35,581,640 (GRCm38) V113E probably damaging Het
Mbd4 A T 6: 115,848,926 (GRCm38) F368Y possibly damaging Het
Mcpt8 G A 14: 56,082,280 (GRCm38) R238W probably benign Het
Mcrip2 G T 17: 25,864,647 (GRCm38) T86N possibly damaging Het
Mink1 A G 11: 70,611,592 (GRCm38) N1043S probably damaging Het
Muc4 G A 16: 32,754,616 (GRCm38) G1497R probably benign Het
Muc4 T A 16: 32,754,625 (GRCm38) S1500T probably benign Het
Nbeal2 G A 9: 110,635,194 (GRCm38) T1128I probably benign Het
Nrg2 T A 18: 36,196,547 (GRCm38) Y205F probably damaging Het
Nubp2 A G 17: 24,884,456 (GRCm38) M149T probably benign Het
Olfr1062 T C 2: 86,422,957 (GRCm38) T240A probably damaging Het
Olfr462 T A 11: 87,889,225 (GRCm38) M224L probably damaging Het
Olfr974 G T 9: 39,942,504 (GRCm38) M81I probably benign Het
Pax3 A G 1: 78,192,456 (GRCm38) I191T possibly damaging Het
Pdcd5 T C 7: 35,643,710 (GRCm38) N137D possibly damaging Het
Pik3c2a G A 7: 116,340,156 (GRCm38) A1649V probably damaging Het
Pkhd1l1 T C 15: 44,537,378 (GRCm38) S2183P possibly damaging Het
Plekho1 T A 3: 95,988,993 (GRCm38) Q388L possibly damaging Het
Ppfibp1 A G 6: 146,990,514 (GRCm38) T91A probably benign Het
Ptger4 G T 15: 5,242,606 (GRCm38) N177K probably benign Het
Reln A G 5: 21,901,751 (GRCm38) F3207S probably benign Het
Ripk4 C T 16: 97,751,536 (GRCm38) R194H probably damaging Het
Rnf112 A T 11: 61,452,744 (GRCm38) C112S possibly damaging Het
Rprd1b T G 2: 158,074,935 (GRCm38) Y278* probably null Het
Sel1l A G 12: 91,831,602 (GRCm38) L140P probably damaging Het
Shroom3 G T 5: 92,943,086 (GRCm38) V1151F probably damaging Het
Slc38a3 A G 9: 107,655,064 (GRCm38) V423A probably damaging Het
Slitrk6 T C 14: 110,751,883 (GRCm38) T131A probably damaging Het
Slmap A T 14: 26,460,209 (GRCm38) V323E probably benign Het
Smim6 T C 11: 115,913,504 (GRCm38) V39A probably benign Het
Sorbs1 T C 19: 40,337,005 (GRCm38) T382A probably benign Het
Sparc G A 11: 55,399,211 (GRCm38) T218I possibly damaging Het
Steap1 A T 5: 5,736,589 (GRCm38) F283I probably damaging Het
Stil A G 4: 115,038,474 (GRCm38) T586A probably benign Het
Tbce T A 13: 14,019,795 (GRCm38) D93V probably damaging Het
Tcf12 C T 9: 71,858,840 (GRCm38) G504S probably null Het
Tle4 A T 19: 14,464,345 (GRCm38) I435K probably benign Het
Tmem245 A G 4: 56,899,164 (GRCm38) F254S probably damaging Het
Tmem251 A T 12: 102,744,055 (GRCm38) probably benign Het
Tubgcp3 T C 8: 12,649,722 (GRCm38) K377R probably benign Het
Ush2a A T 1: 188,553,275 (GRCm38) T2003S probably benign Het
Usp53 A G 3: 122,961,363 (GRCm38) S32P possibly damaging Het
Vmn1r78 T A 7: 12,152,756 (GRCm38) L98Q probably damaging Het
Vmn2r116 A C 17: 23,401,803 (GRCm38) Q837P probably benign Het
Vmn2r3 T C 3: 64,275,601 (GRCm38) I226V probably benign Het
Vmn2r84 T A 10: 130,385,843 (GRCm38) D836V probably damaging Het
Vps13d A G 4: 145,087,161 (GRCm38) F165L probably benign Het
Vstm4 A G 14: 32,863,785 (GRCm38) E103G possibly damaging Het
Zfp870 A T 17: 32,883,340 (GRCm38) C339* probably null Het
Other mutations in Dpep3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00158:Dpep3 APN 8 105,979,147 (GRCm38) missense probably benign 0.22
IGL01078:Dpep3 APN 8 105,978,228 (GRCm38) missense probably damaging 1.00
IGL01543:Dpep3 APN 8 105,976,182 (GRCm38) missense probably damaging 1.00
IGL01996:Dpep3 APN 8 105,974,726 (GRCm38) missense probably damaging 1.00
IGL02036:Dpep3 APN 8 105,973,785 (GRCm38) missense probably benign
R0422:Dpep3 UTSW 8 105,976,118 (GRCm38) critical splice donor site probably null
R0627:Dpep3 UTSW 8 105,978,731 (GRCm38) missense possibly damaging 0.56
R0747:Dpep3 UTSW 8 105,977,386 (GRCm38) missense probably benign 0.12
R1116:Dpep3 UTSW 8 105,978,829 (GRCm38) missense probably damaging 0.98
R1711:Dpep3 UTSW 8 105,973,693 (GRCm38) missense probably benign 0.01
R2085:Dpep3 UTSW 8 105,974,714 (GRCm38) missense probably damaging 1.00
R4385:Dpep3 UTSW 8 105,978,186 (GRCm38) missense probably damaging 1.00
R4860:Dpep3 UTSW 8 105,976,189 (GRCm38) missense probably benign 0.34
R5784:Dpep3 UTSW 8 105,978,743 (GRCm38) missense probably benign 0.03
R6658:Dpep3 UTSW 8 105,979,096 (GRCm38) missense probably benign
R6893:Dpep3 UTSW 8 105,973,842 (GRCm38) missense probably benign
R7536:Dpep3 UTSW 8 105,977,400 (GRCm38) missense probably damaging 1.00
R9213:Dpep3 UTSW 8 105,973,613 (GRCm38) missense probably benign 0.15
R9528:Dpep3 UTSW 8 105,977,619 (GRCm38) missense probably benign 0.01
R9766:Dpep3 UTSW 8 105,978,737 (GRCm38) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-03-01