Mutagenetix > Incidental Mutation

Incidental Mutation 'R4860:Muc4'

372457

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

042471-MU

Accession Numbers

Genbank: NM_080457; MGI: 2153525

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4860 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

32735886-32782391 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 32754616 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 1497 (G1497R)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: G1497R

PolyPhen 2 Score 0.354 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: G1497R

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 87.3%

Validation Efficiency

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030K09Rik	T	C	8: 72,455,423 (GRCm38)	S466P	possibly damaging	Het
1700061G19Rik	A	G	17: 56,888,655 (GRCm38)	N684S	probably benign	Het
4930435E12Rik	A	G	16: 38,828,145 (GRCm38)	S201P	probably damaging	Het
Ablim1	T	C	19: 57,079,866 (GRCm38)	T267A	probably damaging	Het
Acap2	A	T	16: 31,103,499 (GRCm38)	L724Q	possibly damaging	Het
Adcy4	T	C	14: 55,781,927 (GRCm38)	T89A	possibly damaging	Het
Agrp	T	C	8: 105,567,368 (GRCm38)	E41G	probably benign	Het
Akr1d1	G	A	6: 37,564,491 (GRCm38)	V308M	probably damaging	Het
Ap1m2	C	T	9: 21,309,674 (GRCm38)	R54Q	probably benign	Het
Arhgap45	T	A	10: 80,027,066 (GRCm38)	V692E	probably damaging	Het
Arid5b	G	T	10: 68,243,095 (GRCm38)	N137K	probably damaging	Het
Arsi	G	A	18: 60,916,651 (GRCm38)	G202E	probably benign	Het
BC048679	T	C	7: 81,495,720 (GRCm38)	N27D	probably benign	Het
Ccdc78	A	G	17: 25,788,700 (GRCm38)	N237S	probably benign	Het
Cd46	G	A	1: 195,062,396 (GRCm38)	L345F	possibly damaging	Het
Cngb3	A	T	4: 19,425,569 (GRCm38)	N459I	possibly damaging	Het
Ctnnd2	A	G	15: 30,881,167 (GRCm38)	E731G	probably damaging	Het
Ctsh	A	G	9: 90,054,548 (GRCm38)	E26G	probably benign	Het
Cul1	G	T	6: 47,517,146 (GRCm38)	K464N	probably benign	Het
Cul1	T	A	6: 47,517,191 (GRCm38)	S479R	probably damaging	Het
Dcaf5	A	T	12: 80,340,232 (GRCm38)	D373E	probably benign	Het
Ddx58	G	T	4: 40,210,000 (GRCm38)	S644R	probably damaging	Het
Dhcr7	A	G	7: 143,840,500 (GRCm38)	Q126R	probably benign	Het
Dok2	T	C	14: 70,777,516 (GRCm38)	F228L	probably damaging	Het
Dpep3	T	G	8: 105,976,189 (GRCm38)	I314L	probably benign	Het
Eps8	A	G	6: 137,514,295 (GRCm38)	F362L	probably damaging	Het
Espn	G	T	4: 152,138,846 (GRCm38)	R250S	probably damaging	Het
Faf1	A	C	4: 109,742,896 (GRCm38)	N163H	probably damaging	Het
Fam198b	C	A	3: 79,936,674 (GRCm38)	S36*	probably null	Het
Fam71e2	C	T	7: 4,757,469 (GRCm38)		probably null	Het
Fcho1	C	T	8: 71,710,481 (GRCm38)	V635I	probably benign	Het
Gm7579	G	A	7: 142,211,908 (GRCm38)	C17Y	unknown	Het
Gm996	T	C	2: 25,578,753 (GRCm38)	Y382C	probably damaging	Het
Gpx8	G	T	13: 113,045,508 (GRCm38)	Y130*	probably null	Het
Gvin1	A	T	7: 106,163,436 (GRCm38)	Y609N	possibly damaging	Het
Hectd4	T	A	5: 121,305,818 (GRCm38)	M30K	probably benign	Het
Iqub	T	C	6: 24,450,842 (GRCm38)	D586G	probably damaging	Het
Klhl25	T	C	7: 75,867,050 (GRCm38)	I568T	probably benign	Het
Larp6	A	G	9: 60,737,810 (GRCm38)	E411G	probably damaging	Het
Lepr	A	C	4: 101,789,337 (GRCm38)	I822L	probably benign	Het
Lrig3	C	A	10: 126,011,052 (GRCm38)	D896E	probably benign	Het
Lrp1	C	T	10: 127,553,824 (GRCm38)	G3114D	probably damaging	Het
Macf1	A	T	4: 123,486,750 (GRCm38)	Y1263N	probably damaging	Het
Mapk10	T	C	5: 102,990,619 (GRCm38)	D180G	probably damaging	Het
Matr3	T	A	18: 35,581,640 (GRCm38)	V113E	probably damaging	Het
Mbd4	A	T	6: 115,848,926 (GRCm38)	F368Y	possibly damaging	Het
Mcpt8	G	A	14: 56,082,280 (GRCm38)	R238W	probably benign	Het
Mcrip2	G	T	17: 25,864,647 (GRCm38)	T86N	possibly damaging	Het
Mink1	A	G	11: 70,611,592 (GRCm38)	N1043S	probably damaging	Het
Nbeal2	G	A	9: 110,635,194 (GRCm38)	T1128I	probably benign	Het
Nrg2	T	A	18: 36,196,547 (GRCm38)	Y205F	probably damaging	Het
Nubp2	A	G	17: 24,884,456 (GRCm38)	M149T	probably benign	Het
Olfr1062	T	C	2: 86,422,957 (GRCm38)	T240A	probably damaging	Het
Olfr462	T	A	11: 87,889,225 (GRCm38)	M224L	probably damaging	Het
Olfr974	G	T	9: 39,942,504 (GRCm38)	M81I	probably benign	Het
Pax3	A	G	1: 78,192,456 (GRCm38)	I191T	possibly damaging	Het
Pdcd5	T	C	7: 35,643,710 (GRCm38)	N137D	possibly damaging	Het
Pik3c2a	G	A	7: 116,340,156 (GRCm38)	A1649V	probably damaging	Het
Pkhd1l1	T	C	15: 44,537,378 (GRCm38)	S2183P	possibly damaging	Het
Plekho1	T	A	3: 95,988,993 (GRCm38)	Q388L	possibly damaging	Het
Ppfibp1	A	G	6: 146,990,514 (GRCm38)	T91A	probably benign	Het
Ptger4	G	T	15: 5,242,606 (GRCm38)	N177K	probably benign	Het
Reln	A	G	5: 21,901,751 (GRCm38)	F3207S	probably benign	Het
Ripk4	C	T	16: 97,751,536 (GRCm38)	R194H	probably damaging	Het
Rnf112	A	T	11: 61,452,744 (GRCm38)	C112S	possibly damaging	Het
Rprd1b	T	G	2: 158,074,935 (GRCm38)	Y278*	probably null	Het
Sel1l	A	G	12: 91,831,602 (GRCm38)	L140P	probably damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Slc38a3	A	G	9: 107,655,064 (GRCm38)	V423A	probably damaging	Het
Slitrk6	T	C	14: 110,751,883 (GRCm38)	T131A	probably damaging	Het
Slmap	A	T	14: 26,460,209 (GRCm38)	V323E	probably benign	Het
Smim6	T	C	11: 115,913,504 (GRCm38)	V39A	probably benign	Het
Sorbs1	T	C	19: 40,337,005 (GRCm38)	T382A	probably benign	Het
Sparc	G	A	11: 55,399,211 (GRCm38)	T218I	possibly damaging	Het
Steap1	A	T	5: 5,736,589 (GRCm38)	F283I	probably damaging	Het
Stil	A	G	4: 115,038,474 (GRCm38)	T586A	probably benign	Het
Tbce	T	A	13: 14,019,795 (GRCm38)	D93V	probably damaging	Het
Tcf12	C	T	9: 71,858,840 (GRCm38)	G504S	probably null	Het
Tle4	A	T	19: 14,464,345 (GRCm38)	I435K	probably benign	Het
Tmem245	A	G	4: 56,899,164 (GRCm38)	F254S	probably damaging	Het
Tmem251	A	T	12: 102,744,055 (GRCm38)		probably benign	Het
Tubgcp3	T	C	8: 12,649,722 (GRCm38)	K377R	probably benign	Het
Ush2a	A	T	1: 188,553,275 (GRCm38)	T2003S	probably benign	Het
Usp53	A	G	3: 122,961,363 (GRCm38)	S32P	possibly damaging	Het
Vmn1r78	T	A	7: 12,152,756 (GRCm38)	L98Q	probably damaging	Het
Vmn2r116	A	C	17: 23,401,803 (GRCm38)	Q837P	probably benign	Het
Vmn2r3	T	C	3: 64,275,601 (GRCm38)	I226V	probably benign	Het
Vmn2r84	T	A	10: 130,385,843 (GRCm38)	D836V	probably damaging	Het
Vps13d	A	G	4: 145,087,161 (GRCm38)	F165L	probably benign	Het
Vstm4	A	G	14: 32,863,785 (GRCm38)	E103G	possibly damaging	Het
Zfp870	A	T	17: 32,883,340 (GRCm38)	C339*	probably null	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,778,812 (GRCm38)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,753,185 (GRCm38)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,750,910 (GRCm38)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,754,347 (GRCm38)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,849 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,848 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,823 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,755,805 (GRCm38)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,753,955 (GRCm38)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,754,071 (GRCm38)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,763,966 (GRCm38)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,750,655 (GRCm38)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,756,627 (GRCm38)	missense	unknown
IGL01625:Muc4	APN	16	32,755,544 (GRCm38)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,736,402 (GRCm38)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,761,348 (GRCm38)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,753,196 (GRCm38)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,751,426 (GRCm38)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,754,265 (GRCm38)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,777,649 (GRCm38)	splice site	probably benign
IGL02210:Muc4	APN	16	32,752,254 (GRCm38)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,750,850 (GRCm38)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,752,076 (GRCm38)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,756,129 (GRCm38)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,775,819 (GRCm38)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,755,282 (GRCm38)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,751,648 (GRCm38)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,750,394 (GRCm38)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,752,048 (GRCm38)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,751,948 (GRCm38)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,751,439 (GRCm38)	nonsense	probably null
IGL03335:Muc4	APN	16	32,753,021 (GRCm38)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,754,318 (GRCm38)	missense	probably benign	0.01
multisplendored	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,770,394 (GRCm38)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,760,251 (GRCm38)	splice site	probably benign
BB004:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
BB014:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
IGL02835:Muc4	UTSW	16	32,763,945 (GRCm38)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,760,248 (GRCm38)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,754,796 (GRCm38)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,756,017 (GRCm38)	missense	unknown
R0119:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,771,604 (GRCm38)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,765,746 (GRCm38)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,752,905 (GRCm38)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,762,536 (GRCm38)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,751,069 (GRCm38)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,756,746 (GRCm38)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,752,107 (GRCm38)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,751,670 (GRCm38)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,769,827 (GRCm38)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,769,847 (GRCm38)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,778,955 (GRCm38)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,756,196 (GRCm38)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,750,525 (GRCm38)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,753,020 (GRCm38)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,752,233 (GRCm38)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,750,349 (GRCm38)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,755,501 (GRCm38)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,756,902 (GRCm38)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,755,933 (GRCm38)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,756,251 (GRCm38)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,756,341 (GRCm38)	missense	unknown
R1928:Muc4	UTSW	16	32,750,532 (GRCm38)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,750,642 (GRCm38)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,751,303 (GRCm38)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,752,254 (GRCm38)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,756,409 (GRCm38)	missense	unknown
R2121:Muc4	UTSW	16	32,760,238 (GRCm38)	missense	unknown
R2139:Muc4	UTSW	16	32,761,225 (GRCm38)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,750,476 (GRCm38)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,766,942 (GRCm38)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,755,891 (GRCm38)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,757,472 (GRCm38)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,755,273 (GRCm38)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,755,826 (GRCm38)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,770,292 (GRCm38)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,756,701 (GRCm38)	missense	unknown
R4523:Muc4	UTSW	16	32,736,336 (GRCm38)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,755,843 (GRCm38)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,757,058 (GRCm38)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,769,277 (GRCm38)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,755,846 (GRCm38)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,751,214 (GRCm38)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,775,903 (GRCm38)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,753,625 (GRCm38)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,751,747 (GRCm38)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,756,098 (GRCm38)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,754,319 (GRCm38)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,041 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,756,296 (GRCm38)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,757,058 (GRCm38)	nonsense	probably null
R5161:Muc4	UTSW	16	32,762,521 (GRCm38)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,751,738 (GRCm38)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,751,666 (GRCm38)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,761,261 (GRCm38)	missense	unknown
R5567:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,750,782 (GRCm38)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,776,807 (GRCm38)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,736,241 (GRCm38)	nonsense	probably null
R5708:Muc4	UTSW	16	32,754,769 (GRCm38)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,751,916 (GRCm38)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,774,839 (GRCm38)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,751,295 (GRCm38)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,751,705 (GRCm38)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,753,052 (GRCm38)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,756,278 (GRCm38)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,759,308 (GRCm38)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,775,783 (GRCm38)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,756,795 (GRCm38)	missense	unknown
R6144:Muc4	UTSW	16	32,766,924 (GRCm38)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,736,140 (GRCm38)	start gained	probably benign
R6268:Muc4	UTSW	16	32,768,767 (GRCm38)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,752,035 (GRCm38)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,753,946 (GRCm38)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,754,358 (GRCm38)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,767,351 (GRCm38)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,736,243 (GRCm38)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,778,946 (GRCm38)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,751,789 (GRCm38)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,759,320 (GRCm38)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,782,008 (GRCm38)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,766,938 (GRCm38)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,775,803 (GRCm38)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,762,518 (GRCm38)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,751,999 (GRCm38)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,751,810 (GRCm38)	nonsense	probably null
R7033:Muc4	UTSW	16	32,756,324 (GRCm38)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,757,091 (GRCm38)	missense
R7123:Muc4	UTSW	16	32,750,691 (GRCm38)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,762,488 (GRCm38)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,756,461 (GRCm38)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,755,336 (GRCm38)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,755,366 (GRCm38)	missense	unknown
R7381:Muc4	UTSW	16	32,780,915 (GRCm38)	missense
R7411:Muc4	UTSW	16	32,751,322 (GRCm38)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,766,950 (GRCm38)	missense
R7539:Muc4	UTSW	16	32,756,396 (GRCm38)	missense
R7544:Muc4	UTSW	16	32,736,198 (GRCm38)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,765,702 (GRCm38)	missense
R7596:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,752,361 (GRCm38)	nonsense	probably null
R7639:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,760,105 (GRCm38)	missense
R7651:Muc4	UTSW	16	32,756,575 (GRCm38)	missense
R7688:Muc4	UTSW	16	32,751,460 (GRCm38)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,753,011 (GRCm38)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,768,734 (GRCm38)	missense
R7763:Muc4	UTSW	16	32,753,311 (GRCm38)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,756,194 (GRCm38)	missense	unknown
R7787:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,751,321 (GRCm38)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
R7930:Muc4	UTSW	16	32,754,078 (GRCm38)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,756,749 (GRCm38)	missense
R8096:Muc4	UTSW	16	32,755,390 (GRCm38)	missense	unknown
R8115:Muc4	UTSW	16	32,755,304 (GRCm38)	missense	unknown
R8162:Muc4	UTSW	16	32,750,379 (GRCm38)	missense	unknown
R8220:Muc4	UTSW	16	32,755,327 (GRCm38)	missense	unknown
R8260:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,754,316 (GRCm38)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,755,897 (GRCm38)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,752,401 (GRCm38)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,753,508 (GRCm38)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,752,993 (GRCm38)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,755,255 (GRCm38)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,781,986 (GRCm38)	missense
R8845:Muc4	UTSW	16	32,756,515 (GRCm38)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,750,643 (GRCm38)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,751,462 (GRCm38)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,753,994 (GRCm38)	missense	probably benign	0.00
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8990:Muc4	UTSW	16	32,752,209 (GRCm38)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,762,536 (GRCm38)	missense
R9034:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,757,468 (GRCm38)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,768,728 (GRCm38)	missense
R9336:Muc4	UTSW	16	32,750,938 (GRCm38)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,751,153 (GRCm38)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,756,618 (GRCm38)	missense
R9429:Muc4	UTSW	16	32,755,724 (GRCm38)	missense	unknown
R9570:Muc4	UTSW	16	32,750,877 (GRCm38)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,756,860 (GRCm38)	missense
R9696:Muc4	UTSW	16	32,753,284 (GRCm38)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,770,270 (GRCm38)	missense
R9743:Muc4	UTSW	16	32,780,824 (GRCm38)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,751,858 (GRCm38)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,751,825 (GRCm38)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,755,481 (GRCm38)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,759,319 (GRCm38)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,756,076 (GRCm38)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,767,393 (GRCm38)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,774,862 (GRCm38)	missense
Z1177:Muc4	UTSW	16	32,767,394 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- GCAGAGGTGACCACATCAAC -3'
(R):5'- CTGAATTTGGTCACGGGAGG -3'

Sequencing Primer
(F):5'- GGTGACCACATCAACTCCTTCAG -3'
(R):5'- CTGAAGGAGTTGATGTGGTTACC -3'

Posted On

2016-03-01