Mutagenetix > Incidental Mutation

Incidental Mutation 'R4829:Zfp882'

372674

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

042445-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.167) question?

Stock #

R4829 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

72662452-72670198 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 72668233 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 353 (H353Q)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably damaging
Transcript: ENSMUST00000110002
AA Change: H353Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: H353Q

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000118290

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170898

Meta Mutation Damage Score

0.8307

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.8%

Validation Efficiency

98% (109/111)

Allele List at MGI

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	T	C	3: 137,774,780 (GRCm39)	V1323A	probably damaging	Het
Abcb1a	T	C	5: 8,773,214 (GRCm39)	L814P	probably damaging	Het
Acat1	C	A	9: 53,502,756 (GRCm39)	G191V	probably damaging	Het
Acsbg3	A	G	17: 57,190,500 (GRCm39)		probably null	Het
Adamts20	A	T	15: 94,224,277 (GRCm39)	D1184E	probably benign	Het
Adss1	T	C	12: 112,601,147 (GRCm39)	L283P	probably damaging	Het
Arid4a	A	G	12: 71,070,272 (GRCm39)		probably null	Het
Arid4b	T	A	13: 14,359,023 (GRCm39)	D599E	probably benign	Het
AW146154	T	C	7: 41,130,057 (GRCm39)	K353R	possibly damaging	Het
Axdnd1	G	T	1: 156,204,216 (GRCm39)	R547S	possibly damaging	Het
Bcat1	A	T	6: 144,961,201 (GRCm39)	F134Y	probably damaging	Het
Camk2d	C	A	3: 126,573,646 (GRCm39)		probably benign	Het
Catsperg2	T	G	7: 29,400,550 (GRCm39)	K268T	probably damaging	Het
Ccar1	A	C	10: 62,581,114 (GRCm39)	F1103L	unknown	Het
Ccdc141	T	A	2: 76,905,260 (GRCm39)	E395D	probably damaging	Het
Cdc37l1	A	G	19: 28,967,983 (GRCm39)	T16A	probably benign	Het
Cdca2	T	A	14: 67,931,202 (GRCm39)		probably null	Het
Cfap96	T	C	8: 46,420,952 (GRCm39)	I159V	probably damaging	Het
Cntn5	T	G	9: 9,976,288 (GRCm39)	K219Q	probably damaging	Het
Col23a1	C	A	11: 51,448,413 (GRCm39)	A202E	unknown	Het
Csmd1	G	A	8: 16,177,310 (GRCm39)	L1318F	probably damaging	Het
Csnk1g3	C	T	18: 54,028,895 (GRCm39)	A16V	possibly damaging	Het
Cspg4b	A	G	13: 113,504,696 (GRCm39)	I1942V	probably benign	Het
Dab2	A	G	15: 6,454,162 (GRCm39)	D224G	probably damaging	Het
Ddr1	A	T	17: 35,996,005 (GRCm39)	C625S	probably damaging	Het
Dennd4a	T	C	9: 64,796,338 (GRCm39)	V788A	probably damaging	Het
Dgcr2	T	C	16: 17,660,617 (GRCm39)	E402G	possibly damaging	Het
Dhcr7	T	G	7: 143,391,654 (GRCm39)	I81S	probably damaging	Het
Exd1	C	T	2: 119,350,807 (GRCm39)	A485T	probably benign	Het
Fat1	C	T	8: 45,489,199 (GRCm39)	T3467I	probably damaging	Het
Fblim1	T	C	4: 141,312,020 (GRCm39)	E235G	probably damaging	Het
Fbxo38	T	A	18: 62,651,662 (GRCm39)	M548L	probably benign	Het
Fstl5	T	C	3: 76,229,489 (GRCm39)	Y97H	probably damaging	Het
Glb1l	A	T	1: 75,176,994 (GRCm39)	S481T	probably damaging	Het
Gp2	A	T	7: 119,056,407 (GRCm39)	V22E	possibly damaging	Het
Grin1	A	G	2: 25,208,736 (GRCm39)	S55P	possibly damaging	Het
Herc2	A	G	7: 55,756,240 (GRCm39)	D760G	probably benign	Het
Hpn	A	G	7: 30,798,300 (GRCm39)		probably benign	Het
Hsf2	T	C	10: 57,372,266 (GRCm39)	V73A	probably damaging	Het
Ighv1-42	T	C	12: 114,900,788 (GRCm39)	Y80C	probably benign	Het
Ighv1-64	T	C	12: 115,471,346 (GRCm39)	K57R	probably benign	Het
Klc1	T	C	12: 111,762,037 (GRCm39)	I569T	probably damaging	Het
Klra3	T	G	6: 130,300,579 (GRCm39)	K263N	probably benign	Het
Lrcol1	C	A	5: 110,502,393 (GRCm39)	H90N	probably benign	Het
Mamdc4	T	C	2: 25,455,368 (GRCm39)	E921G	possibly damaging	Het
Mark1	G	A	1: 184,637,724 (GRCm39)	R622W	possibly damaging	Het
Mdga1	A	G	17: 30,065,343 (GRCm39)	S696P	possibly damaging	Het
Mmp1b	C	T	9: 7,370,729 (GRCm39)		probably null	Het
Mplkipl1	C	T	19: 61,164,364 (GRCm39)	G24R	unknown	Het
Mtnr1a	T	C	8: 45,538,652 (GRCm39)		probably benign	Het
Myo5a	T	C	9: 75,043,689 (GRCm39)	I226T	probably damaging	Het
Ncoa6	G	T	2: 155,257,147 (GRCm39)	P799T	probably damaging	Het
Npc1l1	C	T	11: 6,164,010 (GRCm39)		probably null	Het
Nxph3	T	C	11: 95,402,321 (GRCm39)	E31G	probably benign	Het
Obscn	T	C	11: 58,945,072 (GRCm39)	I4649V	probably null	Het
Or10d4b	A	T	9: 39,534,734 (GRCm39)	H103L	probably damaging	Het
Or8b12b	G	T	9: 37,684,243 (GRCm39)	C96F	probably damaging	Het
Or8g35	A	T	9: 39,381,663 (GRCm39)	Y120N	probably damaging	Het
Or8h10	G	A	2: 86,808,918 (GRCm39)	S74L	probably damaging	Het
P3h3	G	A	6: 124,818,601 (GRCm39)		probably benign	Het
Pcnx2	A	T	8: 126,587,797 (GRCm39)		probably null	Het
Pirb	C	T	7: 3,720,602 (GRCm39)	G299S	probably benign	Het
Pla2g4d	A	G	2: 120,097,224 (GRCm39)	S792P	probably damaging	Het
Plppr4	A	T	3: 117,129,240 (GRCm39)	L76M	possibly damaging	Het
Ppp2r3d	A	T	9: 101,089,709 (GRCm39)	S205T	possibly damaging	Het
Prkdc	G	A	16: 15,519,939 (GRCm39)	D1126N	possibly damaging	Het
Prrt4	G	A	6: 29,177,181 (GRCm39)	S196L	probably benign	Het
Ptpn21	A	C	12: 98,655,555 (GRCm39)	S471A	probably damaging	Het
Ptprk	C	A	10: 28,456,480 (GRCm39)	S9*	probably null	Het
Rassf4	A	T	6: 116,622,103 (GRCm39)	I163K	possibly damaging	Het
Rgs22	C	A	15: 36,104,034 (GRCm39)	R142S	probably damaging	Het
Rit2	C	A	18: 31,345,726 (GRCm39)	L73F	probably damaging	Het
Rrbp1	A	C	2: 143,831,607 (GRCm39)	S187A	probably benign	Het
Rspo2	A	T	15: 42,956,583 (GRCm39)	Y83*	probably null	Het
Scn5a	C	T	9: 119,363,773 (GRCm39)	V456M	probably benign	Het
Scn9a	T	A	2: 66,382,057 (GRCm39)	H290L	probably benign	Het
Smarca4	T	C	9: 21,550,623 (GRCm39)	I452T	probably damaging	Het
Smc2	T	A	4: 52,449,612 (GRCm39)	I198K	probably damaging	Het
Smim14	G	A	5: 65,617,946 (GRCm39)		probably benign	Het
Spg11	A	C	2: 121,938,936 (GRCm39)	N339K	probably benign	Het
Spta1	A	G	1: 174,065,493 (GRCm39)	E2014G	probably benign	Het
Stk4	T	C	2: 163,941,747 (GRCm39)		probably null	Het
Sypl1	C	T	12: 33,017,645 (GRCm39)	T121M	probably damaging	Het
Tas2r120	T	A	6: 132,634,331 (GRCm39)	F138I	probably benign	Het
Tcstv4	G	A	13: 120,769,926 (GRCm39)	C82Y	possibly damaging	Het
Tet2	A	C	3: 133,182,381 (GRCm39)	C1194W	possibly damaging	Het
Tex29	T	C	8: 11,905,668 (GRCm39)		probably benign	Het
Tnfrsf22	T	A	7: 143,197,067 (GRCm39)	T91S	possibly damaging	Het
Tnik	A	T	3: 28,593,690 (GRCm39)		probably benign	Het
Tnr	A	T	1: 159,685,974 (GRCm39)	I402F	probably benign	Het
Unc93b1	A	T	19: 3,994,293 (GRCm39)	S475C	probably damaging	Het
Utrn	T	A	10: 12,539,205 (GRCm39)	E1937D	probably benign	Het
Vmn1r227	A	T	17: 20,955,927 (GRCm39)		noncoding transcript	Het
Vmn2r101	G	A	17: 19,832,229 (GRCm39)	V742I	probably benign	Het
Vmn2r81	T	C	10: 79,083,635 (GRCm39)	L3P	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	72,667,671 (GRCm39)	missense	probably benign
R0244:Zfp882	UTSW	8	72,667,367 (GRCm39)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	72,668,459 (GRCm39)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	72,668,181 (GRCm39)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	72,668,530 (GRCm39)	nonsense	probably null
R1299:Zfp882	UTSW	8	72,667,317 (GRCm39)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	72,667,453 (GRCm39)	missense	probably damaging	0.97
R5028:Zfp882	UTSW	8	72,668,498 (GRCm39)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	72,668,204 (GRCm39)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	72,667,303 (GRCm39)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	72,666,999 (GRCm39)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	72,668,349 (GRCm39)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	72,668,484 (GRCm39)	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	72,668,130 (GRCm39)	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	72,668,517 (GRCm39)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	72,667,093 (GRCm39)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	72,668,073 (GRCm39)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	72,666,985 (GRCm39)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	72,667,962 (GRCm39)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	72,668,831 (GRCm39)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	72,667,915 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTTGAGGAAGGTTGAAAGTCATG -3'
(R):5'- AATCCACTATGTATCCGCTCG -3'

Sequencing Primer
(F):5'- GTTCCCTTCAGTGCCATGAAAAG -3'
(R):5'- CCCCTGTGAATCTGTTCA -3'

Posted On

2016-03-01