|Institutional Source||Beutler Lab|
|Gene Name||CKLF-like MARVEL transmembrane domain containing 8|
|Is this an essential gene?||Probably non essential (E-score: 0.103)|
|Stock #||R4833 (G1)|
|Chromosomal Location||114789345-114844156 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to A at 114796165 bp|
|Amino Acid Change||Arginine to Isoleucine at position 66 (R66I)|
|Ref Sequence||ENSEMBL: ENSMUSP00000043706 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000047013]|
|Predicted Effect||probably benign
AA Change: R66I
PolyPhen 2 Score 0.100 (Sensitivity: 0.93; Specificity: 0.86)
AA Change: R66I
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||99% (74/75)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 3. This gene acts as a tumor suppressor, and plays a role in regulating the migration of tumor cells. The encoded protein is thought to function as a a negative regulator of epidermal growth factor-induced signaling. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cmtm8||
(F):5'- CAAGAAGAGACACCTGCTGT -3'
(R):5'- ATGTCTACATGGGTGGGCA -3'
(F):5'- GACACCTGCTGTCCCAC -3'
(R):5'- GGGCATGTGTGTATAATATGTACAG -3'