|Institutional Source||Beutler Lab|
|Gene Name||host cell factor C2|
|Is this an essential gene?||Probably non essential (E-score: 0.222)|
|Stock #||R4833 (G1)|
|Chromosomal Location||82696160-82742428 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 82709146 bp (GRCm38)|
|Amino Acid Change||Alanine to Valine at position 204 (A204V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000020478 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000020478]|
AA Change: A204V
PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
AA Change: A204V
|Meta Mutation Damage Score||0.9755|
|Coding Region Coverage||
|Validation Efficiency||99% (74/75)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for null or severely hypomorphic allele exhibit reduced poly(I:C)-mediated TLR3 signaling and increased mortality following viral infection. [provided by MGI curators]
|Allele List at MGI||
|Other mutations in this stock||
|Other mutations in Hcfc2||
(F):5'- TTTCTTTTCAAAACCCTGTGTGT -3'
(R):5'- TGCTGACTAAAATCTTAGTGCAATTTG -3'
(F):5'- TGAGATTACAGCTGTGCACC -3'
(R):5'- GATTACCTAAATCAAGCTGCC -3'