Mutagenetix > Incidental Mutation

Incidental Mutation 'R4835:Golgb1'

373189

Institutional Source

Beutler Lab

Gene Symbol

Golgb1

Ensembl Gene

ENSMUSG00000034243

Gene Name

golgi autoantigen, golgin subfamily b, macrogolgin 1

Synonyms

Giantin, C130074L01Rik, F730017E11Rik, Gm6840, 6330407A06Rik

MMRRC Submission

042450-MU

Accession Numbers

Genbank: NM_030035.1

Essential gene?

Probably essential (E-score: 0.911) question?

Stock #

R4835 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

36875140-36933085 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 36891407 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 191 (M191K)

Ref Sequence

ENSEMBL: ENSMUSP00000110460 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039855] [ENSMUST00000114812] [ENSMUST00000134616]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000039855
AA Change: M191K

PolyPhen 2 Score 0.767 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000045239
Gene: ENSMUSG00000034243
AA Change: M191K

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	7.47e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
coiled coil region	130	219	N/A	INTRINSIC
low complexity region	491	512	N/A	INTRINSIC
internal_repeat_3	519	558	7.47e-6	PROSPERO
coiled coil region	563	594	N/A	INTRINSIC
internal_repeat_4	627	661	3.38e-5	PROSPERO
coiled coil region	679	1121	N/A	INTRINSIC
coiled coil region	1153	1240	N/A	INTRINSIC
internal_repeat_4	1253	1288	3.38e-5	PROSPERO
low complexity region	1300	1314	N/A	INTRINSIC
internal_repeat_1	1321	1352	3.51e-6	PROSPERO
low complexity region	1357	1369	N/A	INTRINSIC
coiled coil region	1402	1755	N/A	INTRINSIC
internal_repeat_2	1760	1798	7.47e-6	PROSPERO
internal_repeat_3	1761	1804	7.47e-6	PROSPERO
coiled coil region	1818	2034	N/A	INTRINSIC
low complexity region	2291	2306	N/A	INTRINSIC
internal_repeat_1	2351	2382	3.51e-6	PROSPERO
low complexity region	2400	2418	N/A	INTRINSIC
low complexity region	2538	2549	N/A	INTRINSIC
coiled coil region	2775	2827	N/A	INTRINSIC
coiled coil region	2854	2943	N/A	INTRINSIC
low complexity region	2964	2976	N/A	INTRINSIC
coiled coil region	3007	3057	N/A	INTRINSIC
coiled coil region	3117	3163	N/A	INTRINSIC
transmembrane domain	3215	3237	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114812
AA Change: M191K

PolyPhen 2 Score 0.858 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000110460
Gene: ENSMUSG00000034243
AA Change: M191K

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	6.71e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
low complexity region	120	134	N/A	INTRINSIC
low complexity region	143	154	N/A	INTRINSIC
low complexity region	200	219	N/A	INTRINSIC
low complexity region	450	471	N/A	INTRINSIC
internal_repeat_3	478	517	6.71e-6	PROSPERO
coiled coil region	522	553	N/A	INTRINSIC
internal_repeat_4	586	620	3.05e-5	PROSPERO
coiled coil region	638	1080	N/A	INTRINSIC
coiled coil region	1112	1199	N/A	INTRINSIC
internal_repeat_4	1212	1247	3.05e-5	PROSPERO
low complexity region	1259	1273	N/A	INTRINSIC
internal_repeat_1	1280	1311	3.14e-6	PROSPERO
low complexity region	1316	1328	N/A	INTRINSIC
coiled coil region	1361	1714	N/A	INTRINSIC
internal_repeat_2	1719	1757	6.71e-6	PROSPERO
internal_repeat_3	1720	1763	6.71e-6	PROSPERO
coiled coil region	1777	1993	N/A	INTRINSIC
low complexity region	2250	2265	N/A	INTRINSIC
internal_repeat_1	2310	2341	3.14e-6	PROSPERO
low complexity region	2359	2377	N/A	INTRINSIC
low complexity region	2497	2508	N/A	INTRINSIC
coiled coil region	2734	2786	N/A	INTRINSIC
coiled coil region	2813	2902	N/A	INTRINSIC
low complexity region	2923	2935	N/A	INTRINSIC
coiled coil region	2966	3016	N/A	INTRINSIC
coiled coil region	3076	3122	N/A	INTRINSIC
transmembrane domain	3174	3196	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000134616

SMART Domains

Protein: ENSMUSP00000116503
Gene: ENSMUSG00000034243

Domain	Start	End	E-Value	Type
coiled coil region	42	107	N/A	INTRINSIC
low complexity region	120	134	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135800

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153572

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000231790

Meta Mutation Damage Score

0.1373

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.8%

Validation Efficiency

98% (96/98)

MGI Phenotype

PHENOTYPE: Homozygous knockout affects glycosylation of glycoproteins in the extra-cellular matrix of the palatal shelves, resulting in their failure to elevate and fuse, leading to cleft palate. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, other(2) Gene trapped(5)

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	T	7: 29,574,901 (GRCm38)		noncoding transcript	Het
4933434E20Rik	A	G	3: 90,063,209 (GRCm38)	N17D	probably benign	Het
A1bg	T	C	15: 60,920,251 (GRCm38)	D176G	probably benign	Het
Abcg5	A	T	17: 84,658,648 (GRCm38)	M428K	possibly damaging	Het
Adam1a	A	T	5: 121,519,689 (GRCm38)	C514S	probably damaging	Het
Angptl3	A	G	4: 99,037,412 (GRCm38)	Q325R	probably benign	Het
Ankrd26	C	T	6: 118,548,850 (GRCm38)	W378*	probably null	Het
Apob	T	C	12: 8,015,391 (GRCm38)	V4087A	possibly damaging	Het
Auts2	A	G	5: 131,466,093 (GRCm38)	I117T	probably damaging	Het
AW146154	T	A	7: 41,480,468 (GRCm38)	E408V	probably damaging	Het
Bicd1	A	T	6: 149,484,090 (GRCm38)	N107I	probably benign	Het
Blm	T	C	7: 80,509,546 (GRCm38)	S281G	probably benign	Het
Btaf1	T	A	19: 37,002,458 (GRCm38)	D1542E	probably benign	Het
C2cd2l	A	G	9: 44,315,145 (GRCm38)		probably null	Het
Capn8	C	A	1: 182,604,551 (GRCm38)	N362K	probably damaging	Het
Ccdc14	T	G	16: 34,705,038 (GRCm38)	S186A	probably damaging	Het
Cdca4	A	G	12: 112,821,547 (GRCm38)	L187P	probably damaging	Het
Chil6	T	A	3: 106,405,974 (GRCm38)	K20*	probably null	Het
Cmss1	A	T	16: 57,316,182 (GRCm38)	L109*	probably null	Het
Cmtm6	G	T	9: 114,731,342 (GRCm38)	W36L	probably benign	Het
Cnksr3	T	G	10: 7,160,757 (GRCm38)	I35L	possibly damaging	Het
Col4a2	G	T	8: 11,423,570 (GRCm38)	G534*	probably null	Het
Col5a1	T	G	2: 28,025,644 (GRCm38)	V180G	probably damaging	Het
Ctnna3	T	C	10: 63,581,944 (GRCm38)	F154L	probably benign	Het
Fabp7	G	A	10: 57,785,580 (GRCm38)	V50M	possibly damaging	Het
Fam170a	T	A	18: 50,281,983 (GRCm38)	V232D	probably damaging	Het
Fam186a	T	G	15: 99,945,808 (GRCm38)	T852P	unknown	Het
Fam20a	T	C	11: 109,673,563 (GRCm38)	I455V	probably benign	Het
Fbxl6	C	T	15: 76,536,804 (GRCm38)	V324M	probably damaging	Het
Galnt18	T	C	7: 111,779,523 (GRCm38)	T9A	probably damaging	Het
Gpld1	A	G	13: 24,982,716 (GRCm38)	T650A	probably benign	Het
Gprc5d	A	G	6: 135,116,517 (GRCm38)	F131L	probably benign	Het
H2afv	C	T	11: 6,439,437 (GRCm38)	G7E	unknown	Het
Haus4	A	T	14: 54,545,835 (GRCm38)		probably null	Het
Helq	A	G	5: 100,774,163 (GRCm38)	I725T	possibly damaging	Het
Igsf23	T	A	7: 19,941,830 (GRCm38)	D117V	possibly damaging	Het
Kiz	A	T	2: 146,942,088 (GRCm38)	S514C	probably damaging	Het
Klhl6	A	T	16: 19,957,033 (GRCm38)	N258K	probably benign	Het
Krt72	G	C	15: 101,781,073 (GRCm38)		probably null	Het
Large1	A	T	8: 73,048,347 (GRCm38)	I245N	probably damaging	Het
Lrrc31	T	C	3: 30,679,157 (GRCm38)	I469M	probably damaging	Het
Lypd4	T	A	7: 24,866,696 (GRCm38)	I68F	probably benign	Het
Mast1	A	G	8: 84,923,779 (GRCm38)	S442P	probably damaging	Het
Mmp20	T	A	9: 7,645,299 (GRCm38)	H283Q	probably benign	Het
Mrps5	G	A	2: 127,603,707 (GRCm38)	V385M	possibly damaging	Het
Ms4a15	T	C	19: 10,979,226 (GRCm38)	K241E	possibly damaging	Het
Myt1	A	G	2: 181,797,462 (GRCm38)	E259G	probably damaging	Het
Myt1l	C	A	12: 29,895,305 (GRCm38)	T1023K	unknown	Het
Ncapd3	T	C	9: 27,086,046 (GRCm38)	L1221P	probably damaging	Het
Ncoa6	A	G	2: 155,407,133 (GRCm38)	V1417A	possibly damaging	Het
Nmnat1	A	G	4: 149,473,345 (GRCm38)	V80A	possibly damaging	Het
Nwd2	A	G	5: 63,807,846 (GRCm38)	E1591G	probably benign	Het
Olfr1057	A	G	2: 86,374,509 (GRCm38)	L301S	possibly damaging	Het
Olfr455	A	G	6: 42,538,836 (GRCm38)	F62S	probably damaging	Het
Osbpl1a	A	T	18: 12,768,536 (GRCm38)		probably null	Het
Papln	T	C	12: 83,774,420 (GRCm38)	I204T	probably damaging	Het
Phf1	A	G	17: 26,934,678 (GRCm38)	T97A	probably benign	Het
Plxnb1	A	G	9: 109,105,374 (GRCm38)	D890G	probably damaging	Het
Ppp1r12b	G	T	1: 134,955,733 (GRCm38)	A17E	probably benign	Het
Ptgir	A	G	7: 16,906,869 (GRCm38)	M29V	possibly damaging	Het
Rbp2	G	A	9: 98,507,823 (GRCm38)	C96Y	probably damaging	Het
Rgmb	A	T	17: 15,807,362 (GRCm38)	M365K	possibly damaging	Het
Ric1	A	G	19: 29,595,536 (GRCm38)	N792S	possibly damaging	Het
Ruvbl1	T	C	6: 88,497,229 (GRCm38)	Y405H	possibly damaging	Het
Scaf4	A	G	16: 90,250,307 (GRCm38)	M394T	unknown	Het
Siglech	T	A	7: 55,768,429 (GRCm38)	C48*	probably null	Het
Skiv2l	A	G	17: 34,842,921 (GRCm38)	V695A	possibly damaging	Het
Slc2a5	A	G	4: 150,140,005 (GRCm38)	T320A	probably benign	Het
Slc6a7	T	C	18: 61,002,205 (GRCm38)	Y417C	probably benign	Het
Sox21	A	G	14: 118,234,924 (GRCm38)	C238R	possibly damaging	Het
Tbata	A	T	10: 61,183,353 (GRCm38)	K109N	probably damaging	Het
Tecpr2	T	G	12: 110,954,730 (GRCm38)	S1246A	probably benign	Het
Tekt3	A	G	11: 63,081,259 (GRCm38)	D247G	probably benign	Het
Tenm3	A	T	8: 48,313,236 (GRCm38)		probably null	Het
Thada	A	T	17: 84,441,104 (GRCm38)		probably null	Het
Tpcn2	C	T	7: 145,272,351 (GRCm38)	G204R	probably damaging	Het
Tpm2	T	C	4: 43,519,220 (GRCm38)		probably null	Het
Trbv24	T	C	6: 41,218,092 (GRCm38)		probably benign	Het
Tyw1	A	G	5: 130,277,058 (GRCm38)	R341G	probably benign	Het
Vmn1r183	T	G	7: 24,055,139 (GRCm38)	N122K	probably benign	Het
Vmn2r61	T	A	7: 42,267,035 (GRCm38)	H357Q	possibly damaging	Het
Vps13b	C	T	15: 35,869,372 (GRCm38)	T2674I	probably damaging	Het
Vps13b	T	C	15: 35,910,293 (GRCm38)	L3401P	probably benign	Het
Vps16	T	G	2: 130,438,300 (GRCm38)		probably benign	Het
Vrtn	T	A	12: 84,649,694 (GRCm38)	I406N	probably damaging	Het
Vwa8	A	T	14: 78,934,613 (GRCm38)	Y278F	probably benign	Het
Washc4	A	T	10: 83,579,512 (GRCm38)	I789F	possibly damaging	Het
Zbtb20	C	A	16: 43,618,398 (GRCm38)	T627K	probably damaging	Het
Zfyve16	A	T	13: 92,522,185 (GRCm38)	I406K	probably benign	Het
Zfyve9	A	C	4: 108,717,998 (GRCm38)	S629A	possibly damaging	Het
Zpld1	A	T	16: 55,248,255 (GRCm38)	N164K	probably damaging	Het

Other mutations in Golgb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01394:Golgb1	APN	16	36,931,564 (GRCm38)	missense	probably damaging	1.00
IGL01717:Golgb1	APN	16	36,915,502 (GRCm38)	nonsense	probably null
IGL01965:Golgb1	APN	16	36,917,920 (GRCm38)	missense	probably damaging	1.00
IGL02128:Golgb1	APN	16	36,916,304 (GRCm38)	missense	probably damaging	1.00
IGL02268:Golgb1	APN	16	36,913,128 (GRCm38)	missense	probably benign	0.25
IGL02383:Golgb1	APN	16	36,886,200 (GRCm38)	missense	probably benign	0.01
IGL02444:Golgb1	APN	16	36,907,816 (GRCm38)	splice site	probably benign
IGL02635:Golgb1	APN	16	36,915,013 (GRCm38)	missense	probably benign	0.00
IGL02655:Golgb1	APN	16	36,918,080 (GRCm38)	missense	probably damaging	0.98
IGL02887:Golgb1	APN	16	36,925,849 (GRCm38)	missense	probably damaging	0.99
IGL02937:Golgb1	APN	16	36,916,210 (GRCm38)	missense	probably damaging	1.00
IGL02973:Golgb1	APN	16	36,912,080 (GRCm38)	missense	possibly damaging	0.92
IGL02982:Golgb1	APN	16	36,925,810 (GRCm38)	missense	probably damaging	0.98
IGL03065:Golgb1	APN	16	36,912,866 (GRCm38)	missense	probably benign	0.11
IGL03109:Golgb1	APN	16	36,915,611 (GRCm38)	missense	possibly damaging	0.93
IGL03323:Golgb1	APN	16	36,913,453 (GRCm38)	nonsense	probably null
I2288:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
I2289:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0080:Golgb1	UTSW	16	36,898,611 (GRCm38)	missense	probably damaging	1.00
R0102:Golgb1	UTSW	16	36,875,468 (GRCm38)	intron	probably benign
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0276:Golgb1	UTSW	16	36,913,876 (GRCm38)	missense	probably damaging	1.00
R0394:Golgb1	UTSW	16	36,875,579 (GRCm38)	intron	probably benign
R0469:Golgb1	UTSW	16	36,931,635 (GRCm38)	missense	probably benign	0.41
R0522:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R0575:Golgb1	UTSW	16	36,918,809 (GRCm38)	missense	probably benign
R0600:Golgb1	UTSW	16	36,916,271 (GRCm38)	missense	probably damaging	1.00
R0608:Golgb1	UTSW	16	36,916,330 (GRCm38)	nonsense	probably null
R0711:Golgb1	UTSW	16	36,918,790 (GRCm38)	missense	probably damaging	1.00
R0785:Golgb1	UTSW	16	36,898,790 (GRCm38)	missense	possibly damaging	0.95
R0893:Golgb1	UTSW	16	36,912,277 (GRCm38)	missense	possibly damaging	0.64
R1163:Golgb1	UTSW	16	36,916,126 (GRCm38)	missense	possibly damaging	0.50
R1208:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R1315:Golgb1	UTSW	16	36,914,900 (GRCm38)	missense	probably benign	0.40
R1429:Golgb1	UTSW	16	36,900,563 (GRCm38)	missense	possibly damaging	0.93
R1505:Golgb1	UTSW	16	36,919,643 (GRCm38)	missense	possibly damaging	0.79
R1537:Golgb1	UTSW	16	36,898,788 (GRCm38)	missense	possibly damaging	0.89
R1610:Golgb1	UTSW	16	36,926,101 (GRCm38)	missense	probably benign	0.25
R1659:Golgb1	UTSW	16	36,887,617 (GRCm38)	missense	probably benign	0.01
R1769:Golgb1	UTSW	16	36,916,001 (GRCm38)	missense	probably damaging	1.00
R2105:Golgb1	UTSW	16	36,914,664 (GRCm38)	missense	probably benign
R2212:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R2261:Golgb1	UTSW	16	36,893,360 (GRCm38)	missense	probably damaging	1.00
R2352:Golgb1	UTSW	16	36,898,559 (GRCm38)	missense	probably damaging	0.99
R2357:Golgb1	UTSW	16	36,912,008 (GRCm38)	missense	probably damaging	1.00
R2400:Golgb1	UTSW	16	36,918,466 (GRCm38)	missense	possibly damaging	0.62
R2513:Golgb1	UTSW	16	36,915,151 (GRCm38)	missense	possibly damaging	0.73
R3103:Golgb1	UTSW	16	36,894,849 (GRCm38)	missense	probably damaging	1.00
R3413:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R3748:Golgb1	UTSW	16	36,918,912 (GRCm38)	missense	probably benign	0.00
R3847:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3850:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3936:Golgb1	UTSW	16	36,914,056 (GRCm38)	nonsense	probably null
R3975:Golgb1	UTSW	16	36,918,571 (GRCm38)	missense	probably damaging	0.99
R4025:Golgb1	UTSW	16	36,915,344 (GRCm38)	missense	probably benign	0.00
R4369:Golgb1	UTSW	16	36,916,907 (GRCm38)	missense	probably damaging	1.00
R4518:Golgb1	UTSW	16	36,929,263 (GRCm38)	missense	probably damaging	0.98
R4600:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4610:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4660:Golgb1	UTSW	16	36,887,618 (GRCm38)	missense	probably damaging	0.99
R4811:Golgb1	UTSW	16	36,891,419 (GRCm38)	missense	probably damaging	1.00
R4815:Golgb1	UTSW	16	36,913,115 (GRCm38)	missense	possibly damaging	0.79
R4904:Golgb1	UTSW	16	36,893,386 (GRCm38)	missense	probably damaging	1.00
R4916:Golgb1	UTSW	16	36,916,118 (GRCm38)	missense	probably benign	0.05
R5121:Golgb1	UTSW	16	36,919,258 (GRCm38)	missense	probably damaging	0.99
R5133:Golgb1	UTSW	16	36,891,457 (GRCm38)	missense	possibly damaging	0.75
R5143:Golgb1	UTSW	16	36,898,689 (GRCm38)	missense	probably benign	0.09
R5185:Golgb1	UTSW	16	36,875,141 (GRCm38)	unclassified	probably benign
R5188:Golgb1	UTSW	16	36,918,465 (GRCm38)	missense	probably benign	0.13
R5260:Golgb1	UTSW	16	36,913,141 (GRCm38)	missense	probably benign	0.00
R5297:Golgb1	UTSW	16	36,875,616 (GRCm38)	intron	probably benign
R5386:Golgb1	UTSW	16	36,912,315 (GRCm38)	nonsense	probably null
R5438:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5439:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5494:Golgb1	UTSW	16	36,928,683 (GRCm38)	missense	possibly damaging	0.67
R5592:Golgb1	UTSW	16	36,925,763 (GRCm38)	missense	probably benign	0.02
R5740:Golgb1	UTSW	16	36,919,000 (GRCm38)	missense	probably damaging	0.99
R5862:Golgb1	UTSW	16	36,926,091 (GRCm38)	splice site	silent
R5928:Golgb1	UTSW	16	36,911,987 (GRCm38)	missense	probably damaging	1.00
R6009:Golgb1	UTSW	16	36,914,959 (GRCm38)	missense	probably damaging	1.00
R6062:Golgb1	UTSW	16	36,914,671 (GRCm38)	missense	possibly damaging	0.89
R6102:Golgb1	UTSW	16	36,912,865 (GRCm38)	missense	probably damaging	1.00
R6198:Golgb1	UTSW	16	36,893,395 (GRCm38)	missense	probably damaging	1.00
R6253:Golgb1	UTSW	16	36,915,622 (GRCm38)	missense	possibly damaging	0.77
R6254:Golgb1	UTSW	16	36,913,978 (GRCm38)	missense	probably damaging	0.99
R6321:Golgb1	UTSW	16	36,918,197 (GRCm38)	nonsense	probably null
R6700:Golgb1	UTSW	16	36,875,584 (GRCm38)	intron	probably benign
R6870:Golgb1	UTSW	16	36,918,203 (GRCm38)	missense	probably damaging	1.00
R6882:Golgb1	UTSW	16	36,913,990 (GRCm38)	missense	probably benign
R6944:Golgb1	UTSW	16	36,912,113 (GRCm38)	missense	probably benign
R7108:Golgb1	UTSW	16	36,913,721 (GRCm38)	missense	probably benign	0.01
R7124:Golgb1	UTSW	16	36,913,673 (GRCm38)	missense	probably benign	0.01
R7125:Golgb1	UTSW	16	36,917,963 (GRCm38)	missense	possibly damaging	0.85
R7187:Golgb1	UTSW	16	36,916,150 (GRCm38)	missense	probably benign	0.43
R7205:Golgb1	UTSW	16	36,875,301 (GRCm38)	missense	unknown
R7206:Golgb1	UTSW	16	36,913,749 (GRCm38)	missense	probably benign	0.41
R7233:Golgb1	UTSW	16	36,914,758 (GRCm38)	missense	possibly damaging	0.91
R7320:Golgb1	UTSW	16	36,915,951 (GRCm38)	nonsense	probably null
R7367:Golgb1	UTSW	16	36,898,546 (GRCm38)	missense	probably benign	0.00
R7408:Golgb1	UTSW	16	36,898,547 (GRCm38)	missense	probably damaging	0.98
R7419:Golgb1	UTSW	16	36,912,919 (GRCm38)	missense	possibly damaging	0.95
R7556:Golgb1	UTSW	16	36,915,793 (GRCm38)	missense	probably benign	0.03
R7599:Golgb1	UTSW	16	36,875,396 (GRCm38)	missense	unknown
R7673:Golgb1	UTSW	16	36,913,669 (GRCm38)	missense	probably benign	0.05
R7789:Golgb1	UTSW	16	36,875,399 (GRCm38)	missense	unknown
R7792:Golgb1	UTSW	16	36,918,730 (GRCm38)	missense	probably benign	0.43
R7830:Golgb1	UTSW	16	36,898,721 (GRCm38)	missense	possibly damaging	0.93
R7847:Golgb1	UTSW	16	36,931,920 (GRCm38)	missense	probably damaging	1.00
R7905:Golgb1	UTSW	16	36,913,685 (GRCm38)	missense	probably benign
R7944:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7945:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7950:Golgb1	UTSW	16	36,915,424 (GRCm38)	missense	probably benign	0.13
R8040:Golgb1	UTSW	16	36,913,479 (GRCm38)	missense	possibly damaging	0.85
R8077:Golgb1	UTSW	16	36,918,633 (GRCm38)	missense	probably damaging	0.99
R8181:Golgb1	UTSW	16	36,916,830 (GRCm38)	missense	probably damaging	1.00
R8370:Golgb1	UTSW	16	36,912,317 (GRCm38)	missense	probably benign	0.00
R8684:Golgb1	UTSW	16	36,914,402 (GRCm38)	missense	possibly damaging	0.92
R8725:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8727:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8738:Golgb1	UTSW	16	36,916,313 (GRCm38)	missense	probably damaging	1.00
R8785:Golgb1	UTSW	16	36,919,744 (GRCm38)	missense	probably damaging	0.99
R8824:Golgb1	UTSW	16	36,915,689 (GRCm38)	missense	probably benign
R8825:Golgb1	UTSW	16	36,919,447 (GRCm38)	missense	probably benign	0.00
R8940:Golgb1	UTSW	16	36,916,397 (GRCm38)	missense	probably damaging	1.00
R8962:Golgb1	UTSW	16	36,913,616 (GRCm38)	missense	probably damaging	1.00
R9245:Golgb1	UTSW	16	36,918,819 (GRCm38)	nonsense	probably null
R9365:Golgb1	UTSW	16	36,915,762 (GRCm38)	missense	probably damaging	1.00
R9612:Golgb1	UTSW	16	36,919,605 (GRCm38)	missense	probably benign	0.41
R9620:Golgb1	UTSW	16	36,919,449 (GRCm38)	missense	probably benign
R9691:Golgb1	UTSW	16	36,898,634 (GRCm38)	missense	probably damaging	1.00
R9747:Golgb1	UTSW	16	36,893,407 (GRCm38)	missense	probably damaging	1.00
V1662:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
X0067:Golgb1	UTSW	16	36,914,303 (GRCm38)	nonsense	probably null
Z1088:Golgb1	UTSW	16	36,919,742 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAACTTGTTTATAGAGCATCTCCC -3'
(R):5'- CTCTGAAAGTGACTGCTGGG -3'

Sequencing Primer
(F):5'- GTTGTCAACATTGCCCACG -3'
(R):5'- GGACTGACGTGCCACATTTAAC -3'

Posted On

2016-03-01