Incidental Mutation 'R4439:Slco4a1'
| ID |
374412 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Slco4a1
|
| Ensembl Gene |
ENSMUSG00000038963 |
| Gene Name |
solute carrier organic anion transporter family, member 4a1 |
| Synonyms |
Slc21a12, OATP-E |
| MMRRC Submission |
041704-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.096)
|
| Stock # |
R4439 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
180456245-180474867 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 180472662 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Alanine
at position 549
(V549A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000046502
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000038225]
[ENSMUST00000038259]
|
| AlphaFold |
Q8K078 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000038225
AA Change: V549A
PolyPhen 2
Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)
|
| SMART Domains |
Protein: ENSMUSP00000045023
Gene: ENSMUSG00000038963
AA Change: V549A
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
33 |
46 |
N/A |
INTRINSIC |
|
Pfam:OATP
|
103 |
665 |
5.7e-168 |
PFAM |
|
Pfam:MFS_1
|
105 |
496 |
3e-15 |
PFAM |
|
Pfam:Kazal_2
|
512 |
555 |
1.1e-7 |
PFAM |
|
transmembrane domain
|
668 |
690 |
N/A |
INTRINSIC |
|
low complexity region
|
701 |
718 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000038259
AA Change: V549A
PolyPhen 2
Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)
|
| SMART Domains |
Protein: ENSMUSP00000046502
Gene: ENSMUSG00000038963
AA Change: V549A
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
33 |
46 |
N/A |
INTRINSIC |
|
Pfam:OATP
|
101 |
666 |
2.8e-172 |
PFAM |
|
Pfam:MFS_1
|
105 |
496 |
2.5e-15 |
PFAM |
|
Pfam:Kazal_2
|
512 |
555 |
7.7e-8 |
PFAM |
|
transmembrane domain
|
668 |
690 |
N/A |
INTRINSIC |
|
low complexity region
|
701 |
718 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000128367
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000138446
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000139902
|
| Meta Mutation Damage Score |
0.0874 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.8%
|
| Validation Efficiency |
94% (50/53) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 45 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acacb |
C |
T |
5: 114,246,496 (GRCm38) |
T2237I |
possibly damaging |
Het |
| Adgre1 |
T |
A |
17: 57,447,954 (GRCm38) |
L684Q |
probably damaging |
Het |
| Cfap157 |
T |
A |
2: 32,777,865 (GRCm38) |
Y488F |
probably benign |
Het |
| D630045J12Rik |
A |
G |
6: 38,194,761 (GRCm38) |
I824T |
probably benign |
Het |
| Elmsan1 |
C |
T |
12: 84,156,471 (GRCm38) |
G886S |
probably benign |
Het |
| Eno2 |
T |
C |
6: 124,762,959 (GRCm38) |
|
probably benign |
Het |
| Fam78b |
C |
A |
1: 167,078,922 (GRCm38) |
Q217K |
probably damaging |
Het |
| Fpgs |
C |
T |
2: 32,687,501 (GRCm38) |
C219Y |
probably damaging |
Het |
| Garem2 |
G |
T |
5: 30,113,346 (GRCm38) |
V106L |
possibly damaging |
Het |
| Gm13084 |
A |
T |
4: 143,811,573 (GRCm38) |
V276E |
possibly damaging |
Het |
| Grk3 |
A |
T |
5: 112,946,677 (GRCm38) |
|
probably null |
Het |
| Hint2 |
T |
A |
4: 43,654,919 (GRCm38) |
Y70F |
probably damaging |
Het |
| Hist1h3b |
A |
T |
13: 23,752,725 (GRCm38) |
|
probably null |
Het |
| Ipp |
A |
G |
4: 116,515,077 (GRCm38) |
N101S |
probably benign |
Het |
| Kcnj1 |
A |
T |
9: 32,394,118 (GRCm38) |
|
probably benign |
Het |
| Kcnk4 |
T |
C |
19: 6,932,761 (GRCm38) |
D44G |
probably benign |
Het |
| Kif5c |
T |
C |
2: 49,688,725 (GRCm38) |
S122P |
possibly damaging |
Het |
| Nipbl |
T |
A |
15: 8,338,724 (GRCm38) |
K1171N |
probably damaging |
Het |
| Olfr1285 |
T |
A |
2: 111,409,308 (GRCm38) |
|
noncoding transcript |
Het |
| Pcf11 |
A |
C |
7: 92,658,017 (GRCm38) |
L981R |
probably damaging |
Het |
| Pias2 |
T |
A |
18: 77,097,703 (GRCm38) |
L153H |
probably damaging |
Het |
| Pjvk |
T |
C |
2: 76,651,406 (GRCm38) |
S68P |
probably damaging |
Het |
| Pkd1 |
T |
C |
17: 24,585,692 (GRCm38) |
V3130A |
probably damaging |
Het |
| Plpp4 |
C |
A |
7: 129,257,089 (GRCm38) |
|
probably benign |
Het |
| Plxnd1 |
T |
A |
6: 115,993,976 (GRCm38) |
H277L |
probably damaging |
Het |
| Rasgrf2 |
T |
C |
13: 91,983,678 (GRCm38) |
D620G |
possibly damaging |
Het |
| Rd3l |
C |
A |
12: 111,979,658 (GRCm38) |
S63I |
possibly damaging |
Het |
| Scfd2 |
G |
C |
5: 74,397,707 (GRCm38) |
A503G |
possibly damaging |
Het |
| Tenm4 |
A |
G |
7: 96,895,815 (GRCm38) |
N2375S |
probably benign |
Het |
| Tespa1 |
C |
T |
10: 130,361,957 (GRCm38) |
R283C |
probably damaging |
Het |
| Tle2 |
A |
G |
10: 81,581,682 (GRCm38) |
E227G |
possibly damaging |
Het |
| Tnn |
T |
G |
1: 160,116,080 (GRCm38) |
E1054D |
probably benign |
Het |
| Tnrc6a |
A |
T |
7: 123,152,182 (GRCm38) |
K54* |
probably null |
Het |
| Tns2 |
C |
T |
15: 102,108,934 (GRCm38) |
R281C |
probably damaging |
Het |
| Tpr |
T |
A |
1: 150,403,961 (GRCm38) |
D206E |
probably benign |
Het |
| Ugt2b34 |
A |
T |
5: 86,892,867 (GRCm38) |
F399I |
probably damaging |
Het |
| Usp3 |
A |
T |
9: 66,518,494 (GRCm38) |
D456E |
probably benign |
Het |
| Vmn1r60 |
T |
A |
7: 5,544,489 (GRCm38) |
H204L |
probably damaging |
Het |
| Vmn2r97 |
G |
T |
17: 18,930,354 (GRCm38) |
A488S |
probably benign |
Het |
| Vrk3 |
C |
T |
7: 44,775,442 (GRCm38) |
T427M |
probably benign |
Het |
| Wdr86 |
T |
C |
5: 24,730,237 (GRCm38) |
D36G |
probably damaging |
Het |
| Zc3h4 |
C |
T |
7: 16,429,111 (GRCm38) |
P479S |
unknown |
Het |
| Zfhx4 |
A |
T |
3: 5,214,815 (GRCm38) |
|
probably benign |
Het |
| Zfp607b |
T |
A |
7: 27,702,724 (GRCm38) |
C202S |
probably damaging |
Het |
| Zfp882 |
T |
A |
8: 71,913,609 (GRCm38) |
F93L |
probably damaging |
Het |
|
| Other mutations in Slco4a1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01745:Slco4a1
|
APN |
2 |
180,464,679 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL01982:Slco4a1
|
APN |
2 |
180,473,153 (GRCm38) |
missense |
probably benign |
0.03 |
|
IGL02297:Slco4a1
|
APN |
2 |
180,464,489 (GRCm38) |
missense |
probably benign |
0.16 |
|
IGL02368:Slco4a1
|
APN |
2 |
180,473,128 (GRCm38) |
missense |
probably damaging |
0.98 |
|
conduit
|
UTSW |
2 |
180,473,615 (GRCm38) |
missense |
probably damaging |
1.00 |
|
ingress
|
UTSW |
2 |
180,465,677 (GRCm38) |
missense |
probably benign |
|
|
R1621:Slco4a1
|
UTSW |
2 |
180,471,132 (GRCm38) |
missense |
probably benign |
0.01 |
|
R2275:Slco4a1
|
UTSW |
2 |
180,464,736 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R2472:Slco4a1
|
UTSW |
2 |
180,467,087 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3851:Slco4a1
|
UTSW |
2 |
180,464,091 (GRCm38) |
missense |
probably benign |
0.00 |
|
R3852:Slco4a1
|
UTSW |
2 |
180,464,091 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4271:Slco4a1
|
UTSW |
2 |
180,474,210 (GRCm38) |
missense |
possibly damaging |
0.85 |
|
R4571:Slco4a1
|
UTSW |
2 |
180,464,378 (GRCm38) |
missense |
probably benign |
0.32 |
|
R4732:Slco4a1
|
UTSW |
2 |
180,473,615 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4733:Slco4a1
|
UTSW |
2 |
180,473,615 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4925:Slco4a1
|
UTSW |
2 |
180,472,056 (GRCm38) |
missense |
probably benign |
0.35 |
|
R5156:Slco4a1
|
UTSW |
2 |
180,472,779 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5186:Slco4a1
|
UTSW |
2 |
180,473,108 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5252:Slco4a1
|
UTSW |
2 |
180,464,459 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R5426:Slco4a1
|
UTSW |
2 |
180,471,235 (GRCm38) |
missense |
possibly damaging |
0.84 |
|
R5470:Slco4a1
|
UTSW |
2 |
180,474,114 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5512:Slco4a1
|
UTSW |
2 |
180,474,114 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
R6969:Slco4a1
|
UTSW |
2 |
180,464,808 (GRCm38) |
missense |
probably benign |
|
|
R7133:Slco4a1
|
UTSW |
2 |
180,472,063 (GRCm38) |
missense |
possibly damaging |
0.93 |
|
R7249:Slco4a1
|
UTSW |
2 |
180,464,811 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7357:Slco4a1
|
UTSW |
2 |
180,472,137 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7442:Slco4a1
|
UTSW |
2 |
180,474,126 (GRCm38) |
missense |
probably benign |
|
|
R7599:Slco4a1
|
UTSW |
2 |
180,471,255 (GRCm38) |
missense |
probably benign |
|
|
R7750:Slco4a1
|
UTSW |
2 |
180,471,237 (GRCm38) |
missense |
probably benign |
0.30 |
|
R7834:Slco4a1
|
UTSW |
2 |
180,465,677 (GRCm38) |
missense |
probably benign |
|
|
R8203:Slco4a1
|
UTSW |
2 |
180,464,799 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8504:Slco4a1
|
UTSW |
2 |
180,464,799 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R9166:Slco4a1
|
UTSW |
2 |
180,464,241 (GRCm38) |
missense |
probably benign |
0.00 |
|
R9170:Slco4a1
|
UTSW |
2 |
180,464,685 (GRCm38) |
missense |
probably benign |
0.00 |
|
R9210:Slco4a1
|
UTSW |
2 |
180,472,478 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9455:Slco4a1
|
UTSW |
2 |
180,473,577 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9516:Slco4a1
|
UTSW |
2 |
180,474,150 (GRCm38) |
missense |
possibly damaging |
0.93 |
|
Z1177:Slco4a1
|
UTSW |
2 |
180,464,564 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Slco4a1
|
UTSW |
2 |
180,464,381 (GRCm38) |
missense |
possibly damaging |
0.85 |
|
| Predicted Primers |
PCR Primer
(F):5'- AACGCCATCTACTGTTGCCAG -3'
(R):5'- AGAGCTTCTTACCGTAGAGTAGC -3'
Sequencing Primer
(F):5'- ATCTACTGTTGCCAGCCGAAG -3'
(R):5'- CTTACCGTAGAGTAGCAGTTAGC -3'
|
| Posted On |
2016-03-10 |
Incidental Mutation