Incidental Mutation 'R0281:Lasp1'
ID 37556
Institutional Source Beutler Lab
Gene Symbol Lasp1
Ensembl Gene ENSMUSG00000038366
Gene Name LIM and SH3 protein 1
Synonyms Tg(Col1a1-lacZ)1Ngma, Def-4, Tg(11Col1a1)NG, SH3P6
MMRRC Submission 038503-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.286) question?
Stock # R0281 (G1)
Quality Score 154
Status Validated
Chromosome 11
Chromosomal Location 97799000-97838764 bp(+) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 97806851 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Cysteine to Stop codon at position 32 (C32*)
Ref Sequence ENSEMBL: ENSMUSP00000042123 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000043843] [ENSMUST00000127033] [ENSMUST00000129558] [ENSMUST00000129828] [ENSMUST00000134428] [ENSMUST00000136723] [ENSMUST00000138919] [ENSMUST00000143571] [ENSMUST00000146572] [ENSMUST00000148280] [ENSMUST00000152962] [ENSMUST00000153520]
AlphaFold Q61792
Predicted Effect probably null
Transcript: ENSMUST00000043843
AA Change: C32*
SMART Domains Protein: ENSMUSP00000042123
Gene: ENSMUSG00000038366
AA Change: C32*

LIM 4 56 4.34e-15 SMART
NEBU 62 92 1.1e-8 SMART
NEBU 98 128 1.05e-9 SMART
low complexity region 174 180 N/A INTRINSIC
SH3 207 263 8.11e-17 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000107569
Predicted Effect probably benign
Transcript: ENSMUST00000127033
SMART Domains Protein: ENSMUSP00000127792
Gene: ENSMUSG00000038366

Blast:LIM 1 20 2e-7 BLAST
Pfam:Nebulin 31 59 1.2e-12 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000129558
SMART Domains Protein: ENSMUSP00000123165
Gene: ENSMUSG00000038366

Blast:LIM 1 20 4e-7 BLAST
NEBU 26 56 1.1e-8 SMART
NEBU 62 92 1.05e-9 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000129828
SMART Domains Protein: ENSMUSP00000115308
Gene: ENSMUSG00000038366

Blast:LIM 1 20 4e-7 BLAST
NEBU 26 56 1.1e-8 SMART
NEBU 62 92 1.05e-9 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000134428
Predicted Effect probably benign
Transcript: ENSMUST00000136723
SMART Domains Protein: ENSMUSP00000114306
Gene: ENSMUSG00000038366

Blast:LIM 1 20 2e-7 BLAST
Pfam:Nebulin 31 59 1.2e-12 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000138919
Predicted Effect probably benign
Transcript: ENSMUST00000143571
Predicted Effect probably benign
Transcript: ENSMUST00000146572
SMART Domains Protein: ENSMUSP00000121907
Gene: ENSMUSG00000038366

Blast:LIM 1 20 3e-7 BLAST
Pfam:Nebulin 31 59 2.1e-12 PFAM
Pfam:Nebulin 67 89 1.5e-9 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000148280
SMART Domains Protein: ENSMUSP00000123050
Gene: ENSMUSG00000038366

Blast:LIM 1 20 2e-7 BLAST
NEBU 26 56 1.1e-8 SMART
NEBU 62 92 1.05e-9 SMART
low complexity region 138 144 N/A INTRINSIC
SCOP:d1awj__ 155 186 7e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000152962
SMART Domains Protein: ENSMUSP00000120645
Gene: ENSMUSG00000038366

Blast:LIM 1 20 4e-7 BLAST
NEBU 26 56 1.1e-8 SMART
NEBU 62 92 1.05e-9 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000153520
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.9%
Validation Efficiency 98% (104/106)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced histamine-stimulated gastric acid secretion and enlarged heart and testes on a mixed background. Mice homozygous for a transgene insertion exhibit abnormal tail vertebrae with scoliosis, transient spina bifida occulta, and a bent tail. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 101 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700061G19Rik T A 17: 56,885,169 (GRCm38) Y577* probably null Het
2610507B11Rik C T 11: 78,271,924 (GRCm38) L871F possibly damaging Het
4933430I17Rik A T 4: 62,546,067 (GRCm38) R374* probably null Het
5930422O12Rik A T 8: 33,429,379 (GRCm38) R76* probably null Het
A1cf G A 19: 31,945,814 (GRCm38) A505T probably benign Het
Abcc5 T A 16: 20,422,400 (GRCm38) I12F probably damaging Het
Abcf2 T C 5: 24,566,564 (GRCm38) E555G probably damaging Het
Acan A T 7: 79,100,285 (GRCm38) E1601D probably damaging Het
Adam2 T A 14: 66,037,606 (GRCm38) K559N probably benign Het
Akap11 A C 14: 78,510,089 (GRCm38) D1619E possibly damaging Het
Ankrd11 T C 8: 122,895,568 (GRCm38) D515G probably benign Het
Ankrd27 T A 7: 35,619,371 (GRCm38) N562K probably damaging Het
Atp10b T C 11: 43,153,304 (GRCm38) I119T probably benign Het
Atr T C 9: 95,937,566 (GRCm38) I2202T probably benign Het
BC067074 A T 13: 113,369,143 (GRCm38) I727F probably damaging Het
Brd4 T A 17: 32,213,540 (GRCm38) probably benign Het
Catsperg2 C T 7: 29,706,571 (GRCm38) C634Y possibly damaging Het
Cep192 A G 18: 67,828,482 (GRCm38) probably benign Het
Cfap65 T A 1: 74,927,071 (GRCm38) I366F probably damaging Het
Cnga4 G T 7: 105,407,668 (GRCm38) R326L probably damaging Het
Cntnap5b T A 1: 100,072,153 (GRCm38) M212K probably benign Het
Col6a6 T A 9: 105,784,116 (GRCm38) M265L probably benign Het
Cyp26b1 A T 6: 84,574,556 (GRCm38) F417Y probably damaging Het
Dhx15 A T 5: 52,150,746 (GRCm38) M768K probably benign Het
Drc7 G A 8: 95,071,253 (GRCm38) R433H possibly damaging Het
Duox2 C T 2: 122,292,304 (GRCm38) V550M probably benign Het
Elmo2 A G 2: 165,296,890 (GRCm38) L456P probably damaging Het
Fbxo39 T C 11: 72,317,530 (GRCm38) I236T probably benign Het
Fezf2 A G 14: 12,343,977 (GRCm38) C305R probably damaging Het
Fndc3b C A 3: 27,457,006 (GRCm38) C785F probably benign Het
Gm12253 T C 11: 58,440,012 (GRCm38) probably benign Het
Gnat2 T A 3: 108,095,562 (GRCm38) Y95* probably null Het
Gopc T C 10: 52,350,678 (GRCm38) K220E probably damaging Het
Hectd4 G A 5: 121,254,251 (GRCm38) D193N possibly damaging Het
Hexa G A 9: 59,554,226 (GRCm38) probably null Het
Hspa4l T C 3: 40,785,408 (GRCm38) probably benign Het
Hspa5 T C 2: 34,774,320 (GRCm38) S301P probably damaging Het
Ice1 A T 13: 70,604,047 (GRCm38) S1307T possibly damaging Het
Igtp T C 11: 58,206,054 (GRCm38) L17P probably damaging Het
Itk T C 11: 46,353,916 (GRCm38) Y225C probably damaging Het
Kifc3 A G 8: 95,103,460 (GRCm38) V560A probably damaging Het
Lama1 A G 17: 67,817,569 (GRCm38) N2875D probably damaging Het
Lcp2 T A 11: 34,069,854 (GRCm38) probably benign Het
Lhx9 C T 1: 138,832,904 (GRCm38) G236D probably benign Het
Lrrc38 A T 4: 143,350,409 (GRCm38) I81F probably damaging Het
Ly6a C T 15: 74,995,387 (GRCm38) V94M probably benign Het
Map3k13 A G 16: 21,914,157 (GRCm38) E503G probably damaging Het
Mertk T C 2: 128,782,621 (GRCm38) probably benign Het
Mkl2 T A 16: 13,412,163 (GRCm38) I915N probably damaging Het
Msantd2 G A 9: 37,523,219 (GRCm38) D252N possibly damaging Het
Mtmr12 T A 15: 12,257,706 (GRCm38) L290* probably null Het
Myo3a T C 2: 22,245,598 (GRCm38) I92T probably benign Het
Naglu T A 11: 101,074,027 (GRCm38) N313K probably damaging Het
Nceh1 T C 3: 27,222,804 (GRCm38) V92A possibly damaging Het
Ncf4 A G 15: 78,250,883 (GRCm38) T47A probably damaging Het
Nrp1 T A 8: 128,460,683 (GRCm38) F403L probably damaging Het
Nxph3 T C 11: 95,511,256 (GRCm38) T111A possibly damaging Het
Obscn T A 11: 59,038,615 (GRCm38) E6061V probably damaging Het
Obsl1 C A 1: 75,492,927 (GRCm38) G1149W probably damaging Het
Olfr1162 C T 2: 88,050,412 (GRCm38) V71I possibly damaging Het
Olfr1370 T A 13: 21,072,374 (GRCm38) Y309F probably benign Het
Olfr1487 A G 19: 13,619,485 (GRCm38) T65A probably benign Het
Olfr267 A T 4: 58,784,981 (GRCm38) V247E probably damaging Het
Olfr292 A G 7: 86,694,860 (GRCm38) T135A probably benign Het
Olfr493 A C 7: 108,346,914 (GRCm38) D22E probably benign Het
Olfr814 T A 10: 129,874,546 (GRCm38) L70F possibly damaging Het
Pde9a T C 17: 31,455,106 (GRCm38) V55A probably damaging Het
Pip4k2c A T 10: 127,205,821 (GRCm38) probably null Het
Plvap T C 8: 71,511,382 (GRCm38) N112S probably damaging Het
Pop1 T A 15: 34,529,858 (GRCm38) probably null Het
Ppip5k2 T C 1: 97,716,553 (GRCm38) H1113R possibly damaging Het
Ptprk A T 10: 28,573,392 (GRCm38) I962F probably damaging Het
Rad51ap2 T C 12: 11,457,042 (GRCm38) S322P possibly damaging Het
Rasal1 A G 5: 120,674,605 (GRCm38) T565A probably benign Het
Rbm15 C A 3: 107,331,155 (GRCm38) R642S probably damaging Het
Rpsa G A 9: 120,131,003 (GRCm38) E211K possibly damaging Het
Ryr3 A G 2: 112,686,810 (GRCm38) S3303P probably damaging Het
Scg2 T A 1: 79,435,512 (GRCm38) N458I possibly damaging Het
Setx A G 2: 29,179,643 (GRCm38) T2487A probably benign Het
Slc4a5 G A 6: 83,267,567 (GRCm38) probably benign Het
Slc8a2 T A 7: 16,140,989 (GRCm38) D387E probably benign Het
Smarcc2 A G 10: 128,474,722 (GRCm38) T407A probably benign Het
Snap25 A G 2: 136,777,464 (GRCm38) D179G probably damaging Het
Socs4 T C 14: 47,289,868 (GRCm38) S74P probably benign Het
Sp6 T A 11: 97,021,925 (GRCm38) Y155N probably benign Het
Srrt C T 5: 137,296,127 (GRCm38) probably benign Het
Steap1 C T 5: 5,736,431 (GRCm38) M335I probably benign Het
Stra6 A T 9: 58,145,489 (GRCm38) Y250F probably benign Het
Svil T C 18: 5,094,582 (GRCm38) S1421P probably damaging Het
Tcea3 G A 4: 136,271,366 (GRCm38) C317Y probably damaging Het
Tmco6 T C 18: 36,737,704 (GRCm38) L117S probably damaging Het
Trp53bp1 T C 2: 121,270,237 (GRCm38) K89E probably damaging Het
Trp63 T A 16: 25,764,302 (GRCm38) probably benign Het
Ube2d2a A G 18: 35,800,132 (GRCm38) Y74C probably damaging Het
Usp19 T G 9: 108,498,509 (GRCm38) F885V probably damaging Het
Utp18 T A 11: 93,882,177 (GRCm38) probably benign Het
Vmn2r116 T C 17: 23,401,413 (GRCm38) I707T possibly damaging Het
Vmn2r68 T A 7: 85,233,249 (GRCm38) probably benign Het
Vmn2r68 C G 7: 85,233,258 (GRCm38) probably null Het
Zfp318 C T 17: 46,412,614 (GRCm38) P1848S probably benign Het
Zfp984 G T 4: 147,755,265 (GRCm38) N376K probably benign Het
Other mutations in Lasp1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01296:Lasp1 APN 11 97,836,190 (GRCm38) missense probably damaging 1.00
R2126:Lasp1 UTSW 11 97,836,134 (GRCm38) missense probably benign 0.34
R3906:Lasp1 UTSW 11 97,799,827 (GRCm38) missense probably damaging 1.00
R3908:Lasp1 UTSW 11 97,799,827 (GRCm38) missense probably damaging 1.00
R3909:Lasp1 UTSW 11 97,799,827 (GRCm38) missense probably damaging 1.00
R4908:Lasp1 UTSW 11 97,833,704 (GRCm38) critical splice donor site probably null
R5239:Lasp1 UTSW 11 97,799,860 (GRCm38) missense probably damaging 1.00
R6519:Lasp1 UTSW 11 97,815,557 (GRCm38) splice site probably null
R6576:Lasp1 UTSW 11 97,833,576 (GRCm38) missense probably damaging 1.00
R6629:Lasp1 UTSW 11 97,806,896 (GRCm38) nonsense probably null
R7001:Lasp1 UTSW 11 97,806,833 (GRCm38) missense probably damaging 1.00
R8063:Lasp1 UTSW 11 97,834,131 (GRCm38) missense probably benign 0.00
R8708:Lasp1 UTSW 11 97,806,883 (GRCm38) missense possibly damaging 0.67
R9710:Lasp1 UTSW 11 97,806,767 (GRCm38) start gained probably benign
Predicted Primers PCR Primer

Sequencing Primer
(R):5'- ttaagaaagtcccctcctgtg -3'
Posted On 2013-05-23