Mutagenetix > Incidental Mutation

Incidental Mutation 'R4872:Vmn2r86'

376672

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

042482-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R4872 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130445707-130455894 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 130453591 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 145 (T145K)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably damaging
Transcript: ENSMUST00000170257
AA Change: T145K

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: T145K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.1%

Validation Efficiency

98% (81/83)

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd2	C	A	15: 91,191,311	V100L	probably benign	Het
Acad11	G	A	9: 104,086,266		probably benign	Het
Actb	C	T	5: 142,905,552		probably benign	Het
Ano9	G	A	7: 141,107,204	A374V	probably damaging	Het
Baz2b	T	C	2: 59,942,759		probably null	Het
Bpifb9b	T	A	2: 154,313,631	L350Q	probably damaging	Het
Cd27	T	A	6: 125,234,318		probably null	Het
Cd72	A	G	4: 43,449,563		probably benign	Het
Cdadc1	A	T	14: 59,564,524	S516T	probably benign	Het
Chst5	T	A	8: 111,890,560	I143F	possibly damaging	Het
Col20a1	T	C	2: 180,997,363	V452A	possibly damaging	Het
Cox11	C	A	11: 90,644,403	Q227K	probably benign	Het
Cpa6	A	G	1: 10,595,618		probably null	Het
Dnase1l1	C	T	X: 74,277,038		probably null	Het
Dpm2	T	C	2: 32,571,191		probably benign	Het
Dync1h1	C	A	12: 110,658,126	T3700N	probably damaging	Het
Frem1	A	C	4: 82,963,150	N1273K	probably damaging	Het
Fry	T	C	5: 150,394,239		probably null	Het
Gm10306	G	T	4: 94,556,832		probably benign	Het
Gm5565	T	C	5: 146,158,103	T278A	probably benign	Het
Gm8180	A	G	14: 43,782,345	I40T	probably benign	Het
Iah1	T	C	12: 21,317,425	V44A	probably benign	Het
Iqgap3	C	T	3: 88,113,128	P360S	probably damaging	Het
Klhl28	T	A	12: 64,957,122	I206F	possibly damaging	Het
Krt13	A	G	11: 100,121,506		probably benign	Het
Lipo2	T	A	19: 33,749,514	D41V	probably benign	Het
Lrig2	C	G	3: 104,491,526	V229L	possibly damaging	Het
Lrrc32	C	T	7: 98,498,520	T169I	probably damaging	Het
Lrriq1	T	C	10: 103,178,788	N1053S	possibly damaging	Het
Mfng	C	T	15: 78,764,388	R163H	probably benign	Het
Mgat4c	G	C	10: 102,388,738	R271P	probably damaging	Het
Mylk	A	G	16: 34,914,990	N780S	possibly damaging	Het
N4bp1	T	C	8: 86,861,048	T421A	probably benign	Het
Nat8f1	T	C	6: 85,910,313	T222A	probably benign	Het
Nsun2	T	C	13: 69,543,873		probably benign	Het
Oas2	T	A	5: 120,738,534	D448V	probably damaging	Het
Olfr129	C	A	17: 38,055,576	V6F	probably benign	Het
Olfr168	A	T	16: 19,530,633	C96S	probably damaging	Het
Olfr992	T	C	2: 85,400,428	Y35C	probably damaging	Het
Pgm2l1	T	A	7: 100,227,997	L25Q	probably damaging	Het
Pigp	A	T	16: 94,365,450	V133D	probably benign	Het
Pnkp	C	T	7: 44,862,403	S113L	probably damaging	Het
Pomt2	C	T	12: 87,110,107	D752N	possibly damaging	Het
Ppat	T	C	5: 76,926,793	K65E	probably damaging	Het
Ptprn2	T	C	12: 117,161,694	L616P	probably damaging	Het
Rad54l2	A	G	9: 106,717,892	S289P	probably damaging	Het
Rbm39	G	A	2: 156,177,346	R31C	possibly damaging	Het
Rhbdf2	C	A	11: 116,601,945	V417L	probably benign	Het
Rnf157	T	A	11: 116,355,472	E255D	possibly damaging	Het
Rpl6l	A	T	10: 111,126,443		noncoding transcript	Het
Sec24c	A	G	14: 20,693,745	D1006G	probably damaging	Het
Sh3bp1	G	T	15: 78,908,037	A401S	probably benign	Het
Slc17a8	A	T	10: 89,576,505	D539E	probably benign	Het
Slc38a9	T	A	13: 112,689,564	S136R	probably damaging	Het
Smoc2	A	C	17: 14,369,033	T255P	probably benign	Het
Smyd2	T	C	1: 189,896,650	D152G	probably damaging	Het
Stab1	A	G	14: 31,140,393	V2328A	probably damaging	Het
Taar2	A	G	10: 23,940,693	I44V	probably benign	Het
Tbc1d4	T	C	14: 101,444,708	K1251E	probably benign	Het
Thada	G	A	17: 84,446,599	L315F	probably damaging	Het
Trbv14	T	C	6: 41,135,325	S19P	probably benign	Het
Ttc39c	T	C	18: 12,687,116		probably benign	Het
Ttc39d	A	G	17: 80,217,098	I395M	probably benign	Het
Ttn	T	C	2: 76,718,384	E31891G	probably damaging	Het
Ubr3	T	C	2: 69,970,183	V950A	probably damaging	Het
Uhrf1bp1	G	T	17: 27,890,136	D1110Y	probably benign	Het
Usp9y	T	A	Y: 1,307,920	K2305N	probably damaging	Het
Vmn1r158	G	A	7: 22,790,754	T10I	possibly damaging	Het
Vmn2r78	T	A	7: 86,954,708	I698K	possibly damaging	Het
Vmn2r-ps159	G	T	4: 156,334,397		noncoding transcript	Het
Zfp51	T	C	17: 21,464,671	V516A	probably benign	Het
Zfp654	A	T	16: 64,785,782	S686T	probably benign	Het
Zfp846	T	A	9: 20,590,815	C55S	probably benign	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130453026	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130452496	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130452986	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130446282	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130452989	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130455741	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130448639	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130452912	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130453767	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130446632	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130446854	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130446543	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130452575	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130446396	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130446357	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130446276	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130448574	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130446870	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130453141	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130446386	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130452445	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130446713	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130455725	missense	probably benign
R4049:Vmn2r86	UTSW	10	130447097	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130452976	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130447070	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130455737	missense	probably benign	0.00
R4880:Vmn2r86	UTSW	10	130453615	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130446587	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130446936	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130453666	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130455894	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130446262	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130446926	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130446257	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130448654	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130446531	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130455857	missense	probably benign
R7549:Vmn2r86	UTSW	10	130446828	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130453084	missense	probably benign	0.00
R8257:Vmn2r86	UTSW	10	130452410	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130449986	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130446866	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130453803	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130447065	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130453808	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130446540	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130452571	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130452537	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130446698	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130449833	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TCCTAGAACACAGTATTTGAGCAGTAC -3'
(R):5'- GATGAGATCAACAAGAATCCTGATC -3'

Sequencing Primer
(F):5'- CACAGTATTTGAGCAGTACATCTATG -3'
(R):5'- GAATCCTGATCTTTTACCAAACACC -3'

Posted On

2016-03-17