Incidental Mutation 'R4891:Olfr577'
ID 377310
Institutional Source Beutler Lab
Gene Symbol Olfr577
Ensembl Gene ENSMUSG00000043354
Gene Name olfactory receptor 577
Synonyms MOR7-2, GA_x6K02T2PBJ9-5685322-5684384
MMRRC Submission 042496-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.133) question?
Stock # R4891 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 102971180-102975943 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 102973552 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Leucine at position 147 (I147L)
Ref Sequence ENSEMBL: ENSMUSP00000150712 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051505] [ENSMUST00000185326] [ENSMUST00000214080] [ENSMUST00000215237]
AlphaFold Q8VH11
Predicted Effect probably benign
Transcript: ENSMUST00000051505
AA Change: I147L

PolyPhen 2 Score 0.120 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000059586
Gene: ENSMUSG00000043354
AA Change: I147L

DomainStartEndE-ValueType
Pfam:7tm_4 34 312 3.3e-140 PFAM
Pfam:7TM_GPCR_Srsx 38 310 1.2e-6 PFAM
Pfam:7tm_1 44 295 7.5e-22 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000185326
SMART Domains Protein: ENSMUSP00000142459
Gene: ENSMUSG00000073962

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srsx 35 300 9.7e-12 PFAM
Pfam:7tm_1 41 291 1.8e-29 PFAM
Pfam:7tm_4 140 284 2.6e-28 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000214080
AA Change: I147L

PolyPhen 2 Score 0.120 (Sensitivity: 0.93; Specificity: 0.86)
Predicted Effect probably benign
Transcript: ENSMUST00000215237
AA Change: I147L

PolyPhen 2 Score 0.120 (Sensitivity: 0.93; Specificity: 0.86)
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.6%
  • 20x: 93.3%
Validation Efficiency 100% (39/39)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 34 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts19 A G 18: 59,033,000 Y1088C probably damaging Het
Cercam C A 2: 29,869,271 probably benign Het
Chst1 A G 2: 92,613,992 T270A possibly damaging Het
Cluh C T 11: 74,665,059 T816I possibly damaging Het
Edn3 A G 2: 174,761,732 H91R probably benign Het
Fam135a A G 1: 24,030,328 S487P probably benign Het
Galns T A 8: 122,599,156 D212V possibly damaging Het
Grin2a A G 16: 9,657,706 V582A possibly damaging Het
Hs3st4 T A 7: 124,396,829 N239K possibly damaging Het
Kmt2b C T 7: 30,576,761 W1062* probably null Het
Lrp1 T C 10: 127,541,752 N4110S probably damaging Het
Mag T C 7: 30,900,368 H582R possibly damaging Het
Maml3 C T 3: 51,694,510 probably benign Het
Ndufa5 A G 6: 24,519,247 V26A possibly damaging Het
Nek10 T A 14: 14,860,986 L513M possibly damaging Het
Olfr206 T A 16: 59,345,471 T77S possibly damaging Het
Olfr71 C T 4: 43,706,194 A125T probably damaging Het
Plxdc2 A G 2: 16,712,146 H347R probably benign Het
Ptprn2 A G 12: 117,233,365 probably null Het
Rab3gap2 C T 1: 185,259,366 A683V probably benign Het
Rnf157 A G 11: 116,358,670 V240A probably damaging Het
Slc10a5 A T 3: 10,334,625 V325E possibly damaging Het
Slc5a9 C A 4: 111,891,744 probably null Het
Sptbn2 C T 19: 4,738,469 R1159C probably damaging Het
Stk36 A G 1: 74,603,256 D14G probably damaging Het
Thap2 T C 10: 115,372,696 K173R probably damaging Het
Themis2 G T 4: 132,783,357 Q625K probably benign Het
Vcpip1 G T 1: 9,748,062 P32Q unknown Het
Vmn1r229 A T 17: 20,814,819 T109S probably damaging Het
Vps13b T A 15: 35,640,515 probably null Het
Wdr64 A G 1: 175,698,779 probably benign Het
Wiz A G 17: 32,357,628 S642P possibly damaging Het
Zdhhc1 C T 8: 105,473,017 R383Q probably benign Het
Zfp462 T C 4: 55,060,055 S1194P probably damaging Het
Other mutations in Olfr577
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02246:Olfr577 APN 7 102973744 missense possibly damaging 0.62
IGL03111:Olfr577 APN 7 102973531 missense probably damaging 1.00
R1529:Olfr577 UTSW 7 102973879 missense probably damaging 1.00
R1753:Olfr577 UTSW 7 102973056 missense probably benign
R3005:Olfr577 UTSW 7 102973258 missense possibly damaging 0.56
R4457:Olfr577 UTSW 7 102973527 missense probably damaging 1.00
R4675:Olfr577 UTSW 7 102973806 missense probably damaging 0.99
R4808:Olfr577 UTSW 7 102973911 missense probably damaging 0.99
R4917:Olfr577 UTSW 7 102973407 missense possibly damaging 0.93
R4918:Olfr577 UTSW 7 102973407 missense possibly damaging 0.93
R5328:Olfr577 UTSW 7 102973968 missense possibly damaging 0.46
R6375:Olfr577 UTSW 7 102973753 missense probably damaging 1.00
R6683:Olfr577 UTSW 7 102973713 missense probably benign 0.05
R6958:Olfr577 UTSW 7 102973884 missense possibly damaging 0.67
R7022:Olfr577 UTSW 7 102973968 missense possibly damaging 0.46
R7429:Olfr577 UTSW 7 102973762 missense probably damaging 1.00
R7430:Olfr577 UTSW 7 102973762 missense probably damaging 1.00
R7490:Olfr577 UTSW 7 102973810 missense probably damaging 1.00
R7808:Olfr577 UTSW 7 102973110 missense possibly damaging 0.56
R8169:Olfr577 UTSW 7 102973338 missense probably damaging 0.99
R8544:Olfr577 UTSW 7 102973731 missense probably damaging 1.00
R9027:Olfr577 UTSW 7 102973353 missense probably damaging 1.00
R9265:Olfr577 UTSW 7 102973905 nonsense probably null
X0027:Olfr577 UTSW 7 102973686 missense probably benign 0.05
Z1176:Olfr577 UTSW 7 102973309 missense not run
Z1177:Olfr577 UTSW 7 102973309 missense not run
Predicted Primers PCR Primer
(F):5'- AGAGTCCACTCCCACTGTAG -3'
(R):5'- TCCCTACAGTGCTAGGCATC -3'

Sequencing Primer
(F):5'- TGTAGAAACAATGACGAACATGCC -3'
(R):5'- CCTACAGTGCTAGGCATCTTTTGG -3'
Posted On 2016-03-17