|Institutional Source||Beutler Lab|
|Gene Name||LIM and senescent cell antigen like domains 2|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4893 (G1)|
|Chromosomal Location||31931325-31958619 bp(+) (GRCm38)|
|Type of Mutation||splice site (1186 bp from exon)|
|DNA Base Change (assembly)||T to C at 31941811 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000063670 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000025254] [ENSMUST00000064016] [ENSMUST00000224383] [ENSMUST00000225404]|
|Predicted Effect||possibly damaging
AA Change: I28T
PolyPhen 2 Score 0.803 (Sensitivity: 0.84; Specificity: 0.93)
AA Change: I28T
|Predicted Effect||probably null
|Predicted Effect||probably damaging
AA Change: I23T
PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|Predicted Effect||probably benign
|Predicted Effect||noncoding transcript
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
PHENOTYPE: Homozygous null mice are viable and fertile with no gross abnormalities. Mice homozygous for a different targeted allele exhibit decreased fractional shortening and increased area affected following myocardial infarct. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Lims2||
(F):5'- TAAGTATGTGCCATGTTTGTCACC -3'
(R):5'- AACATCCTGTGGGAGACTCAG -3'
(F):5'- CTTAAAGGTTGGATTCTCCTGGACC -3'
(R):5'- GGGAGACTCAGCTTTGCAG -3'