Mutagenetix > Incidental Mutation

Incidental Mutation 'R4894:Zfp442'

377473

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R4894 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

150407141-150451486 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to T at 150411210 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140098 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

probably null
Transcript: ENSMUST00000109916

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000185796

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf1	C	A	17: 43,299,084 (GRCm38)	Y176*	probably null	Het
Akap13	T	A	7: 75,725,320 (GRCm38)	M1900K	possibly damaging	Het
Ankrd36	A	G	11: 5,635,332 (GRCm38)	E381G	probably damaging	Het
Ap3s1	T	C	18: 46,758,116 (GRCm38)		probably null	Het
Cacna1e	G	T	1: 154,488,805 (GRCm38)	S341*	probably null	Het
Camk1d	G	A	2: 5,354,728 (GRCm38)	S161L	probably damaging	Het
Cdh23	G	T	10: 60,337,851 (GRCm38)	H1619Q	probably benign	Het
Chd7	T	A	4: 8,838,629 (GRCm38)	I1276N	probably damaging	Het
Clca1	A	G	3: 145,013,901 (GRCm38)	V436A	probably damaging	Het
Ctcfl	G	A	2: 173,117,403 (GRCm38)	P177S	probably benign	Het
Dab2ip	A	G	2: 35,730,527 (GRCm38)		probably benign	Het
Dnah8	G	A	17: 30,748,568 (GRCm38)	D2585N	probably benign	Het
Epc1	A	T	18: 6,449,011 (GRCm38)	S495R	probably benign	Het
Espl1	A	G	15: 102,322,323 (GRCm38)		probably null	Het
Eya4	T	C	10: 23,109,854 (GRCm38)	E583G	possibly damaging	Het
Fam111a	C	G	19: 12,588,549 (GRCm38)	T554R	probably benign	Het
Fbxo18	A	T	2: 11,762,960 (GRCm38)	I359N	probably damaging	Het
Fer1l6	T	C	15: 58,618,902 (GRCm38)	C1023R	probably damaging	Het
Gm14139	T	A	2: 150,191,979 (GRCm38)	C104*	probably null	Het
Helz2	G	C	2: 181,236,147 (GRCm38)	P953A	probably benign	Het
Ifi204	G	T	1: 173,760,242 (GRCm38)	S117Y	probably damaging	Het
Igfn1	G	A	1: 135,954,782 (GRCm38)	T2775M	probably damaging	Het
Igsf9	A	G	1: 172,498,067 (GRCm38)	T1101A	probably benign	Het
Ipo13	A	C	4: 117,903,441 (GRCm38)	I614S	probably damaging	Het
Ipo13	A	G	4: 117,904,490 (GRCm38)	I476T	possibly damaging	Het
Kdm2b	C	A	5: 122,940,967 (GRCm38)	E308*	probably null	Het
Klhl20	A	T	1: 161,109,532 (GRCm38)	M91K	possibly damaging	Het
Klrb1f	T	C	6: 129,053,188 (GRCm38)	F64L	probably benign	Het
Ldlrad3	C	T	2: 102,057,948 (GRCm38)	C106Y	probably damaging	Het
Lilra6	T	C	7: 3,912,531 (GRCm38)	T161A	probably benign	Het
Lrriq1	A	T	10: 103,161,752 (GRCm38)	M1334K	possibly damaging	Het
Mepe	C	G	5: 104,325,402 (GRCm38)	P3R	probably damaging	Het
Mgat4e	A	G	1: 134,541,118 (GRCm38)	V396A	probably benign	Het
Nfx1	T	G	4: 40,996,877 (GRCm38)	S651A	probably damaging	Het
Olfr1214	T	C	2: 88,987,439 (GRCm38)	I254M	possibly damaging	Het
Olfr1337	A	T	4: 118,782,286 (GRCm38)	C100S	probably damaging	Het
Olfr1361	T	C	13: 21,659,182 (GRCm38)	N47S	probably damaging	Het
Rag2	T	C	2: 101,629,677 (GRCm38)	S111P	probably damaging	Het
Rai1	T	A	11: 60,186,746 (GRCm38)	D545E	probably damaging	Het
Ralgps1	A	G	2: 33,143,103 (GRCm38)	V498A	possibly damaging	Het
Rasal2	G	A	1: 157,192,804 (GRCm38)	S205L	probably damaging	Het
Rec8	T	C	14: 55,625,330 (GRCm38)	L582P	probably damaging	Het
Retn	G	A	8: 3,657,358 (GRCm38)	R106H	probably damaging	Het
Rnf112	A	G	11: 61,452,662 (GRCm38)	L116P	probably damaging	Het
Rnf213	T	C	11: 119,481,240 (GRCm38)	Y4885H	probably damaging	Het
Sacm1l	A	G	9: 123,582,344 (GRCm38)	I399M	probably benign	Het
Sez6	G	T	11: 77,975,260 (GRCm38)	G738V	probably damaging	Het
Spata17	A	G	1: 187,140,446 (GRCm38)	V56A	probably benign	Het
Spata31d1a	A	T	13: 59,701,728 (GRCm38)	V862D	probably damaging	Het
Sptb	A	G	12: 76,624,994 (GRCm38)		probably null	Het
Srpk2	C	A	5: 23,545,529 (GRCm38)	G59W	probably damaging	Het
Ttc30a1	T	C	2: 75,979,744 (GRCm38)	*665W	probably null	Het
Tyro3	T	C	2: 119,802,298 (GRCm38)	S96P	probably damaging	Het
Ube2v1	A	G	2: 167,610,360 (GRCm38)	S108P	probably damaging	Het
Usp2	C	T	9: 44,075,828 (GRCm38)	S141L	probably benign	Het
Vamp5	T	C	6: 72,370,198 (GRCm38)	D46G	possibly damaging	Het
Vmn1r23	T	A	6: 57,926,325 (GRCm38)	Q156L	probably benign	Het
Vmn2r6	C	T	3: 64,547,408 (GRCm38)	S490N	probably benign	Het
Vps39	A	T	2: 120,352,959 (GRCm38)	I10N	probably damaging	Het
Vwf	C	T	6: 125,645,934 (GRCm38)	Q1755*	probably null	Het
Wdfy4	A	T	14: 33,155,760 (GRCm38)	H82Q	probably benign	Het
Wdr24	T	C	17: 25,826,127 (GRCm38)	Y279H	probably damaging	Het
Wdr72	A	G	9: 74,210,561 (GRCm38)	T852A	probably benign	Het
Zfp1	T	A	8: 111,669,723 (GRCm38)	C92*	probably null	Het
Zfp426	A	T	9: 20,475,073 (GRCm38)		probably benign	Het
Zfp74	T	C	7: 29,936,045 (GRCm38)		probably benign	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150,409,347 (GRCm38)	nonsense	probably null
IGL02566:Zfp442	APN	2	150,409,791 (GRCm38)	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150,409,794 (GRCm38)	splice site	probably benign
LCD18:Zfp442	UTSW	2	150,419,848 (GRCm38)	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150,409,741 (GRCm38)	nonsense	probably null
R0219:Zfp442	UTSW	2	150,411,240 (GRCm38)	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150,411,249 (GRCm38)	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150,408,340 (GRCm38)	nonsense	probably null
R1702:Zfp442	UTSW	2	150,409,180 (GRCm38)	nonsense	probably null
R1829:Zfp442	UTSW	2	150,409,063 (GRCm38)	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150,408,180 (GRCm38)	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150,408,662 (GRCm38)	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150,408,122 (GRCm38)	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150,409,606 (GRCm38)	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150,408,229 (GRCm38)	missense	probably damaging	1.00
R4932:Zfp442	UTSW	2	150,409,715 (GRCm38)	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150,408,495 (GRCm38)	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150,409,610 (GRCm38)	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150,408,159 (GRCm38)	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150,408,024 (GRCm38)	nonsense	probably null
R6042:Zfp442	UTSW	2	150,408,096 (GRCm38)	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150,451,401 (GRCm38)	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150,408,108 (GRCm38)	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150,409,579 (GRCm38)	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150,410,940 (GRCm38)	critical splice donor site	probably null
R7061:Zfp442	UTSW	2	150,408,017 (GRCm38)	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150,408,136 (GRCm38)	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150,409,281 (GRCm38)	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150,409,005 (GRCm38)	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R7591:Zfp442	UTSW	2	150,408,172 (GRCm38)	nonsense	probably null
R7679:Zfp442	UTSW	2	150,410,997 (GRCm38)	nonsense	probably null
R7768:Zfp442	UTSW	2	150,408,321 (GRCm38)	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150,409,719 (GRCm38)	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150,409,482 (GRCm38)	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150,411,226 (GRCm38)	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150,409,176 (GRCm38)	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150,408,709 (GRCm38)	missense	unknown
R8528:Zfp442	UTSW	2	150,409,042 (GRCm38)	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150,408,173 (GRCm38)	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150,409,367 (GRCm38)	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R9401:Zfp442	UTSW	2	150,409,695 (GRCm38)	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150,408,748 (GRCm38)	missense	unknown
R9711:Zfp442	UTSW	2	150,408,287 (GRCm38)	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150,408,479 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGGCATTGGTGTACATGGTT -3'
(R):5'- GTGTTGTCCGTATTACGCTTTC -3'

Sequencing Primer
(F):5'- ATCAGTTTCCACATGCAGATTACC -3'
(R):5'- CGTATTACGCTTTCTCATGTACAC -3'

Posted On

2016-03-17