Mutagenetix > Incidental Mutation

Incidental Mutation 'R4467:Zfp980'

377834

Institutional Source

Beutler Lab

Gene Symbol

Zfp980

Ensembl Gene

ENSMUSG00000058186

Gene Name

zinc finger protein 980

Synonyms

Gm13242

MMRRC Submission

041724-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.265) question?

Stock #

R4467 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

145670685-145704441 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 145702083 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 461 (G461S)

Ref Sequence

ENSEMBL: ENSMUSP00000137589 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105738] [ENSMUST00000180014]

AlphaFold

A2A9J5

Predicted Effect

probably benign
Transcript: ENSMUST00000105738
AA Change: G461S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000101364
Gene: ENSMUSG00000058186
AA Change: G461S

Domain	Start	End	E-Value	Type
KRAB	28	90	1.46e-16	SMART
low complexity region	235	246	N/A	INTRINSIC
ZnF_C2H2	251	273	1.84e-4	SMART
ZnF_C2H2	279	301	3.49e-5	SMART
ZnF_C2H2	307	329	1.95e-3	SMART
ZnF_C2H2	335	357	5.67e-5	SMART
ZnF_C2H2	363	385	1.98e-4	SMART
ZnF_C2H2	391	413	1.98e-4	SMART
ZnF_C2H2	419	441	2.4e-3	SMART
ZnF_C2H2	447	469	1.22e-4	SMART
ZnF_C2H2	475	497	5.67e-5	SMART
ZnF_C2H2	503	525	1.98e-4	SMART
ZnF_C2H2	531	553	6.88e-4	SMART
ZnF_C2H2	559	581	1.58e-3	SMART
ZnF_C2H2	587	609	1.22e-4	SMART
ZnF_C2H2	615	635	1.26e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180014
AA Change: G461S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000137589
Gene: ENSMUSG00000058186
AA Change: G461S

Domain	Start	End	E-Value	Type
KRAB	28	90	1.46e-16	SMART
low complexity region	235	246	N/A	INTRINSIC
ZnF_C2H2	251	273	1.84e-4	SMART
ZnF_C2H2	279	301	3.49e-5	SMART
ZnF_C2H2	307	329	1.95e-3	SMART
ZnF_C2H2	335	357	5.67e-5	SMART
ZnF_C2H2	363	385	1.98e-4	SMART
ZnF_C2H2	391	413	1.98e-4	SMART
ZnF_C2H2	419	441	2.4e-3	SMART
ZnF_C2H2	447	469	1.22e-4	SMART
ZnF_C2H2	475	497	5.67e-5	SMART
ZnF_C2H2	503	525	1.98e-4	SMART
ZnF_C2H2	531	553	6.88e-4	SMART
ZnF_C2H2	559	581	1.58e-3	SMART
ZnF_C2H2	587	609	1.22e-4	SMART
ZnF_C2H2	615	635	1.26e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180968

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 95.0%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	T	5: 63,898,839		probably benign	Het
4930402H24Rik	A	G	2: 130,767,647	I372T	probably damaging	Het
Atg4a-ps	A	G	3: 103,645,855	Y57H	probably damaging	Het
Bag4	C	T	8: 25,769,488	A228T	probably benign	Het
Bms1	G	A	6: 118,383,847	T1220I	probably damaging	Het
Brat1	T	C	5: 140,705,071		probably benign	Het
Casc1	A	T	6: 145,183,218		probably null	Het
Cds2	T	A	2: 132,294,446	Y39*	probably null	Het
Chrnd	T	A	1: 87,197,377	L384Q	probably damaging	Het
Cpa3	A	T	3: 20,228,817	Y155*	probably null	Het
Crlf1	G	A	8: 70,500,956	W260*	probably null	Het
Cux1	C	G	5: 136,312,722	E605D	probably damaging	Het
Cylc2	C	G	4: 51,229,651	T331R	unknown	Het
Dmtf1	T	C	5: 9,136,085	N167S	probably damaging	Het
Dtx2	T	A	5: 136,012,076	W112R	probably damaging	Het
Elf3	A	G	1: 135,256,844	I138T	probably damaging	Het
F11	T	A	8: 45,241,474	I617F	probably damaging	Het
Fdps	A	T	3: 89,100,786	D8E	possibly damaging	Het
Fzd10	C	A	5: 128,601,276	T20K	probably benign	Het
Gm9978	T	A	10: 78,486,916		noncoding transcript	Het
Gpr158	T	A	2: 21,826,999	M970K	probably damaging	Het
Has1	C	T	17: 17,843,995	V461M	probably benign	Het
Hdac3	C	T	18: 37,952,513	G80D	probably benign	Het
Klk12	A	T	7: 43,773,383	R245W	probably damaging	Het
Lamp5	A	G	2: 136,059,020	I47V	probably damaging	Het
Olfr786	T	C	10: 129,437,064	I84T	probably benign	Het
Ovgp1	A	G	3: 105,977,711	D122G	probably benign	Het
Piezo1	T	C	8: 122,486,396	E1875G	probably benign	Het
Pih1d1	A	G	7: 45,158,497	M132V	possibly damaging	Het
Pon2	C	T	6: 5,267,021	A241T	probably benign	Het
Prkce	A	G	17: 86,619,911	I538V	possibly damaging	Het
Rab36	C	T	10: 75,052,043	R249*	probably null	Het
Rps6kl1	C	T	12: 85,147,808	A110T	probably damaging	Het
Rsad1	T	C	11: 94,544,530	T244A	probably benign	Het
Slc22a7	T	C	17: 46,432,510	I532V	probably benign	Het
Slc2a7	T	C	4: 150,163,274	V377A	possibly damaging	Het
Slx4	A	G	16: 3,989,055	V508A	possibly damaging	Het
Stag2	A	G	X: 42,233,872	S400G	probably benign	Het
Stat6	T	G	10: 127,651,228	I201M	probably damaging	Het
Stim2	T	C	5: 54,116,194		probably null	Het
Tbc1d9	A	G	8: 83,210,478	Y63C	probably damaging	Het
Tctn2	T	C	5: 124,620,189		noncoding transcript	Het
Tmem181a	T	A	17: 6,295,786	L185H	probably damaging	Het
Ubr5	T	A	15: 38,004,336	T1282S	probably damaging	Het
Ufl1	A	T	4: 25,254,806	I550N	probably damaging	Het
Uty	A	G	Y: 1,158,372	V557A	possibly damaging	Het
Vmn1r54	T	C	6: 90,269,271	S56P	probably damaging	Het

Other mutations in Zfp980

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00828:Zfp980	APN	4	145701991	missense	probably benign	0.00
R0164:Zfp980	UTSW	4	145701997	missense	probably benign
R0164:Zfp980	UTSW	4	145701997	missense	probably benign
R1186:Zfp980	UTSW	4	145702083	missense	probably benign
R1761:Zfp980	UTSW	4	145702042	missense	probably damaging	1.00
R3726:Zfp980	UTSW	4	145702083	missense	probably benign
R4049:Zfp980	UTSW	4	145702600	missense	probably damaging	1.00
R4610:Zfp980	UTSW	4	145702083	missense	probably benign
R4622:Zfp980	UTSW	4	145702057	missense	probably damaging	1.00
R4873:Zfp980	UTSW	4	145702083	missense	probably benign
R5008:Zfp980	UTSW	4	145702083	missense	probably benign
R5027:Zfp980	UTSW	4	145702083	missense	probably benign
R5497:Zfp980	UTSW	4	145701447	missense	probably damaging	1.00
R5995:Zfp980	UTSW	4	145701909	nonsense	probably null
R6125:Zfp980	UTSW	4	145702638	makesense	probably null
R7353:Zfp980	UTSW	4	145702144	missense	probably benign	0.06
R7675:Zfp980	UTSW	4	145701594	nonsense	probably null
R7939:Zfp980	UTSW	4	145702012	missense	probably damaging	1.00
R8833:Zfp980	UTSW	4	145701026	missense	probably benign	0.00
R9122:Zfp980	UTSW	4	145702264	missense	probably damaging	1.00
R9165:Zfp980	UTSW	4	145701454	missense	possibly damaging	0.95
R9361:Zfp980	UTSW	4	145701429	missense	probably benign	0.08
R9469:Zfp980	UTSW	4	145701117	missense	probably benign	0.14

Predicted Primers

PCR Primer
(F):5'- ACCAATGCAGTGAGTGTGACA -3'
(R):5'- CACTACATTTGTAAGGTTTCTCTCCT -3'

Sequencing Primer
(F):5'- GCTTTACTGACAAAGGCAGTC -3'
(R):5'- GAACTCTCAGATAGCCTTTGTCAG -3'

Posted On

2016-04-11