Incidental Mutation 'R4902:Olfr281'
ID377902
Institutional Source Beutler Lab
Gene Symbol Olfr281
Ensembl Gene ENSMUSG00000032987
Gene Nameolfactory receptor 281
SynonymsMOR160-5, GA_x6K02T2NBG7-5275017-5274082
MMRRC Submission 042505-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.124) question?
Stock #R4902 (G1)
Quality Score225
Status Validated
Chromosome15
Chromosomal Location98452588-98460395 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 98456915 bp
ZygosityHeterozygous
Amino Acid Change Serine to Proline at position 202 (S202P)
Ref Sequence ENSEMBL: ENSMUSP00000150530 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000109163] [ENSMUST00000217517]
Predicted Effect probably damaging
Transcript: ENSMUST00000109163
AA Change: S202P

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000104791
Gene: ENSMUSG00000032987
AA Change: S202P

DomainStartEndE-ValueType
Pfam:7tm_4 31 306 3.8e-54 PFAM
Pfam:7TM_GPCR_Srsx 35 277 2.2e-5 PFAM
Pfam:7tm_1 41 288 2.4e-22 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000217517
AA Change: S202P

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)
Meta Mutation Damage Score 0.3116 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 95.9%
  • 20x: 91.0%
Validation Efficiency 100% (84/84)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930488N24Rik T C 17: 14,106,146 noncoding transcript Het
Adamdec1 T C 14: 68,571,766 N249S probably damaging Het
Asxl1 T A 2: 153,399,831 V767E probably benign Het
Atp6v1f T C 6: 29,470,272 probably benign Het
Brix1 T C 15: 10,483,292 probably null Het
Cap2 T C 13: 46,531,025 V2A probably damaging Het
Chrnb2 A G 3: 89,760,941 C356R probably damaging Het
Chtf8 C T 8: 106,885,792 G172R probably damaging Het
Cybb C G X: 9,450,750 D246H probably benign Het
Dact2 T A 17: 14,196,729 K403M possibly damaging Het
Dhx40 A C 11: 86,771,210 L674V possibly damaging Het
Dlc1 A G 8: 36,577,131 V1230A probably damaging Het
Dnah7b T A 1: 46,290,775 S3260T probably benign Het
Eef1a2 T A 2: 181,148,088 D428V probably benign Het
Ehd1 T C 19: 6,294,243 F178L possibly damaging Het
F2 A T 2: 91,634,971 probably benign Het
Fam186a T C 15: 99,946,842 D507G unknown Het
Fbxo11 G A 17: 88,065,274 probably benign Het
Fry A T 5: 150,495,703 M2871L probably benign Het
Gabrr2 A G 4: 33,095,512 D442G probably damaging Het
Gemin5 A G 11: 58,164,277 I214T probably benign Het
Gfra2 A G 14: 70,967,015 N175S probably damaging Het
Gm8994 T G 6: 136,329,264 V241G probably benign Het
Gprc5c G T 11: 114,864,267 V257L possibly damaging Het
Jhy C T 9: 40,897,525 probably benign Het
Krtap5-5 A T 7: 142,229,419 C165S unknown Het
Lce1k T A 3: 92,806,827 T17S unknown Het
Map3k1 C T 13: 111,772,612 R268Q probably damaging Het
Med13l T A 5: 118,745,130 H1351Q probably damaging Het
Met T C 6: 17,546,996 V876A probably damaging Het
Mical2 T C 7: 112,336,900 S903P probably benign Het
Mkks A G 2: 136,876,174 V396A probably benign Het
Mvk T C 5: 114,455,999 V305A probably benign Het
Myo5a A G 9: 75,174,078 T982A probably benign Het
N4bp1 A G 8: 86,861,683 V209A probably benign Het
Nfkbib G T 7: 28,761,748 S158* probably null Het
Nkx3-1 G A 14: 69,190,918 G72S probably benign Het
Ogfr A G 2: 180,593,725 probably benign Het
Olfr224 G A 11: 58,566,625 S240F possibly damaging Het
Olfr518 G A 7: 108,881,417 T63I probably benign Het
Pcdhga8 T A 18: 37,815,925 D131E probably damaging Het
Plch1 A G 3: 63,740,843 probably benign Het
Polrmt A G 10: 79,746,551 M1T probably null Het
Ppp1r12a T C 10: 108,230,590 V214A probably damaging Het
Prr5l A G 2: 101,797,682 probably benign Het
Prss35 A G 9: 86,756,122 Q315R probably damaging Het
Psg29 G T 7: 17,211,912 *469L probably null Het
Psmd4 A G 3: 95,035,859 V78A probably damaging Het
Rad9a C A 19: 4,201,553 probably benign Het
Rfx7 G T 9: 72,617,291 V588F probably benign Het
Rnase13 A C 14: 51,922,595 I29S probably benign Het
Steap2 T C 5: 5,675,866 N386S possibly damaging Het
Ston1 A G 17: 88,645,252 E719G probably damaging Het
Stx4a A G 7: 127,842,762 probably null Het
Tekt2 A G 4: 126,323,470 S212P possibly damaging Het
Tex19.2 A T 11: 121,116,956 L222Q probably damaging Het
Tmem50a A G 4: 134,909,706 I38T probably damaging Het
Ubr2 A G 17: 46,985,996 V286A possibly damaging Het
Ugt2b35 T C 5: 87,003,300 M255T possibly damaging Het
Vmn2r17 T A 5: 109,453,354 F839L probably benign Het
Wdr36 T C 18: 32,859,261 V617A possibly damaging Het
Wtip A T 7: 34,119,012 probably null Het
Yeats2 G A 16: 20,207,668 G765S probably benign Het
Zbtb3 T C 19: 8,803,967 S315P probably benign Het
Zdhhc8 T C 16: 18,227,166 M259V probably benign Het
Zfp120 A G 2: 150,119,520 probably benign Het
Zfp236 A T 18: 82,609,418 I1552N possibly damaging Het
Other mutations in Olfr281
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00094:Olfr281 APN 15 98456418 missense possibly damaging 0.61
IGL01016:Olfr281 APN 15 98456305 utr 5 prime probably benign
IGL02372:Olfr281 APN 15 98456828 missense probably damaging 1.00
IGL02730:Olfr281 APN 15 98456436 missense probably damaging 1.00
R0732:Olfr281 UTSW 15 98457078 missense possibly damaging 0.94
R1714:Olfr281 UTSW 15 98456733 missense probably damaging 1.00
R1959:Olfr281 UTSW 15 98456753 missense probably damaging 1.00
R4169:Olfr281 UTSW 15 98456997 missense probably benign 0.00
R5014:Olfr281 UTSW 15 98456976 missense possibly damaging 0.80
R6082:Olfr281 UTSW 15 98456766 missense probably damaging 0.97
R6378:Olfr281 UTSW 15 98456544 missense probably benign 0.42
R7383:Olfr281 UTSW 15 98456697 missense probably damaging 1.00
R7850:Olfr281 UTSW 15 98457068 missense probably damaging 1.00
R7853:Olfr281 UTSW 15 98456985 missense probably benign 0.42
R7912:Olfr281 UTSW 15 98456693 missense probably benign 0.00
R7933:Olfr281 UTSW 15 98457068 missense probably damaging 1.00
R7936:Olfr281 UTSW 15 98456985 missense probably benign 0.42
R7993:Olfr281 UTSW 15 98456693 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- AGCAACGAACTCTGTCTGAAG -3'
(R):5'- CGCTGTACTGCAAGGAAGAG -3'

Sequencing Primer
(F):5'- ACGAACTCTGTCTGAAGTTGGTTTTG -3'
(R):5'- TGGGCATAAGATAACGAATCAAGCC -3'
Posted On2016-04-15