Mutagenetix > Incidental Mutation

Incidental Mutation 'R4902:Dact2'

377907

Institutional Source

Beutler Lab

Gene Symbol

Dact2

Ensembl Gene

ENSMUSG00000048826

Gene Name

dishevelled-binding antagonist of beta-catenin 2

Synonyms

dapper2, Dpr2, Frd2, 2900084M21Rik

MMRRC Submission

042505-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4902 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

14195231-14203831 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 14196729 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Methionine at position 403 (K403M)

Ref Sequence

ENSEMBL: ENSMUSP00000051638 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053218]

AlphaFold

Q7TN08

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053218
AA Change: K403M

PolyPhen 2 Score 0.912 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000051638
Gene: ENSMUSG00000048826
AA Change: K403M

Domain	Start	End	E-Value	Type
Pfam:Dapper	19	757	1.8e-231	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.9%
20x: 91.0%

Validation Efficiency

100% (84/84)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele develop normally but display enhanced keratinocyte migration and accelerated re-epithelialization during cutaneous wound healing. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930488N24Rik	T	C	17: 14,106,146		noncoding transcript	Het
Adamdec1	T	C	14: 68,571,766	N249S	probably damaging	Het
Asxl1	T	A	2: 153,399,831	V767E	probably benign	Het
Atp6v1f	T	C	6: 29,470,272		probably benign	Het
Brix1	T	C	15: 10,483,292		probably null	Het
Cap2	T	C	13: 46,531,025	V2A	probably damaging	Het
Chrnb2	A	G	3: 89,760,941	C356R	probably damaging	Het
Chtf8	C	T	8: 106,885,792	G172R	probably damaging	Het
Cybb	C	G	X: 9,450,750	D246H	probably benign	Het
Dhx40	A	C	11: 86,771,210	L674V	possibly damaging	Het
Dlc1	A	G	8: 36,577,131	V1230A	probably damaging	Het
Dnah7b	T	A	1: 46,290,775	S3260T	probably benign	Het
Eef1a2	T	A	2: 181,148,088	D428V	probably benign	Het
Ehd1	T	C	19: 6,294,243	F178L	possibly damaging	Het
F2	A	T	2: 91,634,971		probably benign	Het
Fam186a	T	C	15: 99,946,842	D507G	unknown	Het
Fbxo11	G	A	17: 88,065,274		probably benign	Het
Fry	A	T	5: 150,495,703	M2871L	probably benign	Het
Gabrr2	A	G	4: 33,095,512	D442G	probably damaging	Het
Gemin5	A	G	11: 58,164,277	I214T	probably benign	Het
Gfra2	A	G	14: 70,967,015	N175S	probably damaging	Het
Gm8994	T	G	6: 136,329,264	V241G	probably benign	Het
Gprc5c	G	T	11: 114,864,267	V257L	possibly damaging	Het
Jhy	C	T	9: 40,897,525		probably benign	Het
Krtap5-5	A	T	7: 142,229,419	C165S	unknown	Het
Lce1k	T	A	3: 92,806,827	T17S	unknown	Het
Map3k1	C	T	13: 111,772,612	R268Q	probably damaging	Het
Med13l	T	A	5: 118,745,130	H1351Q	probably damaging	Het
Met	T	C	6: 17,546,996	V876A	probably damaging	Het
Mical2	T	C	7: 112,336,900	S903P	probably benign	Het
Mkks	A	G	2: 136,876,174	V396A	probably benign	Het
Mvk	T	C	5: 114,455,999	V305A	probably benign	Het
Myo5a	A	G	9: 75,174,078	T982A	probably benign	Het
N4bp1	A	G	8: 86,861,683	V209A	probably benign	Het
Nfkbib	G	T	7: 28,761,748	S158*	probably null	Het
Nkx3-1	G	A	14: 69,190,918	G72S	probably benign	Het
Ogfr	A	G	2: 180,593,725		probably benign	Het
Olfr224	G	A	11: 58,566,625	S240F	possibly damaging	Het
Olfr281	T	C	15: 98,456,915	S202P	probably damaging	Het
Olfr518	G	A	7: 108,881,417	T63I	probably benign	Het
Pcdhga8	T	A	18: 37,815,925	D131E	probably damaging	Het
Plch1	A	G	3: 63,740,843		probably benign	Het
Polrmt	A	G	10: 79,746,551	M1T	probably null	Het
Ppp1r12a	T	C	10: 108,230,590	V214A	probably damaging	Het
Prr5l	A	G	2: 101,797,682		probably benign	Het
Prss35	A	G	9: 86,756,122	Q315R	probably damaging	Het
Psg29	G	T	7: 17,211,912	*469L	probably null	Het
Psmd4	A	G	3: 95,035,859	V78A	probably damaging	Het
Rad9a	C	A	19: 4,201,553		probably benign	Het
Rfx7	G	T	9: 72,617,291	V588F	probably benign	Het
Rnase13	A	C	14: 51,922,595	I29S	probably benign	Het
Steap2	T	C	5: 5,675,866	N386S	possibly damaging	Het
Ston1	A	G	17: 88,645,252	E719G	probably damaging	Het
Stx4a	A	G	7: 127,842,762		probably null	Het
Tekt2	A	G	4: 126,323,470	S212P	possibly damaging	Het
Tex19.2	A	T	11: 121,116,956	L222Q	probably damaging	Het
Tmem50a	A	G	4: 134,909,706	I38T	probably damaging	Het
Ubr2	A	G	17: 46,985,996	V286A	possibly damaging	Het
Ugt2b35	T	C	5: 87,003,300	M255T	possibly damaging	Het
Vmn2r17	T	A	5: 109,453,354	F839L	probably benign	Het
Wdr36	T	C	18: 32,859,261	V617A	possibly damaging	Het
Wtip	A	T	7: 34,119,012		probably null	Het
Yeats2	G	A	16: 20,207,668	G765S	probably benign	Het
Zbtb3	T	C	19: 8,803,967	S315P	probably benign	Het
Zdhhc8	T	C	16: 18,227,166	M259V	probably benign	Het
Zfp120	A	G	2: 150,119,520		probably benign	Het
Zfp236	A	T	18: 82,609,418	I1552N	possibly damaging	Het

Other mutations in Dact2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01867:Dact2	APN	17	14195670	missense	probably damaging	0.98
R0597:Dact2	UTSW	17	14197041	missense	probably benign
R1657:Dact2	UTSW	17	14197990	missense	probably benign	0.03
R1717:Dact2	UTSW	17	14197913	missense	probably benign	0.11
R1734:Dact2	UTSW	17	14196639	missense	probably benign	0.00
R1883:Dact2	UTSW	17	14197823	missense	possibly damaging	0.81
R2238:Dact2	UTSW	17	14197050	missense	probably damaging	0.99
R2878:Dact2	UTSW	17	14195914	missense	probably damaging	1.00
R4290:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4291:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R5029:Dact2	UTSW	17	14195852	missense	probably benign	0.00
R5084:Dact2	UTSW	17	14197952	missense	possibly damaging	0.94
R5436:Dact2	UTSW	17	14195748	missense	probably damaging	1.00
R5837:Dact2	UTSW	17	14196253	missense	probably damaging	1.00
R5847:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R6048:Dact2	UTSW	17	14197305	missense	probably damaging	1.00
R6377:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R7013:Dact2	UTSW	17	14203534	missense	probably benign
R7268:Dact2	UTSW	17	14196535	missense	probably benign	0.01
R7425:Dact2	UTSW	17	14196331	missense	probably damaging	1.00
R8725:Dact2	UTSW	17	14196884	nonsense	probably null
R8727:Dact2	UTSW	17	14196884	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GGAAAAGCCCATCCTCAGCTTC -3'
(R):5'- AGCTGCTTTTCCACCGAAGC -3'

Sequencing Primer
(F):5'- TTCACGGGAGAGGTTTCACCAG -3'
(R):5'- TTCCACCGAAGCCCTGTG -3'

Posted On

2016-04-15