Mutagenetix > Incidental Mutation

Incidental Mutation 'R4917:Arhgef40'

378330

Institutional Source

Beutler Lab

Gene Symbol

Arhgef40

Ensembl Gene

ENSMUSG00000004562

Gene Name

Rho guanine nucleotide exchange factor 40

Synonyms

E130112L23Rik

MMRRC Submission

042519-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R4917 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52222176-52243708 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 52227556 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 434 (E434G)

Ref Sequence

ENSEMBL: ENSMUSP00000138354 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093813] [ENSMUST00000100639] [ENSMUST00000182061] [ENSMUST00000182193] [ENSMUST00000182338] [ENSMUST00000183208] [ENSMUST00000182905] [ENSMUST00000182909] [ENSMUST00000182760] [ENSMUST00000182649]

AlphaFold

Q3UPH7

Predicted Effect

probably damaging
Transcript: ENSMUST00000093813
AA Change: E497G

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000091331
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	773	792	N/A	INTRINSIC
low complexity region	885	914	N/A	INTRINSIC
low complexity region	958	996	N/A	INTRINSIC
Pfam:RhoGEF	1087	1246	6.1e-9	PFAM
PH	1264	1372	3.97e-8	SMART
low complexity region	1403	1424	N/A	INTRINSIC
low complexity region	1478	1491	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100639
AA Change: E497G

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000098204
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	773	792	N/A	INTRINSIC
low complexity region	885	914	N/A	INTRINSIC
low complexity region	958	996	N/A	INTRINSIC
Pfam:RhoGEF	1087	1246	5.9e-9	PFAM
PH	1264	1372	3.97e-8	SMART
low complexity region	1430	1443	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181008

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182019

Predicted Effect

probably damaging
Transcript: ENSMUST00000182061
AA Change: E497G

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000138128
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	773	792	N/A	INTRINSIC
low complexity region	885	914	N/A	INTRINSIC
low complexity region	958	996	N/A	INTRINSIC
Pfam:RhoGEF	1087	1247	3.7e-9	PFAM
PH	1264	1372	3.97e-8	SMART
low complexity region	1430	1443	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182193

Predicted Effect

probably benign
Transcript: ENSMUST00000182338

SMART Domains

Protein: ENSMUSP00000138482
Gene: ENSMUSG00000004562

Domain	Start	End	E-Value	Type
low complexity region	72	85	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182961

Predicted Effect

probably damaging
Transcript: ENSMUST00000183208
AA Change: E434G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000138354
Gene: ENSMUSG00000004562
AA Change: E434G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000182905
AA Change: E497G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000138797
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	773	792	N/A	INTRINSIC
low complexity region	885	914	N/A	INTRINSIC
low complexity region	958	996	N/A	INTRINSIC
SCOP:d1kz7a1	1073	1162	4e-7	SMART
Blast:RhoGEF	1087	1157	1e-8	BLAST

Predicted Effect

probably damaging
Transcript: ENSMUST00000182909
AA Change: E497G

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000138635
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	773	792	N/A	INTRINSIC
low complexity region	885	914	N/A	INTRINSIC
low complexity region	958	996	N/A	INTRINSIC
Pfam:RhoGEF	1087	1247	3.9e-9	PFAM
PH	1264	1372	3.97e-8	SMART
low complexity region	1403	1424	N/A	INTRINSIC
low complexity region	1478	1491	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000182760
AA Change: E497G

PolyPhen 2 Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000138125
Gene: ENSMUSG00000004562
AA Change: E497G

Domain	Start	End	E-Value	Type
low complexity region	201	223	N/A	INTRINSIC
low complexity region	306	320	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
low complexity region	351	395	N/A	INTRINSIC
low complexity region	451	472	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	581	606	N/A	INTRINSIC
low complexity region	782	801	N/A	INTRINSIC
low complexity region	894	923	N/A	INTRINSIC
low complexity region	967	1005	N/A	INTRINSIC
Pfam:RhoGEF	1096	1256	5.9e-9	PFAM
PH	1273	1381	3.97e-8	SMART
low complexity region	1412	1433	N/A	INTRINSIC
low complexity region	1487	1500	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182480

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182740

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182609

Predicted Effect

probably benign
Transcript: ENSMUST00000182412

Predicted Effect

probably benign
Transcript: ENSMUST00000182649

Meta Mutation Damage Score

0.1114

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.1%
20x: 91.7%

Validation Efficiency

97% (111/114)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein similar to guanosine nucleotide exchange factors for Rho GTPases. The encoded protein contains in its C-terminus a GEF domain involved in exchange activity and a pleckstrin homology domain. Alternatively spliced transcripts that encode different proteins have been described. [provided by RefSeq, Mar 2014]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	C	T	13: 81,658,996 (GRCm39)	V2783I	probably benign	Het
Ago4	A	C	4: 126,400,635 (GRCm39)	C693G	probably damaging	Het
Apobec2	T	C	17: 48,730,153 (GRCm39)	E171G	probably benign	Het
Arfgef3	T	A	10: 18,492,638 (GRCm39)	I1258F	probably damaging	Het
Bcas1	T	C	2: 170,220,806 (GRCm39)	D324G	probably damaging	Het
Bdp1	A	T	13: 100,191,713 (GRCm39)	V1422E	probably damaging	Het
Bfsp1	C	T	2: 143,669,391 (GRCm39)	R396Q	probably benign	Het
Bpifb9b	T	A	2: 154,156,026 (GRCm39)		probably null	Het
Cacna1s	T	A	1: 136,029,302 (GRCm39)		probably null	Het
Casp8	T	C	1: 58,866,377 (GRCm39)	F126S	probably damaging	Het
Cdh26	T	C	2: 178,091,614 (GRCm39)	S58P	probably benign	Het
Cds2	A	G	2: 132,140,398 (GRCm39)	T182A	probably damaging	Het
Cfap157	C	T	2: 32,669,965 (GRCm39)	R206H	probably benign	Het
Col7a1	G	A	9: 108,795,532 (GRCm39)	G1529E	unknown	Het
Crybg3	A	G	16: 59,350,782 (GRCm39)	F2567L	probably benign	Het
Cyp2e1	T	C	7: 140,354,527 (GRCm39)	S393P	possibly damaging	Het
D5Ertd579e	A	G	5: 36,773,160 (GRCm39)	Y412H	probably damaging	Het
Dclk2	A	T	3: 86,732,049 (GRCm39)		probably null	Het
Ddx50	A	T	10: 62,463,450 (GRCm39)	C414*	probably null	Het
Dip2c	T	A	13: 9,671,905 (GRCm39)		probably null	Het
Dmtn	G	T	14: 70,843,159 (GRCm39)	P283Q	probably damaging	Het
Dspp	C	A	5: 104,325,789 (GRCm39)	D717E	unknown	Het
Efcab11	T	C	12: 99,685,321 (GRCm39)	D151G	probably damaging	Het
Egfem1	T	C	3: 29,206,042 (GRCm39)	V93A	probably damaging	Het
Ehbp1	T	C	11: 22,096,592 (GRCm39)	D299G	probably benign	Het
Erlec1	A	G	11: 30,884,710 (GRCm39)	Y448H	possibly damaging	Het
Fer1l4	T	C	2: 155,873,220 (GRCm39)	K1287E	probably benign	Het
Fn1	A	T	1: 71,634,968 (GRCm39)		probably null	Het
Fut8	G	T	12: 77,521,818 (GRCm39)	A486S	probably damaging	Het
Gfra1	A	T	19: 58,255,522 (GRCm39)	S308R	probably damaging	Het
Gm10764	A	G	10: 87,126,579 (GRCm39)		noncoding transcript	Het
Gm5958	C	A	14: 100,073,189 (GRCm39)		noncoding transcript	Het
Gm6788	C	T	19: 28,740,664 (GRCm39)		noncoding transcript	Het
Gsdmd	A	G	15: 75,736,241 (GRCm39)	I123M	probably benign	Het
H2bc13	A	T	13: 21,900,189 (GRCm39)	V42E	probably damaging	Het
Hsf4	A	G	8: 105,999,367 (GRCm39)	E235G	probably benign	Het
Ighv1-83	T	C	12: 115,927,580 (GRCm39)	I57V	probably benign	Het
Inhca	A	C	9: 103,129,054 (GRCm39)	Y235*	probably null	Het
Inpp5f	A	T	7: 128,286,840 (GRCm39)	D573V	probably damaging	Het
Kcnj1	T	A	9: 32,308,056 (GRCm39)	L160Q	probably damaging	Het
Kcnmb3	A	T	3: 32,526,653 (GRCm39)	C179*	probably null	Het
Lrig1	A	G	6: 94,586,700 (GRCm39)	F659L	probably damaging	Het
Lrrc3	T	C	10: 77,737,253 (GRCm39)	D61G	probably benign	Het
Marchf4	T	G	1: 72,467,938 (GRCm39)	S365R	probably benign	Het
Mccc1	A	G	3: 36,051,703 (GRCm39)	L32S	probably benign	Het
Mei4	A	G	9: 81,772,216 (GRCm39)	T10A	probably benign	Het
Meis1	T	C	11: 18,959,222 (GRCm39)		probably benign	Het
Mllt1	T	C	17: 57,206,813 (GRCm39)	T344A	probably benign	Het
Mmp11	C	T	10: 75,761,419 (GRCm39)	A31T	probably damaging	Het
Mrps28	A	G	3: 8,947,614 (GRCm39)		probably benign	Het
Mthfsl	A	C	9: 88,597,550 (GRCm39)	L67V	probably damaging	Het
Ncaph2	A	G	15: 89,244,574 (GRCm39)	I11V	probably damaging	Het
Ncdn	G	A	4: 126,643,731 (GRCm39)	L364F	possibly damaging	Het
Nfat5	T	A	8: 108,051,284 (GRCm39)	D47E	probably damaging	Het
Or51g2	A	T	7: 102,622,614 (GRCm39)	I195N	possibly damaging	Het
Or5b106	T	A	19: 13,123,355 (GRCm39)	I223L	possibly damaging	Het
Or5bw2	C	T	7: 6,573,643 (GRCm39)	L218F	possibly damaging	Het
Pcdhb3	A	T	18: 37,435,452 (GRCm39)	I473F	probably damaging	Het
Pcdhgb8	A	T	18: 37,897,191 (GRCm39)	N754Y	probably damaging	Het
Pgbd5	C	A	8: 125,097,305 (GRCm39)	K408N	probably benign	Het
Pnisr	A	G	4: 21,859,330 (GRCm39)		probably benign	Het
Poldip3	A	T	15: 83,016,776 (GRCm39)		probably null	Het
Ppfia2	G	C	10: 106,597,978 (GRCm39)	L180F	probably damaging	Het
Psmc3	A	G	2: 90,896,317 (GRCm39)		probably benign	Het
Rab3gap1	T	A	1: 127,816,914 (GRCm39)	W58R	possibly damaging	Het
Rere	T	A	4: 150,703,601 (GRCm39)	W1528R	probably damaging	Het
Rhot1	A	G	11: 80,100,027 (GRCm39)		probably benign	Het
Rsf1	T	C	7: 97,311,612 (GRCm39)	S781P	probably damaging	Het
Rtraf	T	C	14: 19,873,784 (GRCm39)	K5R	probably damaging	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Ryr2	A	G	13: 11,609,872 (GRCm39)	V753A	probably damaging	Het
Ryr3	T	C	2: 112,661,530 (GRCm39)	H1820R	probably damaging	Het
Schip1	A	T	3: 68,315,818 (GRCm39)		probably benign	Het
Scin	T	A	12: 40,119,373 (GRCm39)	I552F	possibly damaging	Het
Selp	A	C	1: 163,972,475 (GRCm39)	T705P	probably damaging	Het
Sh3rf3	A	T	10: 58,842,925 (GRCm39)	D297V	probably damaging	Het
Slc24a4	T	A	12: 102,231,203 (GRCm39)		probably null	Het
Slc2a9	C	A	5: 38,574,603 (GRCm39)	L224F	probably benign	Het
Slc35e2	C	A	4: 155,700,693 (GRCm39)	P272Q	probably damaging	Het
Slf2	T	G	19: 44,960,100 (GRCm39)	D1022E	probably damaging	Het
Syne1	C	T	10: 5,007,909 (GRCm39)	C7932Y	probably damaging	Het
Tc2n	A	G	12: 101,631,954 (GRCm39)	L301P	probably damaging	Het
Tchhl1	A	G	3: 93,377,623 (GRCm39)	D109G	possibly damaging	Het
Thoc7	T	C	14: 13,953,154 (GRCm38)	I83V	probably damaging	Het
Tm4sf1	C	T	3: 57,200,448 (GRCm39)	G85S	probably damaging	Het
Tmem63a	T	A	1: 180,794,086 (GRCm39)	I541N	probably benign	Het
Tmpo	A	G	10: 90,985,411 (GRCm39)	V357A	probably damaging	Het
Unc80	T	C	1: 66,685,709 (GRCm39)	Y2278H	possibly damaging	Het
Vmn1r26	A	G	6: 57,985,808 (GRCm39)	F127S	probably damaging	Het
Vmn2r8	A	T	5: 108,945,264 (GRCm39)	M781K	probably damaging	Het
Vps36	T	G	8: 22,708,280 (GRCm39)	M348R	possibly damaging	Het
Vps45	T	C	3: 95,926,943 (GRCm39)	T535A	probably damaging	Het

Other mutations in Arhgef40

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Arhgef40	APN	14	52,226,417 (GRCm39)	missense	probably damaging	1.00
IGL00848:Arhgef40	APN	14	52,224,884 (GRCm39)	missense	probably damaging	1.00
IGL00966:Arhgef40	APN	14	52,229,155 (GRCm39)	critical splice donor site	probably null
IGL01123:Arhgef40	APN	14	52,231,803 (GRCm39)	missense	probably damaging	0.99
IGL02110:Arhgef40	APN	14	52,226,862 (GRCm39)	missense	probably damaging	1.00
IGL02490:Arhgef40	APN	14	52,226,652 (GRCm39)	missense	probably damaging	0.99
IGL02505:Arhgef40	APN	14	52,238,320 (GRCm39)	missense	probably damaging	1.00
IGL02636:Arhgef40	APN	14	52,234,865 (GRCm39)	missense	probably damaging	1.00
R0200:Arhgef40	UTSW	14	52,234,431 (GRCm39)	missense	probably damaging	0.99
R0496:Arhgef40	UTSW	14	52,242,364 (GRCm39)	unclassified	probably benign
R0608:Arhgef40	UTSW	14	52,234,431 (GRCm39)	missense	probably damaging	0.99
R0826:Arhgef40	UTSW	14	52,238,450 (GRCm39)	missense	probably benign	0.05
R1126:Arhgef40	UTSW	14	52,234,583 (GRCm39)	missense	probably damaging	0.96
R1330:Arhgef40	UTSW	14	52,227,613 (GRCm39)	missense	probably benign	0.42
R1612:Arhgef40	UTSW	14	52,241,538 (GRCm39)	missense	probably damaging	1.00
R1794:Arhgef40	UTSW	14	52,227,387 (GRCm39)	missense	possibly damaging	0.94
R1844:Arhgef40	UTSW	14	52,235,080 (GRCm39)	missense	probably damaging	0.99
R2018:Arhgef40	UTSW	14	52,241,162 (GRCm39)	missense	probably damaging	1.00
R2064:Arhgef40	UTSW	14	52,233,640 (GRCm39)	missense	probably damaging	1.00
R2321:Arhgef40	UTSW	14	52,231,733 (GRCm39)	splice site	probably benign
R3877:Arhgef40	UTSW	14	52,239,742 (GRCm39)	missense	probably damaging	1.00
R4233:Arhgef40	UTSW	14	52,227,628 (GRCm39)	missense	possibly damaging	0.50
R4596:Arhgef40	UTSW	14	52,224,681 (GRCm39)	critical splice donor site	probably null
R4676:Arhgef40	UTSW	14	52,228,416 (GRCm39)	nonsense	probably null
R4703:Arhgef40	UTSW	14	52,239,767 (GRCm39)	missense	probably damaging	1.00
R4704:Arhgef40	UTSW	14	52,239,767 (GRCm39)	missense	probably damaging	1.00
R4719:Arhgef40	UTSW	14	52,242,395 (GRCm39)	unclassified	probably benign
R4915:Arhgef40	UTSW	14	52,227,556 (GRCm39)	missense	probably damaging	1.00
R4918:Arhgef40	UTSW	14	52,227,556 (GRCm39)	missense	probably damaging	1.00
R5097:Arhgef40	UTSW	14	52,227,146 (GRCm39)	missense	probably damaging	1.00
R5183:Arhgef40	UTSW	14	52,241,556 (GRCm39)	missense	probably damaging	0.98
R5195:Arhgef40	UTSW	14	52,227,269 (GRCm39)	missense	possibly damaging	0.68
R5367:Arhgef40	UTSW	14	52,227,156 (GRCm39)	missense	probably damaging	0.99
R5381:Arhgef40	UTSW	14	52,229,306 (GRCm39)	missense	probably damaging	0.99
R5594:Arhgef40	UTSW	14	52,233,614 (GRCm39)	missense	probably damaging	1.00
R5632:Arhgef40	UTSW	14	52,231,795 (GRCm39)	missense	probably damaging	1.00
R5665:Arhgef40	UTSW	14	52,238,357 (GRCm39)	missense	possibly damaging	0.80
R5798:Arhgef40	UTSW	14	52,234,489 (GRCm39)	missense	probably damaging	1.00
R5820:Arhgef40	UTSW	14	52,224,953 (GRCm39)	missense	possibly damaging	0.76
R6229:Arhgef40	UTSW	14	52,227,547 (GRCm39)	missense	probably benign	0.06
R6451:Arhgef40	UTSW	14	52,238,456 (GRCm39)	missense	probably damaging	1.00
R6633:Arhgef40	UTSW	14	52,234,888 (GRCm39)	missense	probably damaging	1.00
R6642:Arhgef40	UTSW	14	52,228,419 (GRCm39)	unclassified	probably benign
R6675:Arhgef40	UTSW	14	52,229,098 (GRCm39)	missense	probably damaging	0.99
R6781:Arhgef40	UTSW	14	52,235,354 (GRCm39)	intron	probably benign
R6901:Arhgef40	UTSW	14	52,234,825 (GRCm39)	missense	probably damaging	1.00
R7852:Arhgef40	UTSW	14	52,229,254 (GRCm39)	missense	unknown
R7857:Arhgef40	UTSW	14	52,226,212 (GRCm39)	missense	probably damaging	0.97
R7914:Arhgef40	UTSW	14	52,225,032 (GRCm39)	missense	probably damaging	1.00
R8060:Arhgef40	UTSW	14	52,222,452 (GRCm39)	splice site	probably benign
R8144:Arhgef40	UTSW	14	52,235,632 (GRCm39)	missense	probably damaging	1.00
R8195:Arhgef40	UTSW	14	52,226,226 (GRCm39)	missense	probably damaging	1.00
R8432:Arhgef40	UTSW	14	52,226,857 (GRCm39)	missense	probably benign	0.00
R8738:Arhgef40	UTSW	14	52,238,414 (GRCm39)	missense	probably damaging	1.00
R8830:Arhgef40	UTSW	14	52,241,165 (GRCm39)	missense	probably damaging	1.00
R9038:Arhgef40	UTSW	14	52,235,072 (GRCm39)	missense	probably damaging	0.96
R9712:Arhgef40	UTSW	14	52,226,415 (GRCm39)	missense	probably damaging	0.99
U24488:Arhgef40	UTSW	14	52,235,673 (GRCm39)	missense	probably benign	0.07
X0023:Arhgef40	UTSW	14	52,241,141 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGTGTACGGCAGGTGAGAT -3'
(R):5'- CCCTACTGCTGGGCTACCA -3'

Sequencing Primer
(F):5'- TGAGATGGCAATGGGGTCC -3'
(R):5'- TGGGGTTACACATCCATACTAGGC -3'

Posted On

2016-04-15