Incidental Mutation 'R4921:Dusp8'
ID 378584
Institutional Source Beutler Lab
Gene Symbol Dusp8
Ensembl Gene ENSMUSG00000037887
Gene Name dual specificity phosphatase 8
Synonyms 5530400B01Rik, Nttp1
MMRRC Submission 042523-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.143) question?
Stock # R4921 (G1)
Quality Score 101
Status Not validated
Chromosome 7
Chromosomal Location 142079490-142095843 bp(-) (GRCm38)
Type of Mutation unclassified
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000114307 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000039926] [ENSMUST00000143661]
AlphaFold O09112
Predicted Effect probably benign
Transcript: ENSMUST00000039926
SMART Domains Protein: ENSMUSP00000049414
Gene: ENSMUSG00000037887

RHOD 13 135 4.71e-14 SMART
DSPc 160 299 3.6e-69 SMART
low complexity region 334 353 N/A INTRINSIC
low complexity region 360 371 N/A INTRINSIC
low complexity region 405 422 N/A INTRINSIC
low complexity region 427 449 N/A INTRINSIC
low complexity region 452 470 N/A INTRINSIC
low complexity region 488 512 N/A INTRINSIC
low complexity region 546 600 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000104221
Predicted Effect probably benign
Transcript: ENSMUST00000143661
SMART Domains Protein: ENSMUSP00000114307
Gene: ENSMUSG00000037887

RHOD 13 135 4.71e-14 SMART
Pfam:DSPc 168 231 1.5e-9 PFAM
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.1%
  • 10x: 95.7%
  • 20x: 90.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered myocardial fiber morphology, mildly increased cardiac muscle contractility at baseline, and decreased response of heart to induced stress. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 98 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik A T 5: 109,737,796 (GRCm38) N65K probably benign Het
Abcc9 T C 6: 142,590,436 (GRCm38) Y1524C probably benign Het
Acap1 A G 11: 69,887,193 (GRCm38) I102T probably damaging Het
Acvr2a T C 2: 48,893,541 (GRCm38) V284A possibly damaging Het
Adam3 T C 8: 24,684,614 (GRCm38) M712V probably benign Het
Adck1 T C 12: 88,441,138 (GRCm38) V213A probably benign Het
Adgrl3 G T 5: 81,512,110 (GRCm38) W242L probably damaging Het
Alk T C 17: 71,904,315 (GRCm38) T857A probably benign Het
Alms1 A G 6: 85,628,546 (GRCm38) T2393A probably benign Het
Ank3 C T 10: 70,002,109 (GRCm38) P240L probably damaging Het
Ankrd35 C A 3: 96,684,824 (GRCm38) L809M possibly damaging Het
Birc6 T A 17: 74,650,099 (GRCm38) L3690Q probably damaging Het
Bmp3 C A 5: 98,872,061 (GRCm38) F114L probably damaging Het
Cage1 G A 13: 38,019,208 (GRCm38) H627Y probably benign Het
Cars1 T C 7: 143,569,475 (GRCm38) D468G probably damaging Het
Ccdc148 T C 2: 58,829,802 (GRCm38) E487G probably damaging Het
Ccdc80 A T 16: 45,118,167 (GRCm38) I746F probably damaging Het
Ccl25 A G 8: 4,353,913 (GRCm38) Q119R possibly damaging Het
Cdh24 C T 14: 54,633,215 (GRCm38) D178N probably damaging Het
Cdk12 A T 11: 98,222,687 (GRCm38) T766S unknown Het
Chst15 T A 7: 132,247,884 (GRCm38) T443S probably benign Het
Cnbp T C 6: 87,845,146 (GRCm38) D125G possibly damaging Het
Cntn2 A T 1: 132,516,032 (GRCm38) V1003E possibly damaging Het
Crct1 A G 3: 93,014,825 (GRCm38) probably benign Het
Dand5 C T 8: 84,816,484 (GRCm38) C121Y probably damaging Het
Dmkn A T 7: 30,771,233 (GRCm38) D382V probably damaging Het
Dnase2b T A 3: 146,593,441 (GRCm38) T86S probably damaging Het
Eef1akmt1 C T 14: 57,550,632 (GRCm38) V90M probably damaging Het
Egfl7 G T 2: 26,590,980 (GRCm38) W168L probably benign Het
Ep400 A G 5: 110,665,810 (GRCm38) C2908R probably damaging Het
Espl1 A G 15: 102,315,241 (GRCm38) K1076E probably damaging Het
Exoc4 A G 6: 33,910,517 (GRCm38) N747D probably benign Het
Fancm T C 12: 65,077,141 (GRCm38) V191A probably benign Het
Fbxo17 A G 7: 28,732,789 (GRCm38) D97G probably benign Het
Fer1l6 G T 15: 58,600,311 (GRCm38) probably null Het
Flt4 T C 11: 49,627,143 (GRCm38) W337R probably damaging Het
Fpr-rs7 T C 17: 20,113,820 (GRCm38) H136R possibly damaging Het
Frem3 T A 8: 80,613,136 (GRCm38) I686N possibly damaging Het
Galnt9 A G 5: 110,577,449 (GRCm38) K84R probably damaging Het
Gfm2 T A 13: 97,175,676 (GRCm38) M760K probably damaging Het
Glis3 T C 19: 28,666,104 (GRCm38) T13A probably damaging Het
H2-K1 A G 17: 33,997,076 (GRCm38) V323A possibly damaging Het
Hbb-bs G A 7: 103,826,720 (GRCm38) A130V probably damaging Het
Herc2 T A 7: 56,229,690 (GRCm38) H4685Q probably benign Het
Ighv5-9-1 C T 12: 113,736,294 (GRCm38) R56H possibly damaging Het
Itgb4 A G 11: 116,006,605 (GRCm38) N1548S probably benign Het
Itpr3 T C 17: 27,098,005 (GRCm38) Y745H probably damaging Het
Kank3 G T 17: 33,817,200 (GRCm38) G14V probably damaging Het
Kif24 G T 4: 41,394,329 (GRCm38) S982Y probably damaging Het
Kifc5b C T 17: 26,921,023 (GRCm38) R53W probably damaging Het
Krt39 A G 11: 99,514,749 (GRCm38) S442P possibly damaging Het
Lcat G A 8: 105,942,442 (GRCm38) P67L possibly damaging Het
Maml2 T C 9: 13,621,175 (GRCm38) S562P probably damaging Het
Map3k8 G A 18: 4,349,124 (GRCm38) R65W possibly damaging Het
Mbd3 G T 10: 80,395,576 (GRCm38) R12S probably damaging Het
Msr1 T C 8: 39,624,251 (GRCm38) E106G possibly damaging Het
Myh4 T A 11: 67,254,028 (GRCm38) L1256Q probably damaging Het
Mypn G A 10: 63,147,936 (GRCm38) T511M possibly damaging Het
Nub1 A T 5: 24,701,469 (GRCm38) N331I probably benign Het
Nup107 A G 10: 117,770,511 (GRCm38) V440A possibly damaging Het
Ofcc1 A G 13: 40,214,517 (GRCm38) F174L probably benign Het
Or10d5 A C 9: 39,950,225 (GRCm38) V182G probably damaging Het
Or52ab2 A T 7: 103,320,543 (GRCm38) N44I probably damaging Het
Or9q2 T C 19: 13,795,465 (GRCm38) I49V probably benign Het
Parp2 C A 14: 50,819,268 (GRCm38) L310I probably damaging Het
Pcdh20 A G 14: 88,469,726 (GRCm38) V46A probably benign Het
Pcdhgb6 A G 18: 37,743,472 (GRCm38) D411G probably damaging Het
Pkd1l2 A T 8: 117,072,549 (GRCm38) N267K probably damaging Het
Pkd1l2 T C 8: 117,054,885 (GRCm38) E807G probably benign Het
Pramel24 A T 4: 143,728,326 (GRCm38) K398M possibly damaging Het
Rell1 C A 5: 63,936,033 (GRCm38) M126I probably damaging Het
Robo4 G A 9: 37,402,560 (GRCm38) E36K probably benign Het
Rpgrip1l T C 8: 91,261,009 (GRCm38) S807G probably benign Het
Rpl10a T C 17: 28,330,852 (GRCm38) V169A probably benign Het
Rubcn C T 16: 32,847,294 (GRCm38) V166I probably damaging Het
Sall2 T C 14: 52,315,393 (GRCm38) E113G possibly damaging Het
Sars2 T C 7: 28,752,438 (GRCm38) S423P possibly damaging Het
Scaper A G 9: 55,892,235 (GRCm38) I182T probably benign Het
Selp A G 1: 164,141,397 (GRCm38) D522G possibly damaging Het
Sema4b T A 7: 80,198,756 (GRCm38) I35N possibly damaging Het
Sgce A T 6: 4,694,153 (GRCm38) F268I probably damaging Het
Slc14a2 G T 18: 78,192,188 (GRCm38) A258E probably damaging Het
Slc17a9 T C 2: 180,735,949 (GRCm38) Y213H probably benign Het
Slc22a7 T G 17: 46,436,933 (GRCm38) I233L probably benign Het
Slc35f1 A G 10: 53,062,602 (GRCm38) Q210R probably damaging Het
Slc6a21 A G 7: 45,288,310 (GRCm38) E350G possibly damaging Het
Spata21 A T 4: 141,112,091 (GRCm38) D639V probably damaging Het
Svil A G 18: 5,108,631 (GRCm38) D1519G probably damaging Het
Tbccd1 T C 16: 22,841,899 (GRCm38) T56A probably benign Het
Tigit A T 16: 43,662,017 (GRCm38) I118N probably damaging Het
Tlr11 A T 14: 50,362,885 (GRCm38) Q776L possibly damaging Het
Tspan12 A C 6: 21,835,449 (GRCm38) I9S possibly damaging Het
Unc5c T A 3: 141,788,966 (GRCm38) Y347N probably damaging Het
Unk A G 11: 116,054,945 (GRCm38) T481A probably benign Het
Vmn1r192 A C 13: 22,187,480 (GRCm38) V190G probably damaging Het
Vnn3 A T 10: 23,864,575 (GRCm38) M259L probably benign Het
Zan T C 5: 137,408,370 (GRCm38) probably benign Het
Zdhhc6 T C 19: 55,313,210 (GRCm38) H113R probably damaging Het
Other mutations in Dusp8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01634:Dusp8 APN 7 142,084,423 (GRCm38) missense probably benign 0.05
IGL02458:Dusp8 APN 7 142,082,747 (GRCm38) missense probably benign 0.28
IGL02931:Dusp8 APN 7 142,082,930 (GRCm38) missense probably benign 0.00
IGL03329:Dusp8 APN 7 142,084,360 (GRCm38) nonsense probably null
R0009:Dusp8 UTSW 7 142,082,054 (GRCm38) unclassified probably benign
R1054:Dusp8 UTSW 7 142,082,067 (GRCm38) unclassified probably benign
R1611:Dusp8 UTSW 7 142,082,957 (GRCm38) missense probably benign 0.04
R1883:Dusp8 UTSW 7 142,084,348 (GRCm38) splice site probably null
R2119:Dusp8 UTSW 7 142,082,561 (GRCm38) missense possibly damaging 0.91
R2326:Dusp8 UTSW 7 142,090,063 (GRCm38) missense probably damaging 1.00
R2698:Dusp8 UTSW 7 142,081,964 (GRCm38) unclassified probably benign
R2905:Dusp8 UTSW 7 142,083,389 (GRCm38) nonsense probably null
R3849:Dusp8 UTSW 7 142,090,065 (GRCm38) missense probably damaging 1.00
R4942:Dusp8 UTSW 7 142,082,228 (GRCm38) missense possibly damaging 0.85
R5288:Dusp8 UTSW 7 142,089,993 (GRCm38) missense possibly damaging 0.95
R5385:Dusp8 UTSW 7 142,089,993 (GRCm38) missense possibly damaging 0.95
R5386:Dusp8 UTSW 7 142,089,993 (GRCm38) missense possibly damaging 0.95
R6301:Dusp8 UTSW 7 142,083,019 (GRCm38) splice site probably null
R6520:Dusp8 UTSW 7 142,083,681 (GRCm38) missense probably damaging 0.99
R6665:Dusp8 UTSW 7 142,090,105 (GRCm38) missense probably damaging 0.97
R9130:Dusp8 UTSW 7 142,088,418 (GRCm38) missense probably benign 0.12
RF016:Dusp8 UTSW 7 142,082,852 (GRCm38) missense probably benign 0.04
X0064:Dusp8 UTSW 7 142,082,027 (GRCm38) unclassified probably benign
Z1176:Dusp8 UTSW 7 142,090,077 (GRCm38) missense probably damaging 1.00
Z1176:Dusp8 UTSW 7 142,081,943 (GRCm38) missense unknown
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-04-15