Mutagenetix > Incidental Mutation

Incidental Mutation 'R4924:Tas2r103'

378860

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

042526-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R4924 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 133036198 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 302 (Y302H)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably benign
Transcript: ENSMUST00000032317
AA Change: Y302H

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: Y302H

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.0%
20x: 91.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	G	A	11: 120,011,525	H625Y	probably damaging	Het
Adamts4	C	T	1: 171,259,074	R812W	probably damaging	Het
Ajuba	A	C	14: 54,571,599		probably null	Het
Aox2	T	A	1: 58,305,344	V532D	probably damaging	Het
Arpc1b	G	A	5: 145,126,815	S295N	probably benign	Het
Baiap2	A	G	11: 119,997,024	S335G	probably damaging	Het
BC051142	C	T	17: 34,459,977	P221L	probably damaging	Het
Brap	T	G	5: 121,665,255	N155K	probably damaging	Het
Btn1a1	A	G	13: 23,464,226		probably benign	Het
Ccl12	G	A	11: 82,102,649	V38I	probably benign	Het
Cemip	T	C	7: 83,952,938	Y881C	probably damaging	Het
Cep128	T	C	12: 91,022,400		silent	Het
Chd5	A	C	4: 152,366,429	D670A	possibly damaging	Het
Cmip	T	C	8: 117,257,255	Y52H	probably benign	Het
Dmtn	A	G	14: 70,617,959	I30T	probably benign	Het
Dscaml1	T	A	9: 45,745,189	M1609K	probably damaging	Het
Edar	C	A	10: 58,629,375	E55D	probably damaging	Het
Ehmt1	A	T	2: 24,839,722	I601N	probably damaging	Het
Fam120a	A	T	13: 48,902,096	N705K	probably benign	Het
Gm21718	G	T	14: 51,312,835		noncoding transcript	Het
Gnaz	A	G	10: 74,991,713	D99G	probably benign	Het
Helz	G	A	11: 107,602,339	G196D	probably damaging	Het
Hif1a	T	C	12: 73,939,557	S341P	probably damaging	Het
Hivep1	G	T	13: 42,158,316	S1344I	probably benign	Het
Hspa1l	T	A	17: 34,977,856	Y290*	probably null	Het
Ift81	A	G	5: 122,594,616	L285S	possibly damaging	Het
Inpp4b	T	A	8: 82,122,624	N891K	probably damaging	Het
Irx6	C	T	8: 92,678,353	T283M	probably benign	Het
Kdm5b	A	G	1: 134,631,351	K1538E	probably benign	Het
Kif13a	A	G	13: 46,929,599	V8A	probably damaging	Het
Krtap15	T	A	16: 88,829,148	N34K	probably damaging	Het
Lama2	T	G	10: 27,369,141	I215L	probably damaging	Het
Ldlrad3	C	T	2: 102,069,983	R58H	possibly damaging	Het
Lvrn	C	T	18: 46,894,725	P869L	probably damaging	Het
Myo1d	A	G	11: 80,674,678	F411S	probably damaging	Het
Myo5b	T	A	18: 74,695,384	H702Q	probably benign	Het
Nat8f4	G	T	6: 85,901,419	Q41K	probably benign	Het
Nckap5	C	A	1: 126,027,028	E596*	probably null	Het
Nek10	T	A	14: 14,846,594		probably null	Het
Notch3	T	C	17: 32,144,731	Y1145C	probably damaging	Het
Nr4a2	A	T	2: 57,112,023	H76Q	probably benign	Het
Nup98	T	C	7: 102,134,978	Q1049R	probably damaging	Het
Olfr1080	A	T	2: 86,553,509	F205Y	probably damaging	Het
Olfr1298	A	T	2: 111,645,776	S74T	possibly damaging	Het
Olfr172	T	A	16: 58,760,619	R186*	probably null	Het
Pepd	T	C	7: 35,020,984	Y231H	probably benign	Het
Plagl1	G	T	10: 13,127,557	A190S	possibly damaging	Het
Plat	T	A	8: 22,778,253	I345N	probably damaging	Het
Pnmt	A	G	11: 98,387,460	E120G	probably damaging	Het
Prkrip1	C	A	5: 136,198,943		probably null	Het
Pygm	G	A	19: 6,393,724	A572T	probably damaging	Het
Rbm4b	T	C	19: 4,757,372	F39L	probably damaging	Het
Rpe65	A	G	3: 159,622,631	H388R	probably benign	Het
Scn4a	G	T	11: 106,320,088	A1701E	possibly damaging	Het
Sdk2	C	A	11: 113,857,758	W616L	probably damaging	Het
Serpina3m	T	A	12: 104,391,470	S218T	probably benign	Het
Siglece	C	T	7: 43,659,873	R87H	probably damaging	Het
Sim1	C	A	10: 50,909,902	L284M	probably damaging	Het
Smok3c	G	T	5: 138,065,582	E444*	probably null	Het
Snx4	T	C	16: 33,294,730	V427A	probably benign	Het
Srp68	A	T	11: 116,260,858	V304E	probably damaging	Het
Stag1	A	T	9: 100,796,755	H243L	possibly damaging	Het
Stx6	T	C	1: 155,173,991	V14A	probably damaging	Het
Sv2b	T	A	7: 75,136,421	Y417F	probably benign	Het
Thop1	T	C	10: 81,080,194	S404P	probably benign	Het
Trp53bp1	G	T	2: 121,221,220	C988*	probably null	Het
Ube3c	A	G	5: 29,631,271	E630G	possibly damaging	Het
Usf3	T	C	16: 44,217,355	S733P	probably benign	Het
Vmn1r195	A	G	13: 22,279,019	T220A	probably benign	Het
Vmn1r201	A	T	13: 22,474,712	H32L	probably benign	Het
Wnt2	A	G	6: 18,023,240	C137R	probably damaging	Het
Ythdc2	T	C	18: 44,847,804	S489P	probably damaging	Het
Zfp248	A	G	6: 118,429,072	C418R	probably damaging	Het
Zfp946	T	G	17: 22,455,521	F419V	probably damaging	Het
Zfp994	C	T	17: 22,200,757	E404K	probably damaging	Het
Zfpm1	T	C	8: 122,334,608	V304A	possibly damaging	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3977:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3980:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R6265:Tas2r103	UTSW	6	133036531	missense	probably damaging	1.00
R7768:Tas2r103	UTSW	6	133036849	missense	probably benign	0.06
R8851:Tas2r103	UTSW	6	133036933	missense
R9435:Tas2r103	UTSW	6	133036723	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATCTACAGTGTTGCCTGTCGAG -3'
(R):5'- ACTGTGGTAACCTTCCTGTTAC -3'

Sequencing Primer
(F):5'- TACAGTGTTGCCTGTCGAGTTACAG -3'
(R):5'- CGGAGTATAGGAGTAGCTTTT -3'

Posted On

2016-04-15