Mutagenetix > Incidental Mutation

Incidental Mutation 'R4907:Vmn2r109'

379141

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

042509-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R4907 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20540517-20564756 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20550086 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 547 (N547D)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably damaging
Transcript: ENSMUST00000167093
AA Change: N547D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: N547D

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.9%
20x: 91.1%

Validation Efficiency

98% (89/91)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700066M21Rik	G	T	1: 57,383,191 (GRCm38)	G242V	probably damaging	Het
4933412E24Rik	T	C	15: 60,016,108 (GRCm38)	E161G	probably benign	Het
Abcc5	A	G	16: 20,376,546 (GRCm38)	S734P	possibly damaging	Het
Actn1	T	G	12: 80,181,414 (GRCm38)	H394P	probably damaging	Het
Adam8	A	T	7: 139,989,373 (GRCm38)	D144E	probably benign	Het
Ankrd50	A	T	3: 38,456,675 (GRCm38)	N514K	probably damaging	Het
Ankrd50	T	A	3: 38,454,973 (GRCm38)	K1082*	probably null	Het
Atp4a	A	G	7: 30,719,092 (GRCm38)	I602V	possibly damaging	Het
Baz2a	A	G	10: 128,110,808 (GRCm38)	T64A	probably damaging	Het
Btbd19	A	G	4: 117,120,567 (GRCm38)		probably benign	Het
Casz1	T	A	4: 148,944,541 (GRCm38)	S1148T	probably damaging	Het
Ccdc18	T	A	5: 108,136,141 (GRCm38)	D103E	probably benign	Het
Cd209d	T	C	8: 3,877,948 (GRCm38)	N52S	probably benign	Het
Cdh7	T	A	1: 110,138,323 (GRCm38)	Y776N	probably damaging	Het
Cdk14	A	G	5: 5,249,140 (GRCm38)	V101A	probably damaging	Het
Cldn22	T	C	8: 47,824,707 (GRCm38)	V60A	probably benign	Het
Clec10a	G	A	11: 70,169,971 (GRCm38)	G183D	probably benign	Het
Cnga3	T	C	1: 37,241,942 (GRCm38)		probably null	Het
Cts3	A	T	13: 61,566,820 (GRCm38)	F224I	probably benign	Het
Fbxo40	A	G	16: 36,969,702 (GRCm38)	Y349H	probably benign	Het
Flt1	C	A	5: 147,683,939 (GRCm38)	A132S	probably benign	Het
Gm10029	A	T	13: 6,662,556 (GRCm38)		noncoding transcript	Het
Gm14399	T	A	2: 175,131,389 (GRCm38)		probably benign	Het
Gm9932	T	C	5: 100,199,155 (GRCm38)		noncoding transcript	Het
Immp1l	A	G	2: 105,937,117 (GRCm38)	Y107C	probably damaging	Het
Ism1	G	T	2: 139,678,752 (GRCm38)	G28V	probably benign	Het
Klf1	C	A	8: 84,903,185 (GRCm38)	S213*	probably null	Het
Krt12	T	C	11: 99,418,362 (GRCm38)	E329G	probably damaging	Het
Lama2	A	T	10: 27,164,946 (GRCm38)	N1460K	probably benign	Het
Llgl2	T	A	11: 115,853,974 (GRCm38)	Y932*	probably null	Het
Lrrc7	T	C	3: 158,161,240 (GRCm38)	I955V	probably damaging	Het
Ltbp1	A	G	17: 75,005,904 (GRCm38)	R7G	probably benign	Het
Mcm3ap	A	G	10: 76,493,441 (GRCm38)	E1152G	probably damaging	Het
Mgam2-ps	T	C	6: 40,834,743 (GRCm38)		noncoding transcript	Het
Mical3	T	A	6: 121,007,298 (GRCm38)	M206L	probably benign	Het
Mndal	T	G	1: 173,862,690 (GRCm38)	N358T	probably damaging	Het
Nckap5	A	G	1: 126,026,152 (GRCm38)	S824P	possibly damaging	Het
Ndufb2	T	C	6: 39,596,620 (GRCm38)		probably benign	Het
Nfatc3	C	A	8: 106,079,727 (GRCm38)	H401Q	probably damaging	Het
Npas2	T	C	1: 39,361,985 (GRCm38)	V810A	unknown	Het
Nt5c2	A	G	19: 46,896,539 (GRCm38)	V245A	possibly damaging	Het
Olfr1463	A	G	19: 13,234,793 (GRCm38)	D181G	probably damaging	Het
Olfr350	A	T	2: 36,850,258 (GRCm38)	I71F	probably benign	Het
Olfr615	A	G	7: 103,561,034 (GRCm38)	M186V	possibly damaging	Het
Olfr651	A	G	7: 104,553,311 (GRCm38)	R131G	probably damaging	Het
Olfr984	A	T	9: 40,100,659 (GRCm38)	M277K	probably benign	Het
Otof	A	T	5: 30,378,661 (GRCm38)		probably null	Het
Pck1	C	T	2: 173,157,023 (GRCm38)	T358I	probably damaging	Het
Pclo	T	C	5: 14,680,051 (GRCm38)		probably benign	Het
Pgm1	T	A	5: 64,103,878 (GRCm38)	F238L	probably benign	Het
Pgr	G	A	9: 8,947,043 (GRCm38)		probably benign	Het
Pkd1l3	T	A	8: 109,640,843 (GRCm38)	V1224E	probably damaging	Het
Pkd2l1	A	G	19: 44,154,142 (GRCm38)	V487A	possibly damaging	Het
Pkhd1	T	C	1: 20,209,226 (GRCm38)	D2956G	probably damaging	Het
Rexo2	A	T	9: 48,479,403 (GRCm38)		probably null	Het
Rgs22	T	A	15: 36,087,424 (GRCm38)	L573F	possibly damaging	Het
Rufy3	T	G	5: 88,584,192 (GRCm38)	I18S	possibly damaging	Het
Slc30a9	T	C	5: 67,346,162 (GRCm38)	L393P	probably damaging	Het
Snw1	T	C	12: 87,459,489 (GRCm38)	I214V	probably benign	Het
Sorbs1	G	A	19: 40,340,047 (GRCm38)	Q595*	probably null	Het
Spata21	T	A	4: 141,097,121 (GRCm38)		probably null	Het
Ss18l1	G	T	2: 180,063,399 (GRCm38)		probably null	Het
Tmem163	A	T	1: 127,519,370 (GRCm38)	L176H	probably damaging	Het
Tpd52	T	C	3: 8,944,608 (GRCm38)		probably null	Het
Tpsab1	T	A	17: 25,343,462 (GRCm38)	Y297F	possibly damaging	Het
Trim55	A	G	3: 19,674,374 (GRCm38)	T450A	probably benign	Het
Ttf1	C	T	2: 29,064,656 (GRCm38)	H11Y	possibly damaging	Het
Txndc11	A	T	16: 11,088,534 (GRCm38)	H377Q	probably benign	Het
Usp17la	A	G	7: 104,861,148 (GRCm38)	Y320C	probably damaging	Het
Vmn2r54	A	T	7: 12,616,223 (GRCm38)		probably null	Het
Wdr33	T	C	18: 31,906,993 (GRCm38)	*1331Q	probably null	Het
Wdr34	A	G	2: 30,032,460 (GRCm38)		probably null	Het
Wdr90	T	A	17: 25,860,650 (GRCm38)		probably benign	Het
Xpo7	T	C	14: 70,670,629 (GRCm38)	M903V	probably benign	Het
Zfhx3	T	A	8: 108,793,354 (GRCm38)	S369R	probably damaging	Het
Zfp808	T	C	13: 62,171,473 (GRCm38)	F172S	possibly damaging	Het
Zfp821	A	G	8: 109,723,993 (GRCm38)	E55G	probably benign	Het
Zfp964	T	C	8: 69,663,322 (GRCm38)	Y191H	possibly damaging	Het
Zfyve1	T	C	12: 83,574,872 (GRCm38)	T250A	probably damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,550,157 (GRCm38)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,541,121 (GRCm38)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,541,409 (GRCm38)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,554,392 (GRCm38)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,541,134 (GRCm38)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,541,080 (GRCm38)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,554,341 (GRCm38)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,554,160 (GRCm38)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,540,888 (GRCm38)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,540,984 (GRCm38)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,540,701 (GRCm38)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,554,256 (GRCm38)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,553,800 (GRCm38)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,541,250 (GRCm38)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,554,577 (GRCm38)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,541,074 (GRCm38)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,552,886 (GRCm38)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,540,675 (GRCm38)	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20,541,408 (GRCm38)	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20,554,580 (GRCm38)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,555,241 (GRCm38)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,540,740 (GRCm38)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,553,810 (GRCm38)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,564,707 (GRCm38)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,553,923 (GRCm38)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,541,186 (GRCm38)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,564,712 (GRCm38)	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20,554,536 (GRCm38)	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20,540,986 (GRCm38)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,554,442 (GRCm38)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,553,812 (GRCm38)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,553,024 (GRCm38)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,554,558 (GRCm38)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,541,394 (GRCm38)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,541,343 (GRCm38)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,553,891 (GRCm38)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,541,232 (GRCm38)	missense	probably benign	0.01
R5011:Vmn2r109	UTSW	17	20,555,189 (GRCm38)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,554,341 (GRCm38)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,540,927 (GRCm38)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,540,671 (GRCm38)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,540,519 (GRCm38)	makesense	probably null
R5702:Vmn2r109	UTSW	17	20,554,145 (GRCm38)	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20,554,305 (GRCm38)	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20,552,859 (GRCm38)	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20,541,056 (GRCm38)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,540,719 (GRCm38)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,541,178 (GRCm38)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,564,534 (GRCm38)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,554,523 (GRCm38)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,540,670 (GRCm38)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,540,711 (GRCm38)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,564,744 (GRCm38)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,540,963 (GRCm38)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,541,074 (GRCm38)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,540,683 (GRCm38)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,541,438 (GRCm38)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,540,781 (GRCm38)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,541,274 (GRCm38)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,554,403 (GRCm38)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,540,680 (GRCm38)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,552,855 (GRCm38)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,541,174 (GRCm38)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,540,520 (GRCm38)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,554,467 (GRCm38)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,564,707 (GRCm38)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,554,269 (GRCm38)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,555,070 (GRCm38)	missense
Z1176:Vmn2r109	UTSW	17	20,552,994 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATGGTCCATTTCAAAGAGATGC -3'
(R):5'- CCTTAGATACATTTGTTTCTCCAGG -3'

Sequencing Primer
(F):5'- TCAGACCATTTTCATTGCC -3'
(R):5'- ACATTTGTTTCTCCAGGACATAATC -3'

Posted On

2016-04-15