Mutagenetix > Incidental Mutation

Incidental Mutation 'R4911:Rif1'

379478

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

042513-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4911 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 52110518 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 1328 (T1328I)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000069794
AA Change: T1328I

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: T1328I

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000112693
AA Change: T1328I

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: T1328I

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125322

Predicted Effect

probably benign
Transcript: ENSMUST00000125376

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Meta Mutation Damage Score

0.2057

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 89.7%

Validation Efficiency

97% (149/153)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 132 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010111I01Rik	A	T	13: 63,170,939		probably null	Het
4430402I18Rik	G	A	19: 28,897,503		probably benign	Het
Aacs	A	G	5: 125,506,160	D260G	possibly damaging	Het
Aadacl4	T	A	4: 144,613,792	L29H	probably damaging	Het
Abca7	T	C	10: 80,012,188		probably null	Het
Adgre1	G	A	17: 57,447,832	M643I	possibly damaging	Het
Adgrl2	A	T	3: 148,890,463	M79K	probably damaging	Het
Ankrd55	T	A	13: 112,323,039		probably null	Het
Arhgap21	A	G	2: 20,858,989	I1257T	probably damaging	Het
Arhgap26	T	A	18: 38,993,637		probably benign	Het
Arhgap39	A	G	15: 76,737,805	S199P	probably damaging	Het
Arhgef5	A	G	6: 43,272,828	D171G	probably benign	Het
Atp7a	T	C	X: 106,120,374	V1305A	probably damaging	Het
B3gnt3	T	A	8: 71,692,934	R263S	probably benign	Het
B9d1	A	C	11: 61,507,671	D59A	probably benign	Het
Bag2	A	T	1: 33,748,276	I55N	probably benign	Het
Bank1	T	C	3: 136,284,243	I29V	probably benign	Het
Bbs2	A	T	8: 94,089,115	D174E	probably damaging	Het
Bmp8b	T	C	4: 123,115,237	W203R	probably damaging	Het
Cacnb2	A	T	2: 14,981,340	E359D	possibly damaging	Het
Camp	T	A	9: 109,847,583		probably null	Het
Casp4	C	T	9: 5,328,580		probably benign	Het
Ccdc110	A	T	8: 45,942,907	T612S	probably benign	Het
Cd209b	T	A	8: 3,926,640		probably null	Het
Cemip	T	C	7: 83,983,253	D367G	probably damaging	Het
Cers5	T	A	15: 99,747,079	N131I	probably damaging	Het
Chd5	T	G	4: 152,360,672	V370G	probably damaging	Het
Cipc	C	A	12: 86,952,757	Q20K	probably benign	Het
Cnr2	T	A	4: 135,917,201	F197I	possibly damaging	Het
Col7a1	G	A	9: 108,975,219	G2233S	unknown	Het
Dcdc2b	T	A	4: 129,611,267	I125F	possibly damaging	Het
Ddi2	A	G	4: 141,684,402	S400P	probably benign	Het
Ddx31	C	A	2: 28,904,684	T664K	probably benign	Het
Dhx30	C	A	9: 110,100,924	G35C	probably damaging	Het
Dmxl2	G	A	9: 54,411,653	T1576I	probably damaging	Het
Dnah1	T	C	14: 31,295,323	Y1510C	probably damaging	Het
Dnah2	C	T	11: 69,499,104		probably null	Het
Dock10	C	A	1: 80,606,236	G163C	probably damaging	Het
Edar	T	C	10: 58,621,324	N144S	probably benign	Het
Elavl2	T	A	4: 91,308,678	E54D	possibly damaging	Het
Enam	T	G	5: 88,502,314	S561A	probably benign	Het
Ercc5	C	A	1: 44,166,871	H315N	possibly damaging	Het
Esp31	A	G	17: 38,644,661	E65G	possibly damaging	Het
Faap100	T	C	11: 120,372,113	I806M	probably benign	Het
Fam159a	T	C	4: 108,377,461	T70A	probably benign	Het
Fbxw21	T	C	9: 109,145,663	Y263C	probably damaging	Het
Fsip2	T	A	2: 82,981,493	S2719T	possibly damaging	Het
Fv1	T	C	4: 147,869,418	V147A	probably benign	Het
Gal	T	C	19: 3,411,590	E65G	probably benign	Het
Galnt6	T	C	15: 100,716,178	T81A	probably benign	Het
Gcc2	A	G	10: 58,270,439	E399G	probably damaging	Het
Gfi1b	A	T	2: 28,610,102	C306S	probably damaging	Het
Gm10654	T	A	8: 70,931,852		noncoding transcript	Het
Gm20388	A	G	8: 123,080,604		probably benign	Het
Gm4788	T	G	1: 139,774,563	D61A	probably damaging	Het
Gm4953	T	A	1: 159,168,359		noncoding transcript	Het
Gorasp2	T	C	2: 70,688,339		probably benign	Het
Gse1	T	C	8: 120,568,466		probably benign	Het
H2-D1	A	G	17: 35,265,997	E278G	probably damaging	Het
Herc2	T	C	7: 56,227,892	L4569P	probably damaging	Het
Hgs	T	A	11: 120,477,202	S246T	probably damaging	Het
Hs2st1	T	C	3: 144,465,082	T110A	probably benign	Het
Hydin	A	G	8: 110,595,438	Y4574C	probably benign	Het
Ighv1-23	C	A	12: 114,764,752	V17F	possibly damaging	Het
Ighv8-14	A	T	12: 115,808,565		noncoding transcript	Het
Igsf21	C	T	4: 140,034,623	R248H	probably benign	Het
Il23r	T	C	6: 67,423,561	N595S	probably benign	Het
Il5ra	G	T	6: 106,715,668	P372Q	probably damaging	Het
Iltifb	T	C	10: 118,294,989	M1V	probably null	Het
Inppl1	A	G	7: 101,832,309	V222A	possibly damaging	Het
Ints10	T	C	8: 68,827,312	V697A	probably damaging	Het
Isg15	T	A	4: 156,199,760	T104S	probably benign	Het
Kif11	A	G	19: 37,417,937	T983A	probably benign	Het
Kif3b	A	T	2: 153,317,292	K338*	probably null	Het
Kpna2	T	A	11: 106,990,719	I362F	probably damaging	Het
Lama2	G	A	10: 27,138,927	T1595M	probably damaging	Het
Lrrc36	A	G	8: 105,426,935	T126A	probably benign	Het
Lrrk1	T	A	7: 66,295,454	D604V	probably damaging	Het
Map7d1	T	C	4: 126,236,691	K409E	probably damaging	Het
Mast2	T	C	4: 116,353,057	T110A	probably benign	Het
Micall2	G	A	5: 139,716,825	T221M	probably damaging	Het
Morc2b	T	A	17: 33,137,377	N474Y	probably damaging	Het
Myo1e	T	A	9: 70,343,096	M528K	probably benign	Het
Nlrp1c-ps	G	T	11: 71,260,369		noncoding transcript	Het
Nostrin	T	C	2: 69,161,142	S160P	possibly damaging	Het
Nup133	T	C	8: 123,927,131	R530G	possibly damaging	Het
Olfr452	T	A	6: 42,790,204	L55H	probably damaging	Het
Olfr488	T	C	7: 108,256,037	I34V	possibly damaging	Het
Olfr894	A	G	9: 38,219,096	E91G	probably damaging	Het
Pabpc4l	A	T	3: 46,446,162	M349K	possibly damaging	Het
Pah	G	A	10: 87,570,267	G256S	probably benign	Het
Park2	G	T	17: 10,840,472		probably benign	Het
Pigk	T	C	3: 152,740,204	S151P	probably damaging	Het
Plcd4	A	C	1: 74,564,413	T658P	possibly damaging	Het
Pld4	A	T	12: 112,764,517	S178C	probably benign	Het
Polr1a	G	A	6: 71,909,229	E23K	possibly damaging	Het
Pomt1	T	C	2: 32,241,679	S127P	probably damaging	Het
Pon2	A	G	6: 5,269,029	V215A	possibly damaging	Het
Ppp3cb	A	T	14: 20,509,440	M416K	probably damaging	Het
Prdm1	A	T	10: 44,442,052	N273K	possibly damaging	Het
Prg4	T	A	1: 150,455,823		probably benign	Het
Ptch1	G	A	13: 63,523,052	T888M	probably damaging	Het
Pusl1	T	G	4: 155,891,442		probably benign	Het
Rapgef6	A	T	11: 54,622,317	E122D	probably damaging	Het
Rngtt	T	G	4: 33,500,292		probably null	Het
Runx2	G	A	17: 44,814,192	P80L	probably damaging	Het
Samd1	A	G	8: 83,998,989		probably benign	Het
Scamp5	A	T	9: 57,451,452	F14I	probably damaging	Het
Shank3	C	T	15: 89,504,344	R380C	probably damaging	Het
Slc1a4	C	A	11: 20,332,166	A103S	probably damaging	Het
Slc39a14	A	G	14: 70,309,922	V325A	probably benign	Het
Slc5a9	C	A	4: 111,891,744		probably null	Het
Spta1	T	A	1: 174,185,647	S375T	probably damaging	Het
Sval1	A	G	6: 41,955,444	N76S	probably benign	Het
Synpo	C	T	18: 60,629,864		probably benign	Het
Sytl5	C	T	X: 9,915,602	P181L	possibly damaging	Het
Tacc1	A	G	8: 25,182,606	M111T	possibly damaging	Het
Tec	T	C	5: 72,756,351	D613G	probably benign	Het
Tecpr2	C	T	12: 110,931,487	T391I	possibly damaging	Het
Thop1	G	A	10: 81,073,291	G52D	probably damaging	Het
Tmco5	T	A	2: 116,892,208	V270D	possibly damaging	Het
Ttn	C	T	2: 76,726,629	E28265K	possibly damaging	Het
Tuft1	T	C	3: 94,635,443	D72G	probably damaging	Het
Tyrp1	C	T	4: 80,850,907		probably benign	Het
Usp9y	T	C	Y: 1,308,041	D2265G	probably damaging	Het
Vmn2r104	G	A	17: 20,030,026	A661V	probably benign	Het
Vwa5a	A	T	9: 38,737,972	N672I	probably benign	Het
Wdr95	A	T	5: 149,611,692	K772*	probably null	Het
Ybx1	T	C	4: 119,282,813	T106A	probably benign	Het
Ypel3	T	C	7: 126,777,789	S14P	probably benign	Het
Zfp518a	T	A	19: 40,915,528	S1300R	probably benign	Het
Zscan5b	A	T	7: 6,239,190	*469Y	probably null	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52121007	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52111070	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52119117	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52085140	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52095948	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52111303	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52111522	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52112543	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52105515	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52116696	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52077065	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52093576	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52110125	nonsense	probably null
IGL03060:Rif1	APN	2	52112137	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52103622	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52090261	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52076988	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52112599	missense	probably benign	0.32
hifi	UTSW	2	52110324	unclassified	probably benign
nietzsche	UTSW	2	52077020	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52111958	missense
R0017:Rif1	UTSW	2	52116674	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52116674	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52111117	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52111117	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52110092	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52090286	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52110324	unclassified	probably benign
R0278:Rif1	UTSW	2	52110324	unclassified	probably benign
R0288:Rif1	UTSW	2	52110013	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52110324	unclassified	probably benign
R0345:Rif1	UTSW	2	52110324	unclassified	probably benign
R0346:Rif1	UTSW	2	52110324	unclassified	probably benign
R0383:Rif1	UTSW	2	52085141	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52110324	unclassified	probably benign
R0387:Rif1	UTSW	2	52110324	unclassified	probably benign
R0388:Rif1	UTSW	2	52110324	unclassified	probably benign
R0456:Rif1	UTSW	2	52110324	unclassified	probably benign
R0477:Rif1	UTSW	2	52110324	unclassified	probably benign
R0505:Rif1	UTSW	2	52110737	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52110324	unclassified	probably benign
R0511:Rif1	UTSW	2	52110324	unclassified	probably benign
R0512:Rif1	UTSW	2	52110324	unclassified	probably benign
R0633:Rif1	UTSW	2	52112563	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52110324	unclassified	probably benign
R0638:Rif1	UTSW	2	52111588	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52110324	unclassified	probably benign
R0675:Rif1	UTSW	2	52110324	unclassified	probably benign
R0707:Rif1	UTSW	2	52110324	unclassified	probably benign
R0726:Rif1	UTSW	2	52110353	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52110324	unclassified	probably benign
R0744:Rif1	UTSW	2	52110324	unclassified	probably benign
R0938:Rif1	UTSW	2	52110324	unclassified	probably benign
R0939:Rif1	UTSW	2	52110324	unclassified	probably benign
R0940:Rif1	UTSW	2	52110324	unclassified	probably benign
R0941:Rif1	UTSW	2	52110324	unclassified	probably benign
R0942:Rif1	UTSW	2	52110324	unclassified	probably benign
R0943:Rif1	UTSW	2	52110324	unclassified	probably benign
R1006:Rif1	UTSW	2	52085029	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52111562	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52110324	unclassified	probably benign
R1175:Rif1	UTSW	2	52107628	unclassified	probably benign
R1183:Rif1	UTSW	2	52110324	unclassified	probably benign
R1184:Rif1	UTSW	2	52110324	unclassified	probably benign
R1271:Rif1	UTSW	2	52110324	unclassified	probably benign
R1332:Rif1	UTSW	2	52078314	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52078314	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52111555	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52110324	unclassified	probably benign
R1527:Rif1	UTSW	2	52110324	unclassified	probably benign
R1560:Rif1	UTSW	2	52111131	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52073223	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52110324	unclassified	probably benign
R1625:Rif1	UTSW	2	52103640	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52110324	unclassified	probably benign
R1689:Rif1	UTSW	2	52110324	unclassified	probably benign
R1731:Rif1	UTSW	2	52110324	unclassified	probably benign
R1744:Rif1	UTSW	2	52112392	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52110324	unclassified	probably benign
R1748:Rif1	UTSW	2	52110324	unclassified	probably benign
R1831:Rif1	UTSW	2	52078495	nonsense	probably null
R1902:Rif1	UTSW	2	52116673	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52098409	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52110324	unclassified	probably benign
R2000:Rif1	UTSW	2	52081298	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52092346	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52093576	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52110324	unclassified	probably benign
R2109:Rif1	UTSW	2	52110324	unclassified	probably benign
R2125:Rif1	UTSW	2	52110324	unclassified	probably benign
R2126:Rif1	UTSW	2	52110324	unclassified	probably benign
R2145:Rif1	UTSW	2	52111400	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52110324	unclassified	probably benign
R2153:Rif1	UTSW	2	52110324	unclassified	probably benign
R2213:Rif1	UTSW	2	52110324	unclassified	probably benign
R2327:Rif1	UTSW	2	52110324	unclassified	probably benign
R2512:Rif1	UTSW	2	52110324	unclassified	probably benign
R2513:Rif1	UTSW	2	52110324	unclassified	probably benign
R2516:Rif1	UTSW	2	52110324	unclassified	probably benign
R2520:Rif1	UTSW	2	52110324	unclassified	probably benign
R2905:Rif1	UTSW	2	52098504	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52082764	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52110324	unclassified	probably benign
R3156:Rif1	UTSW	2	52110324	unclassified	probably benign
R3429:Rif1	UTSW	2	52110324	unclassified	probably benign
R3707:Rif1	UTSW	2	52093580	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52112545	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52116747	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52121087	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52098471	nonsense	probably null
R4668:Rif1	UTSW	2	52111952	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52106942	missense	probably null	1.00
R4715:Rif1	UTSW	2	52073139	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52098934	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52112747	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52112747	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52093611	intron	probably benign
R4951:Rif1	UTSW	2	52084986	splice site	probably null
R5044:Rif1	UTSW	2	52109928	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52092295	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52120309	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52081289	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52077020	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52111824	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52120971	intron	probably benign
R5476:Rif1	UTSW	2	52089595	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52098916	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52121158	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52105639	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52085053	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52119156	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52107669	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52078263	splice site	probably null
R6928:Rif1	UTSW	2	52095961	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52112691	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52076989	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52105619	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52078507	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52076175	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52088654	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52085141	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52116356	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52078387	nonsense	probably null
R7962:Rif1	UTSW	2	52074276	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52090278	missense	noncoding transcript
R8390:Rif1	UTSW	2	52110923	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52112551	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52110999	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52111730	missense
R8785:Rif1	UTSW	2	52110481	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52098863	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52110977	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52111850	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52108552	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52111139	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52120344	missense
R9477:Rif1	UTSW	2	52111330	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52081299	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52110454	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52089595	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52074315	missense	probably benign	0.00
X0064:Rif1	UTSW	2	52094633	missense	probably damaging	0.96
Z1177:Rif1	UTSW	2	52088648	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGTTACAGTGAGAAATAACCAGG -3'
(R):5'- TCGTGCTACAACTGAGGCTG -3'

Sequencing Primer
(F):5'- GGATAACACAACTAACACTGACATG -3'
(R):5'- GTGCTACAACTGAGGCTGTACTTTC -3'

Posted On

2016-04-15