Mutagenetix > Incidental Mutation

Incidental Mutation 'R4911:Dhx30'

379550

Institutional Source

Beutler Lab

Gene Symbol

Dhx30

Ensembl Gene

ENSMUSG00000032480

Gene Name

DExH-box helicase 30

Synonyms

2810477H02Rik, helG, C130058C04Rik, Ddx30, DEAH (Asp-Glu-Ala-His) box polypeptide 30

MMRRC Submission

042513-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4911 (G1)

Quality Score

157

Status

Validated

Chromosome

Chromosomal Location

109913387-109946728 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 109929992 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 35 (G35C)

Ref Sequence

ENSEMBL: ENSMUSP00000142836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062368] [ENSMUST00000111991] [ENSMUST00000165596] [ENSMUST00000196171] [ENSMUST00000196455] [ENSMUST00000196497] [ENSMUST00000197928] [ENSMUST00000198443] [ENSMUST00000198425] [ENSMUST00000199835] [ENSMUST00000199529] [ENSMUST00000200476] [ENSMUST00000200066]

AlphaFold

Q99PU8

Predicted Effect

probably benign
Transcript: ENSMUST00000062368
AA Change: G35C

PolyPhen 2 Score 0.113 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000062622
Gene: ENSMUSG00000032480
AA Change: G35C

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	48	64	N/A	INTRINSIC
internal_repeat_1	76	123	2.53e-5	PROSPERO
low complexity region	217	228	N/A	INTRINSIC
internal_repeat_1	268	314	2.53e-5	PROSPERO
low complexity region	321	342	N/A	INTRINSIC
DEXDc	461	650	9.66e-29	SMART
low complexity region	679	689	N/A	INTRINSIC
HELICc	711	816	1.63e-17	SMART
HA2	879	969	5.16e-22	SMART
Pfam:OB_NTP_bind	984	1134	5.7e-9	PFAM
low complexity region	1200	1208	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000111991

SMART Domains

Protein: ENSMUSP00000107622
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	47	94	6.21e-5	PROSPERO
low complexity region	188	199	N/A	INTRINSIC
internal_repeat_1	239	285	6.21e-5	PROSPERO
low complexity region	292	313	N/A	INTRINSIC
DEXDc	432	621	9.66e-29	SMART
low complexity region	650	660	N/A	INTRINSIC
HELICc	682	787	1.63e-17	SMART
HA2	850	940	5.16e-22	SMART
Pfam:OB_NTP_bind	952	1106	2.1e-7	PFAM
low complexity region	1171	1179	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000165596

SMART Domains

Protein: ENSMUSP00000129174
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	70	117	6.77e-5	PROSPERO
low complexity region	211	222	N/A	INTRINSIC
internal_repeat_1	262	308	6.77e-5	PROSPERO
low complexity region	315	336	N/A	INTRINSIC
DEXDc	455	644	9.66e-29	SMART
low complexity region	673	683	N/A	INTRINSIC
HELICc	705	810	1.63e-17	SMART
HA2	873	963	5.16e-22	SMART
Pfam:OB_NTP_bind	975	1129	1.8e-7	PFAM
low complexity region	1194	1202	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000196171

SMART Domains

Protein: ENSMUSP00000143616
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	39	86	5.84e-5	PROSPERO
low complexity region	180	191	N/A	INTRINSIC
internal_repeat_1	231	277	5.84e-5	PROSPERO
low complexity region	284	305	N/A	INTRINSIC
DEXDc	424	613	9.66e-29	SMART
low complexity region	642	652	N/A	INTRINSIC
HELICc	674	779	1.63e-17	SMART
HA2	842	932	5.16e-22	SMART
Pfam:OB_NTP_bind	947	1097	2.8e-9	PFAM
low complexity region	1163	1171	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000196455
AA Change: G39C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000143751
Gene: ENSMUSG00000032480
AA Change: G39C

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	52	68	N/A	INTRINSIC
PDB:2DB2\|A	77	113	3e-19	PDB
low complexity region	114	125	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000196497

SMART Domains

Protein: ENSMUSP00000143529
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
PDB:2DB2\|A	67	101	5e-18	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000197928

SMART Domains

Protein: ENSMUSP00000142549
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	47	94	6.21e-5	PROSPERO
low complexity region	188	199	N/A	INTRINSIC
internal_repeat_1	239	285	6.21e-5	PROSPERO
low complexity region	292	313	N/A	INTRINSIC
DEXDc	432	621	9.66e-29	SMART
low complexity region	650	660	N/A	INTRINSIC
HELICc	682	787	1.63e-17	SMART
HA2	850	940	5.16e-22	SMART
Pfam:OB_NTP_bind	952	1106	2.1e-7	PFAM
low complexity region	1171	1179	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000198443
AA Change: G35C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000142836
Gene: ENSMUSG00000032480
AA Change: G35C

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	48	64	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200029

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000198026

Predicted Effect

probably benign
Transcript: ENSMUST00000198425

SMART Domains

Protein: ENSMUSP00000142659
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	70	117	6.77e-5	PROSPERO
low complexity region	211	222	N/A	INTRINSIC
internal_repeat_1	262	308	6.77e-5	PROSPERO
low complexity region	315	336	N/A	INTRINSIC
DEXDc	455	644	9.66e-29	SMART
low complexity region	673	683	N/A	INTRINSIC
HELICc	705	810	1.63e-17	SMART
HA2	873	963	5.16e-22	SMART
Pfam:OB_NTP_bind	975	1129	1.8e-7	PFAM
low complexity region	1194	1202	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000199835

SMART Domains

Protein: ENSMUSP00000142636
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
PDB:2DB2\|A	67	171	3e-68	PDB
SCOP:d1di2a_	76	145	3e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000199529

SMART Domains

Protein: ENSMUSP00000142489
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	47	94	6.21e-5	PROSPERO
low complexity region	188	199	N/A	INTRINSIC
internal_repeat_1	239	285	6.21e-5	PROSPERO
low complexity region	292	313	N/A	INTRINSIC
DEXDc	432	621	9.66e-29	SMART
low complexity region	650	660	N/A	INTRINSIC
HELICc	682	787	1.63e-17	SMART
HA2	850	940	5.16e-22	SMART
Pfam:OB_NTP_bind	952	1106	2.1e-7	PFAM
low complexity region	1171	1179	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000200476

Predicted Effect

probably benign
Transcript: ENSMUST00000200066

SMART Domains

Protein: ENSMUSP00000143371
Gene: ENSMUSG00000032480

Domain	Start	End	E-Value	Type
internal_repeat_1	47	94	6.21e-5	PROSPERO
low complexity region	188	199	N/A	INTRINSIC
internal_repeat_1	239	285	6.21e-5	PROSPERO
low complexity region	292	313	N/A	INTRINSIC
DEXDc	432	621	9.66e-29	SMART
low complexity region	650	660	N/A	INTRINSIC
HELICc	682	787	1.63e-17	SMART
HA2	850	940	5.16e-22	SMART
Pfam:OB_NTP_bind	952	1106	2.1e-7	PFAM
low complexity region	1171	1179	N/A	INTRINSIC

Meta Mutation Damage Score

0.1417

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 89.7%

Validation Efficiency

97% (149/153)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
PHENOTYPE: Mice homozygous for a gene trapped allele exhibit complete embryonic lethality associated with embryonic growth retardation, failure of initiation of embryo turning, and absence of organized somites and neural tube formation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 132 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aacs	A	G	5: 125,583,224 (GRCm39)	D260G	possibly damaging	Het
Aadacl4	T	A	4: 144,340,362 (GRCm39)	L29H	probably damaging	Het
Abca7	T	C	10: 79,848,022 (GRCm39)		probably null	Het
Adgre1	G	A	17: 57,754,832 (GRCm39)	M643I	possibly damaging	Het
Adgrl2	A	T	3: 148,596,099 (GRCm39)	M79K	probably damaging	Het
Ankrd55	T	A	13: 112,459,573 (GRCm39)		probably null	Het
Aopep	A	T	13: 63,318,753 (GRCm39)		probably null	Het
Arhgap21	A	G	2: 20,863,800 (GRCm39)	I1257T	probably damaging	Het
Arhgap26	T	A	18: 39,126,690 (GRCm39)		probably benign	Het
Arhgap39	A	G	15: 76,622,005 (GRCm39)	S199P	probably damaging	Het
Arhgef5	A	G	6: 43,249,762 (GRCm39)	D171G	probably benign	Het
Atp7a	T	C	X: 105,163,980 (GRCm39)	V1305A	probably damaging	Het
B3gnt3	T	A	8: 72,145,578 (GRCm39)	R263S	probably benign	Het
B9d1	A	C	11: 61,398,497 (GRCm39)	D59A	probably benign	Het
Bag2	A	T	1: 33,787,357 (GRCm39)	I55N	probably benign	Het
Bank1	T	C	3: 135,990,004 (GRCm39)	I29V	probably benign	Het
Bbs2	A	T	8: 94,815,743 (GRCm39)	D174E	probably damaging	Het
Bmp8b	T	C	4: 123,009,030 (GRCm39)	W203R	probably damaging	Het
Cacnb2	A	T	2: 14,986,151 (GRCm39)	E359D	possibly damaging	Het
Camp	T	A	9: 109,676,651 (GRCm39)		probably null	Het
Casp4	C	T	9: 5,328,580 (GRCm39)		probably benign	Het
Ccdc110	A	T	8: 46,395,944 (GRCm39)	T612S	probably benign	Het
Cd209b	T	A	8: 3,976,640 (GRCm39)		probably null	Het
Cemip	T	C	7: 83,632,461 (GRCm39)	D367G	probably damaging	Het
Cers5	T	A	15: 99,644,960 (GRCm39)	N131I	probably damaging	Het
Cfhr4	T	G	1: 139,702,301 (GRCm39)	D61A	probably damaging	Het
Chd5	T	G	4: 152,445,129 (GRCm39)	V370G	probably damaging	Het
Cipc	C	A	12: 86,999,531 (GRCm39)	Q20K	probably benign	Het
Cnr2	T	A	4: 135,644,512 (GRCm39)	F197I	possibly damaging	Het
Col7a1	G	A	9: 108,804,287 (GRCm39)	G2233S	unknown	Het
Dcdc2b	T	A	4: 129,505,060 (GRCm39)	I125F	possibly damaging	Het
Ddi2	A	G	4: 141,411,713 (GRCm39)	S400P	probably benign	Het
Ddx31	C	A	2: 28,794,696 (GRCm39)	T664K	probably benign	Het
Dmxl2	G	A	9: 54,318,937 (GRCm39)	T1576I	probably damaging	Het
Dnah1	T	C	14: 31,017,280 (GRCm39)	Y1510C	probably damaging	Het
Dnah2	C	T	11: 69,389,930 (GRCm39)		probably null	Het
Dock10	C	A	1: 80,583,953 (GRCm39)	G163C	probably damaging	Het
Edar	T	C	10: 58,457,146 (GRCm39)	N144S	probably benign	Het
Elavl2	T	A	4: 91,196,915 (GRCm39)	E54D	possibly damaging	Het
Enam	T	G	5: 88,650,173 (GRCm39)	S561A	probably benign	Het
Ercc5	C	A	1: 44,206,031 (GRCm39)	H315N	possibly damaging	Het
Esp31	A	G	17: 38,955,552 (GRCm39)	E65G	possibly damaging	Het
Faap100	T	C	11: 120,262,939 (GRCm39)	I806M	probably benign	Het
Fbxw21	T	C	9: 108,974,731 (GRCm39)	Y263C	probably damaging	Het
Fsip2	T	A	2: 82,811,837 (GRCm39)	S2719T	possibly damaging	Het
Fv1	T	C	4: 147,953,875 (GRCm39)	V147A	probably benign	Het
Gal	T	C	19: 3,461,590 (GRCm39)	E65G	probably benign	Het
Galnt2l	A	G	8: 123,807,343 (GRCm39)		probably benign	Het
Galnt6	T	C	15: 100,614,059 (GRCm39)	T81A	probably benign	Het
Gcc2	A	G	10: 58,106,261 (GRCm39)	E399G	probably damaging	Het
Gfi1b	A	T	2: 28,500,114 (GRCm39)	C306S	probably damaging	Het
Gm10654	T	A	8: 71,384,496 (GRCm39)		noncoding transcript	Het
Gm4953	T	A	1: 158,995,929 (GRCm39)		noncoding transcript	Het
Gorasp2	T	C	2: 70,518,683 (GRCm39)		probably benign	Het
Gse1	T	C	8: 121,295,205 (GRCm39)		probably benign	Het
H2-D1	A	G	17: 35,484,973 (GRCm39)	E278G	probably damaging	Het
Herc2	T	C	7: 55,877,640 (GRCm39)	L4569P	probably damaging	Het
Hgs	T	A	11: 120,368,028 (GRCm39)	S246T	probably damaging	Het
Hs2st1	T	C	3: 144,170,843 (GRCm39)	T110A	probably benign	Het
Hydin	A	G	8: 111,322,070 (GRCm39)	Y4574C	probably benign	Het
Ighv1-23	C	A	12: 114,728,372 (GRCm39)	V17F	possibly damaging	Het
Ighv8-14	A	T	12: 115,772,185 (GRCm39)		noncoding transcript	Het
Igsf21	C	T	4: 139,761,934 (GRCm39)	R248H	probably benign	Het
Il22b	T	C	10: 118,130,894 (GRCm39)	M1V	probably null	Het
Il23r	T	C	6: 67,400,545 (GRCm39)	N595S	probably benign	Het
Il5ra	G	T	6: 106,692,629 (GRCm39)	P372Q	probably damaging	Het
Inppl1	A	G	7: 101,481,516 (GRCm39)	V222A	possibly damaging	Het
Ints10	T	C	8: 69,279,964 (GRCm39)	V697A	probably damaging	Het
Isg15	T	A	4: 156,284,217 (GRCm39)	T104S	probably benign	Het
Kif11	A	G	19: 37,406,385 (GRCm39)	T983A	probably benign	Het
Kif3b	A	T	2: 153,159,212 (GRCm39)	K338*	probably null	Het
Kpna2	T	A	11: 106,881,545 (GRCm39)	I362F	probably damaging	Het
Lama2	G	A	10: 27,014,923 (GRCm39)	T1595M	probably damaging	Het
Lrrc36	A	G	8: 106,153,567 (GRCm39)	T126A	probably benign	Het
Lrrk1	T	A	7: 65,945,202 (GRCm39)	D604V	probably damaging	Het
Map7d1	T	C	4: 126,130,484 (GRCm39)	K409E	probably damaging	Het
Mast2	T	C	4: 116,210,254 (GRCm39)	T110A	probably benign	Het
Micall2	G	A	5: 139,702,580 (GRCm39)	T221M	probably damaging	Het
Morc2b	T	A	17: 33,356,351 (GRCm39)	N474Y	probably damaging	Het
Myo1e	T	A	9: 70,250,378 (GRCm39)	M528K	probably benign	Het
Nlrp1c-ps	G	T	11: 71,151,195 (GRCm39)		noncoding transcript	Het
Nostrin	T	C	2: 68,991,486 (GRCm39)	S160P	possibly damaging	Het
Nup133	T	C	8: 124,653,870 (GRCm39)	R530G	possibly damaging	Het
Or2f2	T	A	6: 42,767,138 (GRCm39)	L55H	probably damaging	Het
Or5p64	T	C	7: 107,855,244 (GRCm39)	I34V	possibly damaging	Het
Or8c16	A	G	9: 38,130,392 (GRCm39)	E91G	probably damaging	Het
Pabpc4l	A	T	3: 46,400,597 (GRCm39)	M349K	possibly damaging	Het
Pah	G	A	10: 87,406,129 (GRCm39)	G256S	probably benign	Het
Pigk	T	C	3: 152,445,841 (GRCm39)	S151P	probably damaging	Het
Plcd4	A	C	1: 74,603,572 (GRCm39)	T658P	possibly damaging	Het
Pld4	A	T	12: 112,730,951 (GRCm39)	S178C	probably benign	Het
Polr1a	G	A	6: 71,886,213 (GRCm39)	E23K	possibly damaging	Het
Pomt1	T	C	2: 32,131,691 (GRCm39)	S127P	probably damaging	Het
Pon2	A	G	6: 5,269,029 (GRCm39)	V215A	possibly damaging	Het
Ppp3cb	A	T	14: 20,559,508 (GRCm39)	M416K	probably damaging	Het
Prdm1	A	T	10: 44,318,048 (GRCm39)	N273K	possibly damaging	Het
Prg4	T	A	1: 150,331,574 (GRCm39)		probably benign	Het
Prkn	G	T	17: 11,059,359 (GRCm39)		probably benign	Het
Ptch1	G	A	13: 63,670,866 (GRCm39)	T888M	probably damaging	Het
Pusl1	T	G	4: 155,975,899 (GRCm39)		probably benign	Het
Rapgef6	A	T	11: 54,513,143 (GRCm39)	E122D	probably damaging	Het
Rif1	C	T	2: 52,000,530 (GRCm39)	T1328I	probably damaging	Het
Rngtt	T	G	4: 33,500,292 (GRCm39)		probably null	Het
Runx2	G	A	17: 45,125,079 (GRCm39)	P80L	probably damaging	Het
Samd1	A	G	8: 84,725,618 (GRCm39)		probably benign	Het
Scamp5	A	T	9: 57,358,735 (GRCm39)	F14I	probably damaging	Het
Shank3	C	T	15: 89,388,547 (GRCm39)	R380C	probably damaging	Het
Shisal2a	T	C	4: 108,234,658 (GRCm39)	T70A	probably benign	Het
Slc1a4	C	A	11: 20,282,166 (GRCm39)	A103S	probably damaging	Het
Slc39a14	A	G	14: 70,547,371 (GRCm39)	V325A	probably benign	Het
Slc5a9	C	A	4: 111,748,941 (GRCm39)		probably null	Het
Spata6l	G	A	19: 28,874,903 (GRCm39)		probably benign	Het
Spta1	T	A	1: 174,013,213 (GRCm39)	S375T	probably damaging	Het
Sval1	A	G	6: 41,932,378 (GRCm39)	N76S	probably benign	Het
Synpo	C	T	18: 60,762,936 (GRCm39)		probably benign	Het
Sytl5	C	T	X: 9,781,841 (GRCm39)	P181L	possibly damaging	Het
Tacc1	A	G	8: 25,672,622 (GRCm39)	M111T	possibly damaging	Het
Tec	T	C	5: 72,913,694 (GRCm39)	D613G	probably benign	Het
Tecpr2	C	T	12: 110,897,921 (GRCm39)	T391I	possibly damaging	Het
Thop1	G	A	10: 80,909,125 (GRCm39)	G52D	probably damaging	Het
Tmco5	T	A	2: 116,722,689 (GRCm39)	V270D	possibly damaging	Het
Ttn	C	T	2: 76,556,973 (GRCm39)	E28265K	possibly damaging	Het
Tuft1	T	C	3: 94,542,750 (GRCm39)	D72G	probably damaging	Het
Tyrp1	C	T	4: 80,769,144 (GRCm39)		probably benign	Het
Usp9y	T	C	Y: 1,308,041 (GRCm39)	D2265G	probably damaging	Het
Vmn2r104	G	A	17: 20,250,288 (GRCm39)	A661V	probably benign	Het
Vwa5a	A	T	9: 38,649,268 (GRCm39)	N672I	probably benign	Het
Wdr95	A	T	5: 149,535,157 (GRCm39)	K772*	probably null	Het
Ybx1	T	C	4: 119,140,010 (GRCm39)	T106A	probably benign	Het
Ypel3	T	C	7: 126,376,961 (GRCm39)	S14P	probably benign	Het
Zfp518a	T	A	19: 40,903,972 (GRCm39)	S1300R	probably benign	Het
Zscan5b	A	T	7: 6,242,189 (GRCm39)	*469Y	probably null	Het

Other mutations in Dhx30

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01343:Dhx30	APN	9	109,915,313 (GRCm39)	missense	probably benign	0.01
IGL01800:Dhx30	APN	9	109,914,581 (GRCm39)	missense	possibly damaging	0.92
IGL02403:Dhx30	APN	9	109,920,587 (GRCm39)	missense	probably damaging	1.00
IGL02869:Dhx30	APN	9	109,926,251 (GRCm39)	missense	probably damaging	1.00
IGL03177:Dhx30	APN	9	109,917,078 (GRCm39)	missense	possibly damaging	0.75
R0092:Dhx30	UTSW	9	109,914,078 (GRCm39)	missense	possibly damaging	0.94
R0601:Dhx30	UTSW	9	109,915,782 (GRCm39)	splice site	probably null
R1667:Dhx30	UTSW	9	109,914,514 (GRCm39)	missense	possibly damaging	0.48
R1667:Dhx30	UTSW	9	109,914,513 (GRCm39)	missense	possibly damaging	0.91
R1670:Dhx30	UTSW	9	109,914,341 (GRCm39)	missense	possibly damaging	0.86
R1728:Dhx30	UTSW	9	109,927,819 (GRCm39)	missense	probably damaging	0.98
R1729:Dhx30	UTSW	9	109,927,819 (GRCm39)	missense	probably damaging	0.98
R1795:Dhx30	UTSW	9	109,937,051 (GRCm39)	splice site	probably null
R1854:Dhx30	UTSW	9	109,917,740 (GRCm39)	missense	probably damaging	1.00
R2191:Dhx30	UTSW	9	109,915,186 (GRCm39)	critical splice donor site	probably null
R2219:Dhx30	UTSW	9	109,916,703 (GRCm39)	missense	probably damaging	1.00
R2220:Dhx30	UTSW	9	109,916,703 (GRCm39)	missense	probably damaging	1.00
R2267:Dhx30	UTSW	9	109,916,102 (GRCm39)	missense	probably damaging	1.00
R2374:Dhx30	UTSW	9	109,920,632 (GRCm39)	missense	probably damaging	0.98
R2568:Dhx30	UTSW	9	109,926,263 (GRCm39)	missense	probably damaging	0.99
R2881:Dhx30	UTSW	9	109,927,913 (GRCm39)	nonsense	probably null
R4022:Dhx30	UTSW	9	109,913,465 (GRCm39)	missense	possibly damaging	0.90
R4052:Dhx30	UTSW	9	109,929,889 (GRCm39)	missense	possibly damaging	0.46
R4695:Dhx30	UTSW	9	109,914,356 (GRCm39)	missense	probably damaging	0.98
R4728:Dhx30	UTSW	9	109,916,718 (GRCm39)	missense	probably damaging	1.00
R4892:Dhx30	UTSW	9	109,914,924 (GRCm39)	splice site	probably null
R4937:Dhx30	UTSW	9	109,915,029 (GRCm39)	missense	probably damaging	1.00
R5135:Dhx30	UTSW	9	109,927,863 (GRCm39)	missense	probably damaging	1.00
R5359:Dhx30	UTSW	9	109,922,203 (GRCm39)	missense	probably damaging	0.99
R5462:Dhx30	UTSW	9	109,930,042 (GRCm39)	missense	probably damaging	0.97
R5504:Dhx30	UTSW	9	109,914,278 (GRCm39)	missense	probably benign	0.08
R5797:Dhx30	UTSW	9	109,927,888 (GRCm39)	missense	probably damaging	0.99
R5860:Dhx30	UTSW	9	109,913,645 (GRCm39)	missense	probably damaging	0.98
R6041:Dhx30	UTSW	9	109,913,666 (GRCm39)	missense	probably benign	0.09
R6132:Dhx30	UTSW	9	109,914,847 (GRCm39)	missense	probably damaging	1.00
R6158:Dhx30	UTSW	9	109,916,098 (GRCm39)	missense	probably damaging	1.00
R6475:Dhx30	UTSW	9	109,914,120 (GRCm39)	missense	possibly damaging	0.91
R6818:Dhx30	UTSW	9	109,917,099 (GRCm39)	missense	probably damaging	1.00
R6984:Dhx30	UTSW	9	109,920,485 (GRCm39)	critical splice donor site	probably null
R7412:Dhx30	UTSW	9	109,921,966 (GRCm39)	missense	probably benign
R7477:Dhx30	UTSW	9	109,916,208 (GRCm39)	missense	probably damaging	1.00
R7808:Dhx30	UTSW	9	109,915,270 (GRCm39)	missense	probably benign	0.00
R7982:Dhx30	UTSW	9	109,914,524 (GRCm39)	missense	probably damaging	1.00
R8343:Dhx30	UTSW	9	109,914,569 (GRCm39)	missense	possibly damaging	0.95
R8376:Dhx30	UTSW	9	109,917,707 (GRCm39)	missense	probably benign	0.15
R8434:Dhx30	UTSW	9	109,929,974 (GRCm39)	missense	probably benign
R8831:Dhx30	UTSW	9	109,917,319 (GRCm39)	missense	probably benign	0.01
R8842:Dhx30	UTSW	9	109,914,296 (GRCm39)	missense	probably benign	0.33
R8971:Dhx30	UTSW	9	109,913,513 (GRCm39)	nonsense	probably null
R9001:Dhx30	UTSW	9	109,916,623 (GRCm39)	missense	probably damaging	0.99
R9117:Dhx30	UTSW	9	109,926,164 (GRCm39)	missense	probably damaging	0.99
R9177:Dhx30	UTSW	9	109,915,750 (GRCm39)	missense	probably damaging	0.97
R9189:Dhx30	UTSW	9	109,914,494 (GRCm39)	nonsense	probably null
R9281:Dhx30	UTSW	9	109,929,983 (GRCm39)	missense	probably benign	0.01
R9289:Dhx30	UTSW	9	109,922,189 (GRCm39)	missense	probably benign	0.06
R9289:Dhx30	UTSW	9	109,920,603 (GRCm39)	missense	possibly damaging	0.66
R9424:Dhx30	UTSW	9	109,916,712 (GRCm39)	missense	probably damaging	1.00
R9576:Dhx30	UTSW	9	109,916,712 (GRCm39)	missense	probably damaging	1.00
R9599:Dhx30	UTSW	9	109,914,518 (GRCm39)	missense	possibly damaging	0.75
R9617:Dhx30	UTSW	9	109,926,186 (GRCm39)	missense	probably damaging	1.00
R9647:Dhx30	UTSW	9	109,922,214 (GRCm39)	missense	probably damaging	1.00
R9711:Dhx30	UTSW	9	109,914,103 (GRCm39)	missense	probably benign	0.03
R9715:Dhx30	UTSW	9	109,916,718 (GRCm39)	missense	probably damaging	1.00
X0027:Dhx30	UTSW	9	109,913,502 (GRCm39)	missense	possibly damaging	0.87
Z1176:Dhx30	UTSW	9	109,916,033 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TAACAGCAAGGCAGAGACTC -3'
(R):5'- AATGCTGCCAGTTGCTGAG -3'

Sequencing Primer
(F):5'- TTTCCAGAATAAAGCCTTGACCGG -3'
(R):5'- TGAGCCCTCGAAGGTACTGTC -3'

Posted On

2016-04-15