Mutagenetix > Incidental Mutation

Incidental Mutation 'R0244:Zfp882'

37976

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

038482-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R0244 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 71913523 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 105 (I105F)

Ref Sequence

ENSEMBL: ENSMUSP00000119978 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably benign
Transcript: ENSMUST00000110002
AA Change: I65F

PolyPhen 2 Score 0.219 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: I65F

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000118290

Predicted Effect

probably benign
Transcript: ENSMUST00000125802
AA Change: I73F

PolyPhen 2 Score 0.156 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857
AA Change: I73F

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000126607
AA Change: I105F

PolyPhen 2 Score 0.793 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857
AA Change: I105F

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170898

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.5%

Validation Efficiency

99% (88/89)

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610507B11Rik	T	A	11: 78,286,491 (GRCm38)		probably null	Het
Adamdec1	A	T	14: 68,568,723 (GRCm38)	C434*	probably null	Het
Adprhl1	A	G	8: 13,242,391 (GRCm38)		probably benign	Het
Ago1	T	A	4: 126,463,706 (GRCm38)	I59F	possibly damaging	Het
Arel1	T	C	12: 84,920,693 (GRCm38)	T786A	probably damaging	Het
Arhgap26	A	G	18: 39,363,131 (GRCm38)	K117R	probably benign	Het
Atp6v0b	C	T	4: 117,884,622 (GRCm38)	G204D	probably damaging	Het
Bace2	T	A	16: 97,436,773 (GRCm38)		probably null	Het
Camk4	G	A	18: 33,179,625 (GRCm38)		probably null	Het
Cdh26	C	T	2: 178,481,632 (GRCm38)	R675C	possibly damaging	Het
Cep152	T	C	2: 125,564,214 (GRCm38)	E1466G	probably benign	Het
Ces3b	T	C	8: 105,092,635 (GRCm38)	F441S	probably damaging	Het
Cfap52	T	C	11: 67,926,382 (GRCm38)	T562A	possibly damaging	Het
Clca3a2	C	A	3: 144,813,898 (GRCm38)	M238I	possibly damaging	Het
Cntnap5c	A	T	17: 58,102,168 (GRCm38)	D467V	probably damaging	Het
Col7a1	T	A	9: 108,972,184 (GRCm38)		probably null	Het
Cstf1	A	G	2: 172,377,710 (GRCm38)	N247S	possibly damaging	Het
Dffb	G	T	4: 153,974,615 (GRCm38)	N68K	probably benign	Het
Duox2	C	T	2: 122,291,860 (GRCm38)	G595S	probably benign	Het
Eftud2	T	A	11: 102,864,725 (GRCm38)	I228F	probably damaging	Het
Elmo3	T	C	8: 105,309,171 (GRCm38)	V578A	probably benign	Het
Elp2	A	G	18: 24,631,471 (GRCm38)	D625G	possibly damaging	Het
Ep300	C	T	15: 81,640,128 (GRCm38)	P1386S	unknown	Het
Fam120b	A	G	17: 15,417,637 (GRCm38)	D610G	probably damaging	Het
Fastk	A	T	5: 24,442,178 (GRCm38)		probably benign	Het
Fbxl6	A	G	15: 76,537,191 (GRCm38)	S252P	probably damaging	Het
Fbxo43	T	C	15: 36,161,793 (GRCm38)	K423E	probably damaging	Het
Filip1	T	A	9: 79,819,462 (GRCm38)	E625V	possibly damaging	Het
Fkbp9	T	A	6: 56,856,378 (GRCm38)	Y283*	probably null	Het
Gigyf2	T	A	1: 87,379,015 (GRCm38)	D142E	possibly damaging	Het
Gm10142	T	C	10: 77,716,014 (GRCm38)		probably null	Het
Golga5	T	C	12: 102,476,188 (GRCm38)	V262A	probably benign	Het
Hectd4	T	C	5: 121,329,605 (GRCm38)	V2539A	probably benign	Het
Ica1	G	T	6: 8,653,632 (GRCm38)	S335*	probably null	Het
Itga1	A	T	13: 115,006,897 (GRCm38)		probably benign	Het
Itgb1	T	C	8: 128,717,685 (GRCm38)		probably benign	Het
Itpr1	G	A	6: 108,473,589 (GRCm38)	V1960I	probably benign	Het
Kcnh4	C	T	11: 100,746,932 (GRCm38)	G633E	probably benign	Het
Kprp	C	T	3: 92,825,411 (GRCm38)	V111I	probably benign	Het
Lamc3	T	C	2: 31,940,721 (GRCm38)	I1490T	probably damaging	Het
Lcp1	A	T	14: 75,227,001 (GRCm38)	D554V	possibly damaging	Het
Lgi3	A	G	14: 70,534,698 (GRCm38)	T228A	probably benign	Het
Lipa	A	T	19: 34,501,541 (GRCm38)	F260I	probably damaging	Het
Lrriq1	C	T	10: 103,215,773 (GRCm38)	E373K	probably damaging	Het
Map6	G	A	7: 99,336,836 (GRCm38)	G649D	probably benign	Het
Mccc1	A	G	3: 35,990,047 (GRCm38)		probably null	Het
Mical3	A	T	6: 120,957,722 (GRCm38)	S1799T	probably benign	Het
Mmp23	T	A	4: 155,652,132 (GRCm38)	T151S	probably damaging	Het
Myo1d	T	A	11: 80,674,708 (GRCm38)	N401I	probably damaging	Het
Myo9b	T	A	8: 71,321,813 (GRCm38)	S323T	probably damaging	Het
Nbn	G	T	4: 15,979,353 (GRCm38)	W446L	probably benign	Het
Nedd1	A	T	10: 92,716,265 (GRCm38)		probably benign	Het
Ngef	C	A	1: 87,487,962 (GRCm38)		probably benign	Het
Nup153	A	T	13: 46,693,936 (GRCm38)	N672K	probably benign	Het
Olfr1308	T	C	2: 111,961,016 (GRCm38)	N19S	probably benign	Het
Olfr149	T	A	9: 39,702,173 (GRCm38)	I199F	probably damaging	Het
Olfr1509	T	C	14: 52,450,512 (GRCm38)	V33A	probably benign	Het
Olfr372	C	T	8: 72,058,400 (GRCm38)	T240M	probably damaging	Het
Palm2	T	G	4: 57,710,177 (GRCm38)	V374G	possibly damaging	Het
Pdlim3	C	A	8: 45,908,460 (GRCm38)		probably benign	Het
Pmfbp1	G	A	8: 109,541,673 (GRCm38)	E951K	probably damaging	Het
Pop1	T	A	15: 34,515,891 (GRCm38)	C548*	probably null	Het
Ppp1r16a	C	T	15: 76,690,799 (GRCm38)		probably benign	Het
Ptpdc1	A	T	13: 48,585,980 (GRCm38)	N658K	probably benign	Het
Ptprk	A	C	10: 28,206,225 (GRCm38)	E63D	possibly damaging	Het
Rtf1	C	T	2: 119,732,877 (GRCm38)	R712W	probably damaging	Het
Samd7	A	C	3: 30,751,073 (GRCm38)	T2P	probably benign	Het
Sft2d1	A	G	17: 8,319,422 (GRCm38)	T52A	probably benign	Het
Slc25a26	A	G	6: 94,510,833 (GRCm38)	H91R	probably damaging	Het
Slc5a4a	A	G	10: 76,189,152 (GRCm38)	E621G	possibly damaging	Het
Slf1	A	T	13: 77,126,632 (GRCm38)	L28*	probably null	Het
Snapc1	C	T	12: 73,975,032 (GRCm38)	R81C	probably damaging	Het
Sorcs2	G	A	5: 36,397,553 (GRCm38)		probably benign	Het
Tacc2	T	C	7: 130,751,825 (GRCm38)		probably benign	Het
Tas2r140	A	G	6: 133,055,327 (GRCm38)	V156A	possibly damaging	Het
Terf2ip	C	A	8: 112,018,164 (GRCm38)	T371K	possibly damaging	Het
Tifa	C	T	3: 127,796,888 (GRCm38)	L103F	probably damaging	Het
Tmco3	A	G	8: 13,292,037 (GRCm38)	N104D	probably damaging	Het
Tmem259	T	A	10: 79,978,963 (GRCm38)	D240V	probably damaging	Het
Trim60	C	T	8: 65,001,048 (GRCm38)	R183H	probably benign	Het
Trps1	T	C	15: 50,664,743 (GRCm38)	N725D	probably damaging	Het
Ttn	C	T	2: 76,814,806 (GRCm38)	V12902M	probably damaging	Het
Ubxn4	G	A	1: 128,262,904 (GRCm38)	E256K	probably benign	Het
Unc79	A	G	12: 103,112,891 (GRCm38)	K1772E	probably damaging	Het
Vwde	C	T	6: 13,193,126 (GRCm38)	V405I	probably benign	Het
Wdr18	T	A	10: 79,966,408 (GRCm38)	D290E	probably damaging	Het
Wdr92	T	C	11: 17,229,851 (GRCm38)	L284P	probably damaging	Het
Wwc2	G	A	8: 47,900,721 (GRCm38)	A126V	probably benign	Het
Zfp942	A	T	17: 21,928,572 (GRCm38)	C359S	probably benign	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71,913,827 (GRCm38)	missense	probably benign
R0270:Zfp882	UTSW	8	71,914,615 (GRCm38)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71,914,337 (GRCm38)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71,914,686 (GRCm38)	nonsense	probably null
R1299:Zfp882	UTSW	8	71,913,473 (GRCm38)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71,913,609 (GRCm38)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71,914,389 (GRCm38)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71,914,654 (GRCm38)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71,914,360 (GRCm38)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71,913,459 (GRCm38)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71,913,155 (GRCm38)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71,914,505 (GRCm38)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71,914,640 (GRCm38)	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	71,914,286 (GRCm38)	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71,914,673 (GRCm38)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71,913,249 (GRCm38)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71,914,229 (GRCm38)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71,913,141 (GRCm38)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71,914,118 (GRCm38)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	71,914,987 (GRCm38)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	71,914,071 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCCTTATAATGAAGTCGCAGTCTCAC -3'
(R):5'- TGCCCCTGAACAAAACTGTGTCTG -3'

Sequencing Primer
(F):5'- tcacccttcctcctgaaaatac -3'
(R):5'- GTGTCTGCTAAAACTGTCTTCAG -3'

Posted On

2013-05-23