Mutagenetix > Incidental Mutation

Incidental Mutation 'R0399:Olfr215'

38049

Institutional Source

Beutler Lab

Gene Symbol

Olfr215

Ensembl Gene

ENSMUSG00000050654

Gene Name

olfactory receptor 215

Synonyms

MOR119-2, GA_x54KRFPKN04-58217732-58216800

MMRRC Submission

038604-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R0399 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

116580084-116584195 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 116582781 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Isoleucine at position 55 (S55I)

Ref Sequence

ENSEMBL: ENSMUSP00000052425 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061723]

AlphaFold

Q8VF82

Predicted Effect

probably benign
Transcript: ENSMUST00000061723
AA Change: S55I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000052425
Gene: ENSMUSG00000050654
AA Change: S55I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	5e-47	PFAM
Pfam:7TM_GPCR_Srsx	33	258	1.8e-5	PFAM
Pfam:7tm_1	39	288	2.9e-15	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.4%

Validation Efficiency

99% (71/72)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310035C23Rik	A	G	1: 105,750,959 (GRCm38)		probably benign	Het
6030452D12Rik	T	C	8: 106,504,542 (GRCm38)	M120T	unknown	Het
Actr1a	A	T	19: 46,385,011 (GRCm38)		probably null	Het
AI314180	T	C	4: 58,827,047 (GRCm38)	T1029A	possibly damaging	Het
Anapc5	A	T	5: 122,791,753 (GRCm38)	V555D	probably damaging	Het
Aox1	A	G	1: 58,068,849 (GRCm38)		probably null	Het
Arhgap30	A	G	1: 171,404,816 (GRCm38)	E343G	probably damaging	Het
Asap2	C	T	12: 21,217,997 (GRCm38)	T291I	possibly damaging	Het
Atp5a1	T	A	18: 77,781,836 (GRCm38)	Y439*	probably null	Het
Auts2	A	T	5: 131,440,524 (GRCm38)	S428T	probably benign	Het
B3gnt7	T	A	1: 86,305,711 (GRCm38)	C109*	probably null	Het
C4b	C	A	17: 34,728,869 (GRCm38)	Q1657H	probably damaging	Het
Cadm2	A	T	16: 66,747,339 (GRCm38)	L268*	probably null	Het
Cep290	G	A	10: 100,554,400 (GRCm38)		probably benign	Het
Cep68	T	G	11: 20,230,571 (GRCm38)	I687L	probably benign	Het
Chd6	T	A	2: 161,052,688 (GRCm38)	D84V	probably damaging	Het
Clpx	A	G	9: 65,322,769 (GRCm38)	T514A	probably benign	Het
Cox18	A	T	5: 90,215,028 (GRCm38)	C324S	probably benign	Het
Cryzl2	T	C	1: 157,462,016 (GRCm38)	Y75H	probably damaging	Het
Cxcr6	C	T	9: 123,810,951 (GRCm38)	A339V	possibly damaging	Het
Dock1	T	C	7: 135,163,442 (GRCm38)	L1721P	probably benign	Het
Dstyk	T	C	1: 132,453,080 (GRCm38)		probably benign	Het
Ehf	A	G	2: 103,266,870 (GRCm38)	Y246H	probably damaging	Het
Epas1	T	C	17: 86,805,193 (GRCm38)	V73A	probably benign	Het
Filip1	A	G	9: 79,818,310 (GRCm38)	I1009T	possibly damaging	Het
Glis3	A	T	19: 28,298,768 (GRCm38)		probably benign	Het
Gm17333	A	T	16: 77,852,790 (GRCm38)		noncoding transcript	Het
Gpc1	G	A	1: 92,857,309 (GRCm38)	R358H	possibly damaging	Het
Gpr155	A	T	2: 73,370,002 (GRCm38)	I387N	possibly damaging	Het
Gria1	A	G	11: 57,186,027 (GRCm38)	D83G	probably damaging	Het
Grid2	A	G	6: 64,666,052 (GRCm38)	I933V	probably benign	Het
Hhatl	C	T	9: 121,788,762 (GRCm38)	A254T	probably benign	Het
Hook2	A	G	8: 84,993,567 (GRCm38)		probably benign	Het
Ift140	T	A	17: 25,050,340 (GRCm38)	S656R	possibly damaging	Het
Il11ra1	A	T	4: 41,766,185 (GRCm38)	T241S	probably benign	Het
Kank1	A	G	19: 25,411,242 (GRCm38)	I760V	probably benign	Het
Kansl1	T	C	11: 104,424,132 (GRCm38)	E360G	possibly damaging	Het
Klf9	A	T	19: 23,142,082 (GRCm38)	S110C	probably damaging	Het
Klhl31	A	G	9: 77,650,653 (GRCm38)	N217S	probably benign	Het
Lct	T	C	1: 128,300,525 (GRCm38)	Y1077C	probably damaging	Het
Lrrc49	A	T	9: 60,610,246 (GRCm38)		probably benign	Het
Lrrn1	T	A	6: 107,569,120 (GRCm38)	H626Q	probably benign	Het
Mmp28	A	T	11: 83,451,732 (GRCm38)	L40Q	probably damaging	Het
Mroh1	C	T	15: 76,452,099 (GRCm38)	A1530V	probably benign	Het
Myo1e	A	G	9: 70,301,793 (GRCm38)		probably benign	Het
Naa25	A	T	5: 121,435,490 (GRCm38)	M761L	probably benign	Het
Ncln	G	A	10: 81,488,297 (GRCm38)	A465V	probably damaging	Het
Nktr	A	G	9: 121,731,484 (GRCm38)	N98S	probably damaging	Het
Olfr1012	T	C	2: 85,759,904 (GRCm38)	I157M	possibly damaging	Het
Olfr651	T	A	7: 104,553,369 (GRCm38)	V150E	probably benign	Het
Olfr76	G	C	19: 12,120,370 (GRCm38)	A114G	possibly damaging	Het
Olfr878	G	T	9: 37,919,553 (GRCm38)	A304S	possibly damaging	Het
Pacsin2	T	C	15: 83,386,782 (GRCm38)	Y222C	probably damaging	Het
Pcdhb15	C	T	18: 37,474,168 (GRCm38)	T151M	possibly damaging	Het
Plcz1	C	A	6: 140,023,230 (GRCm38)	V161L	possibly damaging	Het
Ppp6c	G	A	2: 39,200,124 (GRCm38)		probably benign	Het
Rhbdf2	T	A	11: 116,603,992 (GRCm38)	Y286F	probably benign	Het
Rtn3	A	T	19: 7,457,876 (GRCm38)	D231E	probably damaging	Het
Slc35c2	A	C	2: 165,280,895 (GRCm38)	Y156*	probably null	Het
Spata46	A	G	1: 170,311,537 (GRCm38)	D35G	probably damaging	Het
Tmed3	A	G	9: 89,702,873 (GRCm38)	F110L	possibly damaging	Het
Tmem104	T	G	11: 115,201,308 (GRCm38)		probably benign	Het
Tpbg	T	A	9: 85,844,938 (GRCm38)	V320E	possibly damaging	Het
Trib2	T	C	12: 15,793,663 (GRCm38)	D190G	probably damaging	Het
Tspan2	A	G	3: 102,759,385 (GRCm38)	T26A	probably damaging	Het
Usp17lb	A	C	7: 104,841,151 (GRCm38)	Y190D	possibly damaging	Het
Utp18	C	T	11: 93,880,147 (GRCm38)		probably benign	Het
Utp20	A	T	10: 88,820,979 (GRCm38)	D121E	probably damaging	Het
Vmn1r80	T	A	7: 12,193,317 (GRCm38)	M118K	possibly damaging	Het
Vmn1r84	T	C	7: 12,361,867 (GRCm38)	S300G	probably benign	Het

Other mutations in Olfr215

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01666:Olfr215	APN	6	116,582,335 (GRCm38)	missense	possibly damaging	0.93
IGL02959:Olfr215	APN	6	116,582,544 (GRCm38)	missense	probably damaging	1.00
IGL03053:Olfr215	APN	6	116,582,245 (GRCm38)	missense	possibly damaging	0.50
R0078:Olfr215	UTSW	6	116,582,740 (GRCm38)	missense	probably damaging	0.96
R0277:Olfr215	UTSW	6	116,582,601 (GRCm38)	missense	probably damaging	0.99
R0323:Olfr215	UTSW	6	116,582,601 (GRCm38)	missense	probably damaging	0.99
R0545:Olfr215	UTSW	6	116,582,656 (GRCm38)	missense	probably benign	0.01
R1213:Olfr215	UTSW	6	116,582,866 (GRCm38)	missense	probably benign	0.00
R1775:Olfr215	UTSW	6	116,582,964 (GRCm38)	start gained	probably benign
R1789:Olfr215	UTSW	6	116,582,697 (GRCm38)	missense	probably damaging	1.00
R4724:Olfr215	UTSW	6	116,582,937 (GRCm38)	missense	probably damaging	1.00
R5391:Olfr215	UTSW	6	116,582,847 (GRCm38)	missense	probably damaging	1.00
R5392:Olfr215	UTSW	6	116,582,418 (GRCm38)	missense	probably damaging	1.00
R5686:Olfr215	UTSW	6	116,582,929 (GRCm38)	missense	probably benign	0.00
R6124:Olfr215	UTSW	6	116,582,485 (GRCm38)	missense	probably benign	0.05
R7080:Olfr215	UTSW	6	116,582,353 (GRCm38)	missense	probably damaging	1.00
R7355:Olfr215	UTSW	6	116,582,955 (GRCm38)	start gained	probably benign
R9084:Olfr215	UTSW	6	116,582,271 (GRCm38)	missense	probably benign	0.03
Z1177:Olfr215	UTSW	6	116,582,553 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCAGAGGCTTGCAAATAGCTACAC -3'
(R):5'- ACCTTGAACAGGCTGCTGAATGAC -3'

Sequencing Primer
(F):5'- TGTCCACTGACATCACAGCTATG -3'
(R):5'- TGCACTGTGAGAATACCTCTG -3'

Posted On

2013-05-23