Mutagenetix > Incidental Mutation

Incidental Mutation 'R4933:Vmn2r63'

380720

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

042533-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.114) question?

Stock #

R4933 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42903251-42933789 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 42903978 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 618 (I618N)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably damaging
Transcript: ENSMUST00000163803
AA Change: I618N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: I618N

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.4%
20x: 89.3%

Validation Efficiency

97% (73/75)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	A	G	7: 41,626,802	E643G	probably damaging	Het
4430402I18Rik	G	T	19: 28,941,775	H195N	possibly damaging	Het
Abca2	T	C	2: 25,444,827	V1937A	probably benign	Het
Acot10	A	T	15: 20,666,330	N108K	possibly damaging	Het
Agtpbp1	A	T	13: 59,500,572	M478K	probably benign	Het
Akirin1	G	A	4: 123,736,858	S191F	probably damaging	Het
Aurkb	T	C	11: 69,048,144		probably benign	Het
Cabyr	T	C	18: 12,744,492		probably benign	Het
Ccp110	A	G	7: 118,725,319	E688G	probably damaging	Het
Champ1	T	A	8: 13,879,137	S432T	probably benign	Het
Crybg1	T	A	10: 43,999,213	N633I	probably damaging	Het
Dagla	A	T	19: 10,269,715		probably null	Het
Dkkl1	A	T	7: 45,211,525	L10Q	probably null	Het
Dnah8	G	A	17: 30,748,568	D2585N	probably benign	Het
E430018J23Rik	A	G	7: 127,393,349	Y30H	probably damaging	Het
Fndc7	G	T	3: 108,876,670	Q208K	probably benign	Het
Gins4	A	T	8: 23,234,780	C53S	probably damaging	Het
Gja8	T	A	3: 96,919,035		probably benign	Het
Golph3l	T	A	3: 95,617,423	N328K	probably benign	Het
Haus6	A	C	4: 86,585,287		probably benign	Het
Hdac5	A	G	11: 102,200,563		probably benign	Het
Ide	A	G	19: 37,277,756	Y883H	unknown	Het
Igf2r	A	G	17: 12,691,877		probably null	Het
Kdm3b	T	C	18: 34,810,393	Y723H	probably damaging	Het
Kif21b	G	A	1: 136,151,325		probably null	Het
Lancl1	A	T	1: 67,021,034	N77K	probably benign	Het
Lyst	T	A	13: 13,637,764	N920K	probably damaging	Het
Lyst	G	A	13: 13,759,378	V3554I	probably benign	Het
Map1a	G	A	2: 121,305,905	A2163T	probably damaging	Het
Mapk7	G	T	11: 61,493,908		probably benign	Het
Myo10	C	A	15: 25,781,118	Q154K	probably damaging	Het
Olfr13	C	T	6: 43,174,321	L112F	probably benign	Het
Olfr132	A	G	17: 38,130,550	I214T	probably damaging	Het
Pcdhgb2	G	A	18: 37,692,214	V753M	probably benign	Het
Pnn	T	A	12: 59,070,227	L195Q	probably damaging	Het
Pot1a	A	G	6: 25,771,541	V227A	possibly damaging	Het
Ppp1r21	T	A	17: 88,547,621	D109E	probably benign	Het
Prr15l	G	A	11: 96,934,762	G73S	probably damaging	Het
Rnf148	A	G	6: 23,654,340	F219S	probably benign	Het
Rnpep	C	A	1: 135,267,026		probably benign	Het
Ryr1	T	C	7: 29,104,298	T643A	probably damaging	Het
Ryr2	A	T	13: 11,945,945	C36S	probably damaging	Het
Shc3	G	T	13: 51,442,769	T406N	probably benign	Het
Slit3	G	T	11: 35,688,593	G1199V	probably damaging	Het
Sptbn5	G	A	2: 120,050,120		noncoding transcript	Het
St8sia6	T	C	2: 13,665,442	N236D	probably damaging	Het
Stpg1	A	T	4: 135,506,416	Q3L	probably benign	Het
Sult3a1	T	A	10: 33,866,554	I59N	probably damaging	Het
Svs1	T	C	6: 48,987,492	S145P	probably damaging	Het
Vmn1r208	T	G	13: 22,772,788	I180L	probably benign	Het
Vmn2r51	A	T	7: 10,098,320	N446K	probably damaging	Het
Wrn	T	C	8: 33,322,343	N182S	probably benign	Het
Zfp296	G	T	7: 19,579,712	C164F	possibly damaging	Het
Zmynd8	A	G	2: 165,834,951	V249A	possibly damaging	Het
Zswim2	A	G	2: 83,925,227	L110P	probably damaging	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42904119	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42903364	missense	probably benign
IGL02203:Vmn2r63	APN	7	42904008	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42926850	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42903454	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42929192	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42927944	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42903985	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42927090	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42903275	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42903618	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42933705	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42928528	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42928010	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42928035	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42927915	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42928591	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42904126	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42928319	missense	probably benign
R1698:Vmn2r63	UTSW	7	42933614	missense	probably benign
R1753:Vmn2r63	UTSW	7	42928245	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42926873	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42933580	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42903405	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42928113	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42933826	splice site	probably null
R4649:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42926890	missense	probably benign	0.34
R5198:Vmn2r63	UTSW	7	42903745	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42928277	missense	probably benign
R5400:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42933680	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42929011	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42928635	splice site	probably null
R6362:Vmn2r63	UTSW	7	42903297	missense	probably benign
R6706:Vmn2r63	UTSW	7	42928577	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42903271	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42928535	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42933590	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42926967	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42926982	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42925269	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42927042	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42928129	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42903531	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42927017	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42928495	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42928450	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42926989	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42903937	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42928559	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- CCATTCTAGCTGGAGAGGTG -3'
(R):5'- TGTGTGCAGTGTCCAGAAAGTC -3'

Sequencing Primer
(F):5'- GTGACCTTGAATGCAGTAACC -3'
(R):5'- TGCAGTGTCCAGAAAGTCACTATG -3'

Posted On

2016-04-15