Mutagenetix > Incidental Mutation

Incidental Mutation 'R4928:Fat4'

380972

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

042529-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4928 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38886940-39011985 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 39010465 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Aspartic acid at position 4857 (Y4857D)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061260
AA Change: Y4857D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: Y4857D

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196190

Meta Mutation Damage Score

0.1320

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.5%

Validation Efficiency

98% (123/126)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A530021J07Rik	G	T	7: 83,155,878		noncoding transcript	Het
Aasdh	T	A	5: 76,896,688	K118N	possibly damaging	Het
Abca14	A	T	7: 120,324,580	N1620I	possibly damaging	Het
Acap1	A	T	11: 69,885,815	S149T	possibly damaging	Het
Adgre1	T	A	17: 57,444,064	Y579*	probably null	Het
AI314180	A	G	4: 58,827,073	V1020A	probably damaging	Het
Aifm3	T	C	16: 17,500,432		probably benign	Het
Aldh16a1	A	G	7: 45,141,961	W107R	probably damaging	Het
Amer2	A	T	14: 60,379,445	H363L	possibly damaging	Het
Arhgap10	A	G	8: 77,426,328		probably null	Het
Arhgef6	A	T	X: 57,234,878	D742E	probably damaging	Het
Astn2	A	T	4: 65,729,407	N731K	probably damaging	Het
Atp1a2	T	C	1: 172,278,387	T904A	possibly damaging	Het
Atr	C	T	9: 95,907,299	R1503W	probably damaging	Het
Cdh3	G	T	8: 106,536,610	R97L	probably benign	Het
Chst2	C	T	9: 95,406,006	V96I	probably benign	Het
Cit	T	A	5: 115,985,797	N1464K	probably benign	Het
Col17a1	A	T	19: 47,670,458		probably null	Het
Col4a3	G	A	1: 82,710,977		probably benign	Het
Copz1	T	A	15: 103,291,330	S57R	probably damaging	Het
Cpped1	A	T	16: 11,828,279	F227Y	probably damaging	Het
Cyp2j12	A	T	4: 96,102,151		probably null	Het
Dctn1	T	A	6: 83,189,207	I195N	possibly damaging	Het
Dhrs7b	A	G	11: 60,851,925	I148V	probably benign	Het
Dnah17	T	C	11: 118,027,433	D4096G	probably damaging	Het
Dnajc13	T	C	9: 104,233,638	N145D	possibly damaging	Het
Ercc6l2	T	C	13: 63,894,813		probably benign	Het
Fads6	T	G	11: 115,296,561	I103L	probably benign	Het
Fam189a1	G	T	7: 64,759,368	S426*	probably null	Het
Fbxl14	T	A	6: 119,480,710	L284Q	probably damaging	Het
Fkbp9	T	C	6: 56,849,670	V85A	possibly damaging	Het
Galnt13	T	A	2: 54,516,565	V9E	probably damaging	Het
Gm14412	A	T	2: 177,314,580	S507R	probably benign	Het
Gm15142	T	A	X: 154,638,419		noncoding transcript	Het
Gm4787	T	C	12: 81,378,838	E182G	probably benign	Het
Gm6811	C	A	17: 21,094,631		noncoding transcript	Het
Gm9791	A	T	3: 34,005,069		noncoding transcript	Het
Hoxb7	A	T	11: 96,289,510		probably null	Het
Ift122	T	A	6: 115,915,858		probably benign	Het
Krt28	C	T	11: 99,374,632	V70I	probably benign	Het
Lig1	T	C	7: 13,298,738	S459P	probably damaging	Het
Lrfn3	C	T	7: 30,360,623	R59H	possibly damaging	Het
Mavs	T	C	2: 131,246,743	V489A	probably benign	Het
Mcm8	C	T	2: 132,839,479	P625L	probably benign	Het
Megf10	G	T	18: 57,240,673	R181L	probably benign	Het
Mgrn1	G	A	16: 4,927,862	G440D	probably benign	Het
Mllt3	T	C	4: 87,782,405		probably null	Het
Muc5ac	T	A	7: 141,817,902	Y2613*	probably null	Het
Myh14	C	T	7: 44,635,502	G662S	probably benign	Het
Myod1	T	A	7: 46,377,050	N126K	probably damaging	Het
Nae1	A	T	8: 104,516,142	H439Q	possibly damaging	Het
Narf	T	A	11: 121,244,939	V136E	possibly damaging	Het
Ncapd3	T	A	9: 27,071,735	C926*	probably null	Het
Ndufv2	C	A	17: 66,092,658		probably null	Het
Neb	T	C	2: 52,212,975	S449G	possibly damaging	Het
Nek10	C	T	14: 14,930,577	P698L	probably damaging	Het
Nox3	T	A	17: 3,635,275	E566V	probably null	Het
Oas1a	C	T	5: 120,905,724	R115H	probably benign	Het
Olfr1076	T	A	2: 86,509,125	L222H	probably damaging	Het
Olfr1130	C	A	2: 87,608,143	L252I	probably benign	Het
Olfr1152	A	G	2: 87,868,230	M80V	probably benign	Het
Olfr65	A	G	7: 103,906,672	T78A	probably damaging	Het
Pbld2	A	G	10: 63,047,999	H142R	probably damaging	Het
Pcdha4	C	T	18: 36,954,816	T684M	probably benign	Het
Phc3	G	T	3: 30,950,919	T175N	probably damaging	Het
Pigs	G	T	11: 78,329,002	V68L	probably damaging	Het
Pik3c2g	T	A	6: 139,967,802	D857E	possibly damaging	Het
Pitpnm3	G	A	11: 72,063,172	P550S	probably damaging	Het
Pla2g15	G	A	8: 106,163,218	W374*	probably null	Het
Ptpn14	G	A	1: 189,822,642	C133Y	probably damaging	Het
Ptpn20	A	G	14: 33,614,489	N95S	probably benign	Het
Ptrh2	G	T	11: 86,690,036	V160F	probably damaging	Het
Rapgef3	T	C	15: 97,757,375	D486G	probably damaging	Het
Rev3l	T	G	10: 39,823,985	S1493A	probably benign	Het
Rgs9	T	A	11: 109,225,744	D411V	probably benign	Het
Rgsl1	A	T	1: 153,793,768	Y291N	probably damaging	Het
Rprd2	A	G	3: 95,764,537	Y1185H	probably damaging	Het
Rpusd3	A	G	6: 113,416,206		probably benign	Het
Scnn1a	T	C	6: 125,322,173	I72T	probably damaging	Het
Sdk1	A	T	5: 141,857,003		probably benign	Het
Sfmbt2	T	C	2: 10,445,745	L277P	probably benign	Het
Sgf29	A	G	7: 126,664,982	E73G	probably damaging	Het
Slc12a6	T	A	2: 112,352,961	F764L	probably damaging	Het
Slc4a2	T	C	5: 24,435,342		probably null	Het
Slc9a3	T	G	13: 74,157,719	V285G	probably damaging	Het
Slc9c1	A	G	16: 45,575,409	T608A	probably benign	Het
Slfnl1	C	T	4: 120,535,685	R325C	probably damaging	Het
Smarcad1	T	A	6: 65,074,914	F344I	probably benign	Het
Snrnp70	A	T	7: 45,377,281		probably null	Het
Sod2	C	T	17: 13,008,186	T9M	probably benign	Het
Spag5	T	A	11: 78,314,373	S633T	probably damaging	Het
Spta1	A	T	1: 174,191,056	I531L	probably benign	Het
Stra8	A	G	6: 34,933,156	E60G	probably benign	Het
Sult2a3	T	A	7: 14,111,557	I126F	probably benign	Het
Syt1	T	C	10: 108,504,512	H315R	possibly damaging	Het
Tanc2	T	C	11: 105,867,762	L783P	probably damaging	Het
Thbs2	C	T	17: 14,678,900	C646Y	probably damaging	Het
Ticam2	T	A	18: 46,560,922	K33*	probably null	Het
Trim17	T	C	11: 58,954,301		probably benign	Het
Tsr1	G	A	11: 74,907,879	M691I	probably benign	Het
Ttn	T	A	2: 76,762,419	I20790F	probably damaging	Het
Tubb2b	A	T	13: 34,128,185	Y208*	probably null	Het
Ubr1	T	A	2: 120,914,938	I890F	probably damaging	Het
Usp54	T	C	14: 20,562,192	E852G	probably damaging	Het
Vmn2r78	T	C	7: 86,954,627	V671A	probably damaging	Het
Wipi1	T	C	11: 109,579,649	K315E	probably benign	Het
Xrn2	T	C	2: 147,051,718	V735A	possibly damaging	Het
Zdbf2	T	A	1: 63,308,814	D2117E	possibly damaging	Het
Zfp287	A	T	11: 62,714,136	C648*	probably null	Het
Zfp369	A	G	13: 65,296,800	T586A	possibly damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	38,982,249 (GRCm38)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,889,039 (GRCm38)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	38,981,145 (GRCm38)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,890,673 (GRCm38)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,890,579 (GRCm38)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	38,957,269 (GRCm38)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,007,201 (GRCm38)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	38,889,991 (GRCm38)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,980,658 (GRCm38)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,887,498 (GRCm38)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,891,181 (GRCm38)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,888,070 (GRCm38)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	38,949,534 (GRCm38)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,009,257 (GRCm38)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,887,758 (GRCm38)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,888,947 (GRCm38)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,010,375 (GRCm38)	nonsense	probably null
IGL01626:Fat4	APN	3	38,951,032 (GRCm38)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	38,991,731 (GRCm38)	nonsense	probably null
IGL01800:Fat4	APN	3	38,981,729 (GRCm38)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,888,773 (GRCm38)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	38,970,619 (GRCm38)	splice site	probably benign
IGL01917:Fat4	APN	3	38,889,730 (GRCm38)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	38,979,774 (GRCm38)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,010,271 (GRCm38)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,889,199 (GRCm38)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	38,982,939 (GRCm38)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,888,404 (GRCm38)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	38,996,205 (GRCm38)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,890,546 (GRCm38)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	38,951,263 (GRCm38)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,891,853 (GRCm38)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,001,139 (GRCm38)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	38,979,919 (GRCm38)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,009,988 (GRCm38)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,887,630 (GRCm38)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	38,951,131 (GRCm38)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	38,983,046 (GRCm38)	missense	probably benign
IGL02478:Fat4	APN	3	38,888,215 (GRCm38)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,010,430 (GRCm38)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,887,245 (GRCm38)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,002,764 (GRCm38)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,002,836 (GRCm38)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	38,983,337 (GRCm38)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	38,951,086 (GRCm38)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,890,595 (GRCm38)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,889,295 (GRCm38)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	38,956,744 (GRCm38)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	38,951,273 (GRCm38)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,890,843 (GRCm38)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	38,957,155 (GRCm38)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	38,958,525 (GRCm38)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	38,982,591 (GRCm38)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	38,981,552 (GRCm38)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	38,956,859 (GRCm38)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	38,991,685 (GRCm38)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	38,957,398 (GRCm38)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	38,981,241 (GRCm38)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,009,703 (GRCm38)	missense	probably benign
IGL03371:Fat4	APN	3	38,983,187 (GRCm38)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,889,134 (GRCm38)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,892,019 (GRCm38)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,009,364 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	38,958,450 (GRCm38)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,891,176 (GRCm38)	missense	probably damaging	1.00
Asahi	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
heineken	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
schlitz	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	38,982,357 (GRCm38)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,889,004 (GRCm38)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,888,931 (GRCm38)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,891,596 (GRCm38)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,890,816 (GRCm38)	missense	probably benign
R0357:Fat4	UTSW	3	38,891,227 (GRCm38)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	38,977,413 (GRCm38)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	38,980,637 (GRCm38)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,002,924 (GRCm38)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,888,314 (GRCm38)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	38,981,721 (GRCm38)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,942,870 (GRCm38)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,000,172 (GRCm38)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,889,694 (GRCm38)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,001,178 (GRCm38)	missense	probably benign
R0729:Fat4	UTSW	3	39,000,295 (GRCm38)	splice site	probably benign
R0748:Fat4	UTSW	3	38,887,828 (GRCm38)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	38,999,109 (GRCm38)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	38,995,998 (GRCm38)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	38,982,858 (GRCm38)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,891,392 (GRCm38)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	38,983,086 (GRCm38)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	38,979,825 (GRCm38)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	38,982,942 (GRCm38)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,890,371 (GRCm38)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,890,813 (GRCm38)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,888,323 (GRCm38)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	38,995,917 (GRCm38)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	38,983,076 (GRCm38)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,890,089 (GRCm38)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,888,986 (GRCm38)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,888,860 (GRCm38)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,007,177 (GRCm38)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	38,957,318 (GRCm38)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	38,980,779 (GRCm38)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	38,951,155 (GRCm38)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,891,310 (GRCm38)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,887,489 (GRCm38)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,010,268 (GRCm38)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	38,983,458 (GRCm38)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	38,983,571 (GRCm38)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	38,982,383 (GRCm38)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,010,484 (GRCm38)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	38,981,072 (GRCm38)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	38,991,664 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,996,090 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,887,115 (GRCm38)	missense	probably benign
R2056:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,010,655 (GRCm38)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,889,673 (GRCm38)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	38,981,484 (GRCm38)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	38,980,733 (GRCm38)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,887,539 (GRCm38)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	38,981,417 (GRCm38)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,891,527 (GRCm38)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,010,215 (GRCm38)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,892,049 (GRCm38)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,888,989 (GRCm38)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,890,422 (GRCm38)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	38,995,899 (GRCm38)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	38,980,011 (GRCm38)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	38,957,105 (GRCm38)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	38,980,419 (GRCm38)	missense	probably benign
R2412:Fat4	UTSW	3	38,957,072 (GRCm38)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	38,980,804 (GRCm38)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	38,982,336 (GRCm38)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,007,153 (GRCm38)	missense	probably benign
R3103:Fat4	UTSW	3	38,891,940 (GRCm38)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,890,791 (GRCm38)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	38,981,274 (GRCm38)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	38,982,438 (GRCm38)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	38,982,346 (GRCm38)	missense	probably benign
R4065:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	38,980,020 (GRCm38)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,887,875 (GRCm38)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,942,809 (GRCm38)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,889,171 (GRCm38)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,891,763 (GRCm38)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	38,958,540 (GRCm38)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,891,294 (GRCm38)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	38,951,283 (GRCm38)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,887,342 (GRCm38)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	38,982,957 (GRCm38)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,888,317 (GRCm38)	missense	probably benign
R4871:Fat4	UTSW	3	38,891,605 (GRCm38)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	38,980,632 (GRCm38)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,007,203 (GRCm38)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	38,957,452 (GRCm38)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	38,980,173 (GRCm38)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,888,289 (GRCm38)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	38,980,797 (GRCm38)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	38,980,191 (GRCm38)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	38,956,868 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,010,378 (GRCm38)	missense	probably benign	0.44
R5363:Fat4	UTSW	3	38,888,005 (GRCm38)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,888,864 (GRCm38)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	38,995,946 (GRCm38)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,887,245 (GRCm38)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,891,346 (GRCm38)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,010,370 (GRCm38)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,887,215 (GRCm38)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,010,274 (GRCm38)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,888,934 (GRCm38)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,002,968 (GRCm38)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,889,625 (GRCm38)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	38,949,576 (GRCm38)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	38,983,134 (GRCm38)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,889,468 (GRCm38)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	38,980,955 (GRCm38)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,891,787 (GRCm38)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,007,305 (GRCm38)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,887,336 (GRCm38)	missense	probably benign
R5933:Fat4	UTSW	3	38,951,375 (GRCm38)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	38,951,239 (GRCm38)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	38,983,206 (GRCm38)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,010,547 (GRCm38)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,002,802 (GRCm38)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	38,983,262 (GRCm38)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	38,951,356 (GRCm38)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,007,246 (GRCm38)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,007,080 (GRCm38)	splice site	probably null
R6387:Fat4	UTSW	3	38,983,785 (GRCm38)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	38,983,979 (GRCm38)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,890,887 (GRCm38)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	38,981,269 (GRCm38)	nonsense	probably null
R6503:Fat4	UTSW	3	38,982,257 (GRCm38)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,002,871 (GRCm38)	missense	probably benign
R6566:Fat4	UTSW	3	38,957,126 (GRCm38)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	38,979,690 (GRCm38)	missense	probably benign
R6590:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,942,928 (GRCm38)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	38,956,821 (GRCm38)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	38,982,440 (GRCm38)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	38,983,939 (GRCm38)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	38,957,525 (GRCm38)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	38,981,817 (GRCm38)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	38,996,204 (GRCm38)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,009,446 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,981,775 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,995,971 (GRCm38)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,891,543 (GRCm38)	missense	probably benign
R7030:Fat4	UTSW	3	38,981,958 (GRCm38)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,010,811 (GRCm38)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,010,810 (GRCm38)	missense	probably benign
R7045:Fat4	UTSW	3	38,888,601 (GRCm38)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,889,874 (GRCm38)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,010,533 (GRCm38)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	38,980,787 (GRCm38)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	38,980,464 (GRCm38)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	38,983,895 (GRCm38)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,888,884 (GRCm38)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	38,977,362 (GRCm38)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	38,999,087 (GRCm38)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,891,043 (GRCm38)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	38,980,176 (GRCm38)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,890,413 (GRCm38)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	38,983,840 (GRCm38)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,889,693 (GRCm38)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,889,145 (GRCm38)	nonsense	probably null
R7372:Fat4	UTSW	3	38,890,209 (GRCm38)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,887,924 (GRCm38)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,000,236 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,009,644 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,887,450 (GRCm38)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,009,157 (GRCm38)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	38,957,427 (GRCm38)	nonsense	probably null
R7501:Fat4	UTSW	3	38,958,448 (GRCm38)	nonsense	probably null
R7533:Fat4	UTSW	3	39,007,257 (GRCm38)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	38,981,355 (GRCm38)	missense	possibly damaging	0.56
R7542:Fat4	UTSW	3	38,981,621 (GRCm38)	missense	possibly damaging	0.64
R7548:Fat4	UTSW	3	38,981,114 (GRCm38)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,889,336 (GRCm38)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,010,241 (GRCm38)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	38,981,160 (GRCm38)	missense	probably benign
R7665:Fat4	UTSW	3	38,889,178 (GRCm38)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,891,697 (GRCm38)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,001,204 (GRCm38)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,887,851 (GRCm38)	missense	probably benign
R7883:Fat4	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	38,949,439 (GRCm38)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,887,541 (GRCm38)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	38,981,916 (GRCm38)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	38,981,666 (GRCm38)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,891,203 (GRCm38)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,892,054 (GRCm38)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	38,979,732 (GRCm38)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	38,958,510 (GRCm38)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,008,979 (GRCm38)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	38,979,675 (GRCm38)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	38,981,553 (GRCm38)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	38,981,903 (GRCm38)	missense	probably benign
R8543:Fat4	UTSW	3	38,977,494 (GRCm38)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,010,693 (GRCm38)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,010,498 (GRCm38)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,888,443 (GRCm38)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,891,853 (GRCm38)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	38,979,749 (GRCm38)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	38,999,129 (GRCm38)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,892,120 (GRCm38)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	38,983,629 (GRCm38)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,887,175 (GRCm38)	nonsense	probably null
R9071:Fat4	UTSW	3	38,983,449 (GRCm38)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,007,299 (GRCm38)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,010,654 (GRCm38)	missense
R9180:Fat4	UTSW	3	38,888,407 (GRCm38)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	38,982,443 (GRCm38)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,890,930 (GRCm38)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,009,241 (GRCm38)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,888,247 (GRCm38)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,891,022 (GRCm38)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,891,632 (GRCm38)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	38,981,898 (GRCm38)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,891,268 (GRCm38)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,891,263 (GRCm38)	missense
R9456:Fat4	UTSW	3	38,888,422 (GRCm38)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	38,980,653 (GRCm38)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign
R9568:Fat4	UTSW	3	38,892,007 (GRCm38)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	38,981,664 (GRCm38)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,889,183 (GRCm38)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,001,225 (GRCm38)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,009,106 (GRCm38)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	38,981,040 (GRCm38)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,000,151 (GRCm38)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,942,902 (GRCm38)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	38,970,752 (GRCm38)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	38,958,492 (GRCm38)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,887,050 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	38,983,359 (GRCm38)	missense	probably benign	0.00
Z1176:Fat4	UTSW	3	38,983,815 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,890,347 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,981,838 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,888,584 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTAGAGAGGCTCAACACC -3'
(R):5'- CCAGTTCAAAAGGTTGTCCCAG -3'

Sequencing Primer
(F):5'- GAGGCTCAACACCCCTCG -3'
(R):5'- GTTCAAAAGGTTGTCCCAGTTGAAG -3'

Posted On

2016-04-15