Mutagenetix > Incidental Mutations

Incidental Mutation 'R4945:Pbrm1'

381329

Institutional Source

Beutler Lab

Gene Symbol

Pbrm1

Ensembl Gene

ENSMUSG00000042323

Gene Name

polybromo 1

Synonyms

BAF180, Pb1, 2610016F04Rik, 2310032M22Rik

MMRRC Submission

042542-MU

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R4945 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

31019138-31121592 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 31052216 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 446 (D446E)

Ref Sequence

ENSEMBL: ENSMUSP00000087682 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022471] [ENSMUST00000022474] [ENSMUST00000052239] [ENSMUST00000090214] [ENSMUST00000112092] [ENSMUST00000112094] [ENSMUST00000112095] [ENSMUST00000112098] [ENSMUST00000123678] [ENSMUST00000135704] [ENSMUST00000136237] [ENSMUST00000146325] [ENSMUST00000156628]

Predicted Effect

probably benign
Transcript: ENSMUST00000022471
AA Change: D446E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000022471
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	881	2.53e-18	SMART
coiled coil region	907	934	N/A	INTRINSIC
BAH	956	1074	1.33e-45	SMART
low complexity region	1083	1097	N/A	INTRINSIC
BAH	1156	1272	3.02e-35	SMART
low complexity region	1318	1338	N/A	INTRINSIC
HMG	1378	1450	8.91e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000022474
AA Change: D446E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000022474
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	531	642	6.07e-39	SMART
BROMO	666	780	3.01e-43	SMART
BROMO	790	896	2.53e-18	SMART
coiled coil region	922	949	N/A	INTRINSIC
BAH	971	1089	1.33e-45	SMART
low complexity region	1098	1112	N/A	INTRINSIC
BAH	1171	1287	3.02e-35	SMART
low complexity region	1333	1353	N/A	INTRINSIC
HMG	1393	1465	8.91e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000052239
AA Change: D446E

PolyPhen 2 Score 0.118 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000060476
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	881	2.53e-18	SMART
coiled coil region	907	934	N/A	INTRINSIC
BAH	956	1049	8.64e-22	SMART
low complexity region	1058	1072	N/A	INTRINSIC
BAH	1131	1247	3.02e-35	SMART
low complexity region	1293	1310	N/A	INTRINSIC
HMG	1326	1396	2.87e-13	SMART
low complexity region	1405	1430	N/A	INTRINSIC
low complexity region	1449	1477	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000090214
AA Change: D446E

PolyPhen 2 Score 0.968 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000087682
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	881	2.53e-18	SMART
coiled coil region	907	934	N/A	INTRINSIC
BAH	956	1074	1.33e-45	SMART
low complexity region	1083	1097	N/A	INTRINSIC
BAH	1156	1272	3.02e-35	SMART
low complexity region	1318	1338	N/A	INTRINSIC
HMG	1378	1448	1.62e-21	SMART
low complexity region	1464	1475	N/A	INTRINSIC
low complexity region	1485	1500	N/A	INTRINSIC
low complexity region	1512	1537	N/A	INTRINSIC
low complexity region	1556	1584	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112092
AA Change: D446E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000107721
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	531	642	6.07e-39	SMART
BROMO	666	780	3.01e-43	SMART
BROMO	790	896	2.53e-18	SMART
coiled coil region	922	949	N/A	INTRINSIC
BAH	971	1089	1.33e-45	SMART
low complexity region	1098	1112	N/A	INTRINSIC
BAH	1171	1287	3.02e-35	SMART
low complexity region	1333	1353	N/A	INTRINSIC
HMG	1393	1463	1.62e-21	SMART
low complexity region	1479	1490	N/A	INTRINSIC
low complexity region	1519	1547	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112094
AA Change: D414E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000107723
Gene: ENSMUSG00000042323
AA Change: D414E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	322	338	N/A	INTRINSIC
BROMO	347	457	1.57e-32	SMART
BROMO	484	595	6.07e-39	SMART
BROMO	619	733	3.01e-43	SMART
BROMO	743	849	2.53e-18	SMART
coiled coil region	875	902	N/A	INTRINSIC
BAH	924	1042	1.33e-45	SMART
low complexity region	1051	1065	N/A	INTRINSIC
BAH	1124	1240	3.02e-35	SMART
low complexity region	1286	1306	N/A	INTRINSIC
HMG	1346	1416	2.87e-13	SMART
low complexity region	1425	1450	N/A	INTRINSIC
low complexity region	1469	1497	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112095
AA Change: D446E

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000107724
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	881	2.53e-18	SMART
coiled coil region	907	934	N/A	INTRINSIC
BAH	956	1074	1.33e-45	SMART
low complexity region	1083	1097	N/A	INTRINSIC
BAH	1156	1272	3.02e-35	SMART
low complexity region	1318	1338	N/A	INTRINSIC
HMG	1378	1448	2.87e-13	SMART
low complexity region	1457	1482	N/A	INTRINSIC
low complexity region	1501	1529	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112098
AA Change: D446E

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000107727
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	531	642	6.07e-39	SMART
BROMO	666	780	3.01e-43	SMART
BROMO	790	896	2.53e-18	SMART
coiled coil region	922	949	N/A	INTRINSIC
BAH	971	1089	1.33e-45	SMART
low complexity region	1098	1112	N/A	INTRINSIC
BAH	1171	1287	3.02e-35	SMART
low complexity region	1333	1353	N/A	INTRINSIC
HMG	1393	1463	1.62e-21	SMART
low complexity region	1479	1490	N/A	INTRINSIC
low complexity region	1500	1515	N/A	INTRINSIC
low complexity region	1527	1552	N/A	INTRINSIC
low complexity region	1571	1599	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000123678
AA Change: D446E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000120549
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	856	2.3e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135704
AA Change: D390E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000115505
Gene: ENSMUSG00000042323
AA Change: D390E

Domain	Start	End	E-Value	Type
BROMO	1	97	3.24e-25	SMART
BROMO	119	233	5.84e-41	SMART
low complexity region	298	314	N/A	INTRINSIC
BROMO	323	433	1.57e-32	SMART
BROMO	475	586	6.07e-39	SMART
BROMO	610	724	3.01e-43	SMART
BROMO	734	840	2.53e-18	SMART
low complexity region	862	892	N/A	INTRINSIC
BAH	914	1032	1.33e-45	SMART
low complexity region	1041	1055	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000136237
AA Change: D446E

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000119722
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	859	7.08e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000146325
AA Change: D435E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000122805
Gene: ENSMUSG00000042323
AA Change: D435E

Domain	Start	End	E-Value	Type
BROMO	64	174	4.97e-35	SMART
BROMO	196	310	5.84e-41	SMART
low complexity region	343	359	N/A	INTRINSIC
BROMO	368	478	1.57e-32	SMART
BROMO	505	616	6.07e-39	SMART
BROMO	640	754	3.01e-43	SMART
BROMO	764	870	2.53e-18	SMART
coiled coil region	896	923	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000156628
AA Change: D446E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000123546
Gene: ENSMUSG00000042323
AA Change: D446E

Domain	Start	End	E-Value	Type
BROMO	43	153	4.97e-35	SMART
BROMO	175	289	5.84e-41	SMART
low complexity region	354	370	N/A	INTRINSIC
BROMO	379	489	1.57e-32	SMART
BROMO	516	627	6.07e-39	SMART
BROMO	651	765	3.01e-43	SMART
BROMO	775	881	2.53e-18	SMART
low complexity region	903	933	N/A	INTRINSIC
BAH	955	1073	1.33e-45	SMART
low complexity region	1082	1096	N/A	INTRINSIC
BAH	1155	1271	3.02e-35	SMART
low complexity region	1317	1337	N/A	INTRINSIC
HMG	1377	1447	2.87e-13	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012]
PHENOTYPE: Homozygous null mice display embryonic lethality with hypoplastic cardiac ventricular chambers and malformation of the placenta. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430403G16Rik	A	T	5: 109,677,075	C170S	possibly damaging	Het
Adamts13	G	A	2: 26,986,610	R512H	probably damaging	Het
Adgrl2	T	A	3: 148,823,036	M1158L	probably damaging	Het
Adgrl3	C	A	5: 81,512,048	H153Q	probably damaging	Het
Aldh1a2	C	A	9: 71,215,916	Q24K	probably benign	Het
Atp1b1	A	G	1: 164,438,298	V298A	probably damaging	Het
Casp8ap2	T	A	4: 32,631,163	N74K	possibly damaging	Het
Cep135	A	G	5: 76,597,428	D229G	probably benign	Het
Cerk	C	G	15: 86,156,601	D186H	probably benign	Het
Cmtr2	T	C	8: 110,221,433	F125S	probably damaging	Het
Col6a1	A	G	10: 76,712,272	V650A	unknown	Het
Cuzd1	A	G	7: 131,316,621	F196L	probably damaging	Het
Esm1	T	G	13: 113,210,145		probably null	Het
Fbn2	T	A	18: 58,050,253	I1787F	possibly damaging	Het
Gm1110	T	C	9: 26,920,595	M87V	possibly damaging	Het
Gpr179	T	C	11: 97,349,718	H293R	probably damaging	Het
Grk2	A	G	19: 4,290,447	I302T	probably damaging	Het
Hoxb6	A	G	11: 96,299,259	Y28C	possibly damaging	Het
Ift27	A	G	15: 78,164,254	V110A	probably damaging	Het
Igfbp7	T	C	5: 77,351,257	D267G	probably benign	Het
Ighv1-49	C	A	12: 115,055,408	V56L	possibly damaging	Het
Ighv8-8	C	T	12: 115,294,261	M53I	probably benign	Het
Kcnj3	T	A	2: 55,437,578	N126K	probably damaging	Het
Kif12	C	T	4: 63,168,493		probably null	Het
Lrif1	A	C	3: 106,735,753	I725L	probably damaging	Het
Lrrk2	T	C	15: 91,804,920	V2265A	probably benign	Het
Med25	T	C	7: 44,883,102	N438S	possibly damaging	Het
Mex3b	A	T	7: 82,870,174	T566S	probably benign	Het
Mkx	T	C	18: 7,000,657	N95S	possibly damaging	Het
Morc3	A	T	16: 93,871,194	D820V	probably damaging	Het
Mroh4	G	T	15: 74,612,008	N562K	probably benign	Het
Ndst3	A	T	3: 123,552,552	I276N	probably damaging	Het
Notch2	A	G	3: 98,111,721	N733S	probably benign	Het
Olfr1025-ps1	T	A	2: 85,918,573	I216N	possibly damaging	Het
Olfr1084	T	C	2: 86,639,489	Y73C	probably damaging	Het
Olfr168	C	A	16: 19,530,557	R121L	probably benign	Het
Olfr639	G	A	7: 104,012,378	T108I	possibly damaging	Het
Olfr713	A	T	7: 107,036,319	M55L	probably benign	Het
Olfr885	G	T	9: 38,061,772	G151C	probably damaging	Het
Olfr907	A	T	9: 38,499,289	I207F	probably damaging	Het
Olfr994	T	C	2: 85,430,551	T93A	probably benign	Het
Patj	A	T	4: 98,495,064	N268Y	probably damaging	Het
Pcdhga11	A	G	18: 37,758,032	T698A	probably benign	Het
Pde6a	T	C	18: 61,234,718	V266A	probably damaging	Het
Phax	G	T	18: 56,575,991	R209L	probably damaging	Het
Pld2	T	A	11: 70,555,698	M20K	probably damaging	Het
Rabl3	T	C	16: 37,541,858	S20P	probably damaging	Het
Rad51	T	A	2: 119,127,148	V174E	probably damaging	Het
Rb1cc1	A	G	1: 6,249,627	E1090G	probably benign	Het
Rfc3	T	C	5: 151,642,985	E333G	probably damaging	Het
Rfx6	T	G	10: 51,726,851	Y821*	probably null	Het
Riok3	A	G	18: 12,128,915	D2G	probably damaging	Het
Rtn1	G	T	12: 72,217,484	A183E	probably damaging	Het
Skint8	T	C	4: 111,939,608	L303P	probably damaging	Het
Slc36a2	C	A	11: 55,174,694	V162L	probably benign	Het
Sorcs3	T	C	19: 48,764,148	V794A	possibly damaging	Het
Sspo	A	G	6: 48,467,087		probably null	Het
Tbk1	T	C	10: 121,551,269	N707S	probably damaging	Het
Tchhl1	A	T	3: 93,471,576	H529L	probably benign	Het
Tgfbr2	A	T	9: 116,131,565	H106Q	probably benign	Het
Trim8	A	G	19: 46,502,775	E111G	probably benign	Het
Tshz2	A	G	2: 169,883,874	Y130C	probably damaging	Het
Txndc15	T	A	13: 55,718,165	D147E	probably benign	Het
Vmn1r172	T	A	7: 23,660,320	L210H	possibly damaging	Het
Vmn1r55	A	G	7: 5,147,106	V106A	probably damaging	Het
Vmn2r49	T	A	7: 9,986,287	K426*	probably null	Het
Wnk2	C	T	13: 49,057,246	R206Q	probably damaging	Het
Zfp473	T	C	7: 44,734,564	N115S	probably benign	Het
Zfp536	G	T	7: 37,569,736	A85D	probably damaging	Het
Zfp853	C	A	5: 143,288,829	Q345H	unknown	Het

Other mutations in Pbrm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00598:Pbrm1	APN	14	31030927	missense	probably damaging	1.00
IGL00648:Pbrm1	APN	14	31052283	missense	probably damaging	1.00
IGL00654:Pbrm1	APN	14	31032404	splice site	probably benign
IGL00674:Pbrm1	APN	14	31118776	missense	probably damaging	1.00
IGL00701:Pbrm1	APN	14	31052248	missense	probably damaging	1.00
IGL00850:Pbrm1	APN	14	31087619	missense	probably damaging	1.00
IGL01024:Pbrm1	APN	14	31052260	missense	probably damaging	1.00
IGL01924:Pbrm1	APN	14	31082604	missense	probably damaging	1.00
IGL02122:Pbrm1	APN	14	31089616	missense	probably damaging	0.97
IGL02390:Pbrm1	APN	14	31032510	missense	probably benign	0.00
IGL02675:Pbrm1	APN	14	31106287	missense	possibly damaging	0.85
IGL02936:Pbrm1	APN	14	31061513	missense	probably damaging	1.00
IGL02938:Pbrm1	APN	14	31067804	missense	probably damaging	1.00
IGL02997:Pbrm1	APN	14	31061551	missense	probably damaging	1.00
IGL03246:Pbrm1	APN	14	31110549	missense	probably benign	0.00
IGL03290:Pbrm1	APN	14	31107284	missense	probably damaging	1.00
IGL03372:Pbrm1	APN	14	31085019	missense	probably benign	0.04
IGL03386:Pbrm1	APN	14	31050092	missense	probably damaging	1.00
PIT4520001:Pbrm1	UTSW	14	31067861	missense	probably damaging	0.99
R0502:Pbrm1	UTSW	14	31064820	missense	probably benign	0.36
R0552:Pbrm1	UTSW	14	31035959	missense	probably damaging	1.00
R0558:Pbrm1	UTSW	14	31085059	splice site	probably null
R0561:Pbrm1	UTSW	14	31035991	missense	probably benign
R0591:Pbrm1	UTSW	14	31046430	splice site	probably benign
R0835:Pbrm1	UTSW	14	31067579	missense	probably damaging	1.00
R1167:Pbrm1	UTSW	14	31050142	missense	probably damaging	1.00
R1209:Pbrm1	UTSW	14	31118852	missense	probably damaging	1.00
R1259:Pbrm1	UTSW	14	31074814	missense	probably damaging	1.00
R1321:Pbrm1	UTSW	14	31067502	missense	probably damaging	1.00
R1622:Pbrm1	UTSW	14	31032548	missense	probably benign	0.07
R1843:Pbrm1	UTSW	14	31038957	missense	probably damaging	1.00
R1870:Pbrm1	UTSW	14	31106175	missense	probably damaging	0.99
R2202:Pbrm1	UTSW	14	31032449	missense	possibly damaging	0.76
R2203:Pbrm1	UTSW	14	31032449	missense	possibly damaging	0.76
R2247:Pbrm1	UTSW	14	31074893	missense	probably damaging	1.00
R3237:Pbrm1	UTSW	14	31032475	missense	probably damaging	1.00
R4091:Pbrm1	UTSW	14	31036003	missense	probably benign	0.00
R4280:Pbrm1	UTSW	14	31107312	critical splice donor site	probably null
R4379:Pbrm1	UTSW	14	31067706	missense	probably damaging	1.00
R4381:Pbrm1	UTSW	14	31025556	missense	probably benign	0.02
R4816:Pbrm1	UTSW	14	31110448	missense	probably benign	0.32
R4939:Pbrm1	UTSW	14	31061623	missense	probably damaging	1.00
R4958:Pbrm1	UTSW	14	31074827	missense	probably damaging	1.00
R5095:Pbrm1	UTSW	14	31032530	missense	probably benign	0.00
R5276:Pbrm1	UTSW	14	31106184	missense	probably damaging	0.99
R5387:Pbrm1	UTSW	14	31082610	missense	probably damaging	1.00
R5434:Pbrm1	UTSW	14	31085011	missense	probably damaging	0.96
R5476:Pbrm1	UTSW	14	31032519	missense	probably benign	0.00
R5522:Pbrm1	UTSW	14	31089563	missense	probably damaging	1.00
R5548:Pbrm1	UTSW	14	31105424	missense	probably damaging	1.00
R5605:Pbrm1	UTSW	14	31035992	missense	probably benign	0.20
R6089:Pbrm1	UTSW	14	31087585	missense	probably damaging	0.99
R6159:Pbrm1	UTSW	14	31052283	missense	possibly damaging	0.95
R6224:Pbrm1	UTSW	14	31050111	missense	probably benign	0.19
R6335:Pbrm1	UTSW	14	31084095	missense	probably damaging	1.00
R6499:Pbrm1	UTSW	14	31061509	missense	probably damaging	0.99
R6823:Pbrm1	UTSW	14	31084790	missense	probably damaging	1.00
R7002:Pbrm1	UTSW	14	31064820	missense	probably benign	0.36
R7021:Pbrm1	UTSW	14	31067816	missense	probably damaging	0.98
R7216:Pbrm1	UTSW	14	31045422	missense	possibly damaging	0.93
R7588:Pbrm1	UTSW	14	31084943	missense	probably damaging	0.99
R7828:Pbrm1	UTSW	14	31030891	missense	probably damaging	1.00
R8035:Pbrm1	UTSW	14	31084152	missense	probably damaging	1.00
R8188:Pbrm1	UTSW	14	31067816	missense	probably damaging	0.98
R8417:Pbrm1	UTSW	14	31027462	missense	possibly damaging	0.92
R8517:Pbrm1	UTSW	14	31067782	missense	probably benign	0.00
Z1088:Pbrm1	UTSW	14	31110454	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- GGAAGCCTATTACATGGGGC -3'
(R):5'- TCCTCTACAGGGTTCTGACAAGTC -3'

Sequencing Primer
(F):5'- GCCTATTACATGGGGCTAAAAATAC -3'
(R):5'- CTTAATACCCCTGGGATAGAGACGTG -3'

Posted On

2016-04-27