Incidental Mutation 'R4955:Olfr224'
ID381536
Institutional Source Beutler Lab
Gene Symbol Olfr224
Ensembl Gene ENSMUSG00000059279
Gene Nameolfactory receptor 224
SynonymsGA_x6K02T2NKPP-858022-858862, Olfr327-ps1, MOR275-10_p, GA_x6K02T00261-652-347, MOR275-3
MMRRC Submission 042552-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.055) question?
Stock #R4955 (G1)
Quality Score225
Status Validated
Chromosome11
Chromosomal Location58565856-58570553 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 58566518 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Histidine at position 276 (Y276H)
Ref Sequence ENSEMBL: ENSMUSP00000104450 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000081102] [ENSMUST00000108822] [ENSMUST00000215322] [ENSMUST00000216758] [ENSMUST00000217009]
Predicted Effect probably benign
Transcript: ENSMUST00000081102
AA Change: Y276H

PolyPhen 2 Score 0.394 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000079881
Gene: ENSMUSG00000059279
AA Change: Y276H

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srsx 33 299 5.1e-7 PFAM
Pfam:7tm_1 39 288 4.4e-35 PFAM
Pfam:7tm_4 137 281 5.8e-43 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000108822
AA Change: Y276H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000104450
Gene: ENSMUSG00000064252
AA Change: Y276H

DomainStartEndE-ValueType
Pfam:7tm_4 29 306 1.1e-47 PFAM
Pfam:7TM_GPCR_Srsx 33 299 5.1e-7 PFAM
Pfam:7tm_1 39 288 4.6e-28 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000215322
AA Change: Y276H

PolyPhen 2 Score 0.394 (Sensitivity: 0.90; Specificity: 0.89)
Predicted Effect probably benign
Transcript: ENSMUST00000216758
AA Change: Y276H

PolyPhen 2 Score 0.394 (Sensitivity: 0.90; Specificity: 0.89)
Predicted Effect probably benign
Transcript: ENSMUST00000217009
AA Change: Y276H

PolyPhen 2 Score 0.394 (Sensitivity: 0.90; Specificity: 0.89)
Meta Mutation Damage Score 0.3289 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 93.9%
Validation Efficiency 100% (50/50)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3110009E18Rik G C 1: 120,169,110 probably benign Het
3110009E18Rik G T 1: 120,169,119 probably benign Het
3110009E18Rik C T 1: 120,169,120 probably benign Het
4933407L21Rik T A 1: 85,931,287 probably benign Het
Abca8a T G 11: 110,036,512 E1338D probably benign Het
Arl2bp G A 8: 94,670,428 probably null Het
Arsj T C 3: 126,438,540 Y312H probably benign Het
Atp8b2 G T 3: 89,952,920 probably benign Het
Cdh20 T C 1: 104,984,803 V594A probably damaging Het
Cfap44 T A 16: 44,475,277 V1646E possibly damaging Het
Csmd3 A T 15: 48,673,518 I96K probably damaging Het
Dusp27 T C 1: 166,108,092 Y179C probably damaging Het
Fbn2 T C 18: 58,058,383 Q1556R possibly damaging Het
Fstl5 T A 3: 76,223,876 probably null Het
Hist1h4i T A 13: 22,041,185 I47F probably damaging Het
Hivep2 T A 10: 14,130,958 M1100K probably benign Het
Ing4 C T 6: 125,048,201 A225V probably damaging Het
Kcnc3 CTT CT 7: 44,591,296 probably null Het
Khdrbs2 T A 1: 32,520,077 probably benign Het
Kif21a A T 15: 90,937,190 W1566R probably damaging Het
Lrfn5 A C 12: 61,839,978 D184A probably benign Het
Mettl2 C T 11: 105,137,779 T319I possibly damaging Het
Mgrn1 T C 16: 4,934,219 V529A probably benign Het
Naca T A 10: 128,042,215 probably benign Het
Ninj2 A G 6: 120,197,946 N26S probably damaging Het
Nqo1 A G 8: 107,388,857 S263P probably benign Het
Obscn T C 11: 59,069,172 T3566A probably benign Het
Olfr1287 A T 2: 111,449,605 H155L probably damaging Het
Olfr243 A T 7: 103,716,705 Y37F probably benign Het
Olfr761 T C 17: 37,952,898 N42S probably damaging Het
Opn5 A T 17: 42,611,238 F24L probably damaging Het
Palmd A G 3: 116,924,224 V208A probably damaging Het
Plekhd1 T A 12: 80,722,021 I426N possibly damaging Het
Polq T G 16: 37,061,082 Y1203D probably benign Het
Prex1 A G 2: 166,573,223 F251S probably damaging Het
Prkd3 T C 17: 78,952,727 M816V probably null Het
Rab3gap2 C T 1: 185,267,155 probably benign Het
Rcan2 C A 17: 44,037,081 P13Q probably damaging Het
Slc35b3 A G 13: 38,932,890 V329A probably benign Het
Slc5a1 T C 5: 33,160,902 M633T probably benign Het
Stac2 A C 11: 98,043,548 L110R possibly damaging Het
Tecpr1 T C 5: 144,217,257 E126G probably damaging Het
Ttll6 A G 11: 96,138,789 D176G possibly damaging Het
Utrn C T 10: 12,861,567 probably null Het
Zfp341 T C 2: 154,638,030 V467A probably damaging Het
Other mutations in Olfr224
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00321:Olfr224 APN 11 58566767 missense probably damaging 1.00
IGL02327:Olfr224 APN 11 58566935 missense probably damaging 1.00
IGL02904:Olfr224 APN 11 58566797 missense possibly damaging 0.61
IGL02968:Olfr224 APN 11 58567195 missense possibly damaging 0.90
R1704:Olfr224 UTSW 11 58566754 missense probably damaging 0.97
R2055:Olfr224 UTSW 11 58566847 missense probably damaging 0.99
R3925:Olfr224 UTSW 11 58566826 missense probably benign 0.34
R4093:Olfr224 UTSW 11 58566829 missense probably damaging 1.00
R4902:Olfr224 UTSW 11 58566625 missense possibly damaging 0.60
R4956:Olfr224 UTSW 11 58566518 missense probably damaging 1.00
R4957:Olfr224 UTSW 11 58566518 missense probably damaging 1.00
R5436:Olfr224 UTSW 11 58566937 missense probably benign 0.34
R6699:Olfr224 UTSW 11 58567205 missense possibly damaging 0.69
R6825:Olfr224 UTSW 11 58566650 missense possibly damaging 0.89
R7467:Olfr224 UTSW 11 58566462 missense possibly damaging 0.82
R7590:Olfr224 UTSW 11 58567259 missense probably benign 0.09
R7600:Olfr224 UTSW 11 58567336 missense probably benign 0.17
R7725:Olfr224 UTSW 11 58566767 missense probably damaging 1.00
X0026:Olfr224 UTSW 11 58567174 missense possibly damaging 0.50
Predicted Primers PCR Primer
(F):5'- ATCAGCATGAGACCTGGAAC -3'
(R):5'- CTGTGACAGTCATTTCAGGCTC -3'

Sequencing Primer
(F):5'- ATTTCTGAGTTCGAGGCCAGCC -3'
(R):5'- GTGACAGTCATTTCAGGCTCCTATTC -3'
Posted On2016-04-27