|Institutional Source||Beutler Lab|
|Gene Name||free fatty acid receptor 4|
|Synonyms||Gpr129, O3far1, Pgr4, Gpr120|
|Is this an essential gene?||Probably non essential (E-score: 0.114)|
|Stock #||R4956 (G1)|
|Chromosomal Location||38097079-38114263 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 38097580 bp|
|Amino Acid Change||Arginine to Tryptophan at position 152 (R152W)|
|Ref Sequence||ENSEMBL: ENSMUSP00000063660 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000067098]|
|Predicted Effect||probably benign
AA Change: R152W
PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
AA Change: R152W
|Meta Mutation Damage Score||0.1644|
|Coding Region Coverage||
|Validation Efficiency||96% (70/73)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
PHENOTYPE: Homozygotes for a null allele show altered taste responses to fatty acids. Homozygotes for another null allele develop obesity, liver steatosis, and impaired glucose metabolism, adipogenesis and lipogenesis on a high-fat diet. Homozygotes for a third allele show altered islet somatostatin secretion. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ffar4||
(F):5'- ACCTCTTCTGCGCGGATTTG -3'
(R):5'- CGGCTTTGGTCAGATCCTTG -3'
(F):5'- GGATTTGCTCTTCACCAGCG -3'
(R):5'- TTGCCTCCCACCAGCAG -3'