Mutagenetix > Incidental Mutation

Incidental Mutation 'R4957:Cdc25b'

381629

Institutional Source

Beutler Lab

Gene Symbol

Cdc25b

Ensembl Gene

ENSMUSG00000027330

Gene Name

cell division cycle 25B

Synonyms

MMRRC Submission

042554-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4957 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

131028869-131040417 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 131035525 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 341 (V341A)

Ref Sequence

ENSEMBL: ENSMUSP00000078784 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028804] [ENSMUST00000079857]

AlphaFold

P30306

Predicted Effect

possibly damaging
Transcript: ENSMUST00000028804
AA Change: V367A

PolyPhen 2 Score 0.456 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000028804
Gene: ENSMUSG00000027330
AA Change: V367A

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	379	3.3e-103	PFAM
RHOD	417	531	4.29e-26	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000079857
AA Change: V341A

PolyPhen 2 Score 0.801 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000078784
Gene: ENSMUSG00000027330
AA Change: V341A

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	354	2.4e-78	PFAM
RHOD	391	505	4.29e-26	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125423

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132727

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138669

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141732

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145556

Meta Mutation Damage Score

0.1452

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.5%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
PHENOTYPE: The resumption of meiosis during oocyte maturation is blocked in homozygous mutant female mice, resulting in female infertility. Male mice do not show an overt reproductive phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110009E18Rik	G	C	1: 120,096,840 (GRCm39)		probably benign	Het
3110009E18Rik	C	T	1: 120,096,850 (GRCm39)		probably benign	Het
3110009E18Rik	G	T	1: 120,096,849 (GRCm39)		probably benign	Het
6820408C15Rik	T	A	2: 152,286,013 (GRCm39)	V342D	probably damaging	Het
Arhgap33	C	T	7: 30,231,786 (GRCm39)	G101R	probably damaging	Het
Atf7ip	C	T	6: 136,583,808 (GRCm39)	R1280C	probably damaging	Het
Cacnb4	T	C	2: 52,448,303 (GRCm39)	H8R	probably damaging	Het
Ccdc150	T	A	1: 54,404,027 (GRCm39)		probably benign	Het
Cd74	A	G	18: 60,942,109 (GRCm39)	N113D	probably benign	Het
Clptm1l	T	A	13: 73,759,315 (GRCm39)	I245N	possibly damaging	Het
Clptm1l	T	C	13: 73,760,547 (GRCm39)	I310T	probably damaging	Het
Creb5	A	T	6: 53,670,907 (GRCm39)		probably null	Het
Crebbp	T	C	16: 3,935,231 (GRCm39)	Q460R	probably benign	Het
Dnah17	A	T	11: 117,965,124 (GRCm39)	I2306N	probably benign	Het
Dync1h1	C	A	12: 110,624,560 (GRCm39)	T3700N	probably damaging	Het
Elovl1	A	T	4: 118,289,120 (GRCm39)	H215L	probably damaging	Het
Enkd1	A	G	8: 106,431,121 (GRCm39)	I202T	probably benign	Het
Epha7	C	T	4: 28,871,892 (GRCm39)	A407V	probably damaging	Het
Ercc3	G	T	18: 32,376,170 (GRCm39)	G130W	probably damaging	Het
Fbxl8	A	G	8: 105,994,827 (GRCm39)	E113G	probably damaging	Het
Frmpd1	T	G	4: 45,273,099 (GRCm39)	N339K	probably damaging	Het
Frrs1	G	A	3: 116,678,897 (GRCm39)	D240N	probably benign	Het
Gemin2	A	G	12: 59,063,954 (GRCm39)	S105G	probably benign	Het
Glra1	T	A	11: 55,418,224 (GRCm39)	I257F	probably damaging	Het
Gmcl1	G	A	6: 86,687,503 (GRCm39)	P354S	probably damaging	Het
Gpr15	G	T	16: 58,538,537 (GRCm39)	A184E	probably damaging	Het
Grm7	G	A	6: 111,335,824 (GRCm39)	G745E	probably damaging	Het
H2-T10	T	A	17: 36,428,308 (GRCm39)		probably benign	Het
Hdac5	C	A	11: 102,096,082 (GRCm39)		probably benign	Het
Ift22	G	A	5: 136,937,070 (GRCm39)		probably benign	Het
Ighg3	T	C	12: 113,324,750 (GRCm39)	E34G	unknown	Het
Itga11	A	T	9: 62,674,930 (GRCm39)	T821S	probably benign	Het
Lmod2	T	C	6: 24,603,871 (GRCm39)	V282A	possibly damaging	Het
Maml2	A	G	9: 13,531,572 (GRCm39)	K262R	probably damaging	Het
Mme	T	A	3: 63,250,910 (GRCm39)		probably benign	Het
Mnat1	A	G	12: 73,170,652 (GRCm39)	Y14C	probably damaging	Het
Mtdh	A	T	15: 34,083,281 (GRCm39)	T34S	possibly damaging	Het
Ncaph	T	C	2: 126,963,177 (GRCm39)	D352G	possibly damaging	Het
Or2t43	A	G	11: 58,457,344 (GRCm39)	Y276H	probably damaging	Het
Or4f17-ps1	C	A	2: 111,358,569 (GRCm39)	N321K	probably benign	Het
Or5m9b	T	A	2: 85,905,854 (GRCm39)	Y257N	probably damaging	Het
Or9g8	T	C	2: 85,607,459 (GRCm39)	F177S	probably damaging	Het
Pcdhb13	T	A	18: 37,577,837 (GRCm39)	D738E	possibly damaging	Het
Pla2g4f	C	T	2: 120,130,980 (GRCm39)	R825Q	probably benign	Het
Pnliprp2	C	T	19: 58,763,577 (GRCm39)	L409F	possibly damaging	Het
Prlr	G	T	15: 10,319,281 (GRCm39)	C70F	probably damaging	Het
Ptpn14	C	T	1: 189,583,469 (GRCm39)	T772I	probably benign	Het
Ryr2	T	C	13: 11,799,966 (GRCm39)	Q927R	probably damaging	Het
Scarf1	A	G	11: 75,416,460 (GRCm39)	E634G	probably benign	Het
Scart1	T	C	7: 139,808,435 (GRCm39)	V782A	probably damaging	Het
Skic3	T	G	13: 76,333,232 (GRCm39)		probably null	Het
Slc39a14	A	T	14: 70,553,260 (GRCm39)	S158R	probably damaging	Het
Slc9c1	A	T	16: 45,365,194 (GRCm39)	T176S	probably benign	Het
Srsf10	T	C	4: 135,583,541 (GRCm39)	S2P	probably damaging	Het
Tcam1	T	A	11: 106,173,705 (GRCm39)	C50S	probably damaging	Het
Tcf7l2	A	G	19: 55,919,864 (GRCm39)		probably null	Het
Tdrd3	T	A	14: 87,743,223 (GRCm39)	H390Q	probably benign	Het
Tlk2	T	C	11: 105,144,185 (GRCm39)		probably null	Het
Tnc	G	C	4: 63,894,793 (GRCm39)	P1531R	probably damaging	Het
Tnfrsf1b	C	A	4: 144,973,327 (GRCm39)	Q15H	probably damaging	Het
Tnfrsf1b	T	G	4: 144,973,328 (GRCm39)	Q15P	possibly damaging	Het
Tssk4	A	T	14: 55,889,266 (GRCm39)	E264V	probably damaging	Het
Ugt3a1	G	A	15: 9,365,274 (GRCm39)	V296I	probably benign	Het
Usp5	C	G	6: 124,799,593 (GRCm39)	K318N	possibly damaging	Het
Vmn2r8	T	G	5: 108,947,129 (GRCm39)	E541A	probably benign	Het
Ybx1	A	T	4: 119,136,135 (GRCm39)		probably benign	Het
Zfp39	T	A	11: 58,782,057 (GRCm39)	Y235F	possibly damaging	Het
Zfp422	T	A	6: 116,603,904 (GRCm39)	K32*	probably null	Het
Zpbp2	T	A	11: 98,442,150 (GRCm39)		probably null	Het

Other mutations in Cdc25b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03230:Cdc25b	APN	2	131,030,060 (GRCm39)	missense	probably benign	0.00
R0471:Cdc25b	UTSW	2	131,039,204 (GRCm39)	missense	probably damaging	0.99
R0639:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R0645:Cdc25b	UTSW	2	131,033,533 (GRCm39)	missense	probably benign	0.06
R0673:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R1574:Cdc25b	UTSW	2	131,033,057 (GRCm39)	splice site	probably benign
R4094:Cdc25b	UTSW	2	131,031,037 (GRCm39)	missense	probably benign
R4433:Cdc25b	UTSW	2	131,033,618 (GRCm39)	missense	probably benign	0.02
R4722:Cdc25b	UTSW	2	131,035,271 (GRCm39)	missense	probably damaging	1.00
R4817:Cdc25b	UTSW	2	131,035,223 (GRCm39)	missense	probably damaging	1.00
R5345:Cdc25b	UTSW	2	131,034,516 (GRCm39)	missense	probably benign	0.18
R5407:Cdc25b	UTSW	2	131,035,567 (GRCm39)	missense	probably damaging	1.00
R5562:Cdc25b	UTSW	2	131,036,678 (GRCm39)	missense	probably damaging	1.00
R5594:Cdc25b	UTSW	2	131,033,538 (GRCm39)	missense	probably damaging	1.00
R5792:Cdc25b	UTSW	2	131,033,679 (GRCm39)	missense	probably damaging	1.00
R5831:Cdc25b	UTSW	2	131,029,301 (GRCm39)	critical splice donor site	probably null
R7204:Cdc25b	UTSW	2	131,033,552 (GRCm39)	missense	probably damaging	1.00
R7292:Cdc25b	UTSW	2	131,033,093 (GRCm39)	missense	probably damaging	1.00
R7399:Cdc25b	UTSW	2	131,036,574 (GRCm39)	missense	probably damaging	1.00
R7501:Cdc25b	UTSW	2	131,036,080 (GRCm39)	missense	probably damaging	1.00
R7772:Cdc25b	UTSW	2	131,031,029 (GRCm39)	missense	probably damaging	1.00
R8186:Cdc25b	UTSW	2	131,031,050 (GRCm39)	missense	probably benign	0.05
R8783:Cdc25b	UTSW	2	131,033,772 (GRCm39)	missense	probably benign	0.05
R8985:Cdc25b	UTSW	2	131,035,180 (GRCm39)	missense	probably damaging	1.00
R9141:Cdc25b	UTSW	2	131,033,857 (GRCm39)	critical splice donor site	probably null
R9153:Cdc25b	UTSW	2	131,034,564 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- CGGTCACTGAAGATGCTCTG -3'
(R):5'- ACAGATTTGAGGTGGCATCAG -3'

Sequencing Primer
(F):5'- CACTGAAGATGCTCTGGGTGTACC -3'
(R):5'- TTTGAGGTGGCATCAGAAAGG -3'

Posted On

2016-04-27