Mutagenetix > Incidental Mutations

Incidental Mutation 'R4957:Cdc25b'

381629

Institutional Source

Beutler Lab

Gene Symbol

Cdc25b

Ensembl Gene

ENSMUSG00000027330

Gene Name

cell division cycle 25B

Synonyms

MMRRC Submission

042554-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4957 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

131186949-131198497 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 131193605 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 341 (V341A)

Ref Sequence

ENSEMBL: ENSMUSP00000078784 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028804] [ENSMUST00000079857]

AlphaFold

P30306

Predicted Effect

possibly damaging
Transcript: ENSMUST00000028804
AA Change: V367A

PolyPhen 2 Score 0.456 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000028804
Gene: ENSMUSG00000027330
AA Change: V367A

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	379	3.3e-103	PFAM
RHOD	417	531	4.29e-26	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000079857
AA Change: V341A

PolyPhen 2 Score 0.801 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000078784
Gene: ENSMUSG00000027330
AA Change: V341A

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	354	2.4e-78	PFAM
RHOD	391	505	4.29e-26	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125423

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132727

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138669

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141732

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145556

Meta Mutation Damage Score

0.1452

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.5%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
PHENOTYPE: The resumption of meiosis during oocyte maturation is blocked in homozygous mutant female mice, resulting in female infertility. Male mice do not show an overt reproductive phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110009E18Rik	G	C	1: 120,169,110		probably benign	Het
3110009E18Rik	G	T	1: 120,169,119		probably benign	Het
3110009E18Rik	C	T	1: 120,169,120		probably benign	Het
6820408C15Rik	T	A	2: 152,444,093	V342D	probably damaging	Het
Arhgap33	C	T	7: 30,532,361	G101R	probably damaging	Het
Atf7ip	C	T	6: 136,606,810	R1280C	probably damaging	Het
Cacnb4	T	C	2: 52,558,291	H8R	probably damaging	Het
Ccdc150	T	A	1: 54,364,868		probably benign	Het
Cd163l1	T	C	7: 140,228,522	V782A	probably damaging	Het
Cd74	A	G	18: 60,809,037	N113D	probably benign	Het
Clptm1l	T	A	13: 73,611,196	I245N	possibly damaging	Het
Clptm1l	T	C	13: 73,612,428	I310T	probably damaging	Het
Creb5	A	T	6: 53,693,922		probably null	Het
Crebbp	T	C	16: 4,117,367	Q460R	probably benign	Het
Dnah17	A	T	11: 118,074,298	I2306N	probably benign	Het
Dync1h1	C	A	12: 110,658,126	T3700N	probably damaging	Het
Elovl1	A	T	4: 118,431,923	H215L	probably damaging	Het
Enkd1	A	G	8: 105,704,489	I202T	probably benign	Het
Epha7	C	T	4: 28,871,892	A407V	probably damaging	Het
Ercc3	G	T	18: 32,243,117	G130W	probably damaging	Het
Fbxl8	A	G	8: 105,268,195	E113G	probably damaging	Het
Frmpd1	T	G	4: 45,273,099	N339K	probably damaging	Het
Frrs1	G	A	3: 116,885,248	D240N	probably benign	Het
Gemin2	A	G	12: 59,017,168	S105G	probably benign	Het
Glra1	T	A	11: 55,527,398	I257F	probably damaging	Het
Gmcl1	G	A	6: 86,710,521	P354S	probably damaging	Het
Gpr15	G	T	16: 58,718,174	A184E	probably damaging	Het
Grm7	G	A	6: 111,358,863	G745E	probably damaging	Het
H2-T10	T	A	17: 36,117,416		probably benign	Het
Hdac5	C	A	11: 102,205,256		probably benign	Het
Ift22	G	A	5: 136,908,216		probably benign	Het
Ighg3	T	C	12: 113,361,130	E34G	unknown	Het
Itga11	A	T	9: 62,767,648	T821S	probably benign	Het
Lmod2	T	C	6: 24,603,872	V282A	possibly damaging	Het
Maml2	A	G	9: 13,620,276	K262R	probably damaging	Het
Mme	T	A	3: 63,343,489		probably benign	Het
Mnat1	A	G	12: 73,123,878	Y14C	probably damaging	Het
Mtdh	A	T	15: 34,083,135	T34S	possibly damaging	Het
Ncaph	T	C	2: 127,121,257	D352G	possibly damaging	Het
Olfr1014	T	C	2: 85,777,115	F177S	probably damaging	Het
Olfr1036	T	A	2: 86,075,510	Y257N	probably damaging	Het
Olfr1293-ps	C	A	2: 111,528,224	N321K	probably benign	Het
Olfr224	A	G	11: 58,566,518	Y276H	probably damaging	Het
Pcdhb13	T	A	18: 37,444,784	D738E	possibly damaging	Het
Pla2g4f	C	T	2: 120,300,499	R825Q	probably benign	Het
Pnliprp2	C	T	19: 58,775,145	L409F	possibly damaging	Het
Prlr	G	T	15: 10,319,195	C70F	probably damaging	Het
Ptpn14	C	T	1: 189,851,272	T772I	probably benign	Het
Ryr2	T	C	13: 11,785,080	Q927R	probably damaging	Het
Scarf1	A	G	11: 75,525,634	E634G	probably benign	Het
Slc39a14	A	T	14: 70,315,811	S158R	probably damaging	Het
Slc9c1	A	T	16: 45,544,831	T176S	probably benign	Het
Srsf10	T	C	4: 135,856,230	S2P	probably damaging	Het
Tcam1	T	A	11: 106,282,879	C50S	probably damaging	Het
Tcf7l2	A	G	19: 55,931,432		probably null	Het
Tdrd3	T	A	14: 87,505,787	H390Q	probably benign	Het
Tlk2	T	C	11: 105,253,359		probably null	Het
Tnc	G	C	4: 63,976,556	P1531R	probably damaging	Het
Tnfrsf1b	C	A	4: 145,246,757	Q15H	probably damaging	Het
Tnfrsf1b	T	G	4: 145,246,758	Q15P	possibly damaging	Het
Tssk4	A	T	14: 55,651,809	E264V	probably damaging	Het
Ttc37	T	G	13: 76,185,113		probably null	Het
Ugt3a2	G	A	15: 9,365,188	V296I	probably benign	Het
Usp5	C	G	6: 124,822,630	K318N	possibly damaging	Het
Vmn2r8	T	G	5: 108,799,263	E541A	probably benign	Het
Ybx1	A	T	4: 119,278,938		probably benign	Het
Zfp39	T	A	11: 58,891,231	Y235F	possibly damaging	Het
Zfp422	T	A	6: 116,626,943	K32*	probably null	Het
Zpbp2	T	A	11: 98,551,324		probably null	Het

Other mutations in Cdc25b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03230:Cdc25b	APN	2	131188140	missense	probably benign	0.00
R0471:Cdc25b	UTSW	2	131197284	missense	probably damaging	0.99
R0639:Cdc25b	UTSW	2	131197262	missense	probably benign	0.00
R0645:Cdc25b	UTSW	2	131191613	missense	probably benign	0.06
R0673:Cdc25b	UTSW	2	131197262	missense	probably benign	0.00
R1574:Cdc25b	UTSW	2	131191137	splice site	probably benign
R4094:Cdc25b	UTSW	2	131189117	missense	probably benign
R4433:Cdc25b	UTSW	2	131191698	missense	probably benign	0.02
R4722:Cdc25b	UTSW	2	131193351	missense	probably damaging	1.00
R4817:Cdc25b	UTSW	2	131193303	missense	probably damaging	1.00
R5345:Cdc25b	UTSW	2	131192596	missense	probably benign	0.18
R5407:Cdc25b	UTSW	2	131193647	missense	probably damaging	1.00
R5562:Cdc25b	UTSW	2	131194758	missense	probably damaging	1.00
R5594:Cdc25b	UTSW	2	131191618	missense	probably damaging	1.00
R5792:Cdc25b	UTSW	2	131191759	missense	probably damaging	1.00
R5831:Cdc25b	UTSW	2	131187381	critical splice donor site	probably null
R7204:Cdc25b	UTSW	2	131191632	missense	probably damaging	1.00
R7292:Cdc25b	UTSW	2	131191173	missense	probably damaging	1.00
R7399:Cdc25b	UTSW	2	131194654	missense	probably damaging	1.00
R7501:Cdc25b	UTSW	2	131194160	missense	probably damaging	1.00
R7772:Cdc25b	UTSW	2	131189109	missense	probably damaging	1.00
R8186:Cdc25b	UTSW	2	131189130	missense	probably benign	0.05
R8783:Cdc25b	UTSW	2	131191852	missense	probably benign	0.05
R8985:Cdc25b	UTSW	2	131193260	missense	probably damaging	1.00
R9141:Cdc25b	UTSW	2	131191937	critical splice donor site	probably null
R9153:Cdc25b	UTSW	2	131192644	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- CGGTCACTGAAGATGCTCTG -3'
(R):5'- ACAGATTTGAGGTGGCATCAG -3'

Sequencing Primer
(F):5'- CACTGAAGATGCTCTGGGTGTACC -3'
(R):5'- TTTGAGGTGGCATCAGAAAGG -3'

Posted On

2016-04-27