Mutagenetix > Incidental Mutation

Incidental Mutation 'R4950:Zfp607a'

381915

Institutional Source

Beutler Lab

Gene Symbol

Zfp607a

Ensembl Gene

ENSMUSG00000020420

Gene Name

zinc finger protein 607A

Synonyms

Zfp607, 4732475C15Rik

MMRRC Submission

042547-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R4950 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

27857527-27880825 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 27878751 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 415 (H415Q)

Ref Sequence

ENSEMBL: ENSMUSP00000146006 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053722] [ENSMUST00000205534] [ENSMUST00000205715]

AlphaFold

Q3TQG9

Predicted Effect

probably damaging
Transcript: ENSMUST00000053722
AA Change: H415Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000051496
Gene: ENSMUSG00000020420
AA Change: H415Q

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.91e-2	SMART
ZnF_C2H2	201	223	3.44e-4	SMART
ZnF_C2H2	229	251	3.83e-2	SMART
ZnF_C2H2	257	279	4.87e-4	SMART
ZnF_C2H2	285	307	1.38e-3	SMART
ZnF_C2H2	313	335	4.17e-3	SMART
ZnF_C2H2	341	363	2.86e-1	SMART
ZnF_C2H2	369	391	5.14e-3	SMART
ZnF_C2H2	397	419	1.2e-3	SMART
ZnF_C2H2	425	447	4.47e-3	SMART
ZnF_C2H2	453	475	1.1e-2	SMART
ZnF_C2H2	481	503	1.45e-2	SMART
ZnF_C2H2	509	531	1.12e-3	SMART
ZnF_C2H2	537	559	1.5e-4	SMART
ZnF_C2H2	565	587	8.34e-3	SMART
ZnF_C2H2	593	615	1.12e-3	SMART
ZnF_C2H2	621	643	6.42e-4	SMART
ZnF_C2H2	649	671	6.23e-2	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000205534
AA Change: H415Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000205715
AA Change: H415Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000206136

Meta Mutation Damage Score

0.2940

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.5%
20x: 92.9%

Validation Efficiency

100% (58/58)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bpifb9b	A	T	2: 154,311,659 (GRCm38)	D215V	probably damaging	Het
Cacna1i	A	G	15: 80,368,671 (GRCm38)	E625G	probably damaging	Het
Cage1	C	T	13: 38,023,326 (GRCm38)	S181N	possibly damaging	Het
Ccdc73	A	C	2: 104,992,366 (GRCm38)	I887L	probably benign	Het
Cfap65	T	A	1: 74,906,336 (GRCm38)	K1408*	probably null	Het
Cngb1	C	A	8: 95,248,507 (GRCm38)	G654W	probably damaging	Het
Cxcl15	A	T	5: 90,795,245 (GRCm38)	E35D	possibly damaging	Het
Ddi1	A	G	9: 6,266,073 (GRCm38)	S99P	probably benign	Het
Disp3	A	T	4: 148,258,126 (GRCm38)	D622E	possibly damaging	Het
Dnajc22	G	A	15: 99,101,734 (GRCm38)	V267I	probably benign	Het
Dph5	G	A	3: 115,928,643 (GRCm38)	G257S	probably benign	Het
Elmo2	A	T	2: 165,314,813 (GRCm38)		probably null	Het
Fam186a	T	C	15: 99,941,653 (GRCm38)	R2237G	unknown	Het
Fez1	G	A	9: 36,867,882 (GRCm38)	R285Q	probably damaging	Het
Fsip2	A	T	2: 82,946,932 (GRCm38)	H101L	probably damaging	Het
Fsip2	G	A	2: 82,977,414 (GRCm38)	C1359Y	probably benign	Het
Fxyd7	G	A	7: 31,047,390 (GRCm38)	T15I	probably benign	Het
Gpr155	A	T	2: 73,382,185 (GRCm38)	D31E	probably benign	Het
Iho1	A	C	9: 108,421,510 (GRCm38)	S36R	probably damaging	Het
Irx6	A	T	8: 92,678,800 (GRCm38)	Y432F	probably damaging	Het
Itih5	A	C	2: 10,235,081 (GRCm38)	I340L	probably damaging	Het
Lao1	T	C	4: 118,965,375 (GRCm38)	L164S	probably damaging	Het
Mcoln3	T	C	3: 146,139,519 (GRCm38)	I490T	probably damaging	Het
Mef2b	T	C	8: 70,167,196 (GRCm38)	Y311H	probably damaging	Het
Mras	A	T	9: 99,394,484 (GRCm38)	L111Q	probably damaging	Het
Mrc1	A	G	2: 14,271,280 (GRCm38)	D475G	probably damaging	Het
Nrcam	T	C	12: 44,598,490 (GRCm38)	I1155T	probably damaging	Het
Ntrk1	A	G	3: 87,789,611 (GRCm38)		probably null	Het
Or7e165	G	A	9: 19,783,731 (GRCm38)	M199I	probably benign	Het
Or8b38	T	C	9: 38,062,001 (GRCm38)	I227T	probably damaging	Het
Parp9	A	T	16: 35,948,007 (GRCm38)	I186F	probably damaging	Het
Pcdhac2	T	A	18: 37,145,230 (GRCm38)	V421E	probably benign	Het
Pck1	T	A	2: 173,154,827 (GRCm38)	I178K	probably benign	Het
Pde6b	A	G	5: 108,430,703 (GRCm38)	K836E	probably benign	Het
Ptprd	T	A	4: 76,140,515 (GRCm38)		probably null	Het
Pwp2	A	G	10: 78,183,006 (GRCm38)	Y56H	probably benign	Het
Rarb	G	A	14: 16,432,085 (GRCm38)		probably benign	Het
Rpain	C	G	11: 70,970,921 (GRCm38)	H50Q	probably benign	Het
Rps9	CTGTTTG	CTG	7: 3,704,759 (GRCm38)		probably null	Het
Rubcn	A	T	16: 32,843,193 (GRCm38)	S358T	probably damaging	Het
Ryr2	G	A	13: 11,742,011 (GRCm38)	R1586C	probably damaging	Het
Slfn9	T	C	11: 82,981,904 (GRCm38)	I669V	probably benign	Het
Slx1b	T	C	7: 126,691,767 (GRCm38)		probably benign	Het
Spsb3	T	A	17: 24,887,511 (GRCm38)		probably benign	Het
Tbx15	A	G	3: 99,326,384 (GRCm38)	I288V	possibly damaging	Het
Tlr2	C	A	3: 83,837,332 (GRCm38)	E481D	probably damaging	Het
Trim17	A	T	11: 58,970,428 (GRCm38)	D253V	probably damaging	Het
Trim69	A	G	2: 122,178,746 (GRCm38)	D429G	probably damaging	Het
Vstm5	A	G	9: 15,257,794 (GRCm38)		probably null	Het
Vwa3b	A	G	1: 37,085,332 (GRCm38)	Q337R	probably benign	Het
Zfp113	T	C	5: 138,145,472 (GRCm38)	N172S	probably benign	Het
Zfp87	G	A	13: 67,517,899 (GRCm38)	T148I	probably benign	Het

Other mutations in Zfp607a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00164:Zfp607a	APN	7	27,877,789 (GRCm38)	missense	possibly damaging	0.55
IGL01019:Zfp607a	APN	7	27,878,617 (GRCm38)	missense	probably damaging	1.00
IGL01412:Zfp607a	APN	7	27,878,684 (GRCm38)	missense	probably damaging	0.99
IGL03206:Zfp607a	APN	7	27,877,823 (GRCm38)	missense	possibly damaging	0.52
R0071:Zfp607a	UTSW	7	27,878,269 (GRCm38)	missense	probably damaging	0.96
R0304:Zfp607a	UTSW	7	27,879,212 (GRCm38)	missense	possibly damaging	0.92
R0685:Zfp607a	UTSW	7	27,878,476 (GRCm38)	missense	probably damaging	0.97
R0726:Zfp607a	UTSW	7	27,879,149 (GRCm38)	missense	probably benign	0.00
R1201:Zfp607a	UTSW	7	27,879,311 (GRCm38)	missense	probably damaging	1.00
R1304:Zfp607a	UTSW	7	27,865,575 (GRCm38)	missense	probably benign	0.00
R1648:Zfp607a	UTSW	7	27,879,068 (GRCm38)	missense	probably benign	0.02
R1732:Zfp607a	UTSW	7	27,878,459 (GRCm38)	missense	probably damaging	1.00
R2194:Zfp607a	UTSW	7	27,879,380 (GRCm38)	missense	possibly damaging	0.73
R3793:Zfp607a	UTSW	7	27,878,906 (GRCm38)	missense	probably benign	0.01
R3808:Zfp607a	UTSW	7	27,879,401 (GRCm38)	missense	probably benign	0.01
R4296:Zfp607a	UTSW	7	27,865,648 (GRCm38)	missense	probably damaging	1.00
R4786:Zfp607a	UTSW	7	27,879,413 (GRCm38)	missense	probably damaging	1.00
R4792:Zfp607a	UTSW	7	27,878,653 (GRCm38)	missense	probably benign	0.23
R4915:Zfp607a	UTSW	7	27,878,560 (GRCm38)	missense	probably benign	0.00
R5123:Zfp607a	UTSW	7	27,879,098 (GRCm38)	missense	probably damaging	1.00
R5217:Zfp607a	UTSW	7	27,877,844 (GRCm38)	missense	probably damaging	0.97
R5270:Zfp607a	UTSW	7	27,878,305 (GRCm38)	nonsense	probably null
R5403:Zfp607a	UTSW	7	27,879,319 (GRCm38)	missense	possibly damaging	0.54
R6010:Zfp607a	UTSW	7	27,877,829 (GRCm38)	nonsense	probably null
R6224:Zfp607a	UTSW	7	27,878,582 (GRCm38)	missense	probably damaging	1.00
R6939:Zfp607a	UTSW	7	27,879,048 (GRCm38)	nonsense	probably null
R6953:Zfp607a	UTSW	7	27,878,365 (GRCm38)	missense	possibly damaging	0.59
R7082:Zfp607a	UTSW	7	27,878,758 (GRCm38)	missense	probably damaging	1.00
R7781:Zfp607a	UTSW	7	27,865,575 (GRCm38)	missense	possibly damaging	0.90
R7909:Zfp607a	UTSW	7	27,879,094 (GRCm38)	missense	probably damaging	1.00
R8191:Zfp607a	UTSW	7	27,879,443 (GRCm38)	missense	possibly damaging	0.93
R8224:Zfp607a	UTSW	7	27,878,111 (GRCm38)	missense	probably damaging	1.00
R8949:Zfp607a	UTSW	7	27,878,519 (GRCm38)	missense	possibly damaging	0.56
R8962:Zfp607a	UTSW	7	27,879,361 (GRCm38)	missense	possibly damaging	0.79
R9178:Zfp607a	UTSW	7	27,877,957 (GRCm38)	missense	probably benign	0.00
R9802:Zfp607a	UTSW	7	27,879,279 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCGGCACCAGAGTATTCATAC -3'
(R):5'- ACTGGGAAGATGAAAAGCCTTTC -3'

Sequencing Primer
(F):5'- CCAATGAGAAGCCCTTTGAATG -3'
(R):5'- CACCACTGTGAGATTTCTGATG -3'

Posted On

2016-04-27