Incidental Mutation 'R4950:Zfp87'
| ID |
381935 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp87
|
| Ensembl Gene |
ENSMUSG00000097333 |
| Gene Name |
zinc finger protein 87 |
| Synonyms |
Mzf22, 2210039O17Rik, 4631412O18Rik, Zfp72, KRAB4 |
| MMRRC Submission |
042547-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.116)
|
| Stock # |
R4950 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
13 |
| Chromosomal Location |
67663900-67674296 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to A
at 67666018 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Isoleucine
at position 148
(T148I)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000138087
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000167914]
[ENSMUST00000180536]
[ENSMUST00000181341]
[ENSMUST00000181573]
[ENSMUST00000181767]
|
| AlphaFold |
Q8K2A4 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000167914
|
| SMART Domains |
Protein: ENSMUSP00000137830
Gene: ENSMUSG00000097565
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
4.41e-34 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000180536
|
| SMART Domains |
Protein: ENSMUSP00000137812
Gene: ENSMUSG00000097333
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
51 |
7.02e-19 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000181341
|
| SMART Domains |
Protein: ENSMUSP00000137689
Gene: ENSMUSG00000097333
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
4.41e-34 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000181573
|
| SMART Domains |
Protein: ENSMUSP00000138011
Gene: ENSMUSG00000097333
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
51 |
7.02e-19 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000181767
AA Change: T148I
PolyPhen 2
Score 0.202 (Sensitivity: 0.92; Specificity: 0.88)
|
| SMART Domains |
Protein: ENSMUSP00000138087
Gene: ENSMUSG00000097333
AA Change: T148I
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
4.41e-34 |
SMART |
|
ZnF_C2H2
|
109 |
131 |
2.61e-4 |
SMART |
|
ZnF_C2H2
|
137 |
159 |
9.22e-5 |
SMART |
|
ZnF_C2H2
|
165 |
187 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
193 |
215 |
7.15e-2 |
SMART |
|
ZnF_C2H2
|
221 |
243 |
9.22e-5 |
SMART |
|
ZnF_C2H2
|
249 |
271 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
6.42e-4 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
3.69e-4 |
SMART |
|
ZnF_C2H2
|
417 |
439 |
5.9e-3 |
SMART |
|
ZnF_C2H2
|
445 |
467 |
3.21e-4 |
SMART |
|
ZnF_C2H2
|
472 |
494 |
9.08e-4 |
SMART |
|
ZnF_C2H2
|
500 |
522 |
4.47e-3 |
SMART |
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.3%
- 10x: 96.5%
- 20x: 92.9%
|
| Validation Efficiency |
100% (58/58) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 52 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Bpifb9b |
A |
T |
2: 154,153,579 (GRCm39) |
D215V |
probably damaging |
Het |
| Cacna1i |
A |
G |
15: 80,252,872 (GRCm39) |
E625G |
probably damaging |
Het |
| Cage1 |
C |
T |
13: 38,207,302 (GRCm39) |
S181N |
possibly damaging |
Het |
| Ccdc73 |
A |
C |
2: 104,822,711 (GRCm39) |
I887L |
probably benign |
Het |
| Cfap65 |
T |
A |
1: 74,945,495 (GRCm39) |
K1408* |
probably null |
Het |
| Cngb1 |
C |
A |
8: 95,975,135 (GRCm39) |
G654W |
probably damaging |
Het |
| Cxcl15 |
A |
T |
5: 90,943,104 (GRCm39) |
E35D |
possibly damaging |
Het |
| Ddi1 |
A |
G |
9: 6,266,073 (GRCm39) |
S99P |
probably benign |
Het |
| Disp3 |
A |
T |
4: 148,342,583 (GRCm39) |
D622E |
possibly damaging |
Het |
| Dnajc22 |
G |
A |
15: 98,999,615 (GRCm39) |
V267I |
probably benign |
Het |
| Dph5 |
G |
A |
3: 115,722,292 (GRCm39) |
G257S |
probably benign |
Het |
| Elmo2 |
A |
T |
2: 165,156,733 (GRCm39) |
|
probably null |
Het |
| Fam186a |
T |
C |
15: 99,839,534 (GRCm39) |
R2237G |
unknown |
Het |
| Fez1 |
G |
A |
9: 36,779,178 (GRCm39) |
R285Q |
probably damaging |
Het |
| Fsip2 |
A |
T |
2: 82,777,276 (GRCm39) |
H101L |
probably damaging |
Het |
| Fsip2 |
G |
A |
2: 82,807,758 (GRCm39) |
C1359Y |
probably benign |
Het |
| Fxyd7 |
G |
A |
7: 30,746,815 (GRCm39) |
T15I |
probably benign |
Het |
| Gpr155 |
A |
T |
2: 73,212,529 (GRCm39) |
D31E |
probably benign |
Het |
| Iho1 |
A |
C |
9: 108,298,709 (GRCm39) |
S36R |
probably damaging |
Het |
| Irx6 |
A |
T |
8: 93,405,428 (GRCm39) |
Y432F |
probably damaging |
Het |
| Itih5 |
A |
C |
2: 10,239,892 (GRCm39) |
I340L |
probably damaging |
Het |
| Lao1 |
T |
C |
4: 118,822,572 (GRCm39) |
L164S |
probably damaging |
Het |
| Mcoln3 |
T |
C |
3: 145,845,274 (GRCm39) |
I490T |
probably damaging |
Het |
| Mef2b |
T |
C |
8: 70,619,846 (GRCm39) |
Y311H |
probably damaging |
Het |
| Mras |
A |
T |
9: 99,276,537 (GRCm39) |
L111Q |
probably damaging |
Het |
| Mrc1 |
A |
G |
2: 14,276,091 (GRCm39) |
D475G |
probably damaging |
Het |
| Nrcam |
T |
C |
12: 44,645,273 (GRCm39) |
I1155T |
probably damaging |
Het |
| Ntrk1 |
A |
G |
3: 87,696,918 (GRCm39) |
|
probably null |
Het |
| Or7e165 |
G |
A |
9: 19,695,027 (GRCm39) |
M199I |
probably benign |
Het |
| Or8b38 |
T |
C |
9: 37,973,297 (GRCm39) |
I227T |
probably damaging |
Het |
| Parp9 |
A |
T |
16: 35,768,377 (GRCm39) |
I186F |
probably damaging |
Het |
| Pcdhac2 |
T |
A |
18: 37,278,283 (GRCm39) |
V421E |
probably benign |
Het |
| Pck1 |
T |
A |
2: 172,996,620 (GRCm39) |
I178K |
probably benign |
Het |
| Pde6b |
A |
G |
5: 108,578,569 (GRCm39) |
K836E |
probably benign |
Het |
| Ptprd |
T |
A |
4: 76,058,752 (GRCm39) |
|
probably null |
Het |
| Pwp2 |
A |
G |
10: 78,018,840 (GRCm39) |
Y56H |
probably benign |
Het |
| Rarb |
G |
A |
14: 16,432,085 (GRCm38) |
|
probably benign |
Het |
| Rpain |
C |
G |
11: 70,861,747 (GRCm39) |
H50Q |
probably benign |
Het |
| Rps9 |
CTGTTTG |
CTG |
7: 3,707,758 (GRCm39) |
|
probably null |
Het |
| Rubcn |
A |
T |
16: 32,663,563 (GRCm39) |
S358T |
probably damaging |
Het |
| Ryr2 |
G |
A |
13: 11,756,897 (GRCm39) |
R1586C |
probably damaging |
Het |
| Slfn9 |
T |
C |
11: 82,872,730 (GRCm39) |
I669V |
probably benign |
Het |
| Slx1b |
T |
C |
7: 126,290,939 (GRCm39) |
|
probably benign |
Het |
| Spsb3 |
T |
A |
17: 25,106,485 (GRCm39) |
|
probably benign |
Het |
| Tbx15 |
A |
G |
3: 99,233,700 (GRCm39) |
I288V |
possibly damaging |
Het |
| Tlr2 |
C |
A |
3: 83,744,639 (GRCm39) |
E481D |
probably damaging |
Het |
| Trim17 |
A |
T |
11: 58,861,254 (GRCm39) |
D253V |
probably damaging |
Het |
| Trim69 |
A |
G |
2: 122,009,227 (GRCm39) |
D429G |
probably damaging |
Het |
| Vstm5 |
A |
G |
9: 15,169,090 (GRCm39) |
|
probably null |
Het |
| Vwa3b |
A |
G |
1: 37,124,413 (GRCm39) |
Q337R |
probably benign |
Het |
| Zfp113 |
T |
C |
5: 138,143,734 (GRCm39) |
N172S |
probably benign |
Het |
| Zfp607a |
T |
A |
7: 27,578,176 (GRCm39) |
H415Q |
probably damaging |
Het |
|
| Other mutations in Zfp87 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02165:Zfp87
|
APN |
13 |
67,665,989 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02372:Zfp87
|
APN |
13 |
67,668,739 (GRCm39) |
splice site |
probably benign |
|
|
IGL03160:Zfp87
|
APN |
13 |
67,669,392 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0653:Zfp87
|
UTSW |
13 |
74,520,190 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0711:Zfp87
|
UTSW |
13 |
74,524,544 (GRCm39) |
splice site |
probably benign |
|
|
R1498:Zfp87
|
UTSW |
13 |
74,520,736 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3801:Zfp87
|
UTSW |
13 |
67,669,334 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4032:Zfp87
|
UTSW |
13 |
74,520,449 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R4629:Zfp87
|
UTSW |
13 |
74,520,512 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5604:Zfp87
|
UTSW |
13 |
67,665,945 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6111:Zfp87
|
UTSW |
13 |
74,520,504 (GRCm39) |
missense |
probably benign |
0.31 |
|
R6130:Zfp87
|
UTSW |
13 |
74,520,460 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R6277:Zfp87
|
UTSW |
13 |
74,520,643 (GRCm39) |
nonsense |
probably null |
|
|
R6392:Zfp87
|
UTSW |
13 |
67,664,986 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6800:Zfp87
|
UTSW |
13 |
74,520,080 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6909:Zfp87
|
UTSW |
13 |
74,519,861 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7009:Zfp87
|
UTSW |
13 |
67,665,173 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7183:Zfp87
|
UTSW |
13 |
67,665,593 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7298:Zfp87
|
UTSW |
13 |
74,520,513 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R7330:Zfp87
|
UTSW |
13 |
74,523,153 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7341:Zfp87
|
UTSW |
13 |
74,520,467 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7448:Zfp87
|
UTSW |
13 |
67,665,163 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7597:Zfp87
|
UTSW |
13 |
67,665,412 (GRCm39) |
missense |
probably benign |
0.06 |
|
R8696:Zfp87
|
UTSW |
13 |
74,520,599 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9280:Zfp87
|
UTSW |
13 |
74,520,803 (GRCm39) |
missense |
probably benign |
0.16 |
|
R9429:Zfp87
|
UTSW |
13 |
74,520,703 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9780:Zfp87
|
UTSW |
13 |
67,665,241 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9782:Zfp87
|
UTSW |
13 |
74,520,932 (GRCm39) |
missense |
probably benign |
0.00 |
|
RF014:Zfp87
|
UTSW |
13 |
74,523,173 (GRCm39) |
missense |
probably benign |
0.17 |
|
Z1176:Zfp87
|
UTSW |
13 |
67,674,275 (GRCm39) |
start gained |
probably benign |
|
|
Z1177:Zfp87
|
UTSW |
13 |
74,519,911 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGGTCTGAGCTAGTTCTAAACATGC -3'
(R):5'- AATGTCGCTTGGAGCTCATTC -3'
Sequencing Primer
(F):5'- ACACCTGTAAGGATTCTCTGCAG -3'
(R):5'- CAAGACCTTTGATTGGAACTCACTC -3'
|
| Posted On |
2016-04-27 |
Incidental Mutation