Mutagenetix > Incidental Mutation

Incidental Mutation 'R4951:Rif1'

381953

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

042548-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4951 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 52084986 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000069794

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000112693

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.5%
20x: 93.1%

Validation Efficiency

98% (94/96)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy1	G	A	11: 7,138,336 (GRCm38)	E452K	possibly damaging	Het
Adcy10	T	C	1: 165,563,963 (GRCm38)	L1263P	probably damaging	Het
Adgrg1	G	A	8: 95,005,246 (GRCm38)	V179M	probably damaging	Het
Agap1	T	A	1: 89,609,503 (GRCm38)	V77E	probably damaging	Het
Ahnak	A	T	19: 9,017,835 (GRCm38)	K5494N	probably damaging	Het
Arhgap19	A	G	19: 41,774,106 (GRCm38)	M437T	probably benign	Het
C2cd4c	C	A	10: 79,613,005 (GRCm38)	A103S	possibly damaging	Het
Clip1	A	T	5: 123,630,345 (GRCm38)	D776E	probably benign	Het
Cntn3	C	T	6: 102,169,025 (GRCm38)	V952M	possibly damaging	Het
Col6a6	A	G	9: 105,767,198 (GRCm38)		probably null	Het
Crtac1	C	T	19: 42,414,131 (GRCm38)	A13T	probably benign	Het
Ddx50	C	T	10: 62,634,120 (GRCm38)	A363T	probably damaging	Het
Dock9	A	G	14: 121,653,135 (GRCm38)	V241A	probably benign	Het
Dysf	T	C	6: 84,114,120 (GRCm38)		probably null	Het
Enpp3	A	T	10: 24,798,277 (GRCm38)	M375K	probably damaging	Het
Fam13c	G	A	10: 70,551,791 (GRCm38)		probably null	Het
Ftcd	C	T	10: 76,584,683 (GRCm38)	A417V	probably benign	Het
Gak	A	G	5: 108,582,718 (GRCm38)	S941P	probably benign	Het
Ganc	A	G	2: 120,456,047 (GRCm38)	T786A	probably benign	Het
Gfi1	A	C	5: 107,720,143 (GRCm38)	S420A	probably damaging	Het
Ghsr	A	T	3: 27,372,361 (GRCm38)	T189S	possibly damaging	Het
Glb1l	C	T	1: 75,208,375 (GRCm38)	G122D	probably damaging	Het
Gm10065	C	T	13: 21,479,251 (GRCm38)	S64N	unknown	Het
Gm5087	C	A	14: 13,158,749 (GRCm38)		noncoding transcript	Het
Gm973	G	A	1: 59,541,474 (GRCm38)		probably null	Het
Gm9930	A	T	10: 9,534,705 (GRCm38)		noncoding transcript	Het
H6pd	A	T	4: 149,981,587 (GRCm38)	Y781N	probably damaging	Het
Ide	G	A	19: 37,285,232 (GRCm38)	L695F	unknown	Het
Il17re	T	A	6: 113,468,907 (GRCm38)	V393E	probably damaging	Het
Lipo1	A	G	19: 33,782,221 (GRCm38)	V205A	probably benign	Het
Lipo5	C	T	19: 33,468,851 (GRCm38)	E49K	probably damaging	Het
Lonp1	A	T	17: 56,620,335 (GRCm38)	M306K	possibly damaging	Het
Lrig1	A	G	6: 94,663,978 (GRCm38)	L82P	probably damaging	Het
Lrp2	A	T	2: 69,535,988 (GRCm38)	C256S	probably damaging	Het
Map1b	A	T	13: 99,432,427 (GRCm38)	I1262K	unknown	Het
Mctp1	C	T	13: 76,827,775 (GRCm38)	P756S	probably damaging	Het
Mdn1	T	A	4: 32,707,459 (GRCm38)	W1583R	probably damaging	Het
Mis12	T	A	11: 71,025,647 (GRCm38)	Y169N	probably benign	Het
Mplkipl1	C	T	19: 61,175,926 (GRCm38)	G24R	unknown	Het
Msh5	G	A	17: 35,038,420 (GRCm38)	Q333*	probably null	Het
Necap2	A	T	4: 141,072,523 (GRCm38)		probably null	Het
Nfatc2	T	C	2: 168,571,072 (GRCm38)	D211G	probably damaging	Het
Nlrx1	A	G	9: 44,253,429 (GRCm38)	V906A	possibly damaging	Het
Or2ak7	G	A	11: 58,684,763 (GRCm38)	V297I	probably damaging	Het
Or4a2	T	A	2: 89,417,772 (GRCm38)	I214F	probably benign	Het
Or4l1	A	T	14: 49,929,058 (GRCm38)	L162*	probably null	Het
Or5b125-ps1	A	T	19: 13,079,256 (GRCm38)		noncoding transcript	Het
Or8g24	A	G	9: 39,078,259 (GRCm38)	F162S	probably benign	Het
Pate5	A	G	9: 35,839,436 (GRCm38)	V30A	probably damaging	Het
Pkhd1l1	T	A	15: 44,533,891 (GRCm38)	N2057K	possibly damaging	Het
Ppip5k2	T	C	1: 97,711,749 (GRCm38)	K1078R	possibly damaging	Het
Prdm11	A	G	2: 92,980,609 (GRCm38)	I215T	probably damaging	Het
Ptpn13	C	T	5: 103,588,046 (GRCm38)	P2137L	probably benign	Het
Rnf17	T	C	14: 56,522,391 (GRCm38)	V1551A	probably benign	Het
Ror2	C	T	13: 53,117,147 (GRCm38)	V391I	probably benign	Het
Rps6ka2	A	T	17: 7,292,789 (GRCm38)	D542V	probably damaging	Het
Sema4g	A	G	19: 44,996,571 (GRCm38)		probably null	Het
Serpinb6d	T	G	13: 33,666,383 (GRCm38)	S64R	probably benign	Het
Serpine1	C	T	5: 137,069,351 (GRCm38)	R156K	probably benign	Het
Setdb2	A	G	14: 59,402,303 (GRCm38)	I713T	possibly damaging	Het
Slamf7	A	G	1: 171,639,125 (GRCm38)	F171L	probably benign	Het
Slc15a5	A	G	6: 138,073,066 (GRCm38)	L117S	probably damaging	Het
Slc8a3	T	A	12: 81,314,699 (GRCm38)	T449S	probably benign	Het
Slc8a3	T	A	12: 81,315,986 (GRCm38)	T20S	probably damaging	Het
Smc2	G	A	4: 52,462,926 (GRCm38)	V639M	possibly damaging	Het
Sra1	A	T	18: 36,676,441 (GRCm38)	C223*	probably null	Het
Srgap1	A	T	10: 121,785,552 (GRCm38)	M1012K	probably benign	Het
Stk-ps2	A	T	1: 46,029,442 (GRCm38)		noncoding transcript	Het
Taar6	A	T	10: 23,985,208 (GRCm38)	S147T	probably benign	Het
Taf15	G	A	11: 83,484,811 (GRCm38)	G34D	possibly damaging	Het
Tarbp1	T	C	8: 126,447,445 (GRCm38)	E874G	possibly damaging	Het
Tob2	T	C	15: 81,851,723 (GRCm38)	Y15C	probably damaging	Het
Trim12a	A	G	7: 104,304,358 (GRCm38)	V182A	possibly damaging	Het
Trim67	A	G	8: 124,794,667 (GRCm38)	E256G	probably benign	Het
Trrap	T	A	5: 144,805,720 (GRCm38)	S1382T	possibly damaging	Het
Ttc39d	T	A	17: 80,216,033 (GRCm38)	S40R	probably benign	Het
Ttn	A	T	2: 76,949,062 (GRCm38)	V1158E	probably benign	Het
Vmn1r19	C	T	6: 57,404,942 (GRCm38)	T160I	probably benign	Het
Vmn2r100	T	C	17: 19,532,038 (GRCm38)	I781T	probably benign	Het
Vmn2r85	T	C	10: 130,425,244 (GRCm38)	E408G	probably damaging	Het
Vwde	T	C	6: 13,187,139 (GRCm38)	D783G	probably damaging	Het
Wdr27	A	T	17: 14,876,133 (GRCm38)	D796E	probably damaging	Het
Zfp365	C	T	10: 67,889,991 (GRCm38)		probably null	Het
Zfp9	T	G	6: 118,464,447 (GRCm38)	H418P	probably damaging	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2125:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2145:Rif1	UTSW	2	52,111,400 (GRCm38)	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52,098,471 (GRCm38)	nonsense	probably null
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52,111,824 (GRCm38)	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52,111,139 (GRCm38)	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCCACTATGGTTCTTTGCC -3'
(R):5'- AGCTGAGGTCCAAGTCTCATCAG -3'

Sequencing Primer
(F):5'- CTAAATGGGAGTAGCATCCCTTCAG -3'
(R):5'- GAGGTCCAAGTCTCATCAGTAAATAC -3'

Posted On

2016-04-27