Mutagenetix > Incidental Mutation

Incidental Mutation 'R4952:Mga'

382038

Institutional Source

Beutler Lab

Gene Symbol

Mga

Ensembl Gene

ENSMUSG00000033943

Gene Name

MAX gene associated

Synonyms

D030062C11Rik, Mga, Mad5, C130042M01Rik

MMRRC Submission

042549-MU

Accession Numbers

Ncbi RefSeq: NM_013720.2, NM_001164274.1; MGI: 1352483

Essential gene?

Probably essential (E-score: 0.955) question?

Stock #

R4952 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119897228-119969581 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 119903301 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 210 (E210G)

Ref Sequence

ENSEMBL: ENSMUSP00000119044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046717] [ENSMUST00000079934] [ENSMUST00000110773] [ENSMUST00000110774] [ENSMUST00000156510]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000046717
AA Change: E210G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000043795
Gene: ENSMUSG00000033943
AA Change: E210G

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	6e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1796	1818	N/A	INTRINSIC
low complexity region	1833	1850	N/A	INTRINSIC
low complexity region	1977	1992	N/A	INTRINSIC
low complexity region	2183	2197	N/A	INTRINSIC
low complexity region	2241	2259	N/A	INTRINSIC
HLH	2368	2419	8.27e-7	SMART
low complexity region	2748	2769	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000079934
AA Change: E210G

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000078853
Gene: ENSMUSG00000033943
AA Change: E210G

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART
low complexity region	2578	2599	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110773
AA Change: E210G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000106400
Gene: ENSMUSG00000033943
AA Change: E210G

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	890	899	N/A	INTRINSIC
low complexity region	938	952	N/A	INTRINSIC
low complexity region	1033	1059	N/A	INTRINSIC
low complexity region	1169	1190	N/A	INTRINSIC
low complexity region	1222	1236	N/A	INTRINSIC
low complexity region	1485	1502	N/A	INTRINSIC
low complexity region	1555	1570	N/A	INTRINSIC
low complexity region	1602	1637	N/A	INTRINSIC
low complexity region	1717	1739	N/A	INTRINSIC
low complexity region	1754	1771	N/A	INTRINSIC
low complexity region	1898	1913	N/A	INTRINSIC
low complexity region	2104	2118	N/A	INTRINSIC
low complexity region	2162	2180	N/A	INTRINSIC
HLH	2289	2340	8.27e-7	SMART
low complexity region	2669	2690	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110774
AA Change: E210G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000106401
Gene: ENSMUSG00000033943
AA Change: E210G

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	7e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
Pfam:DUF4801	1037	1085	1e-19	PFAM
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1835	1857	N/A	INTRINSIC
low complexity region	1872	1889	N/A	INTRINSIC
low complexity region	2016	2031	N/A	INTRINSIC
low complexity region	2222	2236	N/A	INTRINSIC
low complexity region	2280	2298	N/A	INTRINSIC
HLH	2407	2458	8.27e-7	SMART
low complexity region	2787	2808	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129405

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141776

Predicted Effect

probably damaging
Transcript: ENSMUST00000156510
AA Change: E210G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000119044
Gene: ENSMUSG00000033943
AA Change: E210G

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	4e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART

Meta Mutation Damage Score

0.0921

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.5%
20x: 93.0%

Validation Efficiency

99% (93/94)

MGI Phenotype

PHENOTYPE: Embryos homozygous for a gene trap allele die shortly after implantation due to defective development of the inner cell mass (ICM) and the epiblast. ICM derivatives fail to develop past E4.5 and show increased apoptosis but no change in cell proliferation. [provided by MGI curators]

Allele List at MGI

All alleles(135) : Gene trapped(135)

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	G	5: 109,739,197		probably null	Het
4933421I07Rik	T	C	7: 42,447,659	Y76C	possibly damaging	Het
Adcy4	C	T	14: 55,779,029	D322N	probably damaging	Het
Ak9	A	G	10: 41,420,589	M1444V	probably benign	Het
Amfr	A	G	8: 93,973,159		probably benign	Het
Ankef1	A	G	2: 136,550,529	E546G	probably damaging	Het
Ankrd24	A	G	10: 81,647,148	M977V	probably benign	Het
Ap3m1	A	T	14: 21,040,066	S5T	probably benign	Het
Aqr	C	A	2: 114,109,937	D1243Y	probably damaging	Het
Arhgef2	T	C	3: 88,642,462	L591P	probably damaging	Het
Arid4a	C	A	12: 71,023,525	T70K	possibly damaging	Het
Asphd1	C	T	7: 126,948,685	A149T	probably benign	Het
Avpr1a	T	A	10: 122,449,754	M317K	probably damaging	Het
Birc2	T	C	9: 7,836,740	I109V	probably damaging	Het
Catsperd	A	G	17: 56,632,303	Y44C	probably damaging	Het
Ccdc114	A	C	7: 45,942,191	E293A	probably damaging	Het
Crygb	T	G	1: 65,082,109	S20R	probably benign	Het
Cyp3a25	T	C	5: 145,991,524	N237S	probably benign	Het
Dkk3	C	T	7: 112,118,351	A304T	probably benign	Het
Dst	T	C	1: 34,271,422	L4101S	probably damaging	Het
Dysf	A	G	6: 84,149,986	N1407S	possibly damaging	Het
Epb41	A	G	4: 132,000,270	V265A	probably damaging	Het
Faim2	C	T	15: 99,521,228	E75K	possibly damaging	Het
Fbn1	T	A	2: 125,317,534	D2208V	probably damaging	Het
Fbxo28	A	G	1: 182,326,385	S129P	probably damaging	Het
Fbxw14	T	A	9: 109,276,201	I299L	probably benign	Het
Fras1	C	T	5: 96,647,498	A1050V	probably benign	Het
Fyb	C	A	15: 6,638,811	T495K	probably damaging	Het
Ghdc	A	T	11: 100,769,151	W257R	probably damaging	Het
Gm10719	T	C	9: 3,018,962	L69S	probably benign	Het
Gm11492	A	G	11: 87,567,772	N324S	probably benign	Het
Gm12250	G	T	11: 58,188,384		noncoding transcript	Het
Gm4846	A	T	1: 166,483,934	F452Y	probably damaging	Het
Gm8773	T	A	5: 5,575,387	F28I	probably benign	Het
Gm884	T	C	11: 103,614,207	T2312A	possibly damaging	Het
Gpbp1	T	C	13: 111,440,750	D202G	probably damaging	Het
Gpd2	C	A	2: 57,307,013	Y193*	probably null	Het
Grhl2	G	T	15: 37,287,249	R229L	probably benign	Het
Gtf2a1	A	G	12: 91,575,749	F59L	possibly damaging	Het
Heatr1	G	T	13: 12,410,599	W640L	probably benign	Het
Kalrn	A	T	16: 34,357,415		probably null	Het
Keap1	T	C	9: 21,237,286	T142A	probably damaging	Het
Kpna2	G	A	11: 106,991,235	T255M	probably damaging	Het
Kpna3	A	G	14: 61,370,389	C456R	probably damaging	Het
Lama1	G	A	17: 67,767,566		probably null	Het
Mag	C	A	7: 30,909,156	E178*	probably null	Het
Map3k13	A	G	16: 21,911,019	I467V	probably benign	Het
Msi2	C	T	11: 88,366,784		probably null	Het
Naa16	A	T	14: 79,345,085	D521E	probably damaging	Het
Nav2	C	T	7: 49,304,540		probably benign	Het
Nek10	T	A	14: 14,860,986	L513M	possibly damaging	Het
Nek5	T	C	8: 22,096,799	K332R	probably benign	Het
Nek5	T	A	8: 22,079,088	I573L	probably benign	Het
Ntn1	CCTTCTTCT	CCTTCT	11: 68,213,026		probably benign	Het
Olfr111	A	T	17: 37,530,750	T258S	possibly damaging	Het
Olfr263	G	A	13: 21,133,344	V190I	probably benign	Het
Olfr773	T	A	10: 129,186,597	T275S	probably benign	Het
Olfr875	T	A	9: 37,773,064	M135K	probably damaging	Het
Orai1	T	G	5: 123,029,250	V162G	probably damaging	Het
P2rx6	T	C	16: 17,567,444	S134P	probably damaging	Het
Pappa2	G	A	1: 158,857,136	T811I	probably null	Het
Pcdhga10	T	C	18: 37,747,160		probably benign	Het
Pex16	C	T	2: 92,379,060	R241*	probably null	Het
Plxnb1	T	C	9: 109,114,836	F1969L	probably damaging	Het
Postn	A	G	3: 54,390,315		probably benign	Het
Prdm15	A	T	16: 97,806,077	I752N	probably damaging	Het
Rasgef1c	A	T	11: 49,979,512	K468M	probably damaging	Het
Rbfox1	A	G	16: 7,277,088	S111G	probably benign	Het
Rbm28	T	C	6: 29,138,598	D405G	probably damaging	Het
Rell1	A	G	5: 63,939,667		probably benign	Het
Rfx3	A	G	19: 27,830,672	S224P	probably damaging	Het
Scarb2	A	T	5: 92,454,777	I260K	probably damaging	Het
Slc15a4	A	G	5: 127,603,837	F72L	probably damaging	Het
Spg7	C	A	8: 123,090,171	R534S	probably damaging	Het
Stoml2	T	C	4: 43,029,589	T164A	probably benign	Het
Syt11	C	T	3: 88,762,283	G101S	possibly damaging	Het
Traj12	A	G	14: 54,206,556		probably benign	Het
Traj7	A	T	14: 54,211,524		probably benign	Het
Tysnd1	C	T	10: 61,702,076	T175I	possibly damaging	Het
Usp48	T	G	4: 137,606,693	Y139*	probably null	Het
Vmn2r72	A	G	7: 85,751,109	L244P	probably benign	Het
Wasf1	C	A	10: 40,936,190	P325Q	unknown	Het
Zc3h18	T	A	8: 122,410,900		probably benign	Het
Zfp712	A	G	13: 67,040,841	S541P	possibly damaging	Het

Other mutations in Mga

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Mga	APN	2	119,919,814 (GRCm38)	missense	possibly damaging	0.65
IGL00719:Mga	APN	2	119,947,453 (GRCm38)	nonsense	probably null
IGL01619:Mga	APN	2	119,931,828 (GRCm38)	missense	possibly damaging	0.46
IGL01721:Mga	APN	2	119,935,239 (GRCm38)	missense	probably damaging	1.00
IGL01759:Mga	APN	2	119,951,195 (GRCm38)	missense	possibly damaging	0.92
IGL01785:Mga	APN	2	119,902,912 (GRCm38)	missense	probably damaging	1.00
IGL01786:Mga	APN	2	119,902,912 (GRCm38)	missense	probably damaging	1.00
IGL01950:Mga	APN	2	119,941,654 (GRCm38)	missense	possibly damaging	0.60
IGL01960:Mga	APN	2	119,938,657 (GRCm38)	missense	probably damaging	1.00
IGL02086:Mga	APN	2	119,924,036 (GRCm38)	missense	probably damaging	0.99
IGL02364:Mga	APN	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
IGL02602:Mga	APN	2	119,931,884 (GRCm38)	missense	possibly damaging	0.66
IGL02751:Mga	APN	2	119,947,770 (GRCm38)	missense	possibly damaging	0.82
IGL02794:Mga	APN	2	119,946,289 (GRCm38)	missense	possibly damaging	0.84
IGL03247:Mga	APN	2	119,935,513 (GRCm38)	missense	possibly damaging	0.81
IGL03303:Mga	APN	2	119,903,452 (GRCm38)	missense	probably damaging	1.00
PIT4515001:Mga	UTSW	2	119,916,504 (GRCm38)	missense	probably damaging	1.00
R0060:Mga	UTSW	2	119,960,961 (GRCm38)	critical splice donor site	probably null
R0060:Mga	UTSW	2	119,960,961 (GRCm38)	critical splice donor site	probably null
R0417:Mga	UTSW	2	119,902,790 (GRCm38)	missense	probably damaging	0.99
R0449:Mga	UTSW	2	119,941,381 (GRCm38)	missense	probably damaging	1.00
R0457:Mga	UTSW	2	119,916,488 (GRCm38)	missense	probably damaging	0.98
R0538:Mga	UTSW	2	119,919,706 (GRCm38)	critical splice donor site	probably null
R0568:Mga	UTSW	2	119,935,422 (GRCm38)	missense	probably damaging	1.00
R0614:Mga	UTSW	2	119,964,466 (GRCm38)	missense	probably damaging	1.00
R0671:Mga	UTSW	2	119,919,910 (GRCm38)	splice site	probably null
R0811:Mga	UTSW	2	119,947,961 (GRCm38)	missense	probably damaging	0.99
R0812:Mga	UTSW	2	119,947,961 (GRCm38)	missense	probably damaging	0.99
R0948:Mga	UTSW	2	119,941,659 (GRCm38)	missense	possibly damaging	0.77
R1177:Mga	UTSW	2	119,926,446 (GRCm38)	missense	probably damaging	1.00
R1445:Mga	UTSW	2	119,902,698 (GRCm38)	missense	probably damaging	1.00
R1476:Mga	UTSW	2	119,941,675 (GRCm38)	missense	probably damaging	0.96
R1527:Mga	UTSW	2	119,916,597 (GRCm38)	missense	probably damaging	1.00
R1583:Mga	UTSW	2	119,963,960 (GRCm38)	missense	possibly damaging	0.66
R1592:Mga	UTSW	2	119,964,666 (GRCm38)	missense	possibly damaging	0.93
R1627:Mga	UTSW	2	119,964,562 (GRCm38)	missense	probably damaging	1.00
R1658:Mga	UTSW	2	119,941,689 (GRCm38)	missense	possibly damaging	0.63
R1677:Mga	UTSW	2	119,960,852 (GRCm38)	missense	possibly damaging	0.92
R1887:Mga	UTSW	2	119,923,617 (GRCm38)	missense	probably damaging	1.00
R1908:Mga	UTSW	2	119,926,594 (GRCm38)	missense	possibly damaging	0.66
R1909:Mga	UTSW	2	119,926,594 (GRCm38)	missense	possibly damaging	0.66
R2061:Mga	UTSW	2	119,964,980 (GRCm38)	unclassified	probably benign
R2145:Mga	UTSW	2	119,964,157 (GRCm38)	missense	possibly damaging	0.85
R2159:Mga	UTSW	2	119,919,643 (GRCm38)	missense	probably damaging	0.96
R2179:Mga	UTSW	2	119,960,442 (GRCm38)	missense	probably damaging	0.99
R2281:Mga	UTSW	2	119,903,723 (GRCm38)	missense	probably benign
R2423:Mga	UTSW	2	119,964,793 (GRCm38)	missense	probably damaging	1.00
R3620:Mga	UTSW	2	119,916,668 (GRCm38)	missense	probably damaging	1.00
R3622:Mga	UTSW	2	119,941,764 (GRCm38)	missense	probably damaging	1.00
R3624:Mga	UTSW	2	119,941,764 (GRCm38)	missense	probably damaging	1.00
R3802:Mga	UTSW	2	119,947,339 (GRCm38)	missense	probably damaging	0.96
R4011:Mga	UTSW	2	119,931,780 (GRCm38)	missense	probably damaging	1.00
R4065:Mga	UTSW	2	119,947,002 (GRCm38)	missense	probably damaging	1.00
R4520:Mga	UTSW	2	119,948,098 (GRCm38)	missense	possibly damaging	0.85
R4649:Mga	UTSW	2	119,941,493 (GRCm38)	missense	possibly damaging	0.81
R4660:Mga	UTSW	2	119,938,623 (GRCm38)	intron	probably benign
R4757:Mga	UTSW	2	119,903,639 (GRCm38)	missense	possibly damaging	0.82
R4771:Mga	UTSW	2	119,964,294 (GRCm38)	missense	probably damaging	1.00
R4784:Mga	UTSW	2	119,903,057 (GRCm38)	missense	probably damaging	1.00
R4866:Mga	UTSW	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
R4900:Mga	UTSW	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
R4995:Mga	UTSW	2	119,932,582 (GRCm38)	nonsense	probably null
R5020:Mga	UTSW	2	119,951,173 (GRCm38)	nonsense	probably null
R5082:Mga	UTSW	2	119,903,344 (GRCm38)	missense	probably damaging	0.98
R5208:Mga	UTSW	2	119,947,981 (GRCm38)	missense	possibly damaging	0.83
R5454:Mga	UTSW	2	119,903,329 (GRCm38)	missense	probably damaging	0.99
R5466:Mga	UTSW	2	119,902,697 (GRCm38)	missense	probably damaging	1.00
R5484:Mga	UTSW	2	119,916,626 (GRCm38)	missense	possibly damaging	0.58
R5669:Mga	UTSW	2	119,903,426 (GRCm38)	missense	probably damaging	1.00
R5819:Mga	UTSW	2	119,941,263 (GRCm38)	missense	possibly damaging	0.61
R5916:Mga	UTSW	2	119,964,312 (GRCm38)	missense	probably benign	0.27
R5942:Mga	UTSW	2	119,946,959 (GRCm38)	missense	probably benign	0.41
R6305:Mga	UTSW	2	119,947,698 (GRCm38)	missense	probably benign	0.00
R6434:Mga	UTSW	2	119,923,938 (GRCm38)	missense	probably damaging	0.99
R6467:Mga	UTSW	2	119,946,295 (GRCm38)	missense	probably damaging	1.00
R6488:Mga	UTSW	2	119,960,907 (GRCm38)	missense	probably damaging	1.00
R6630:Mga	UTSW	2	119,923,659 (GRCm38)	missense	probably damaging	0.99
R6790:Mga	UTSW	2	119,923,754 (GRCm38)	missense	probably damaging	0.99
R7029:Mga	UTSW	2	119,923,550 (GRCm38)	missense	probably damaging	1.00
R7039:Mga	UTSW	2	119,932,678 (GRCm38)	missense	probably benign	0.28
R7088:Mga	UTSW	2	119,961,936 (GRCm38)	missense	probably damaging	1.00
R7195:Mga	UTSW	2	119,917,328 (GRCm38)	missense	probably damaging	1.00
R7273:Mga	UTSW	2	119,935,214 (GRCm38)	missense	probably damaging	1.00
R7286:Mga	UTSW	2	119,964,788 (GRCm38)	missense	possibly damaging	0.93
R7346:Mga	UTSW	2	119,935,527 (GRCm38)	missense	possibly damaging	0.56
R7383:Mga	UTSW	2	119,960,340 (GRCm38)	missense	probably damaging	0.99
R7469:Mga	UTSW	2	119,903,046 (GRCm38)	missense	probably damaging	1.00
R7484:Mga	UTSW	2	119,946,229 (GRCm38)	missense	probably damaging	0.99
R7537:Mga	UTSW	2	119,935,551 (GRCm38)	missense	probably damaging	0.97
R7781:Mga	UTSW	2	119,917,357 (GRCm38)	missense	probably damaging	1.00
R7921:Mga	UTSW	2	119,919,678 (GRCm38)	missense	probably damaging	1.00
R8165:Mga	UTSW	2	119,947,238 (GRCm38)	missense	probably benign	0.12
R8226:Mga	UTSW	2	119,960,385 (GRCm38)	missense	probably benign	0.33
R8305:Mga	UTSW	2	119,946,319 (GRCm38)	missense	possibly damaging	0.77
R8309:Mga	UTSW	2	119,960,930 (GRCm38)	missense	probably damaging	1.00
R8363:Mga	UTSW	2	119,963,926 (GRCm38)	missense	probably benign	0.43
R8388:Mga	UTSW	2	119,964,081 (GRCm38)	missense	probably benign	0.00
R8524:Mga	UTSW	2	119,941,516 (GRCm38)	missense	probably damaging	0.97
R8693:Mga	UTSW	2	119,963,926 (GRCm38)	missense	possibly damaging	0.65
R8837:Mga	UTSW	2	119,938,791 (GRCm38)	splice site	probably benign
R8916:Mga	UTSW	2	119,958,338 (GRCm38)	missense	possibly damaging	0.92
R8936:Mga	UTSW	2	119,964,228 (GRCm38)	missense	probably damaging	1.00
R9028:Mga	UTSW	2	119,947,589 (GRCm38)	missense	probably benign
R9145:Mga	UTSW	2	119,964,012 (GRCm38)	missense	probably benign
R9155:Mga	UTSW	2	119,926,532 (GRCm38)	missense	probably damaging	1.00
R9308:Mga	UTSW	2	119,923,888 (GRCm38)	missense	possibly damaging	0.91
R9342:Mga	UTSW	2	119,948,175 (GRCm38)	missense	probably benign
R9347:Mga	UTSW	2	119,903,037 (GRCm38)	missense	probably damaging	1.00
R9390:Mga	UTSW	2	119,963,851 (GRCm38)	missense	probably damaging	0.99
R9408:Mga	UTSW	2	119,935,518 (GRCm38)	missense	possibly damaging	0.92
R9488:Mga	UTSW	2	119,964,823 (GRCm38)	missense	possibly damaging	0.90
R9495:Mga	UTSW	2	119,951,195 (GRCm38)	missense	possibly damaging	0.92
R9521:Mga	UTSW	2	119,964,498 (GRCm38)	missense	probably damaging	0.99
R9780:Mga	UTSW	2	119,916,772 (GRCm38)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- ATCGGTATAAGTGGAATGGTCG -3'
(R):5'- CTGAGGCTTACTGCTCAACC -3'

Sequencing Primer
(F):5'- GAAGGCTGAACCCCATATTTTGG -3'
(R):5'- CCATCATCCCGAAAGCCTTTGG -3'

Posted On

2016-04-27