|Institutional Source||Beutler Lab|
|Gene Name||dickkopf WNT signaling pathway inhibitor 3|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4952 (G1)|
|Chromosomal Location||112116017-112159057 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 112118351 bp|
|Amino Acid Change||Alanine to Threonine at position 304 (A304T)|
|Ref Sequence||ENSEMBL: ENSMUSP00000033036 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033036]|
|Predicted Effect||probably benign
AA Change: A304T
PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
AA Change: A304T
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||99% (93/94)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are viable, fertile and euthyroid but exhibit hyperactivity, a slight but significant decrease in the frequency of natural killer cells, and significantly increased IgM, hemoglobin, and hematocrit levels. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dkk3||
(F):5'- CTTGTCAAGCCAGAGGAAAAGC -3'
(R):5'- CCCGAGGTTTCACATGTAGTTG -3'
(F):5'- TCCACACTTGGGGACACTC -3'
(R):5'- GTAGTTGACATGCTCCAAGCCTG -3'