Mutagenetix > Incidental Mutations

Incidental Mutation 'R4952:Olfr875'

382068

Institutional Source

Beutler Lab

Gene Symbol

Olfr875

Ensembl Gene

ENSMUSG00000058628

Gene Name

olfactory receptor 875

Synonyms

GA_x6K02T2PVTD-31458511-31459443, MOR161-4

MMRRC Submission

042549-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R4952 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

37771857-37774459 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 37773064 bp

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 135 (M135K)

Ref Sequence

ENSEMBL: ENSMUSP00000150684 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080634] [ENSMUST00000215128]

Predicted Effect

probably damaging
Transcript: ENSMUST00000080634
AA Change: M135K

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000079467
Gene: ENSMUSG00000058628
AA Change: M135K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	1.1e-48	PFAM
Pfam:7tm_1	40	289	1.8e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215128
AA Change: M135K

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

Meta Mutation Damage Score

0.5398

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.5%
20x: 93.0%

Validation Efficiency

99% (93/94)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	G	5: 109,739,197		probably null	Het
4933421I07Rik	T	C	7: 42,447,659	Y76C	possibly damaging	Het
Adcy4	C	T	14: 55,779,029	D322N	probably damaging	Het
Ak9	A	G	10: 41,420,589	M1444V	probably benign	Het
Amfr	A	G	8: 93,973,159		probably benign	Het
Ankef1	A	G	2: 136,550,529	E546G	probably damaging	Het
Ankrd24	A	G	10: 81,647,148	M977V	probably benign	Het
Ap3m1	A	T	14: 21,040,066	S5T	probably benign	Het
Aqr	C	A	2: 114,109,937	D1243Y	probably damaging	Het
Arhgef2	T	C	3: 88,642,462	L591P	probably damaging	Het
Arid4a	C	A	12: 71,023,525	T70K	possibly damaging	Het
Asphd1	C	T	7: 126,948,685	A149T	probably benign	Het
Avpr1a	T	A	10: 122,449,754	M317K	probably damaging	Het
Birc2	T	C	9: 7,836,740	I109V	probably damaging	Het
Catsperd	A	G	17: 56,632,303	Y44C	probably damaging	Het
Ccdc114	A	C	7: 45,942,191	E293A	probably damaging	Het
Crygb	T	G	1: 65,082,109	S20R	probably benign	Het
Cyp3a25	T	C	5: 145,991,524	N237S	probably benign	Het
Dkk3	C	T	7: 112,118,351	A304T	probably benign	Het
Dst	T	C	1: 34,271,422	L4101S	probably damaging	Het
Dysf	A	G	6: 84,149,986	N1407S	possibly damaging	Het
Epb41	A	G	4: 132,000,270	V265A	probably damaging	Het
Faim2	C	T	15: 99,521,228	E75K	possibly damaging	Het
Fbn1	T	A	2: 125,317,534	D2208V	probably damaging	Het
Fbxo28	A	G	1: 182,326,385	S129P	probably damaging	Het
Fbxw14	T	A	9: 109,276,201	I299L	probably benign	Het
Fras1	C	T	5: 96,647,498	A1050V	probably benign	Het
Fyb	C	A	15: 6,638,811	T495K	probably damaging	Het
Ghdc	A	T	11: 100,769,151	W257R	probably damaging	Het
Gm10719	T	C	9: 3,018,962	L69S	probably benign	Het
Gm11492	A	G	11: 87,567,772	N324S	probably benign	Het
Gm12250	G	T	11: 58,188,384		noncoding transcript	Het
Gm4846	A	T	1: 166,483,934	F452Y	probably damaging	Het
Gm8773	T	A	5: 5,575,387	F28I	probably benign	Het
Gm884	T	C	11: 103,614,207	T2312A	possibly damaging	Het
Gpbp1	T	C	13: 111,440,750	D202G	probably damaging	Het
Gpd2	C	A	2: 57,307,013	Y193*	probably null	Het
Grhl2	G	T	15: 37,287,249	R229L	probably benign	Het
Gtf2a1	A	G	12: 91,575,749	F59L	possibly damaging	Het
Heatr1	G	T	13: 12,410,599	W640L	probably benign	Het
Kalrn	A	T	16: 34,357,415		probably null	Het
Keap1	T	C	9: 21,237,286	T142A	probably damaging	Het
Kpna2	G	A	11: 106,991,235	T255M	probably damaging	Het
Kpna3	A	G	14: 61,370,389	C456R	probably damaging	Het
Lama1	G	A	17: 67,767,566		probably null	Het
Mag	C	A	7: 30,909,156	E178*	probably null	Het
Map3k13	A	G	16: 21,911,019	I467V	probably benign	Het
Mga	A	G	2: 119,903,301	E210G	probably damaging	Het
Msi2	C	T	11: 88,366,784		probably null	Het
Naa16	A	T	14: 79,345,085	D521E	probably damaging	Het
Nav2	C	T	7: 49,304,540		probably benign	Het
Nek10	T	A	14: 14,860,986	L513M	possibly damaging	Het
Nek5	T	C	8: 22,096,799	K332R	probably benign	Het
Nek5	T	A	8: 22,079,088	I573L	probably benign	Het
Ntn1	CCTTCTTCT	CCTTCT	11: 68,213,026		probably benign	Het
Olfr111	A	T	17: 37,530,750	T258S	possibly damaging	Het
Olfr263	G	A	13: 21,133,344	V190I	probably benign	Het
Olfr773	T	A	10: 129,186,597	T275S	probably benign	Het
Orai1	T	G	5: 123,029,250	V162G	probably damaging	Het
P2rx6	T	C	16: 17,567,444	S134P	probably damaging	Het
Pappa2	G	A	1: 158,857,136	T811I	probably null	Het
Pcdhga10	T	C	18: 37,747,160		probably benign	Het
Pex16	C	T	2: 92,379,060	R241*	probably null	Het
Plxnb1	T	C	9: 109,114,836	F1969L	probably damaging	Het
Postn	A	G	3: 54,390,315		probably benign	Het
Prdm15	A	T	16: 97,806,077	I752N	probably damaging	Het
Rasgef1c	A	T	11: 49,979,512	K468M	probably damaging	Het
Rbfox1	A	G	16: 7,277,088	S111G	probably benign	Het
Rbm28	T	C	6: 29,138,598	D405G	probably damaging	Het
Rell1	A	G	5: 63,939,667		probably benign	Het
Rfx3	A	G	19: 27,830,672	S224P	probably damaging	Het
Scarb2	A	T	5: 92,454,777	I260K	probably damaging	Het
Slc15a4	A	G	5: 127,603,837	F72L	probably damaging	Het
Spg7	C	A	8: 123,090,171	R534S	probably damaging	Het
Stoml2	T	C	4: 43,029,589	T164A	probably benign	Het
Syt11	C	T	3: 88,762,283	G101S	possibly damaging	Het
Traj12	A	G	14: 54,206,556		probably benign	Het
Traj7	A	T	14: 54,211,524		probably benign	Het
Tysnd1	C	T	10: 61,702,076	T175I	possibly damaging	Het
Usp48	T	G	4: 137,606,693	Y139*	probably null	Het
Vmn2r72	A	G	7: 85,751,109	L244P	probably benign	Het
Wasf1	C	A	10: 40,936,190	P325Q	unknown	Het
Zc3h18	T	A	8: 122,410,900		probably benign	Het
Zfp712	A	G	13: 67,040,841	S541P	possibly damaging	Het

Other mutations in Olfr875

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01909:Olfr875	APN	9	37772863	missense	possibly damaging	0.95
IGL02013:Olfr875	APN	9	37772889	missense	probably benign	0.00
IGL02162:Olfr875	APN	9	37772931	missense	probably benign	0.13
IGL02966:Olfr875	APN	9	37773586	missense	probably benign	0.28
R0017:Olfr875	UTSW	9	37772978	missense	probably benign	0.08
R0520:Olfr875	UTSW	9	37773553	missense	probably benign	0.00
R0553:Olfr875	UTSW	9	37773331	missense	probably benign	0.05
R0833:Olfr875	UTSW	9	37773076	missense	probably benign	0.03
R1316:Olfr875	UTSW	9	37772743	missense	possibly damaging	0.94
R1547:Olfr875	UTSW	9	37772664	missense	probably benign	0.00
R1888:Olfr875	UTSW	9	37772867	missense	possibly damaging	0.88
R1888:Olfr875	UTSW	9	37772867	missense	possibly damaging	0.88
R1891:Olfr875	UTSW	9	37772867	missense	possibly damaging	0.88
R1894:Olfr875	UTSW	9	37772867	missense	possibly damaging	0.88
R3055:Olfr875	UTSW	9	37773193	missense	probably damaging	1.00
R4816:Olfr875	UTSW	9	37773430	missense	possibly damaging	0.72
R4829:Olfr875	UTSW	9	37772947	missense	probably damaging	1.00
R6111:Olfr875	UTSW	9	37772932	missense	probably damaging	0.99
R6838:Olfr875	UTSW	9	37773052	missense	possibly damaging	0.87
R7101:Olfr875	UTSW	9	37772991	missense	probably damaging	0.99
R7104:Olfr875	UTSW	9	37773141	missense	possibly damaging	0.64
R7224:Olfr875	UTSW	9	37773415	missense	possibly damaging	0.95
R7334:Olfr875	UTSW	9	37772997	missense	probably damaging	0.97
R7582:Olfr875	UTSW	9	37772821	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCTGAACTCTCACCTTCATAC -3'
(R):5'- GTGTTGCTACAGGAGAGCTC -3'

Sequencing Primer
(F):5'- TTCCTCCACTATCATCCCAAAAATG -3'
(R):5'- TGTTGCTACAGGAGAGCTCAAGAAG -3'

Posted On

2016-04-27