Mutagenetix > Incidental Mutation

Incidental Mutation 'R4959:Fryl'

382141

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

9030227G01Rik, 2310004H21Rik, 2510002A14Rik

MMRRC Submission

042556-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.740) question?

Stock #

R4959 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73177534-73414042 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73192401 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 2678 (M2678K)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094700
AA Change: M2678K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: M2678K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000101127
AA Change: M2678K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: M2678K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146953

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156661

Predicted Effect

unknown
Transcript: ENSMUST00000202697
AA Change: M900K

Predicted Effect

probably benign
Transcript: ENSMUST00000202806

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.6%
20x: 89.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
6820408C15Rik	T	A	2: 152,282,808 (GRCm39)	I221K	possibly damaging	Het
Aadacl3	A	T	4: 144,183,656 (GRCm39)	C136S	probably benign	Het
Abhd16a	T	A	17: 35,321,318 (GRCm39)	S498T	probably benign	Het
Acrv1	G	A	9: 36,605,996 (GRCm39)		probably null	Het
Adamts18	G	A	8: 114,463,357 (GRCm39)	R830*	probably null	Het
Art3	A	G	5: 92,551,478 (GRCm39)	Y279C	probably damaging	Het
Atm	A	T	9: 53,426,601 (GRCm39)	D552E	probably benign	Het
Ccdc154	T	C	17: 25,389,888 (GRCm39)	L508P	probably damaging	Het
Ccser2	A	T	14: 36,662,753 (GRCm39)	S144T	probably benign	Het
Cgn	C	T	3: 94,685,564 (GRCm39)	A320T	probably benign	Het
Cklf	A	G	8: 104,988,184 (GRCm39)	K106E	probably benign	Het
Clec12a	C	A	6: 129,330,628 (GRCm39)	T70K	probably benign	Het
CN725425	G	A	15: 91,129,904 (GRCm39)	A256T	possibly damaging	Het
Crb2	G	T	2: 37,680,482 (GRCm39)	R470L	probably damaging	Het
Csn1s1	T	G	5: 87,821,120 (GRCm39)	S33A	probably benign	Het
D630045J12Rik	C	T	6: 38,125,302 (GRCm39)	V1571M	possibly damaging	Het
Deup1	G	A	9: 15,523,310 (GRCm39)	Q58*	probably null	Het
Dph2	T	A	4: 117,748,527 (GRCm39)	D82V	probably damaging	Het
Dsp	C	T	13: 38,375,686 (GRCm39)	A1157V	probably benign	Het
Evi5l	G	T	8: 4,255,406 (GRCm39)	V477L	probably benign	Het
Fam187b	T	A	7: 30,681,687 (GRCm39)	H131Q	probably damaging	Het
Fam193b	A	G	13: 55,691,097 (GRCm39)	I259T	probably damaging	Het
Fam91a1	A	G	15: 58,303,059 (GRCm39)	T323A	probably benign	Het
Fat4	T	G	3: 39,037,195 (GRCm39)	S3616A	probably benign	Het
Fbxo38	A	T	18: 62,655,578 (GRCm39)	N369K	probably benign	Het
Fitm1	T	A	14: 55,813,831 (GRCm39)		probably null	Het
Fkbp5	T	C	17: 28,647,343 (GRCm39)	E164G	probably damaging	Het
Fpr-rs3	T	C	17: 20,844,211 (GRCm39)	Y310C	possibly damaging	Het
Fsip2	T	C	2: 82,815,169 (GRCm39)	I3634T	probably benign	Het
Gm3233	T	C	10: 77,595,399 (GRCm39)		probably benign	Het
Gzmd	T	G	14: 56,369,955 (GRCm39)	T9P	unknown	Het
Htr2b	A	T	1: 86,027,813 (GRCm39)	I231N	probably damaging	Het
Il2ra	A	G	2: 11,681,664 (GRCm39)	T90A	possibly damaging	Het
Kbtbd2	A	G	6: 56,758,943 (GRCm39)	F60S	probably benign	Het
Kif21b	G	A	1: 136,076,108 (GRCm39)	C299Y	possibly damaging	Het
Lsm5	A	T	6: 56,680,309 (GRCm39)	D44E	probably damaging	Het
Mapk1	T	A	16: 16,836,170 (GRCm39)	M106K	probably damaging	Het
Mpp2	A	G	11: 101,954,117 (GRCm39)	V193A	probably damaging	Het
Muc4	T	C	16: 32,575,437 (GRCm39)	S1398P	possibly damaging	Het
Ndufs2	A	G	1: 171,065,578 (GRCm39)	Y282H	probably damaging	Het
Nes	T	A	3: 87,882,983 (GRCm39)	L414Q	probably damaging	Het
Neurl2	T	A	2: 164,675,122 (GRCm39)		probably null	Het
Ngef	A	G	1: 87,431,070 (GRCm39)	F159S	possibly damaging	Het
Nlrx1	T	C	9: 44,165,448 (GRCm39)	H816R	possibly damaging	Het
Or10v5	A	G	19: 11,806,300 (GRCm39)	L30P	probably benign	Het
Or1e22	A	T	11: 73,376,893 (GRCm39)	Y252*	probably null	Het
Or2f1	T	C	6: 42,721,621 (GRCm39)	S217P	probably damaging	Het
Or4c106	T	C	2: 88,682,516 (GRCm39)	V74A	possibly damaging	Het
Or4f17-ps1	T	A	2: 111,357,969 (GRCm39)	F103L	probably damaging	Het
Or4p7	T	A	2: 88,221,674 (GRCm39)	L28I	probably benign	Het
Or7g35	A	G	9: 19,496,504 (GRCm39)	T224A	probably benign	Het
Pcgf2	A	T	11: 97,582,515 (GRCm39)	Y7N	possibly damaging	Het
Pdlim5	G	A	3: 142,017,740 (GRCm39)		probably benign	Het
Pdzd2	A	G	15: 12,375,734 (GRCm39)	V1467A	probably damaging	Het
Pigp	C	A	16: 94,160,006 (GRCm39)	G134V	probably benign	Het
Pik3c2g	T	C	6: 139,789,657 (GRCm39)	Y385H	possibly damaging	Het
Pnliprp2	G	A	19: 58,754,750 (GRCm39)	E265K	probably benign	Het
Prl3c1	G	T	13: 27,386,471 (GRCm39)		probably null	Het
Ptpn4	A	T	1: 119,692,826 (GRCm39)	Y110*	probably null	Het
Ptprq	C	T	10: 107,522,416 (GRCm39)	V546I	probably damaging	Het
Rd3l	C	T	12: 111,946,578 (GRCm39)		probably null	Het
Rpl37	G	A	15: 5,147,128 (GRCm39)	R56Q	possibly damaging	Het
Rttn	A	T	18: 89,060,292 (GRCm39)	H998L	probably damaging	Het
Rufy1	A	T	11: 50,292,315 (GRCm39)	F491L	probably benign	Het
Simc1	T	G	13: 54,673,131 (GRCm39)	V493G	possibly damaging	Het
Slc22a14	CTTTCCTGAA	C	9: 119,003,101 (GRCm39)		probably benign	Het
Slc25a46	A	T	18: 31,735,807 (GRCm39)	I144N	possibly damaging	Het
Smtn	A	T	11: 3,477,825 (GRCm39)	M1K	probably null	Het
Snd1	C	A	6: 28,884,250 (GRCm39)	Y766*	probably null	Het
Spata31d1d	C	T	13: 59,875,102 (GRCm39)	G811D	probably damaging	Het
Spta1	A	C	1: 174,074,174 (GRCm39)	D2371A	probably damaging	Het
Stard6	A	C	18: 70,631,631 (GRCm39)	D74A	possibly damaging	Het
Stim2	G	T	5: 54,262,712 (GRCm39)	R318L	probably benign	Het
Sufu	C	T	19: 46,463,991 (GRCm39)	T401I	possibly damaging	Het
Suz12	G	A	11: 79,920,057 (GRCm39)	G539D	probably damaging	Het
Sytl2	C	G	7: 90,025,245 (GRCm39)	S411C	probably damaging	Het
Taf6	G	A	5: 138,181,465 (GRCm39)	Q156*	probably null	Het
Tead4	T	C	6: 128,247,950 (GRCm39)	D29G	probably damaging	Het
Thada	T	C	17: 84,751,611 (GRCm39)	Y455C	probably damaging	Het
Tlr6	G	C	5: 65,111,002 (GRCm39)	T635S	possibly damaging	Het
Tlr9	A	G	9: 106,101,876 (GRCm39)	D389G	probably benign	Het
Tmem54	A	T	4: 129,002,073 (GRCm39)	H40L	probably damaging	Het
Trappc9	G	T	15: 72,808,905 (GRCm39)	N540K	probably damaging	Het
Trbv28	C	T	6: 41,248,788 (GRCm39)	S106F	probably damaging	Het
Trrap	A	T	5: 144,793,770 (GRCm39)	H3384L	probably damaging	Het
Usp16	T	A	16: 87,277,802 (GRCm39)	M684K	probably damaging	Het
Vat1	G	A	11: 101,351,426 (GRCm39)	T305M	probably damaging	Het
Vmn2r85	A	G	10: 130,257,302 (GRCm39)	S544P	probably damaging	Het
Vps8	T	C	16: 21,278,536 (GRCm39)	S267P	probably damaging	Het
Ylpm1	T	A	12: 85,096,719 (GRCm39)	V1242E	probably damaging	Het
Zfp267	C	T	3: 36,218,285 (GRCm39)	R102C	possibly damaging	Het
Zfp322a	T	C	13: 23,541,052 (GRCm39)	Y230C	probably damaging	Het
Zfp352	T	A	4: 90,112,376 (GRCm39)	V172E	probably benign	Het
Zfp936	A	T	7: 42,839,034 (GRCm39)	H167L	probably damaging	Het
Zkscan14	A	G	5: 145,132,302 (GRCm39)	Y410H	probably benign	Het
Zkscan17	A	G	11: 59,394,538 (GRCm39)	M21T	probably damaging	Het
Zswim4	G	T	8: 84,938,852 (GRCm39)	A1010D	probably benign	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,305,451 (GRCm39)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,244,305 (GRCm39)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,211,940 (GRCm39)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,179,844 (GRCm39)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,279,707 (GRCm39)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,190,134 (GRCm39)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,282,112 (GRCm39)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,233,302 (GRCm39)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,267,519 (GRCm39)	splice site	probably benign
IGL02431:Fryl	APN	5	73,255,651 (GRCm39)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,222,636 (GRCm39)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,255,736 (GRCm39)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,227,220 (GRCm39)	intron	probably benign
IGL02642:Fryl	APN	5	73,252,809 (GRCm39)	missense	probably benign
IGL02657:Fryl	APN	5	73,212,203 (GRCm39)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,250,506 (GRCm39)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,216,726 (GRCm39)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,258,798 (GRCm39)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03183:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03275:Fryl	APN	5	73,305,376 (GRCm39)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,293,659 (GRCm39)	splice site	probably benign
IGL03341:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03343:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03350:Fryl	APN	5	73,290,649 (GRCm39)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,211,402 (GRCm39)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,267,624 (GRCm39)	splice site	probably benign
IGL03375:Fryl	APN	5	73,245,792 (GRCm39)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
Besotted	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,198,947 (GRCm39)	splice site	probably benign
R0312:Fryl	UTSW	5	73,230,231 (GRCm39)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,255,757 (GRCm39)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,244,315 (GRCm39)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,254,760 (GRCm39)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,221,840 (GRCm39)	splice site	probably benign
R0567:Fryl	UTSW	5	73,222,734 (GRCm39)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,282,077 (GRCm39)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,226,074 (GRCm39)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,222,702 (GRCm39)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,228,469 (GRCm39)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,246,539 (GRCm39)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,198,675 (GRCm39)	splice site	probably benign
R1076:Fryl	UTSW	5	73,282,016 (GRCm39)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,267,614 (GRCm39)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,222,268 (GRCm39)	splice site	probably benign
R1394:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,232,094 (GRCm39)	nonsense	probably null
R1664:Fryl	UTSW	5	73,216,778 (GRCm39)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,190,204 (GRCm39)	splice site	probably benign
R1937:Fryl	UTSW	5	73,290,710 (GRCm39)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,179,465 (GRCm39)	nonsense	probably null
R2036:Fryl	UTSW	5	73,265,305 (GRCm39)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,179,887 (GRCm39)	missense	probably benign
R2088:Fryl	UTSW	5	73,222,804 (GRCm39)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,279,642 (GRCm39)	missense	probably benign
R2106:Fryl	UTSW	5	73,255,674 (GRCm39)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,222,318 (GRCm39)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,265,890 (GRCm39)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,207,799 (GRCm39)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,240,193 (GRCm39)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,265,417 (GRCm39)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3787:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3837:Fryl	UTSW	5	73,228,608 (GRCm39)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,269,766 (GRCm39)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,243,903 (GRCm39)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,245,740 (GRCm39)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,238,396 (GRCm39)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,248,022 (GRCm39)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,257,636 (GRCm39)	missense	probably benign
R4700:Fryl	UTSW	5	73,222,881 (GRCm39)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,238,315 (GRCm39)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,198,705 (GRCm39)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,226,125 (GRCm39)	frame shift	probably null
R4948:Fryl	UTSW	5	73,246,473 (GRCm39)	missense	probably benign	0.08
R5062:Fryl	UTSW	5	73,233,236 (GRCm39)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,215,098 (GRCm39)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,248,061 (GRCm39)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,259,016 (GRCm39)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,243,943 (GRCm39)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,222,479 (GRCm39)	nonsense	probably null
R5275:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,262,117 (GRCm39)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,231,247 (GRCm39)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,257,808 (GRCm39)	missense	probably benign
R5778:Fryl	UTSW	5	73,230,121 (GRCm39)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,248,098 (GRCm39)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,248,060 (GRCm39)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,254,715 (GRCm39)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,240,638 (GRCm39)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,257,340 (GRCm39)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,275,894 (GRCm39)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,270,131 (GRCm39)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,222,824 (GRCm39)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,349,102 (GRCm39)	intron	probably benign
R6310:Fryl	UTSW	5	73,349,104 (GRCm39)	intron	probably benign
R6429:Fryl	UTSW	5	73,248,094 (GRCm39)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,216,859 (GRCm39)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,290,655 (GRCm39)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,289,824 (GRCm39)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,212,124 (GRCm39)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,179,575 (GRCm39)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,222,437 (GRCm39)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,222,560 (GRCm39)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,222,375 (GRCm39)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,226,146 (GRCm39)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,179,485 (GRCm39)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,179,554 (GRCm39)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,231,272 (GRCm39)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,207,773 (GRCm39)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,212,951 (GRCm39)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,248,099 (GRCm39)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,231,251 (GRCm39)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,279,653 (GRCm39)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,222,438 (GRCm39)	missense	probably benign
R7236:Fryl	UTSW	5	73,265,821 (GRCm39)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,204,839 (GRCm39)	splice site	probably null
R7425:Fryl	UTSW	5	73,262,091 (GRCm39)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,181,331 (GRCm39)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,254,904 (GRCm39)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,255,539 (GRCm39)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,180,019 (GRCm39)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,238,382 (GRCm39)	missense	probably benign
R7548:Fryl	UTSW	5	73,349,105 (GRCm39)	missense	unknown
R7565:Fryl	UTSW	5	73,191,063 (GRCm39)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,245,739 (GRCm39)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,179,843 (GRCm39)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,267,588 (GRCm39)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,240,727 (GRCm39)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,228,641 (GRCm39)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,211,875 (GRCm39)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,259,150 (GRCm39)	splice site	probably null
R8110:Fryl	UTSW	5	73,290,620 (GRCm39)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,228,527 (GRCm39)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,207,682 (GRCm39)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,257,843 (GRCm39)	splice site	probably null
R8263:Fryl	UTSW	5	73,238,348 (GRCm39)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8359:Fryl	UTSW	5	73,233,276 (GRCm39)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,293,663 (GRCm39)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,275,790 (GRCm39)	makesense	probably null
R8450:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8514:Fryl	UTSW	5	73,242,699 (GRCm39)	missense	probably benign
R8536:Fryl	UTSW	5	73,257,696 (GRCm39)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,247,997 (GRCm39)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,289,905 (GRCm39)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,226,185 (GRCm39)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,182,118 (GRCm39)	missense
R9063:Fryl	UTSW	5	73,238,346 (GRCm39)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,265,920 (GRCm39)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,348,862 (GRCm39)	intron	probably benign
R9345:Fryl	UTSW	5	73,207,754 (GRCm39)	missense	probably benign
R9381:Fryl	UTSW	5	73,240,637 (GRCm39)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,349,152 (GRCm39)	missense	unknown
R9401:Fryl	UTSW	5	73,222,563 (GRCm39)	nonsense	probably null
R9497:Fryl	UTSW	5	73,215,134 (GRCm39)	missense
R9514:Fryl	UTSW	5	73,262,115 (GRCm39)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,179,498 (GRCm39)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,275,801 (GRCm39)	missense	probably benign
R9665:Fryl	UTSW	5	73,222,299 (GRCm39)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,216,879 (GRCm39)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,192,402 (GRCm39)	missense	probably benign
Z1088:Fryl	UTSW	5	73,248,081 (GRCm39)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,248,052 (GRCm39)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,230,180 (GRCm39)	missense	probably benign
Z1177:Fryl	UTSW	5	73,198,938 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CAAGCATGCTTCTTAAAGGGAATCTG -3'
(R):5'- ATTCAGTGGCCTAAAGTCCTAG -3'

Sequencing Primer
(F):5'- TCTGCAGGGATACTAAAGCCTG -3'
(R):5'- TGGCCTAAAGTCCTAGGAGTC -3'

Posted On

2016-04-27