Mutagenetix > Incidental Mutation

Incidental Mutation 'R4973:Zfp352'

382357

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

042568-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4973 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

90218820-90225702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 90224139 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 172 (V172E)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

probably benign
Transcript: ENSMUST00000080541
AA Change: V172E

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: V172E

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107129
AA Change: V172E

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: V172E

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.9%

Validation Efficiency

97% (115/118)

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6820408C15Rik	T	A	2: 152,440,888 (GRCm38)	I221K	possibly damaging	Het
Abhd16a	T	A	17: 35,102,342 (GRCm38)	S498T	probably benign	Het
Adamts18	G	A	8: 113,736,725 (GRCm38)	R830*	probably null	Het
Ankrd27	A	G	7: 35,632,992 (GRCm38)	D848G	probably benign	Het
Art3	A	G	5: 92,403,619 (GRCm38)	Y279C	probably damaging	Het
Atp2c2	A	G	8: 119,754,263 (GRCm38)	T797A	probably benign	Het
Ccdc121	A	T	1: 181,511,264 (GRCm38)	V41E	possibly damaging	Het
Ccdc122	A	T	14: 77,067,941 (GRCm38)	I12F	possibly damaging	Het
Ccdc154	T	C	17: 25,170,914 (GRCm38)	L508P	probably damaging	Het
Cdk5r2	A	G	1: 74,855,672 (GRCm38)	D192G	probably damaging	Het
Cgn	C	T	3: 94,778,254 (GRCm38)	A320T	probably benign	Het
Cklf	A	G	8: 104,261,552 (GRCm38)	K106E	probably benign	Het
Clec12a	C	A	6: 129,353,665 (GRCm38)	T70K	probably benign	Het
Clock	A	G	5: 76,254,411 (GRCm38)	V134A	possibly damaging	Het
CN725425	G	A	15: 91,245,701 (GRCm38)	A256T	possibly damaging	Het
Csf1r	A	T	18: 61,129,047 (GRCm38)	I792F	probably damaging	Het
Csn1s1	T	G	5: 87,673,261 (GRCm38)	S33A	probably benign	Het
Cspp1	T	A	1: 10,126,463 (GRCm38)	N900K	probably damaging	Het
D3Ertd254e	C	T	3: 36,164,136 (GRCm38)	R102C	possibly damaging	Het
D5Ertd579e	A	G	5: 36,672,905 (GRCm38)	V25A	probably benign	Het
D630045J12Rik	C	T	6: 38,148,367 (GRCm38)	V1571M	possibly damaging	Het
Ddx5	C	A	11: 106,785,007 (GRCm38)	V286L	possibly damaging	Het
Deup1	G	A	9: 15,612,014 (GRCm38)	Q58*	probably null	Het
Dnhd1	T	G	7: 105,713,633 (GRCm38)	L3801V	probably benign	Het
Dph2	T	A	4: 117,891,330 (GRCm38)	D82V	probably damaging	Het
Ercc6	A	G	14: 32,574,902 (GRCm38)	D1283G	probably damaging	Het
Evi5l	G	T	8: 4,205,406 (GRCm38)	V477L	probably benign	Het
Fam91a1	A	G	15: 58,431,210 (GRCm38)	T323A	probably benign	Het
Fanca	G	A	8: 123,308,522 (GRCm38)	T228M	probably damaging	Het
Fat4	T	G	3: 38,983,046 (GRCm38)	S3616A	probably benign	Het
Fbxo16	G	T	14: 65,321,297 (GRCm38)	A302S	probably benign	Het
Fkbp5	T	C	17: 28,428,369 (GRCm38)	E164G	probably damaging	Het
Fpr-rs3	T	C	17: 20,623,949 (GRCm38)	Y310C	possibly damaging	Het
Fsip2	T	C	2: 82,984,825 (GRCm38)	I3634T	probably benign	Het
Gm6327	T	C	16: 12,760,981 (GRCm38)		noncoding transcript	Het
Gnl3	A	T	14: 31,013,505 (GRCm38)	N411K	possibly damaging	Het
Golga1	A	T	2: 39,039,106 (GRCm38)	D308E	probably damaging	Het
Gpbar1	TACCAC	TAC	1: 74,279,545 (GRCm38)		probably benign	Het
Gsap	A	T	5: 21,254,039 (GRCm38)	T501S	probably benign	Het
Helq	A	G	5: 100,792,871 (GRCm38)		probably benign	Het
Hmcn2	A	G	2: 31,344,096 (GRCm38)	H291R	probably benign	Het
Htt	A	T	5: 34,813,023 (GRCm38)	D505V	probably damaging	Het
Ildr1	C	T	16: 36,708,298 (GRCm38)	T35I	probably benign	Het
Iqgap2	A	G	13: 95,657,797 (GRCm38)		probably null	Het
Kansl1	C	T	11: 104,424,321 (GRCm38)	R297H	probably damaging	Het
Kat2b-ps	T	C	5: 93,391,785 (GRCm38)		noncoding transcript	Het
Kbtbd2	A	G	6: 56,781,958 (GRCm38)	F60S	probably benign	Het
Kcnt2	A	T	1: 140,609,650 (GRCm38)	S1116C	probably damaging	Het
Krt9	C	T	11: 100,188,712 (GRCm38)	G618E	unknown	Het
Lsm5	A	T	6: 56,703,324 (GRCm38)	D44E	probably damaging	Het
Mdn1	A	G	4: 32,734,418 (GRCm38)	D3275G	probably benign	Het
Mindy2	A	T	9: 70,605,171 (GRCm38)	V599E	possibly damaging	Het
Nelfa	A	G	5: 33,901,818 (GRCm38)	V231A	probably benign	Het
Nepro	T	C	16: 44,734,793 (GRCm38)	Y411H	probably benign	Het
Nes	T	A	3: 87,975,676 (GRCm38)	L414Q	probably damaging	Het
Neurl2	T	A	2: 164,833,202 (GRCm38)		probably null	Het
Nkx1-1	G	T	5: 33,431,066 (GRCm38)	Q293K	possibly damaging	Het
Nphp3	C	T	9: 104,031,999 (GRCm38)	H803Y	probably benign	Het
Obscn	A	G	11: 59,132,466 (GRCm38)	V695A	probably damaging	Het
Olfr105-ps	G	A	17: 37,383,019 (GRCm38)	A151T	probably damaging	Het
Olfr1178	T	A	2: 88,391,330 (GRCm38)	L28I	probably benign	Het
Olfr1204	T	C	2: 88,852,172 (GRCm38)	V74A	possibly damaging	Het
Olfr1293-ps	T	A	2: 111,527,624 (GRCm38)	F103L	probably damaging	Het
Olfr1417	A	G	19: 11,828,936 (GRCm38)	L30P	probably benign	Het
Olfr330	T	C	11: 58,529,077 (GRCm38)	E303G	probably benign	Het
Olfr453	T	C	6: 42,744,687 (GRCm38)	S217P	probably damaging	Het
Olfr855	A	G	9: 19,585,208 (GRCm38)	T224A	probably benign	Het
Olfr926	A	G	9: 38,878,104 (GRCm38)	*309W	probably null	Het
Orai3	T	A	7: 127,774,176 (GRCm38)	L283Q	probably damaging	Het
Pcdhb13	A	T	18: 37,443,184 (GRCm38)	D205V	probably benign	Het
Pdlim5	G	A	3: 142,311,979 (GRCm38)		probably benign	Het
Pdzd2	A	G	15: 12,375,648 (GRCm38)	V1467A	probably damaging	Het
Pgm3	A	G	9: 86,562,679 (GRCm38)	S268P	probably benign	Het
Piezo2	A	T	18: 63,074,680 (GRCm38)	I1420N	probably damaging	Het
Pigp	C	A	16: 94,359,147 (GRCm38)	G134V	probably benign	Het
Pik3c2g	T	C	6: 139,843,931 (GRCm38)	Y385H	possibly damaging	Het
Pnliprp2	G	A	19: 58,766,318 (GRCm38)	E265K	probably benign	Het
Psmd13	T	A	7: 140,886,853 (GRCm38)	Y117*	probably null	Het
Ptprq	C	T	10: 107,686,555 (GRCm38)	V546I	probably damaging	Het
Pum1	T	G	4: 130,669,137 (GRCm38)	S68A	probably benign	Het
Rnf167	T	A	11: 70,649,875 (GRCm38)		probably benign	Het
Rnf213	T	C	11: 119,428,157 (GRCm38)	V1148A	possibly damaging	Het
Rnf216	T	C	5: 143,090,316 (GRCm38)	E271G	probably benign	Het
Ros1	A	T	10: 52,154,991 (GRCm38)	I506N	probably damaging	Het
Rpl37	G	A	15: 5,117,646 (GRCm38)	R56Q	possibly damaging	Het
Rpl41	A	C	10: 128,548,707 (GRCm38)		probably benign	Het
Rttn	A	T	18: 89,042,168 (GRCm38)	H998L	probably damaging	Het
Sacs	G	T	14: 61,213,122 (GRCm38)	A4206S	probably damaging	Het
Slc22a14	CTTTCCTGAA	C	9: 119,174,035 (GRCm38)		probably benign	Het
Slc36a3	T	C	11: 55,146,804 (GRCm38)		probably benign	Het
Slc39a3	A	T	10: 81,030,962 (GRCm38)	W317R	probably damaging	Het
Snd1	C	A	6: 28,884,251 (GRCm38)	Y766*	probably null	Het
Snrpd1	T	C	18: 10,626,835 (GRCm38)	V34A	probably benign	Het
Spire2	T	A	8: 123,356,844 (GRCm38)	I189N	probably damaging	Het
Stard6	A	C	18: 70,498,560 (GRCm38)	D74A	possibly damaging	Het
Sufu	C	T	19: 46,475,552 (GRCm38)	T401I	possibly damaging	Het
Taar7b	T	A	10: 24,000,345 (GRCm38)	F136Y	probably benign	Het
Taf6	G	A	5: 138,183,203 (GRCm38)	Q156*	probably null	Het
Tcp11l2	A	G	10: 84,591,163 (GRCm38)	I164V	probably damaging	Het
Tead4	T	C	6: 128,270,987 (GRCm38)	D29G	probably damaging	Het
Tns4	G	T	11: 99,075,213 (GRCm38)	P448Q	probably damaging	Het
Trappc9	G	T	15: 72,937,056 (GRCm38)	N540K	probably damaging	Het
Trbv29	C	T	6: 41,271,854 (GRCm38)	S106F	probably damaging	Het
Usp16	T	A	16: 87,480,914 (GRCm38)	M684K	probably damaging	Het
Usp45	A	T	4: 21,815,372 (GRCm38)	T362S	probably damaging	Het
Vcan	A	T	13: 89,688,842 (GRCm38)	M2861K	probably benign	Het
Vps8	T	C	16: 21,459,786 (GRCm38)	S267P	probably damaging	Het
Wdfy3	C	T	5: 101,943,119 (GRCm38)	D532N	probably benign	Het
Zbtb34	T	A	2: 33,411,614 (GRCm38)	Q305L	probably benign	Het
Zfp454	T	C	11: 50,874,123 (GRCm38)	N161D	probably benign	Het
Zfp804b	A	T	5: 6,771,198 (GRCm38)	S622T	probably damaging	Het
Zswim4	G	T	8: 84,212,223 (GRCm38)	A1010D	probably benign	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90,224,154 (GRCm38)	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90,224,130 (GRCm38)	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90,224,087 (GRCm38)	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90,224,702 (GRCm38)	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90,223,757 (GRCm38)	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90,224,346 (GRCm38)	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90,224,285 (GRCm38)	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90,225,009 (GRCm38)	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90,224,690 (GRCm38)	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90,223,919 (GRCm38)	missense	probably benign
R1034:Zfp352	UTSW	4	90,224,156 (GRCm38)	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90,223,809 (GRCm38)	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90,225,171 (GRCm38)	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90,225,120 (GRCm38)	missense	probably benign	0.08
R2308:Zfp352	UTSW	4	90,225,243 (GRCm38)	missense	probably benign	0.00
R3552:Zfp352	UTSW	4	90,225,102 (GRCm38)	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90,225,149 (GRCm38)	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90,225,149 (GRCm38)	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90,225,024 (GRCm38)	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90,223,834 (GRCm38)	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90,225,164 (GRCm38)	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90,224,535 (GRCm38)	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4617:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90,224,940 (GRCm38)	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90,224,304 (GRCm38)	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90,224,139 (GRCm38)	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90,224,216 (GRCm38)	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90,224,460 (GRCm38)	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90,225,104 (GRCm38)	missense	possibly damaging	0.95
R5942:Zfp352	UTSW	4	90,225,070 (GRCm38)	missense	probably damaging	0.98
R6802:Zfp352	UTSW	4	90,225,200 (GRCm38)	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90,224,699 (GRCm38)	missense	probably benign
R7072:Zfp352	UTSW	4	90,224,424 (GRCm38)	missense	probably benign	0.00
R7099:Zfp352	UTSW	4	90,224,880 (GRCm38)	missense	probably benign	0.00
R7569:Zfp352	UTSW	4	90,223,659 (GRCm38)	missense	possibly damaging	0.77
R7645:Zfp352	UTSW	4	90,224,777 (GRCm38)	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90,225,275 (GRCm38)	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90,224,243 (GRCm38)	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90,224,881 (GRCm38)	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90,224,338 (GRCm38)	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90,224,706 (GRCm38)	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90,224,891 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AACAGCGCCCCTCAGATATG -3'
(R):5'- ATTAACTGCTAGGTTACTCTGCTG -3'

Sequencing Primer
(F):5'- GATATGCCTTCCTCCCCAGAAG -3'
(R):5'- CTCTGCTGATCCTCAAAATAATTCTG -3'

Posted On

2016-04-27