Mutagenetix > Incidental Mutation

Incidental Mutation 'R4943:Vmn2r13'

383254

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r13

Ensembl Gene

ENSMUSG00000091635

Gene Name

vomeronasal 2, receptor 13

Synonyms

Gm4867

MMRRC Submission

042540-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R4943 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109156068-109192107 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 109175049 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Leucine at position 125 (V125L)

Ref Sequence

ENSEMBL: ENSMUSP00000052977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053253]

AlphaFold

L7N1X2

Predicted Effect

probably benign
Transcript: ENSMUST00000053253
AA Change: V125L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000052977
Gene: ENSMUSG00000091635
AA Change: V125L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	76	463	2.8e-29	PFAM
Pfam:NCD3G	506	560	1.3e-18	PFAM
Pfam:7tm_3	593	828	1.8e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.5%

Validation Efficiency

99% (81/82)

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	C	A	13: 77,245,327	T366K	possibly damaging	Het
2510039O18Rik	T	A	4: 147,945,098	H508Q	probably damaging	Het
Actl9	G	A	17: 33,433,085	V40M	possibly damaging	Het
Aen	T	A	7: 78,902,361	V23E	probably damaging	Het
Agbl1	G	A	7: 76,420,016	R432K	probably benign	Het
Akap13	T	A	7: 75,749,240	F2689I	probably benign	Het
Arhgap45	A	G	10: 80,026,503	S475G	probably benign	Het
Atg7	A	C	6: 114,697,084	Q231P	probably benign	Het
Calr3	A	T	8: 72,431,377	V226D	probably benign	Het
Cldn10	G	A	14: 118,788,313	G53S	possibly damaging	Het
Cops4	A	T	5: 100,547,426	M404L	probably benign	Het
Cpne4	A	T	9: 105,019,773	H375L	probably damaging	Het
D16Ertd472e	A	T	16: 78,575,989	V20D	probably damaging	Het
Dcun1d5	T	C	9: 7,186,844	F55L	possibly damaging	Het
Dhx8	T	A	11: 101,737,700	L93*	probably null	Het
Dtx4	A	G	19: 12,501,060	L53P	probably damaging	Het
Ern2	C	T	7: 122,173,258	R659H	possibly damaging	Het
Etl4	G	A	2: 20,807,281	A1392T	probably benign	Het
Fat2	T	C	11: 55,279,033	R2967G	probably benign	Het
Fat4	A	G	3: 38,980,173	D2658G	probably benign	Het
Ftsj3	A	G	11: 106,249,518	V808A	probably damaging	Het
Gm10447	A	T	11: 53,456,389	Y104*	probably null	Het
Gm27013	A	T	6: 130,676,200	C766*	probably null	Het
Gm438	T	A	4: 144,777,720	E287V	probably benign	Het
Gm5581	A	G	6: 131,167,125		noncoding transcript	Het
Gpr158	G	A	2: 21,827,157	V1023I	probably damaging	Het
Hectd2	T	G	19: 36,604,247		probably null	Het
Hmcn2	T	C	2: 31,335,492	Y138H	probably damaging	Het
Kif28	T	C	1: 179,713,951	I369V	probably benign	Het
Kif4-ps	C	T	12: 101,149,217		noncoding transcript	Het
Klk14	G	A	7: 43,692,077	C51Y	probably damaging	Het
Map3k20	G	A	2: 72,371,918	M164I	possibly damaging	Het
Map4k4	A	G	1: 40,019,594	I1050V	probably damaging	Het
Med23	T	A	10: 24,875,669	V133D	possibly damaging	Het
Mycn	A	T	12: 12,937,079	L439Q	probably damaging	Het
Myh2	G	T	11: 67,197,317	A1920S	probably damaging	Het
Myom3	T	C	4: 135,814,274	V1392A	possibly damaging	Het
Nktr	T	A	9: 121,719,954		probably benign	Het
Nme3	A	G	17: 24,896,723	K48E	probably damaging	Het
Nt5dc1	T	C	10: 34,310,391	R58G	probably damaging	Het
Nup205	A	G	6: 35,224,639	E1270G	probably damaging	Het
Olfr106-ps	G	T	17: 37,395,025	A162S	probably benign	Het
Olfr1509	C	G	14: 52,450,594	Y60*	probably null	Het
Olfr630	A	T	7: 103,755,296	F96L	probably benign	Het
Olfr898	C	T	9: 38,349,628	H176Y	probably damaging	Het
Pclo	T	C	5: 14,712,637	L3708P	unknown	Het
Pde4dip	A	T	3: 97,755,511	N590K	probably damaging	Het
Prokr1	A	C	6: 87,581,824	I193S	possibly damaging	Het
Pxdc1	C	A	13: 34,639,006		probably null	Het
Rapgef2	A	T	3: 79,064,547	S1494T	probably benign	Het
Rbms3	G	C	9: 116,678,505		probably benign	Het
Reep3	T	A	10: 67,096,263		probably benign	Het
Rwdd2b	T	C	16: 87,434,534	K244R	possibly damaging	Het
Srrm2	T	C	17: 23,822,415	V2533A	possibly damaging	Het
Stab2	T	C	10: 86,954,162	Y580C	probably damaging	Het
Stac2	G	T	11: 98,041,572	S198R	probably benign	Het
Tdp2	T	A	13: 24,838,265	N222K	probably benign	Het
Tex21	A	G	12: 76,221,700	S103P	probably damaging	Het
Thbs1	A	T	2: 118,113,449	I183F	probably damaging	Het
Tm9sf1	T	C	14: 55,641,168	I256V	probably damaging	Het
Tmem207	C	T	16: 26,517,853	W50*	probably null	Het
Trpm2	C	A	10: 77,966,007	V75L	probably damaging	Het
Vmn1r227	A	T	17: 20,735,361		noncoding transcript	Het
Vmn2r75	A	T	7: 86,165,497	S263T	probably damaging	Het
Wdr64	G	A	1: 175,720,316	V140I	probably benign	Het
Xpo4	T	C	14: 57,638,240	I145M	possibly damaging	Het
Zan	G	A	5: 137,457,890	T1336I	unknown	Het
Zdbf2	G	T	1: 63,302,914	V151F	possibly damaging	Het
Zfhx3	T	C	8: 108,948,317	S2000P	probably damaging	Het
Zfp65	T	A	13: 67,710,980	I12F	probably damaging	Het
Zfp703	C	A	8: 26,979,591	Q428K	probably benign	Het
Zfp947	A	C	17: 22,145,832	M287R	probably benign	Het
Zfp976	A	T	7: 42,612,422		probably benign	Het

Other mutations in Vmn2r13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn2r13	APN	5	109156098	missense	probably damaging	1.00
IGL01373:Vmn2r13	APN	5	109156702	missense	probably damaging	1.00
IGL01946:Vmn2r13	APN	5	109174219	missense	probably benign	0.01
IGL01971:Vmn2r13	APN	5	109174115	missense	probably benign	0.01
IGL02636:Vmn2r13	APN	5	109192017	missense	probably damaging	0.98
IGL03062:Vmn2r13	APN	5	109156282	missense	probably damaging	1.00
IGL03173:Vmn2r13	APN	5	109171779	missense	possibly damaging	0.95
IGL03301:Vmn2r13	APN	5	109158089	missense	probably damaging	0.99
IGL03383:Vmn2r13	APN	5	109156532	missense	probably damaging	0.98
IGL03048:Vmn2r13	UTSW	5	109156285	missense	probably damaging	1.00
R0123:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0134:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0220:Vmn2r13	UTSW	5	109156466	missense	probably damaging	1.00
R0225:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0393:Vmn2r13	UTSW	5	109156529	missense	probably benign	0.01
R0410:Vmn2r13	UTSW	5	109173813	missense	probably benign	0.35
R0787:Vmn2r13	UTSW	5	109156847	missense	probably damaging	0.99
R1200:Vmn2r13	UTSW	5	109174202	missense	probably damaging	1.00
R1448:Vmn2r13	UTSW	5	109174135	missense	probably damaging	1.00
R1782:Vmn2r13	UTSW	5	109158174	missense	probably benign	0.08
R1939:Vmn2r13	UTSW	5	109191986	missense	possibly damaging	0.88
R2029:Vmn2r13	UTSW	5	109192077	missense	probably benign	0.13
R2125:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2126:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2379:Vmn2r13	UTSW	5	109171778	missense	probably benign	0.05
R2680:Vmn2r13	UTSW	5	109174312	missense	possibly damaging	0.66
R2888:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2889:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2890:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R3014:Vmn2r13	UTSW	5	109171761	missense	possibly damaging	0.81
R3683:Vmn2r13	UTSW	5	109156855	missense	probably damaging	1.00
R4074:Vmn2r13	UTSW	5	109156700	missense	probably damaging	1.00
R4599:Vmn2r13	UTSW	5	109156456	missense	probably damaging	1.00
R4614:Vmn2r13	UTSW	5	109175199	missense	probably benign	0.01
R4805:Vmn2r13	UTSW	5	109156465	missense	probably damaging	1.00
R4822:Vmn2r13	UTSW	5	109174072	missense	probably damaging	0.99
R5263:Vmn2r13	UTSW	5	109173975	missense	probably benign	0.00
R5297:Vmn2r13	UTSW	5	109191939	missense	probably benign	0.00
R5502:Vmn2r13	UTSW	5	109173714	missense	probably damaging	1.00
R5554:Vmn2r13	UTSW	5	109191994	missense	possibly damaging	0.49
R5563:Vmn2r13	UTSW	5	109173980	missense	probably benign	0.00
R5819:Vmn2r13	UTSW	5	109174100	missense	possibly damaging	0.79
R6074:Vmn2r13	UTSW	5	109174301	missense	probably benign	0.04
R6416:Vmn2r13	UTSW	5	109174116	missense	probably damaging	0.99
R6419:Vmn2r13	UTSW	5	109175219	missense	possibly damaging	0.87
R6484:Vmn2r13	UTSW	5	109156674	nonsense	probably null
R6486:Vmn2r13	UTSW	5	109156559	missense	probably benign	0.05
R6545:Vmn2r13	UTSW	5	109156940	splice site	probably null
R6700:Vmn2r13	UTSW	5	109175072	missense	probably benign	0.00
R6897:Vmn2r13	UTSW	5	109158149	missense	possibly damaging	0.90
R6957:Vmn2r13	UTSW	5	109156887	nonsense	probably null
R7276:Vmn2r13	UTSW	5	109173779	missense	probably damaging	1.00
R7363:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7443:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7555:Vmn2r13	UTSW	5	109171691	splice site	probably null
R7607:Vmn2r13	UTSW	5	109173640	missense	probably damaging	0.98
R7719:Vmn2r13	UTSW	5	109171752	missense	probably benign	0.00
R8116:Vmn2r13	UTSW	5	109175060	missense	probably benign	0.12
R8242:Vmn2r13	UTSW	5	109175006	missense	possibly damaging	0.65
R8294:Vmn2r13	UTSW	5	109175112	missense	probably benign	0.02
R8340:Vmn2r13	UTSW	5	109174140	missense	probably benign	0.00
R8692:Vmn2r13	UTSW	5	109171648	missense	probably benign	0.03
R8742:Vmn2r13	UTSW	5	109156397	missense	probably benign	0.02
R9022:Vmn2r13	UTSW	5	109156376	missense	possibly damaging	0.94
R9281:Vmn2r13	UTSW	5	109156087	missense	probably damaging	1.00
R9529:Vmn2r13	UTSW	5	109156198	missense	probably damaging	1.00
R9708:Vmn2r13	UTSW	5	109174141	missense	probably benign	0.00
R9746:Vmn2r13	UTSW	5	109191907	critical splice donor site	probably null
X0066:Vmn2r13	UTSW	5	109156219	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- TCTTGTAGGCACATGTAAATGGG -3'
(R):5'- GCCTTGTTTTAGAATACCAGCAAGAAG -3'

Sequencing Primer
(F):5'- TGACTCATCACTACCTGTAAGGGG -3'
(R):5'- CCAGCAAGAAGATATGAGTTTTTCC -3'

Posted On

2016-04-27