Mutagenetix > Incidental Mutation

Incidental Mutation 'R4979:Cacna1e'

384646

Institutional Source

Beutler Lab

Gene Symbol

Cacna1e

Ensembl Gene

ENSMUSG00000004110

Gene Name

calcium channel, voltage-dependent, R type, alpha 1E subunit

Synonyms

Cchra1, alpha1E, Cav2.3

MMRRC Submission

042574-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.151) question?

Stock #

R4979 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

154390731-154884501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 154413993 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1821 (D1821G)

Ref Sequence

ENSEMBL: ENSMUSP00000140937 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004214] [ENSMUST00000187541] [ENSMUST00000211821] [ENSMUST00000226546]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000004214
AA Change: D1513G

PolyPhen 2 Score 0.224 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000004214
Gene: ENSMUSG00000004110
AA Change: D1513G

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	1	55	6.7e-10	PFAM
Pfam:Ion_trans	168	407	3.3e-56	PFAM
Pfam:PKD_channel	257	401	3.3e-7	PFAM
low complexity region	409	414	N/A	INTRINSIC
low complexity region	455	469	N/A	INTRINSIC
low complexity region	496	514	N/A	INTRINSIC
low complexity region	604	620	N/A	INTRINSIC
low complexity region	626	640	N/A	INTRINSIC
coiled coil region	793	823	N/A	INTRINSIC
Pfam:Ion_trans	847	1128	2.3e-63	PFAM
Pfam:Ion_trans	1172	1429	2.6e-65	PFAM
Pfam:PKD_channel	1256	1424	2.8e-10	PFAM
Pfam:GPHH	1431	1500	1.3e-37	PFAM
Ca_chan_IQ	1555	1589	5.93e-13	SMART
low complexity region	1701	1717	N/A	INTRINSIC
low complexity region	1729	1742	N/A	INTRINSIC
low complexity region	1764	1780	N/A	INTRINSIC
low complexity region	1789	1804	N/A	INTRINSIC
low complexity region	1808	1822	N/A	INTRINSIC
low complexity region	1832	1846	N/A	INTRINSIC
low complexity region	1867	1878	N/A	INTRINSIC
low complexity region	1936	1946	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000187541
AA Change: D1821G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000140937
Gene: ENSMUSG00000004110
AA Change: D1821G

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	128	351	8.5e-54	PFAM
PDB:4DEX\|B	354	462	6e-36	PDB
Pfam:Ion_trans	511	703	2.2e-46	PFAM
Pfam:PKD_channel	565	710	1.4e-6	PFAM
low complexity region	717	722	N/A	INTRINSIC
low complexity region	763	777	N/A	INTRINSIC
low complexity region	804	822	N/A	INTRINSIC
low complexity region	912	928	N/A	INTRINSIC
low complexity region	934	948	N/A	INTRINSIC
coiled coil region	1101	1131	N/A	INTRINSIC
low complexity region	1162	1175	N/A	INTRINSIC
Pfam:Ion_trans	1191	1425	4.3e-55	PFAM
Pfam:Ion_trans	1515	1725	5.3e-60	PFAM
Pfam:PKD_channel	1565	1732	4.7e-10	PFAM
Ca_chan_IQ	1863	1897	5.93e-13	SMART
low complexity region	2009	2025	N/A	INTRINSIC
low complexity region	2037	2050	N/A	INTRINSIC
low complexity region	2072	2088	N/A	INTRINSIC
low complexity region	2097	2112	N/A	INTRINSIC
low complexity region	2116	2130	N/A	INTRINSIC
low complexity region	2140	2154	N/A	INTRINSIC
low complexity region	2175	2186	N/A	INTRINSIC
low complexity region	2244	2254	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000211821
AA Change: D1759G

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226546
AA Change: D105G

PolyPhen 2 Score 0.674 (Sensitivity: 0.86; Specificity: 0.92)

Meta Mutation Damage Score

0.2708

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 93.4%

Validation Efficiency

99% (79/80)

MGI Phenotype

FUNCTION: This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit altered R-type Ca2+ channels, increased timidity and body weight, impaired glucose tolerance, reduced locomotor activity, and lack of the cocaine stimulation of locomotor response. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, knock-out(3) Targeted, other(3) Gene trapped(1)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	G	A	17: 9,001,811 (GRCm38)	E381K	probably damaging	Het
Abca1	T	C	4: 53,085,092 (GRCm38)		probably null	Het
Abca7	C	T	10: 80,004,783 (GRCm38)	Q870*	probably null	Het
Ambp	C	T	4: 63,152,651 (GRCm38)	V64M	probably benign	Het
Ank1	T	C	8: 23,132,196 (GRCm38)	V1542A	probably damaging	Het
Anln	T	C	9: 22,376,501 (GRCm38)	Y168C	probably benign	Het
Apoa4	T	A	9: 46,241,505 (GRCm38)	N29K	probably benign	Het
Arfgef1	T	C	1: 10,213,109 (GRCm38)	T192A	probably damaging	Het
Atad2b	G	T	12: 5,034,513 (GRCm38)	D1420Y	probably damaging	Het
Baiap3	A	G	17: 25,246,362 (GRCm38)	W648R	possibly damaging	Het
Bank1	A	G	3: 136,254,901 (GRCm38)	L198P	probably damaging	Het
Bicd2	A	G	13: 49,379,464 (GRCm38)	K509E	possibly damaging	Het
Ccdc80	G	A	16: 45,116,287 (GRCm38)	V692M	possibly damaging	Het
Ccdc88a	C	T	11: 29,482,133 (GRCm38)	Q308*	probably null	Het
Ccl8	T	C	11: 82,116,147 (GRCm38)	V62A	probably damaging	Het
Clspn	C	A	4: 126,578,386 (GRCm38)	P951Q	probably damaging	Het
Cngb1	T	A	8: 95,259,157 (GRCm38)	I858F	probably damaging	Het
Cspp1	T	A	1: 10,126,463 (GRCm38)	N900K	probably damaging	Het
Ctc1	C	T	11: 69,033,502 (GRCm38)	A960V	probably damaging	Het
Ctnnd2	A	G	15: 31,009,075 (GRCm38)	E1106G	probably damaging	Het
Dido1	C	T	2: 180,660,813 (GRCm38)	R1766H	probably damaging	Het
Dnajc13	G	T	9: 104,186,723 (GRCm38)	N1341K	probably damaging	Het
Dnase1l1	C	T	X: 74,277,038 (GRCm38)		probably null	Homo
E2f8	G	A	7: 48,875,170 (GRCm38)		probably benign	Het
Entpd8	A	G	2: 25,082,955 (GRCm38)	D91G	possibly damaging	Het
Fam69a	A	T	5: 107,909,534 (GRCm38)	L386*	probably null	Het
Fars2	A	G	13: 36,204,581 (GRCm38)	R18G	possibly damaging	Het
Fcgbp	A	G	7: 28,117,570 (GRCm38)	S2486G	probably benign	Het
Fibin	C	T	2: 110,362,618 (GRCm38)	D60N	possibly damaging	Het
Fpgs	A	G	2: 32,687,367 (GRCm38)		probably benign	Het
Galnt15	A	G	14: 32,043,290 (GRCm38)	D303G	probably damaging	Het
Gli3	C	A	13: 15,724,464 (GRCm38)	T812K	possibly damaging	Het
Gpbar1	G	C	1: 74,279,245 (GRCm38)	A216P	probably benign	Het
Grin2d	A	G	7: 45,857,933 (GRCm38)	I448T	probably benign	Het
Il21	C	A	3: 37,232,504 (GRCm38)	S21I	probably damaging	Het
Iqce	G	T	5: 140,691,621 (GRCm38)	D148E	probably damaging	Het
Iqcg	T	A	16: 33,019,514 (GRCm38)	E354V	probably damaging	Het
Iws1	T	C	18: 32,093,267 (GRCm38)		probably benign	Het
Ly75	C	T	2: 60,375,894 (GRCm38)	G144S	probably damaging	Het
Marco	C	A	1: 120,494,225 (GRCm38)	M83I	probably benign	Het
Mettl6	A	T	14: 31,479,795 (GRCm38)	L185H	probably damaging	Het
Mppe1	C	T	18: 67,229,702 (GRCm38)	G154D	probably damaging	Het
Mrpl42	T	C	10: 95,490,375 (GRCm38)	E85G	probably benign	Het
Neb	A	G	2: 52,189,909 (GRCm38)	V5518A	probably damaging	Het
Olfr103	A	T	17: 37,336,868 (GRCm38)	F121L	probably benign	Het
Olfr1458	A	T	19: 13,102,689 (GRCm38)	I199N	probably damaging	Het
Olfr656	T	A	7: 104,618,605 (GRCm38)	F317I	probably null	Het
Olfr77	G	T	9: 19,920,359 (GRCm38)	S50I	probably benign	Het
Olfr8	T	A	10: 78,955,932 (GRCm38)	C242*	probably null	Het
Perm1	A	G	4: 156,217,577 (GRCm38)	T193A	probably benign	Het
Prkd2	T	A	7: 16,848,727 (GRCm38)	C172S	probably damaging	Het
Prr23a3	T	A	9: 98,865,378 (GRCm38)	D128E	possibly damaging	Het
Prss28	A	G	17: 25,309,737 (GRCm38)	Y51C	probably damaging	Het
Psmb1	A	T	17: 15,476,189 (GRCm38)	M85K	probably benign	Het
Rae1	T	A	2: 173,012,608 (GRCm38)		probably benign	Het
Rasal1	A	G	5: 120,678,676 (GRCm38)	D759G	probably benign	Het
Rcvrn	G	A	11: 67,695,420 (GRCm38)	G2R	probably damaging	Het
Robo3	C	T	9: 37,423,344 (GRCm38)	A597T	probably damaging	Het
Rsf1	GCG	GCGACGGCGCCG	7: 97,579,907 (GRCm38)		probably benign	Homo
Sdcbp	T	A	4: 6,378,980 (GRCm38)	Y22*	probably null	Het
Sin3a	T	C	9: 57,118,076 (GRCm38)	F1069L	probably damaging	Het
Slitrk3	C	T	3: 73,049,796 (GRCm38)	V548I	possibly damaging	Het
Tbc1d22a	A	G	15: 86,391,086 (GRCm38)	H403R	probably damaging	Het
Tbr1	G	T	2: 61,805,249 (GRCm38)		probably null	Het
Tiam2	T	A	17: 3,505,710 (GRCm38)	D65E	probably damaging	Het
Tpcn2	A	G	7: 145,260,096 (GRCm38)	S488P	probably benign	Het
Trav9-2	T	C	14: 53,591,238 (GRCm38)	S22P	probably damaging	Het
Trim34a	T	A	7: 104,247,862 (GRCm38)	N44K	probably benign	Het
Unc79	C	G	12: 103,112,432 (GRCm38)	P1619A	probably benign	Het
Usp22	A	T	11: 61,157,216 (GRCm38)	V426E	probably damaging	Het
Vhl	A	T	6: 113,624,198 (GRCm38)	M20L	unknown	Het
Vmn1r215	G	A	13: 23,075,894 (GRCm38)	A35T	probably benign	Het
Vmn1r222	G	A	13: 23,232,432 (GRCm38)	L204F	possibly damaging	Het
Zfp871	A	T	17: 32,775,855 (GRCm38)	H115Q	probably damaging	Het
Zpr1	T	A	9: 46,278,342 (GRCm38)	F340L	probably benign	Het

Other mutations in Cacna1e

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Cacna1e	APN	1	154,403,683 (GRCm38)	missense	probably damaging	0.99
IGL01086:Cacna1e	APN	1	154,471,601 (GRCm38)	missense	probably benign	0.04
IGL01302:Cacna1e	APN	1	154,443,907 (GRCm38)	missense	probably damaging	1.00
IGL01386:Cacna1e	APN	1	154,472,377 (GRCm38)	missense	probably benign	0.18
IGL01573:Cacna1e	APN	1	154,471,367 (GRCm38)	missense	probably benign
IGL01676:Cacna1e	APN	1	154,412,450 (GRCm38)	missense	probably damaging	1.00
IGL01676:Cacna1e	APN	1	154,398,476 (GRCm38)	missense	probably damaging	1.00
IGL01762:Cacna1e	APN	1	154,471,373 (GRCm38)	missense	possibly damaging	0.78
IGL01801:Cacna1e	APN	1	154,471,340 (GRCm38)	missense	probably null	0.00
IGL01895:Cacna1e	APN	1	154,443,900 (GRCm38)	missense	probably damaging	1.00
IGL02391:Cacna1e	APN	1	154,421,113 (GRCm38)	missense	probably damaging	1.00
IGL02399:Cacna1e	APN	1	154,403,747 (GRCm38)	missense	probably damaging	1.00
IGL02659:Cacna1e	APN	1	154,426,528 (GRCm38)	missense	probably damaging	1.00
IGL02686:Cacna1e	APN	1	154,493,409 (GRCm38)	missense	probably damaging	1.00
IGL02838:Cacna1e	APN	1	154,445,648 (GRCm38)	missense	probably damaging	1.00
IGL02958:Cacna1e	APN	1	154,465,741 (GRCm38)	missense	probably damaging	1.00
IGL02981:Cacna1e	APN	1	154,471,425 (GRCm38)	missense	probably benign	0.15
IGL03120:Cacna1e	APN	1	154,443,881 (GRCm38)	missense	probably damaging	1.00
IGL03232:Cacna1e	APN	1	154,493,358 (GRCm38)	missense	probably damaging	1.00
IGL03310:Cacna1e	APN	1	154,442,251 (GRCm38)	missense	probably damaging	1.00
IGL03342:Cacna1e	APN	1	154,466,944 (GRCm38)	critical splice donor site	probably null
bezoar	UTSW	1	154,436,554 (GRCm38)	splice site	probably null
hairball	UTSW	1	154,479,305 (GRCm38)	missense	probably damaging	0.97
N/A - 535:Cacna1e	UTSW	1	154,465,764 (GRCm38)	missense	probably damaging	1.00
R0122:Cacna1e	UTSW	1	154,443,901 (GRCm38)	missense	probably damaging	1.00
R0143:Cacna1e	UTSW	1	154,448,947 (GRCm38)	splice site	probably null
R0314:Cacna1e	UTSW	1	154,442,251 (GRCm38)	missense	probably damaging	1.00
R0366:Cacna1e	UTSW	1	154,416,138 (GRCm38)	missense	probably benign	0.03
R0626:Cacna1e	UTSW	1	154,488,817 (GRCm38)	missense	probably damaging	0.99
R0739:Cacna1e	UTSW	1	154,442,278 (GRCm38)	missense	probably damaging	0.97
R1272:Cacna1e	UTSW	1	154,444,968 (GRCm38)	missense	probably damaging	1.00
R1300:Cacna1e	UTSW	1	154,398,673 (GRCm38)	missense	probably benign
R1340:Cacna1e	UTSW	1	154,472,657 (GRCm38)	missense	probably damaging	1.00
R1440:Cacna1e	UTSW	1	154,561,806 (GRCm38)	missense	possibly damaging	0.63
R1449:Cacna1e	UTSW	1	154,485,662 (GRCm38)	critical splice donor site	probably null
R1538:Cacna1e	UTSW	1	154,561,758 (GRCm38)	missense	probably damaging	0.99
R1542:Cacna1e	UTSW	1	154,477,779 (GRCm38)	missense	probably benign	0.01
R1560:Cacna1e	UTSW	1	154,421,104 (GRCm38)	nonsense	probably null
R1748:Cacna1e	UTSW	1	154,486,569 (GRCm38)	missense	possibly damaging	0.92
R1749:Cacna1e	UTSW	1	154,444,000 (GRCm38)	missense	probably damaging	1.00
R1912:Cacna1e	UTSW	1	154,436,449 (GRCm38)	missense	probably damaging	1.00
R1968:Cacna1e	UTSW	1	154,700,494 (GRCm38)	missense	probably damaging	1.00
R1993:Cacna1e	UTSW	1	154,477,817 (GRCm38)	missense	probably damaging	0.97
R1994:Cacna1e	UTSW	1	154,477,817 (GRCm38)	missense	probably damaging	0.97
R2191:Cacna1e	UTSW	1	154,443,845 (GRCm38)	missense	probably damaging	1.00
R2291:Cacna1e	UTSW	1	154,403,683 (GRCm38)	missense	probably damaging	0.99
R2417:Cacna1e	UTSW	1	154,472,193 (GRCm38)	missense	probably damaging	1.00
R3608:Cacna1e	UTSW	1	154,416,085 (GRCm38)	missense	probably benign	0.08
R3757:Cacna1e	UTSW	1	154,633,696 (GRCm38)	missense	probably damaging	0.97
R3890:Cacna1e	UTSW	1	154,483,553 (GRCm38)	missense	probably damaging	1.00
R4015:Cacna1e	UTSW	1	154,482,585 (GRCm38)	missense	probably damaging	1.00
R4088:Cacna1e	UTSW	1	154,412,183 (GRCm38)	splice site	probably null
R4275:Cacna1e	UTSW	1	154,493,325 (GRCm38)	missense	probably damaging	1.00
R4282:Cacna1e	UTSW	1	154,426,550 (GRCm38)	missense	probably benign	0.04
R4297:Cacna1e	UTSW	1	154,398,731 (GRCm38)	missense	probably benign	0.37
R4356:Cacna1e	UTSW	1	154,443,981 (GRCm38)	missense	probably damaging	1.00
R4510:Cacna1e	UTSW	1	154,561,833 (GRCm38)	missense	probably damaging	1.00
R4511:Cacna1e	UTSW	1	154,561,833 (GRCm38)	missense	probably damaging	1.00
R4577:Cacna1e	UTSW	1	154,402,027 (GRCm38)	missense	possibly damaging	0.92
R4590:Cacna1e	UTSW	1	154,436,519 (GRCm38)	missense	possibly damaging	0.87
R4601:Cacna1e	UTSW	1	154,471,613 (GRCm38)	missense	probably benign
R4622:Cacna1e	UTSW	1	154,471,565 (GRCm38)	missense	possibly damaging	0.81
R4626:Cacna1e	UTSW	1	154,482,548 (GRCm38)	splice site	probably null
R4694:Cacna1e	UTSW	1	154,437,266 (GRCm38)	critical splice donor site	probably null
R4727:Cacna1e	UTSW	1	154,436,468 (GRCm38)	nonsense	probably null
R4839:Cacna1e	UTSW	1	154,421,058 (GRCm38)	missense	probably damaging	1.00
R4851:Cacna1e	UTSW	1	154,436,554 (GRCm38)	splice site	probably null
R4894:Cacna1e	UTSW	1	154,488,805 (GRCm38)	nonsense	probably null
R4934:Cacna1e	UTSW	1	154,481,634 (GRCm38)	nonsense	probably null
R5077:Cacna1e	UTSW	1	154,561,729 (GRCm38)	critical splice donor site	probably null
R5128:Cacna1e	UTSW	1	154,402,021 (GRCm38)	missense	probably damaging	0.98
R5214:Cacna1e	UTSW	1	154,701,364 (GRCm38)	missense	possibly damaging	0.93
R5274:Cacna1e	UTSW	1	154,700,504 (GRCm38)	missense	probably damaging	0.98
R5388:Cacna1e	UTSW	1	154,477,796 (GRCm38)	missense	probably damaging	1.00
R5416:Cacna1e	UTSW	1	154,465,779 (GRCm38)	missense	probably damaging	1.00
R5469:Cacna1e	UTSW	1	154,443,937 (GRCm38)	missense	probably damaging	1.00
R5475:Cacna1e	UTSW	1	154,725,709 (GRCm38)	missense	possibly damaging	0.53
R5607:Cacna1e	UTSW	1	154,471,340 (GRCm38)	missense	probably benign	0.00
R5615:Cacna1e	UTSW	1	154,412,170 (GRCm38)	missense	probably damaging	1.00
R5616:Cacna1e	UTSW	1	154,442,194 (GRCm38)	missense	probably damaging	1.00
R5627:Cacna1e	UTSW	1	154,635,858 (GRCm38)	missense	probably damaging	0.98
R5707:Cacna1e	UTSW	1	154,633,717 (GRCm38)	missense	probably damaging	1.00
R5756:Cacna1e	UTSW	1	154,471,637 (GRCm38)	missense	probably benign	0.00
R5893:Cacna1e	UTSW	1	154,437,323 (GRCm38)	missense	probably damaging	1.00
R6117:Cacna1e	UTSW	1	154,561,791 (GRCm38)	missense	possibly damaging	0.68
R6134:Cacna1e	UTSW	1	154,701,291 (GRCm38)	missense	probably damaging	1.00
R6190:Cacna1e	UTSW	1	154,486,570 (GRCm38)	missense	possibly damaging	0.47
R6279:Cacna1e	UTSW	1	154,425,932 (GRCm38)	missense	probably benign	0.38
R6295:Cacna1e	UTSW	1	154,442,173 (GRCm38)	missense	probably damaging	0.98
R6300:Cacna1e	UTSW	1	154,425,932 (GRCm38)	missense	probably benign	0.38
R6320:Cacna1e	UTSW	1	154,441,524 (GRCm38)	missense	possibly damaging	0.76
R6375:Cacna1e	UTSW	1	154,479,305 (GRCm38)	missense	probably damaging	0.97
R6830:Cacna1e	UTSW	1	154,413,974 (GRCm38)	critical splice donor site	probably null
R6842:Cacna1e	UTSW	1	154,483,117 (GRCm38)	missense	probably damaging	1.00
R7023:Cacna1e	UTSW	1	154,725,693 (GRCm38)	missense	probably null	0.85
R7081:Cacna1e	UTSW	1	154,700,383 (GRCm38)	missense	possibly damaging	0.82
R7085:Cacna1e	UTSW	1	154,473,746 (GRCm38)	splice site	probably null
R7108:Cacna1e	UTSW	1	154,468,995 (GRCm38)	frame shift	probably null
R7142:Cacna1e	UTSW	1	154,412,484 (GRCm38)	missense	probably damaging	1.00
R7250:Cacna1e	UTSW	1	154,700,489 (GRCm38)	missense	possibly damaging	0.93
R7332:Cacna1e	UTSW	1	154,725,801 (GRCm38)	missense	possibly damaging	0.89
R7410:Cacna1e	UTSW	1	154,472,234 (GRCm38)	missense	probably benign	0.13
R7502:Cacna1e	UTSW	1	154,468,988 (GRCm38)	missense	probably null	0.35
R7556:Cacna1e	UTSW	1	154,472,673 (GRCm38)	missense	probably benign	0.28
R7563:Cacna1e	UTSW	1	154,471,416 (GRCm38)	missense	probably benign	0.00
R7573:Cacna1e	UTSW	1	154,726,165 (GRCm38)	intron	probably benign
R7689:Cacna1e	UTSW	1	154,398,803 (GRCm38)	missense	probably benign	0.01
R7699:Cacna1e	UTSW	1	154,443,928 (GRCm38)	missense	probably damaging	1.00
R7744:Cacna1e	UTSW	1	154,465,792 (GRCm38)	missense	probably damaging	1.00
R7754:Cacna1e	UTSW	1	154,413,117 (GRCm38)	missense	probably damaging	0.97
R7787:Cacna1e	UTSW	1	154,482,568 (GRCm38)	missense	probably damaging	0.98
R7818:Cacna1e	UTSW	1	154,398,406 (GRCm38)	missense	probably damaging	1.00
R7838:Cacna1e	UTSW	1	154,471,403 (GRCm38)	missense	probably benign	0.08
R7849:Cacna1e	UTSW	1	154,633,718 (GRCm38)	missense	probably damaging	1.00
R8011:Cacna1e	UTSW	1	154,465,822 (GRCm38)	missense	probably benign	0.01
R8094:Cacna1e	UTSW	1	154,561,770 (GRCm38)	missense	probably damaging	1.00
R8162:Cacna1e	UTSW	1	154,701,567 (GRCm38)	splice site	probably null
R8202:Cacna1e	UTSW	1	154,398,449 (GRCm38)	missense	probably benign
R8280:Cacna1e	UTSW	1	154,469,093 (GRCm38)	missense	probably damaging	0.97
R8354:Cacna1e	UTSW	1	154,398,568 (GRCm38)	missense	probably damaging	1.00
R8385:Cacna1e	UTSW	1	154,443,941 (GRCm38)	missense	probably damaging	0.98
R8532:Cacna1e	UTSW	1	154,465,764 (GRCm38)	missense	probably damaging	1.00
R8902:Cacna1e	UTSW	1	154,473,886 (GRCm38)	missense	probably benign	0.01
R8926:Cacna1e	UTSW	1	154,701,334 (GRCm38)	missense	possibly damaging	0.84
R8947:Cacna1e	UTSW	1	154,402,150 (GRCm38)	missense	probably benign	0.10
R9094:Cacna1e	UTSW	1	154,479,318 (GRCm38)	missense	possibly damaging	0.93
R9126:Cacna1e	UTSW	1	154,467,764 (GRCm38)	missense	probably benign	0.01
R9175:Cacna1e	UTSW	1	154,398,568 (GRCm38)	missense	probably damaging	1.00
R9286:Cacna1e	UTSW	1	154,413,099 (GRCm38)	missense	probably damaging	1.00
R9377:Cacna1e	UTSW	1	154,485,712 (GRCm38)	missense	possibly damaging	0.88
R9452:Cacna1e	UTSW	1	154,413,974 (GRCm38)	critical splice donor site	probably null
R9463:Cacna1e	UTSW	1	154,481,665 (GRCm38)	missense	probably damaging	1.00
R9513:Cacna1e	UTSW	1	154,442,287 (GRCm38)	missense	probably damaging	1.00
R9534:Cacna1e	UTSW	1	154,444,947 (GRCm38)	missense	possibly damaging	0.65
R9562:Cacna1e	UTSW	1	154,407,740 (GRCm38)	missense	probably benign	0.01
RF008:Cacna1e	UTSW	1	154,442,136 (GRCm38)	missense	probably damaging	1.00
X0062:Cacna1e	UTSW	1	154,412,492 (GRCm38)	missense	probably damaging	1.00
Z1176:Cacna1e	UTSW	1	154,635,850 (GRCm38)	missense	probably damaging	0.98
Z1177:Cacna1e	UTSW	1	154,442,292 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCAGGCATGGAATAGCAAGTC -3'
(R):5'- AGCTTTAGTGAGAAGCTGGG -3'

Sequencing Primer
(F):5'- TTTACCAAAGTAAGGACAAGTCTTC -3'
(R):5'- CGGACTGGCATGGGGATG -3'

Posted On

2016-05-10