Mutagenetix > Incidental Mutation

Incidental Mutation 'R4979:Olfr656'

384672

Institutional Source

Beutler Lab

Gene Symbol

Olfr656

Ensembl Gene

ENSMUSG00000073924

Gene Name

olfactory receptor 656

Synonyms

GA_x6K02T2PBJ9-7245486-7246451, MOR27-1

MMRRC Submission

042574-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R4979 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

104613955-104621603 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 104618605 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 317 (F317I)

Ref Sequence

ENSEMBL: ENSMUSP00000095774 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098172] [ENSMUST00000215575]

AlphaFold

Q8VGX9

Predicted Effect

probably null
Transcript: ENSMUST00000098172
AA Change: F317I

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000095774
Gene: ENSMUSG00000073924
AA Change: F317I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	319	5.2e-105	PFAM
Pfam:7TM_GPCR_Srsx	42	316	1.1e-8	PFAM
Pfam:7tm_1	51	302	4.9e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210687
AA Change: F309I

Predicted Effect

probably benign
Transcript: ENSMUST00000215575
AA Change: F309I

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 93.4%

Validation Efficiency

99% (79/80)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	G	A	17: 9,001,811	E381K	probably damaging	Het
Abca1	T	C	4: 53,085,092		probably null	Het
Abca7	C	T	10: 80,004,783	Q870*	probably null	Het
Ambp	C	T	4: 63,152,651	V64M	probably benign	Het
Ank1	T	C	8: 23,132,196	V1542A	probably damaging	Het
Anln	T	C	9: 22,376,501	Y168C	probably benign	Het
Apoa4	T	A	9: 46,241,505	N29K	probably benign	Het
Arfgef1	T	C	1: 10,213,109	T192A	probably damaging	Het
Atad2b	G	T	12: 5,034,513	D1420Y	probably damaging	Het
Baiap3	A	G	17: 25,246,362	W648R	possibly damaging	Het
Bank1	A	G	3: 136,254,901	L198P	probably damaging	Het
Bicd2	A	G	13: 49,379,464	K509E	possibly damaging	Het
Cacna1e	T	C	1: 154,413,993	D1821G	probably damaging	Het
Ccdc80	G	A	16: 45,116,287	V692M	possibly damaging	Het
Ccdc88a	C	T	11: 29,482,133	Q308*	probably null	Het
Ccl8	T	C	11: 82,116,147	V62A	probably damaging	Het
Clspn	C	A	4: 126,578,386	P951Q	probably damaging	Het
Cngb1	T	A	8: 95,259,157	I858F	probably damaging	Het
Cspp1	T	A	1: 10,126,463	N900K	probably damaging	Het
Ctc1	C	T	11: 69,033,502	A960V	probably damaging	Het
Ctnnd2	A	G	15: 31,009,075	E1106G	probably damaging	Het
Dido1	C	T	2: 180,660,813	R1766H	probably damaging	Het
Dnajc13	G	T	9: 104,186,723	N1341K	probably damaging	Het
Dnase1l1	C	T	X: 74,277,038		probably null	Homo
E2f8	G	A	7: 48,875,170		probably benign	Het
Entpd8	A	G	2: 25,082,955	D91G	possibly damaging	Het
Fam69a	A	T	5: 107,909,534	L386*	probably null	Het
Fars2	A	G	13: 36,204,581	R18G	possibly damaging	Het
Fcgbp	A	G	7: 28,117,570	S2486G	probably benign	Het
Fibin	C	T	2: 110,362,618	D60N	possibly damaging	Het
Fpgs	A	G	2: 32,687,367		probably benign	Het
Galnt15	A	G	14: 32,043,290	D303G	probably damaging	Het
Gli3	C	A	13: 15,724,464	T812K	possibly damaging	Het
Gpbar1	G	C	1: 74,279,245	A216P	probably benign	Het
Grin2d	A	G	7: 45,857,933	I448T	probably benign	Het
Il21	C	A	3: 37,232,504	S21I	probably damaging	Het
Iqce	G	T	5: 140,691,621	D148E	probably damaging	Het
Iqcg	T	A	16: 33,019,514	E354V	probably damaging	Het
Iws1	T	C	18: 32,093,267		probably benign	Het
Ly75	C	T	2: 60,375,894	G144S	probably damaging	Het
Marco	C	A	1: 120,494,225	M83I	probably benign	Het
Mettl6	A	T	14: 31,479,795	L185H	probably damaging	Het
Mppe1	C	T	18: 67,229,702	G154D	probably damaging	Het
Mrpl42	T	C	10: 95,490,375	E85G	probably benign	Het
Neb	A	G	2: 52,189,909	V5518A	probably damaging	Het
Olfr103	A	T	17: 37,336,868	F121L	probably benign	Het
Olfr1458	A	T	19: 13,102,689	I199N	probably damaging	Het
Olfr77	G	T	9: 19,920,359	S50I	probably benign	Het
Olfr8	T	A	10: 78,955,932	C242*	probably null	Het
Perm1	A	G	4: 156,217,577	T193A	probably benign	Het
Prkd2	T	A	7: 16,848,727	C172S	probably damaging	Het
Prr23a3	T	A	9: 98,865,378	D128E	possibly damaging	Het
Prss28	A	G	17: 25,309,737	Y51C	probably damaging	Het
Psmb1	A	T	17: 15,476,189	M85K	probably benign	Het
Rae1	T	A	2: 173,012,608		probably benign	Het
Rasal1	A	G	5: 120,678,676	D759G	probably benign	Het
Rcvrn	G	A	11: 67,695,420	G2R	probably damaging	Het
Robo3	C	T	9: 37,423,344	A597T	probably damaging	Het
Rsf1	GCG	GCGACGGCGCCG	7: 97,579,907		probably benign	Homo
Sdcbp	T	A	4: 6,378,980	Y22*	probably null	Het
Sin3a	T	C	9: 57,118,076	F1069L	probably damaging	Het
Slitrk3	C	T	3: 73,049,796	V548I	possibly damaging	Het
Tbc1d22a	A	G	15: 86,391,086	H403R	probably damaging	Het
Tbr1	G	T	2: 61,805,249		probably null	Het
Tiam2	T	A	17: 3,505,710	D65E	probably damaging	Het
Tpcn2	A	G	7: 145,260,096	S488P	probably benign	Het
Trav9-2	T	C	14: 53,591,238	S22P	probably damaging	Het
Trim34a	T	A	7: 104,247,862	N44K	probably benign	Het
Unc79	C	G	12: 103,112,432	P1619A	probably benign	Het
Usp22	A	T	11: 61,157,216	V426E	probably damaging	Het
Vhl	A	T	6: 113,624,198	M20L	unknown	Het
Vmn1r215	G	A	13: 23,075,894	A35T	probably benign	Het
Vmn1r222	G	A	13: 23,232,432	L204F	possibly damaging	Het
Zfp871	A	T	17: 32,775,855	H115Q	probably damaging	Het
Zpr1	T	A	9: 46,278,342	F340L	probably benign	Het

Other mutations in Olfr656

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01616:Olfr656	APN	7	104617721	missense	probably damaging	0.98
IGL01908:Olfr656	APN	7	104617699	missense	probably damaging	1.00
IGL02695:Olfr656	APN	7	104618471	missense	probably damaging	1.00
IGL03055:Olfr656	UTSW	7	104618206	missense	probably damaging	1.00
R0128:Olfr656	UTSW	7	104618581	missense	probably damaging	1.00
R0184:Olfr656	UTSW	7	104618240	missense	probably damaging	1.00
R4674:Olfr656	UTSW	7	104618424	nonsense	probably null
R4675:Olfr656	UTSW	7	104618424	nonsense	probably null
R4723:Olfr656	UTSW	7	104618489	missense	possibly damaging	0.56
R6273:Olfr656	UTSW	7	104617895	missense	probably damaging	1.00
R6359:Olfr656	UTSW	7	104618303	missense	probably damaging	1.00
R6582:Olfr656	UTSW	7	104618441	missense	probably damaging	1.00
R6750:Olfr656	UTSW	7	104618113	missense	probably damaging	0.99
R7426:Olfr656	UTSW	7	104617852	missense	probably damaging	1.00
R7786:Olfr656	UTSW	7	104617718	missense	probably benign	0.02
R8068:Olfr656	UTSW	7	104618253	nonsense	probably null
R8324:Olfr656	UTSW	7	104618114	missense	probably benign	0.00
R8486:Olfr656	UTSW	7	104617862	missense	possibly damaging	0.47
R8828:Olfr656	UTSW	7	104618279	missense	probably damaging	1.00
R8933:Olfr656	UTSW	7	104617666	missense	probably benign	0.05
R8971:Olfr656	UTSW	7	104618260	missense	probably damaging	1.00
R9329:Olfr656	UTSW	7	104618498	missense	probably damaging	1.00
X0065:Olfr656	UTSW	7	104617758	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCGACTCTCATCCCAGGAAG -3'
(R):5'- GAACAGAAGCTTTTCAGAGGATC -3'

Sequencing Primer
(F):5'- GTCCAAAGCCTTAGGTACCTGTG -3'
(R):5'- CAGAAGCTTTTCAGAGGATCTTATC -3'

Posted On

2016-05-10