Mutagenetix > Incidental Mutation

Incidental Mutation 'R4982:Dmrta1'

384903

Institutional Source

Beutler Lab

Gene Symbol

Dmrta1

Ensembl Gene

ENSMUSG00000043753

Gene Name

doublesex and mab-3 related transcription factor like family A1

Synonyms

Dmrt4

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4982 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

89679436-89694772 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 89688564 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 86 (C86R)

Ref Sequence

ENSEMBL: ENSMUSP00000057488 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052478]

AlphaFold

Q8CFG4

Predicted Effect

probably damaging
Transcript: ENSMUST00000052478
AA Change: C86R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000057488
Gene: ENSMUSG00000043753
AA Change: C86R

Domain	Start	End	E-Value	Type
low complexity region	20	37	N/A	INTRINSIC
DM	82	135	2.31e-30	SMART
low complexity region	151	165	N/A	INTRINSIC
Pfam:DMA	314	350	3.3e-21	PFAM
low complexity region	393	404	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131576

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele are viable and fertile; however, females exhibit polyovular ovarian follicles while 25% of males display abnormal copulatory behavior toward other males. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,292,348	I1404L	possibly damaging	Het
Adra1b	A	G	11: 43,835,230	S287P	probably damaging	Het
Atxn2	G	T	5: 121,814,343	A1280S	possibly damaging	Het
Bbip1	T	C	19: 53,932,208		probably null	Het
Bbs2	A	G	8: 94,082,354		probably null	Het
Bcl11b	A	T	12: 107,965,772	C180*	probably null	Het
Bod1l	A	G	5: 41,820,473	V1166A	probably benign	Het
Bora	C	T	14: 99,047,352	P13S	probably damaging	Het
C2cd4c	T	C	10: 79,613,241	E24G	probably benign	Het
Ccne1	A	T	7: 38,100,571	I196N	probably damaging	Het
Chsy3	C	T	18: 59,409,575	S595L	probably benign	Het
Chsy3	T	A	18: 59,409,767	I659N	possibly damaging	Het
Cntrob	T	A	11: 69,311,362		probably null	Het
Col5a2	G	A	1: 45,389,458	P983S	possibly damaging	Het
Crat	T	A	2: 30,407,136		probably null	Het
Ctnnbl1	C	T	2: 157,836,553	H359Y	probably benign	Het
D430041D05Rik	A	G	2: 104,255,387	V83A	possibly damaging	Het
Dixdc1	A	G	9: 50,682,602	S488P	possibly damaging	Het
Dnase1l1	C	T	X: 74,277,038		probably null	Homo
Fam171a1	G	A	2: 3,178,468		probably null	Het
Fam222b	T	C	11: 78,154,743	C249R	probably damaging	Het
Fbxw18	T	A	9: 109,702,651		probably benign	Het
Fes	T	C	7: 80,387,204	Y44C	probably damaging	Het
Gimap6	A	G	6: 48,707,999	V51A	probably benign	Het
Gpr75	C	A	11: 30,891,462	H122Q	probably damaging	Het
Gpr75	C	T	11: 30,891,463	L123F	possibly damaging	Het
Greb1	A	G	12: 16,724,761	S212P	probably damaging	Het
Grin3a	T	C	4: 49,665,512	H1041R	probably benign	Het
Ifna5	A	G	4: 88,835,624	N34D	probably damaging	Het
Ift140	A	T	17: 25,036,994	H221L	probably damaging	Het
Igsf3	T	C	3: 101,435,667	V540A	probably benign	Het
Il10ra	A	G	9: 45,269,059	L5S	probably damaging	Het
Klra2	A	T	6: 131,220,189	D282E	probably benign	Het
Lyst	T	A	13: 13,725,954	H3138Q	probably damaging	Het
Malrd1	A	G	2: 16,042,129	T1689A	probably benign	Het
Mon2	A	T	10: 122,995,789	L1671M	probably damaging	Het
Mpped1	T	C	15: 83,836,327	F71S	probably damaging	Het
Mtpap	C	A	18: 4,396,332	H541Q	probably benign	Het
Muc5ac	T	A	7: 141,809,456		probably benign	Het
Mybbp1a	T	C	11: 72,445,214	I451T	probably damaging	Het
Myh7	T	A	14: 54,972,767	E1827V	probably damaging	Het
Olfr132	A	T	17: 38,130,577	I205N	probably damaging	Het
Olfr145	C	A	9: 37,897,515	T37N	probably damaging	Het
Olfr342	A	T	2: 36,527,397		probably null	Het
Olfr501-ps1	T	A	7: 108,508,648	Y197*	probably null	Het
Os9	C	T	10: 127,121,051	R23H	possibly damaging	Het
Otud6b	A	G	4: 14,815,607	L261P	probably damaging	Het
Pcdhga2	A	G	18: 37,669,423	N107D	probably benign	Het
Pclo	C	T	5: 14,679,294		probably benign	Het
Peg10	T	TCCG	6: 4,756,451		probably benign	Het
Phtf1	T	A	3: 103,998,708	S524T	probably damaging	Het
Pkdrej	A	C	15: 85,818,996	L913R	probably damaging	Het
Pld1	C	A	3: 28,031,298	A201D	probably damaging	Het
Rorb	T	A	19: 18,977,688	Q103L	probably benign	Het
Sec24d	C	T	3: 123,299,606	T284M	probably benign	Het
Serpinb3b	T	C	1: 107,157,754	I86V	probably benign	Het
Serpinb6a	A	T	13: 33,918,874	M201K	probably damaging	Het
Snx8	A	G	5: 140,352,234	S219P	probably benign	Het
Sp8	C	T	12: 118,848,425	T5I	probably damaging	Het
Tanc1	A	G	2: 59,799,943	N749D	probably damaging	Het
Tarm1	G	C	7: 3,489,096	P284A	probably damaging	Het
Tbx15	A	T	3: 99,254,074	E65V	probably benign	Het
Ticam1	T	A	17: 56,272,020	H25L	probably benign	Het
Tmprss11g	T	A	5: 86,492,815	L170F	probably damaging	Het
Tnfsf9	A	G	17: 57,107,504	*310W	probably null	Het
Tsks	C	T	7: 44,943,994	T128I	possibly damaging	Het
Uhrf1bp1	T	C	17: 27,886,606	F702S	probably benign	Het
Vmn1r175	A	T	7: 23,809,069	N44K	possibly damaging	Het
Vmn1r45	A	G	6: 89,933,865	I41T	probably damaging	Het
Ythdc2	A	G	18: 44,871,465	N1102S	probably benign	Het
Zswim4	C	T	8: 84,226,667		probably null	Het

Other mutations in Dmrta1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02154:Dmrta1	APN	4	89691913	missense	probably benign	0.04
IGL02572:Dmrta1	APN	4	89691558	missense	probably benign	0.01
IGL02875:Dmrta1	APN	4	89691748	missense	possibly damaging	0.70
IGL02883:Dmrta1	APN	4	89688774	missense	probably benign
R0097:Dmrta1	UTSW	4	89688872	missense	probably benign	0.08
R0097:Dmrta1	UTSW	4	89688872	missense	probably benign	0.08
R0394:Dmrta1	UTSW	4	89692039	missense	probably damaging	1.00
R2093:Dmrta1	UTSW	4	89691505	missense	probably benign
R2132:Dmrta1	UTSW	4	89688709	missense	probably damaging	1.00
R2133:Dmrta1	UTSW	4	89688709	missense	probably damaging	1.00
R2401:Dmrta1	UTSW	4	89691616	missense	probably benign	0.00
R3694:Dmrta1	UTSW	4	89692178	nonsense	probably null
R3695:Dmrta1	UTSW	4	89692178	nonsense	probably null
R3891:Dmrta1	UTSW	4	89691594	missense	possibly damaging	0.93
R3892:Dmrta1	UTSW	4	89691594	missense	possibly damaging	0.93
R3929:Dmrta1	UTSW	4	89691444	nonsense	probably null
R4620:Dmrta1	UTSW	4	89688784	missense	probably benign	0.05
R4927:Dmrta1	UTSW	4	89691748	missense	probably damaging	1.00
R5312:Dmrta1	UTSW	4	89692047	missense	probably damaging	0.99
R5437:Dmrta1	UTSW	4	89691756	missense	possibly damaging	0.71
R5637:Dmrta1	UTSW	4	89688831	missense	probably benign
R6185:Dmrta1	UTSW	4	89691768	missense	probably damaging	0.97
R6906:Dmrta1	UTSW	4	89691966	missense	probably benign	0.06
R7156:Dmrta1	UTSW	4	89688463	missense	probably damaging	0.99
R7201:Dmrta1	UTSW	4	89692171	nonsense	probably null
R7755:Dmrta1	UTSW	4	89691933	missense	probably benign	0.01
R7862:Dmrta1	UTSW	4	89688324	missense	probably benign	0.00
R7880:Dmrta1	UTSW	4	89688844	missense	possibly damaging	0.95
R8714:Dmrta1	UTSW	4	89691445	missense	probably benign
R8841:Dmrta1	UTSW	4	89691713	missense	probably benign	0.00
R9028:Dmrta1	UTSW	4	89691677	missense	probably damaging	0.96
Z1177:Dmrta1	UTSW	4	89688408	missense	probably damaging	0.97
Z1177:Dmrta1	UTSW	4	89688454	missense	probably benign	0.37
Z1177:Dmrta1	UTSW	4	89688498	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- CGATTACCTCACGGTCGTAGAG -3'
(R):5'- TATTCAAGACCTGGGGACTCTC -3'

Sequencing Primer
(F):5'- TCGTAGAGACCGCAGCG -3'
(R):5'- AGAAGCCTGTGCAGCCC -3'

Posted On

2016-05-10