Incidental Mutation 'R0374:Wls'
ID 38505
Institutional Source Beutler Lab
Gene Symbol Wls
Ensembl Gene ENSMUSG00000028173
Gene Name wntless WNT ligand secretion mediator
Synonyms 5031439A09Rik, Gpr177
MMRRC Submission 038580-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0374 (G1)
Quality Score 225
Status Validated
Chromosome 3
Chromosomal Location 159545309-159644300 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) T to A at 159603074 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Stop codon at position 162 (C162*)
Ref Sequence ENSEMBL: ENSMUSP00000143475 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]
AlphaFold Q6DID7
Predicted Effect probably null
Transcript: ENSMUST00000068952
AA Change: C162*
SMART Domains Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: C162*

DomainStartEndE-ValueType
transmembrane domain 13 35 N/A INTRINSIC
low complexity region 36 47 N/A INTRINSIC
Pfam:MIG-14_Wnt-bd 178 496 3.7e-97 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000197328
Predicted Effect probably null
Transcript: ENSMUST00000198878
AA Change: C162*
SMART Domains Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: C162*

DomainStartEndE-ValueType
transmembrane domain 13 35 N/A INTRINSIC
low complexity region 36 47 N/A INTRINSIC
Pfam:MIG-14_Wnt-bd 177 497 2.8e-94 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000200191
Predicted Effect noncoding transcript
Transcript: ENSMUST00000200571
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.6%
  • 10x: 97.0%
  • 20x: 94.9%
Validation Efficiency 99% (69/70)
MGI Phenotype PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ano9 A G 7: 140,687,727 (GRCm39) I267T probably damaging Het
Anxa6 T A 11: 54,896,654 (GRCm39) N168I probably benign Het
Apbb1ip A G 2: 22,709,717 (GRCm39) probably benign Het
Aqr G A 2: 113,961,092 (GRCm39) H723Y probably damaging Het
Bbx C T 16: 50,100,755 (GRCm39) E47K probably benign Het
Car13 A G 3: 14,721,357 (GRCm39) probably benign Het
Casp9 T A 4: 141,534,484 (GRCm39) I298N possibly damaging Het
Ccdc66 T C 14: 27,220,430 (GRCm39) E261G probably damaging Het
Cep192 T A 18: 67,951,954 (GRCm39) Y376* probably null Het
Cped1 T A 6: 22,222,545 (GRCm39) probably benign Het
Ctbp2 A T 7: 132,601,073 (GRCm39) S563R possibly damaging Het
Ctdp1 A G 18: 80,490,637 (GRCm39) probably null Het
Dgka G C 10: 128,556,952 (GRCm39) probably benign Het
Drd2 A G 9: 49,311,084 (GRCm39) T112A probably benign Het
Dusp1 A G 17: 26,727,143 (GRCm39) V52A probably damaging Het
Eea1 T A 10: 95,875,634 (GRCm39) probably benign Het
Etfrf1 T C 6: 145,161,288 (GRCm39) V86A probably benign Het
Fbn1 A T 2: 125,163,596 (GRCm39) C2087S possibly damaging Het
Fosb T G 7: 19,041,075 (GRCm39) R139S probably damaging Het
Foxm1 C T 6: 128,349,566 (GRCm39) R362W probably damaging Het
Frem2 A G 3: 53,561,381 (GRCm39) V1042A probably damaging Het
Gbe1 A G 16: 70,280,802 (GRCm39) H401R probably benign Het
Gm10549 C T 18: 33,597,235 (GRCm39) probably benign Het
Golga7b A T 19: 42,251,758 (GRCm39) probably benign Het
H2-DMb1 T C 17: 34,378,399 (GRCm39) V235A probably benign Het
Hr A G 14: 70,793,916 (GRCm39) T59A probably benign Het
Itpr2 C A 6: 146,260,890 (GRCm39) A588S probably benign Het
Kmt2c G A 5: 25,514,706 (GRCm39) P3046S probably damaging Het
Lamc1 G A 1: 153,126,811 (GRCm39) probably benign Het
Lrp2 A G 2: 69,260,651 (GRCm39) Y4527H probably damaging Het
Map3k2 G A 18: 32,345,226 (GRCm39) probably null Het
Mroh2a C A 1: 88,170,142 (GRCm39) A685D possibly damaging Het
Nfs1 C G 2: 155,974,580 (GRCm39) G212R probably damaging Het
Nol8 C T 13: 49,815,923 (GRCm39) A677V possibly damaging Het
Nrap T A 19: 56,340,054 (GRCm39) Y740F probably damaging Het
Nup205 T A 6: 35,185,772 (GRCm39) M859K probably damaging Het
Nxf1 T C 19: 8,745,103 (GRCm39) F451S possibly damaging Het
Or5an1c A T 19: 12,218,505 (GRCm39) N173K probably damaging Het
Or6c6c G A 10: 129,541,516 (GRCm39) M256I probably benign Het
Pcdhac2 T C 18: 37,278,720 (GRCm39) Y567H probably damaging Het
Phlpp2 C T 8: 110,634,145 (GRCm39) R242W probably damaging Het
Pi4ka A G 16: 17,100,796 (GRCm39) probably benign Het
Pmpcb A G 5: 21,953,829 (GRCm39) D359G probably damaging Het
Poll T G 19: 45,546,309 (GRCm39) S244R probably benign Het
Prkd3 T C 17: 79,264,644 (GRCm39) D657G probably null Het
Prune2 G A 19: 17,098,274 (GRCm39) M1259I probably benign Het
Ptpra T A 2: 130,379,541 (GRCm39) M329K probably damaging Het
Rbm10 GGGAGGAGGAGGAGGAGGAGGATGAGGAGGAGGAGGAGGAG GGGAGGAGGAGGAGGAGGATGAGGAGGAGGAGGAGGAG X: 20,503,798 (GRCm39) probably benign Het
Rbm15 G T 3: 107,237,880 (GRCm39) D839E probably damaging Het
Sap30bp T A 11: 115,855,103 (GRCm39) I271N probably damaging Het
Scart2 T A 7: 139,828,874 (GRCm39) C178S probably damaging Het
Scn3a A T 2: 65,338,918 (GRCm39) V587E probably damaging Het
Setdb1 A T 3: 95,232,164 (GRCm39) probably benign Het
Sgk3 T A 1: 9,949,306 (GRCm39) probably null Het
Shox2 A T 3: 66,881,184 (GRCm39) H265Q probably damaging Het
Slc9a2 T C 1: 40,783,017 (GRCm39) F427S possibly damaging Het
Smarca5 T A 8: 81,463,360 (GRCm39) Q69H probably benign Het
Specc1l T A 10: 75,084,293 (GRCm39) F672Y probably damaging Het
Ssh2 T A 11: 77,298,969 (GRCm39) S105R probably damaging Het
Syne2 C T 12: 75,968,000 (GRCm39) R917* probably null Het
Tbc1d2 G A 4: 46,649,913 (GRCm39) T41M possibly damaging Het
Tbx18 T A 9: 87,606,408 (GRCm39) I246F probably damaging Het
Tcf4 T A 18: 69,814,883 (GRCm39) probably benign Het
Tmed2 C A 5: 124,679,502 (GRCm39) probably null Het
Tmem243 A T 5: 9,151,361 (GRCm39) D15V possibly damaging Het
Vmn2r87 T A 10: 130,307,848 (GRCm39) S797C probably damaging Het
Vps13c T A 9: 67,793,528 (GRCm39) probably benign Het
Zbtb7c C T 18: 76,270,464 (GRCm39) T184I probably benign Het
Zc3h13 A G 14: 75,546,405 (GRCm39) K169E probably damaging Het
Other mutations in Wls
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01924:Wls APN 3 159,607,080 (GRCm39) nonsense probably null
IGL02065:Wls APN 3 159,616,993 (GRCm39) missense probably damaging 0.99
IGL02073:Wls APN 3 159,612,890 (GRCm39) critical splice donor site probably null
IGL02133:Wls APN 3 159,603,007 (GRCm39) missense probably damaging 1.00
R0561:Wls UTSW 3 159,578,705 (GRCm39) missense probably benign 0.32
R1697:Wls UTSW 3 159,602,995 (GRCm39) missense probably benign 0.12
R1791:Wls UTSW 3 159,617,450 (GRCm39) missense probably benign 0.17
R2444:Wls UTSW 3 159,612,867 (GRCm39) missense probably damaging 1.00
R3161:Wls UTSW 3 159,603,073 (GRCm39) missense probably damaging 1.00
R4285:Wls UTSW 3 159,639,902 (GRCm39) missense probably benign
R4468:Wls UTSW 3 159,578,564 (GRCm39) missense probably damaging 0.96
R4472:Wls UTSW 3 159,603,020 (GRCm39) missense probably benign 0.01
R4782:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R4799:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R4809:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R5006:Wls UTSW 3 159,617,428 (GRCm39) missense possibly damaging 0.68
R5212:Wls UTSW 3 159,578,645 (GRCm39) missense probably benign 0.15
R5434:Wls UTSW 3 159,639,976 (GRCm39) missense probably damaging 0.97
R5694:Wls UTSW 3 159,545,624 (GRCm39) missense probably benign 0.01
R6315:Wls UTSW 3 159,640,007 (GRCm39) critical splice donor site probably null
R7069:Wls UTSW 3 159,639,965 (GRCm39) missense probably damaging 1.00
R7243:Wls UTSW 3 159,615,402 (GRCm39) missense possibly damaging 0.49
R7529:Wls UTSW 3 159,578,644 (GRCm39) missense probably benign 0.43
R7697:Wls UTSW 3 159,616,955 (GRCm39) missense probably benign 0.21
R7842:Wls UTSW 3 159,578,816 (GRCm39) missense probably benign 0.09
R8136:Wls UTSW 3 159,578,761 (GRCm39) missense probably damaging 1.00
R8536:Wls UTSW 3 159,578,748 (GRCm39) missense probably damaging 0.96
R8816:Wls UTSW 3 159,639,928 (GRCm39) missense possibly damaging 0.89
R9074:Wls UTSW 3 159,615,403 (GRCm39) missense possibly damaging 0.86
Predicted Primers PCR Primer
(F):5'- AGTTCTTGACTGCAAAGGGTAGGC -3'
(R):5'- CTTATGCTGGTCATGGTATTCAGAGGC -3'

Sequencing Primer
(F):5'- ATGGCATGGCCTGTGAAC -3'
(R):5'- TCCTTCAAGAGCAGCTAAGATG -3'
Posted On 2013-05-23